Mouse Recombinant Monoclonal Integrin alpha 2 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.
Images
![Western blot - Anti-Integrin alpha 2 antibody [2/CD49b] - BSA and Azide free (AB300629), expandable thumbnail](https://content.abcam.com/products/images/integrin-alpha-2-antibody-2-cd49b-bsa-and-azide-free-ab300629--western-blot-img401285.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Integrin alpha 2 antibody [2/CD49b] - BSA and Azide free (AB300629)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 2 antibody [2/CD49b] - BSA and Azide free (AB300629), expandable thumbnail](https://content.abcam.com/products/images/integrin-alpha-2-antibody-2-cd49b-bsa-and-azide-free-ab300629--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img401300.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 2 antibody [2/CD49b] - BSA and Azide free (AB300629)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 2 antibody [2/CD49b] - BSA and Azide free (AB300629), expandable thumbnail](https://content.abcam.com/products/images/integrin-alpha-2-antibody-2-cd49b-bsa-and-azide-free-ab300629--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img401299.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 2 antibody [2/CD49b] - BSA and Azide free (AB300629)")
![Western blot - Anti-Integrin alpha 2 antibody [2/CD49b] - BSA and Azide free (AB300629), expandable thumbnail](https://content.abcam.com/products/images/integrin-alpha-2-antibody-2-cd49b-bsa-and-azide-free-ab300629--western-blot-img401286.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Integrin alpha 2 antibody [2/CD49b] - BSA and Azide free (AB300629)")
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | ICC/IF | Flow Cyt | |
|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Integrin alpha-2/beta-1 is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix. (Microbial infection) Integrin ITGA2:ITGB1 acts as a receptor for Human rotavirus A. (Microbial infection) Integrin ITGA2:ITGB1 acts as a receptor for Human echoviruses 1 and 8.
Alternative names
CD49b, CD49B, ITGA2, Integrin alpha-2, CD49 antigen-like family member B, Collagen receptor, Platelet membrane glycoprotein Ia, VLA-2 subunit alpha, GPIa
Recommended products
Mouse Recombinant Monoclonal Integrin alpha 2 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- 2/CD49b
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Integrin alpha 2 also known as CD49b ITGA2 and A2 is a protein that functions as part of the integrin family. Integrins are transmembrane receptors that facilitate cell-extracellular matrix adhesion. Integrin alpha 2 has a molecular mass of approximately 130 kDa and is widely expressed in epithelial cells fibroblasts and platelets. This protein is important for mediating adhesion between cells and the extracellular matrix affecting cell signaling and behavior. Through this adhesion cells can communicate with their environment adjusting their functions accordingly.
- Biological function summary
Integrin alpha 2 plays a significant role in cellular processes like migration and proliferation. It forms a heterodimer complex by pairing with the beta 1-integrin subunit contributing to its adhesive and signaling functions. This association enables the integrin alpha 2 complex to bind specific extracellular matrix components such as collagen and laminin. Through these interactions it regulates processes like wound healing and tissue remodeling and affects how cells respond to mechanical stress.
- Pathways
Integrin alpha 2 is involved in major signaling routes that influence cell fate and movement. Notably it participates in the PI3K/AKT and MAPK pathways which are important for regulating cell growth and survival. It interacts with signaling proteins like focal adhesion kinase (FAK) and Src family kinases linking extracellular matrix changes to intracellular responses. These pathways help in transmitting signals that coordinate the cell's structural and functional adjustments in response to its surroundings.
- Associated diseases and disorders
Integrin alpha 2 has connections to cancer and fibrosis. In cancers its altered expression may contribute to tumor progression and metastasis often involving interactions with other integrins and matrix metalloproteinases that degrade extracellular components. In fibrosis excessive integrin alpha 2 activity can lead to abnormal tissue remodeling and scarring implicating its role alongside collagen-producing cells. Understanding these connections is vital for developing therapies targeting integrin-mediated pathways in these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-Integrin alpha 2 antibody [2/CD49b] - BSA and Azide free (ab300629), expandable thumbnail](https://content.abcam.com/products/images/integrin-alpha-2-antibody-2-cd49b-bsa-and-azide-free-ab300629--western-blot-img401285.jpg "Western blot - Anti-Integrin alpha 2 antibody [2/CD49b] - BSA and Azide free (ab300629)")
Western blot - Anti-Integrin alpha 2 antibody [2/CD49b] - BSA and Azide free (ab300629)
This data was developed using Anti-Integrin alpha 2 antibody [2/CD49b] ab300628, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBSFalse colour image of Western blot: Anti-Integrin alpha 2 antibody [2/CD49b] (Anti-Integrin alpha 2 antibody [2/CD49b] ab300628) staining at 1/1000 dilution, shown in green; Rabbit anti-GAPDH antibody [16891] (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/20000 dilution, shown in red.
In Western blot, Anti-Integrin alpha 2 antibody [2/CD49b] ab300628 was shown to bind specifically to ITGA2. A band was observed at 150 kDa in wild-type HeLa cell lysates with no signal observed at this size in the ITGA2 knockout cell line. To generate this image, wild-type and ITGA2 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a PVDF-FL membrane. Membranes were blocked in Odyssey diluted in equal volume of 0.1 % TBS before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat Anti-Mouse IgG H&L (IRDye® 800CW) (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) at 1/10000 dilution.
Performed under reducing conditions.
Exposure time:All lanes: Western blot - Anti-Integrin alpha 2 antibody [2/CD49b] (Anti-Integrin alpha 2 antibody [2/CD49b] ab300628) at 1/1000 dilution
Lane 1: Wild-type HeLa (human cervix adenocarcinoma epithelial cell) cell lysate
Lane 2: ITGA2 knockout HeLa (human cervix adenocarcinoma epithelial cell) cell lysate
Lane 3: A431 (human epidermoid carcinoma epithelial cell) cell lysate
SecondaryLanes 1 - 3: Western blot - Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) at 1/10000 dilution
Lanes 1 - 3: Western blot - Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) at 1/10000 dilution
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 2 antibody [2/CD49b] - BSA and Azide free (ab300629), expandable thumbnail](https://content.abcam.com/products/images/integrin-alpha-2-antibody-2-cd49b-bsa-and-azide-free-ab300629--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img401300.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 2 antibody [2/CD49b] - BSA and Azide free (ab300629)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 2 antibody [2/CD49b] - BSA and Azide free (ab300629)
This data was developed using Anti-Integrin alpha 2 antibody [2/CD49b] ab300628, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue labeling Integrin alpha 2 with Anti-Integrin alpha 2 antibody [2/CD49b] ab300628 at 1/2000 (0.516 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Positive staining on human cervical cancer.The section was incubated with Anti-Integrin alpha 2 antibody [2/CD49b] ab300628 for 30 mins at room temperature, followed by anti-mouse IgG2a antibody for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 2 antibody [2/CD49b] - BSA and Azide free (ab300629), expandable thumbnail](https://content.abcam.com/products/images/integrin-alpha-2-antibody-2-cd49b-bsa-and-azide-free-ab300629--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img401299.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 2 antibody [2/CD49b] - BSA and Azide free (ab300629)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 2 antibody [2/CD49b] - BSA and Azide free (ab300629)
This data was developed using Anti-Integrin alpha 2 antibody [2/CD49b] ab300628, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Integrin alpha 2 with Anti-Integrin alpha 2 antibody [2/CD49b] ab300628 at 1/2000 (0.516 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Positive staining on human colon.The section was incubated with Anti-Integrin alpha 2 antibody [2/CD49b] ab300628 for 30 mins at room temperature, followed by anti-mouse IgG2a antibody for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins![Western blot - Anti-Integrin alpha 2 antibody [2/CD49b] - BSA and Azide free (ab300629), expandable thumbnail](https://content.abcam.com/products/images/integrin-alpha-2-antibody-2-cd49b-bsa-and-azide-free-ab300629--western-blot-img401286.jpg "Western blot - Anti-Integrin alpha 2 antibody [2/CD49b] - BSA and Azide free (ab300629)")
Western blot - Anti-Integrin alpha 2 antibody [2/CD49b] - BSA and Azide free (ab300629)
This data was developed using Anti-Integrin alpha 2 antibody [2/CD49b] ab300628, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: Lanes 1-2: 26 seconds
Lane 3: 15 secondsAll lanes: Western blot - Anti-Integrin alpha 2 antibody [2/CD49b] (Anti-Integrin alpha 2 antibody [2/CD49b] ab300628) at 1/1000 dilution
Lane 1: A549 (human lu carcinoma epithelial cell), whole cell lysate 10
Lane 2: 293T (human embryonic kidney epithelial cell), whole cell lysate
Lane 3: U-2 OS (human bone osteosarcoma epithelial cell), whole cell lysate
SecondaryAll lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
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