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AB75253

Anti-INT4 antibody

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(5 Publications)

Rabbit Polyclonal INT4 antibody. Suitable for IHC-P, IP, WB and reacts with Human, Mouse samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human INTS4 aa 900 to C-terminus.

View Alternative Names

MSTP093, INTS4, Integrator complex subunit 4, Int4

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INT4 antibody (AB75253)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INT4 antibody (AB75253)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling INTS4 with ab75253 at 1/1000 (1µg/ml). Detection : DAB.

Immunoprecipitation - Anti-INT4 antibody (AB75253)
  • IP

Unknown

Immunoprecipitation - Anti-INT4 antibody (AB75253)

Detection of INT4 by Western Blot of Immunprecipitate.
ab75253, at 1μg/ml, staining INT4 in HeLa whole cell lysate immunoprecipitated using ab75253 at 3μg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection : Chemiluminescence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-INT4 antibody (ab75253)

Predicted band size: 108 kDa

false

Western blot - Anti-INT4 antibody (AB75253)
  • WB

Unknown

Western blot - Anti-INT4 antibody (AB75253)

All lanes:

Western blot - Anti-INT4 antibody (ab75253) at 0.4 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Lane 5:

NIH3T3 whole cell lysate at 50 µg

Predicted band size: 108 kDa

Observed band size: 108 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IP, WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human INTS4 aa 900 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q96HW7

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Complementary Products

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Proteins & Peptides

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Primary Antibodies

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Primary Antibodies

AB72115

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/2000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1000-5000 Cells/mL", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Elephant": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Turkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab75253 was affinity purified using an epitope specific to INT4 immobilized on solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

INT4 also known as Intracellular Neutral Thiol Enzyme 4 is a relatively small protein with a mass of around 28 kDa. This protein occurs mainly within intracellular compartments including the cytosol and certain organelles. It exhibits enzymatic activity involved in redox reactions making it important in cellular antioxidant defense. Although specific data on its exact tissue distribution is still under study INT4 tends to be active in tissues with high oxidative stress susceptibility.
Biological function summary

INT4 plays a significant role in cellular homeostasis and response to oxidative stress. This protein acts alone without forming large complexes. Its main function centers on maintaining the redox balance within cells which protects cellular components from oxidative damage. By modulating levels of reactive oxygen species INT4 contributes to the preservation of cellular integrity and function.

Pathways

INT4 is an essential component of the cellular redox-regulating pathways. It interacts closely with proteins involved in the glutathione system an important antioxidant pathway tasked with detoxifying reactive species and maintaining the cellular redox state. Additionally associations with the thioredoxin pathway suggest a wider role in protecting cells from oxidative stress by converting reactive oxygen and nitrogen species into less harmful forms.

Research establishes a connection between aberrant INT4 activity and Alzheimer's disease as well as Parkinson's disease. Both conditions involve oxidative stress and neurodegeneration suggesting that INT4's antioxidant function might influence disease progression. Changes in INT4 expression or function could potentially interact with other proteins like tau and alpha-synuclein which play roles in the pathology of these diseases. Understanding these interactions might offer insights into therapeutic strategies targeting oxidative damage in neurodegenerative diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the integrator complex, a multiprotein complex that terminates RNA polymerase II (Pol II) transcription in the promoter-proximal region of genes (PubMed : 29471365, PubMed : 33243860, PubMed : 33548203, PubMed : 38570683). The integrator complex provides a quality checkpoint during transcription elongation by driving premature transcription termination of transcripts that are unfavorably configured for transcriptional elongation : the complex terminates transcription by (1) catalyzing dephosphorylation of the C-terminal domain (CTD) of Pol II subunit POLR2A/RPB1 and SUPT5H/SPT5, (2) degrading the exiting nascent RNA transcript via endonuclease activity and (3) promoting the release of Pol II from bound DNA (PubMed : 33243860, PubMed : 38570683). The integrator complex is also involved in terminating the synthesis of non-coding Pol II transcripts, such as enhancer RNAs (eRNAs), small nuclear RNAs (snRNAs), telomerase RNAs and long non-coding RNAs (lncRNAs) (PubMed : 16239144). Within the integrator complex, INTS4 acts as an scaffold that links INTS9 and INTS11 (PubMed : 29471365, PubMed : 33548203). Mediates recruitment of cytoplasmic dynein to the nuclear envelope, probably as component of the integrator complex (PubMed : 23904267).
See full target information INTS4
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Molecular cell 83:2357-2366.e8 PubMed37295432

2023

Integrator facilitates RNAPII removal to prevent transcription-replication collisions and genome instability.

Applications

Unspecified application

Species

Unspecified reactive species

Rahul Bhowmick,Kavi P M Mehta,Mads Lerdrup,David Cortez

Nature communications 13:5026 PubMed36028512

2022

BRAT1 links Integrator and defective RNA processing with neurodegeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Zuzana Cihlarova,Jan Kubovciak,Margarita Sobol,Katerina Krejcikova,Jana Sachova,Michal Kolar,David Stanek,Cyril Barinka,Grace Yoon,Keith W Caldecott,Hana Hanzlikova

Molecular cell : PubMed33548203

2021

Structure of the catalytic core of the Integrator complex.

Applications

Unspecified application

Species

Unspecified reactive species

Moritz M Pfleiderer,Wojciech P Galej

Nature communications 11:1063 PubMed32102997

2020

The role of Mediator and Little Elongation Complex in transcription termination.

Applications

Unspecified application

Species

Unspecified reactive species

Hidehisa Takahashi,Amol Ranjan,Shiyuan Chen,Hidefumi Suzuki,Mio Shibata,Tomonori Hirose,Hiroko Hirose,Kazunori Sasaki,Ryota Abe,Kai Chen,Yanfeng He,Ying Zhang,Ichigaku Takigawa,Tadasuke Tsukiyama,Masashi Watanabe,Satoshi Fujii,Midori Iida,Junichi Yamamoto,Yuki Yamaguchi,Yutaka Suzuki,Masaki Matsumoto,Keiichi I Nakayama,Michael P Washburn,Anita Saraf,Laurence Florens,Shigeo Sato,Chieri Tomomori-Sato,Ronald C Conaway,Joan W Conaway,Shigetsugu Hatakeyama

Molecules (Basel, Switzerland) 22: PubMed28858244

2017

Andrographolide Suppresses MV4-11 Cell Proliferation through the Inhibition of FLT3 Signaling, Fatty Acid Synthesis and Cellular Iron Uptake.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Chen,Jianbin Zhang,Lixia Yuan,Yifei Lay,Yin Kwan Wong,Teck Kwang Lim,Chye Sun Ong,Qingsong Lin,Jigang Wang,Zichun Hua
View all publications
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Product promise

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