Anti-Integrin alpha 1 antibody [EPR27291-17] - BSA and Azide free

• WB

Supplier Data

Western blot - Anti-Integrin alpha 1 antibody [EPR27291-17] - BSA and Azide free (AB317554)

This data was developed using ab317553, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The lanes 1, 4-5 were developed using a high sensitivity ECL substrate.

Lanes 4-5 are applied with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 and lanes 1-3 are applied with Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Integrin alpha 1 antibody [EPR27291-17] (<a href='/en-us/products/primary-antibodies/integrin-alpha-1-antibody-epr27291-17-ab317553'>ab317553</a>) at 1/1000 dilution

Lane 1:

Human lymph node tissue lysate at 20 µg

Lane 2:

Human liver cancer tissue lysate at 20 µg

Lane 3:

Human placenta tissue lysate at 20 µg

Lane 4:

Mouse kidney tissue lysate at 20 µg

Lane 5:

Rat kidney tissue lysate at 20 µg

Secondary

Lanes 1 - 5:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Lanes 1 - 5:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 200 kDa,36 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-Integrin alpha 1 antibody [EPR27291-17] - BSA and Azide free (AB317554)

This data was developed using ab317553, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : AsPC-1, U-937, K-562, A20.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Integrin alpha 1 antibody [EPR27291-17] (<a href='/en-us/products/primary-antibodies/integrin-alpha-1-antibody-epr27291-17-ab317553'>ab317553</a>) at 1/1000 dilution

Lane 1:

HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 50 µg

Lane 2:

Huh7 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 50 µg

Lane 3:

HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 50 µg

Lane 4:

AsPC-1 (human Pancreas epithelial cell) whole cell lysate at 50 µg

Lane 5:

U-937 (human histiocytic lymphoma monocyte) whole cell lysate at 50 µg

Lane 6:

K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 50 µg

Lane 7:

Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 50 µg

Lane 8:

A20 (mouse reticulum sarcoma B lymphocyte) whole cell lysate at 50 µg

Lane 9:

PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 200 kDa,36 kDa

false

Exposure time: 180s