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Rabbit Recombinant Monoclonal Integrin alpha 1 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-Integrin alpha 1 antibody [EPR27291-17] - BSA and Azide free (AB317554), expandable thumbnail
  • Western blot - Anti-Integrin alpha 1 antibody [EPR27291-17] - BSA and Azide free (AB317554), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBFlow CytICC/IFIPIHC-P
Human
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Tested
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Integrin alpha-1/beta-1 is a receptor for laminin and collagen. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Involved in anchorage-dependent, negative regulation of EGF-stimulated cell growth.

Alternative names

CD49a, Integrin alpha-1, CD49 antigen-like family member A, Laminin and collagen receptor, VLA-1, ITGA1

Recommended products

Rabbit Recombinant Monoclonal Integrin alpha 1 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR27291-17
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab317554 is the carrirer-free version of Anti-Integrin alpha 1 antibody [EPR27291-17] ab317553.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Integrin alpha 1 also called CD49a is a protein that functions as a mechanical link between cells and the extracellular matrix. It is part of the integrin protein family and partners with beta 1 integrin to form the integrin alpha 1 beta 1 complex. This protein is a heterodimer and weighs approximately 180 kDa. The integrin alpha 1 is expressed in various tissues including the dermis cartilage and the immune system especially on activated T cells and natural killer cells.

Biological function summary

Integrin alpha 1 beta 1 mediates cell adhesion and migration. It plays an important role in collagen binding influencing various cellular processes like proliferation and differentiation. Integrin alpha 1 forms a complex with ligand molecules notably collagen which assists in remodeling the extracellular matrix. This activity contributes to the development and repair of tissues offering structural support and pathways for cell signaling events.

Pathways

Integrin alpha 1 engages in the MAPK signaling pathway and regulation of the TGF-beta signaling cascade. By mediating interactions between cells and their environment it influences downstream signaling and gene expression. Integrins including integrin alpha 1 have roles in pathways related to cellular stress responses and signal modulation interacting with proteins such as fibronectin within these processes.

Associated diseases and disorders

Integrin alpha 1 has been linked to fibrosis and chronic inflammatory diseases. By modulating matrix-related signals it influences the pathology of fibrosis through upregulation of matrix metalloproteinases. In chronic inflammatory diseases its interaction with collagen and other matrix proteins can affect immune cell trafficking and signaling. Understanding these connections helps in exploring potential therapeutic targets and interventions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-Integrin alpha 1 antibody [EPR27291-17] - BSA and Azide free (ab317554), expandable thumbnail

    Western blot - Anti-Integrin alpha 1 antibody [EPR27291-17] - BSA and Azide free (ab317554)

    This data was developed using Anti-Integrin alpha 1 antibody [EPR27291-17] ab317553, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: AsPC-1, U-937, K-562, A20.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-Integrin alpha 1 antibody [EPR27291-17] (Anti-Integrin alpha 1 antibody [EPR27291-17] ab317553) at 1/1000 dilution

    Lane 1: HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 50 µg with 5% NFDM/TBST

    Lane 2: Huh7 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 50 µg with 5% NFDM/TBST

    Lane 3: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 50 µg with 5% NFDM/TBST

    Lane 4: AsPC-1 (human Pancreas epithelial cell) whole cell lysate at 50 µg with 5% NFDM/TBST

    Lane 5: U-937 (human histiocytic lymphoma monocyte) whole cell lysate at 50 µg with 5% NFDM/TBST

    Lane 6: K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 50 µg with 5% NFDM/TBST

    Lane 7: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 50 µg with 5% NFDM/TBST

    Lane 8: A20 (mouse reticulum sarcoma B lymphocyte) whole cell lysate at 50 µg with 5% NFDM/TBST

    Lane 9: PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 50 µg with 5% NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 200 kDa, 36 kDa

    Exposure time: 180s

  • Western blot - Anti-Integrin alpha 1 antibody [EPR27291-17] - BSA and Azide free (ab317554), expandable thumbnail

    Western blot - Anti-Integrin alpha 1 antibody [EPR27291-17] - BSA and Azide free (ab317554)

    This data was developed using Anti-Integrin alpha 1 antibody [EPR27291-17] ab317553, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The lanes 1, 4-5 were developed using a high sensitivity ECL substrate.

    Lanes 4-5 are applied with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 and lanes 1-3 are applied with Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-Integrin alpha 1 antibody [EPR27291-17] (Anti-Integrin alpha 1 antibody [EPR27291-17] ab317553) at 1/1000 dilution

    Lane 1: Human lymph node tissue lysate at 20 µg with 5% NFDM/TBST

    Lane 2: Human liver cancer tissue lysate at 20 µg with 5% NFDM/TBST

    Lane 3: Human placenta tissue lysate at 20 µg with 5% NFDM/TBST

    Lane 4: Mouse kidney tissue lysate at 20 µg with 5% NFDM/TBST

    Lane 5: Rat kidney tissue lysate at 20 µg with 5% NFDM/TBST

    Secondary

    Lanes 1 - 5: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Lanes 1 - 5: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 200 kDa, 36 kDa

    Exposure time: 180s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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