Name: Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal
SKU: ab181548
Rating: 5 (4 reviews)

Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (ab181548) is a rabbit monoclonal antibody that is used to detect Integrin alpha 2 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat samples.

- Specificity confirmed with Integrin alpha 2 knockout cell line validation

Related conjugates and formulations

ab208770
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Alexa Fluor® 488 Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal
ab210655
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Alexa Fluor® 594 Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal
ab209944
Conjugated
HRP Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal
ab280853
Conjugated
Alexa Fluor® 555 Anti-Integrin alpha 2 antibody [EPR17338]
ab225284
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PE Anti-Integrin alpha 2 antibody [EPR17338]
ab209766
Conjugated
Alexa Fluor® 647 Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal
ab271936
Carrier free
Anti-Integrin alpha 2 antibody [EPR17338] - BSA and Azide free

Images

Western blot - Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (AB181548), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	WB	ICC/IF	Flow Cyt (Intra)	IHC-P
Human	Tested	Tested	Tested	Tested	Tested
Mouse	Expected	Tested	Expected	Expected	Tested
Rat	Expected	Tested	Expected	Expected	Tested

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/150	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/5000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/5000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/5000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1 µg/mL	Notes This product gave a positive signal in wild-type HAP1 cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min).

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/160	Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/500	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/500	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/500	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

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Target data

See full target information ITGA2

Function

Integrin alpha-2/beta-1 is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix. (Microbial infection) Integrin ITGA2:ITGB1 acts as a receptor for Human rotavirus A. (Microbial infection) Integrin ITGA2:ITGB1 acts as a receptor for Human echoviruses 1 and 8.

Alternative names

CD49b, CD49B, ITGA2, Integrin alpha-2, CD49 antigen-like family member B, Collagen receptor, Platelet membrane glycoprotein Ia, VLA-2 subunit alpha, GPIa

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR17338
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

What is this antibody validated in?

Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (ab181548) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), in Human, Mouse, Rat samples.

What is the molecular weight of Integrin alpha 2?

Anti-Integrin alpha 2 [EPR17338] - C-terminal (ab181548) specifically detects a band for Integrin alpha 2 (UniProt: P17301) at a molecular weight of 129kDa.

Recommended positive controls

WB: A549, A431, 293T,T-47D, C6 and NIH/3T3 whole cell lysates, human fetal brain and fetal heart, mouse heart and kidney, and rat spleen tissue lysates.IHC-P: Human colon, human squamous cell carcinoma of cervix, mouse kidney and rat colon tissues.ICC/IF: Wild-type HAP1, PC-3 and MCF7 cells.Flow Cyt (intra): A549 cells.IP: T-47D whole cell extract.

Trusted by the scientific community

Anti-Integrin alpha 2 [EPR17338] - C-terminal (ab181548) was first used in a scientific publication in 2014 and has been cited over 20 times in peer-reviewed journals.

Trial sizes available!

Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies. Specificity confirmed

The specificity of Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (ab181548) has been confirmed by Western blot testing in ITGA2 Knockout HAP1 cells.

Other related products

We have a range of other formats of antibody clone [EPR17338] also available for your convenience:
ab181548, Alexa Fluor® 488 - ab208770, Alexa Fluor® 647 - ab209766, HRP - ab209944, Alexa Fluor® 594 - ab210655, Carrier free - ab222377, PE - ab225284, Carrier free - ab271936, Alexa Fluor® 555 - ab280853, FITC - ab322294

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Integrin alpha 2 also known as CD49b ITGA2 and A2 is a protein that functions as part of the integrin family. Integrins are transmembrane receptors that facilitate cell-extracellular matrix adhesion. Integrin alpha 2 has a molecular mass of approximately 130 kDa and is widely expressed in epithelial cells fibroblasts and platelets. This protein is important for mediating adhesion between cells and the extracellular matrix affecting cell signaling and behavior. Through this adhesion cells can communicate with their environment adjusting their functions accordingly.

Biological function summary

Integrin alpha 2 plays a significant role in cellular processes like migration and proliferation. It forms a heterodimer complex by pairing with the beta 1-integrin subunit contributing to its adhesive and signaling functions. This association enables the integrin alpha 2 complex to bind specific extracellular matrix components such as collagen and laminin. Through these interactions it regulates processes like wound healing and tissue remodeling and affects how cells respond to mechanical stress.

Pathways

Integrin alpha 2 is involved in major signaling routes that influence cell fate and movement. Notably it participates in the PI3K/AKT and MAPK pathways which are important for regulating cell growth and survival. It interacts with signaling proteins like focal adhesion kinase (FAK) and Src family kinases linking extracellular matrix changes to intracellular responses. These pathways help in transmitting signals that coordinate the cell's structural and functional adjustments in response to its surroundings.

Associated diseases and disorders

Integrin alpha 2 has connections to cancer and fibrosis. In cancers its altered expression may contribute to tumor progression and metastasis often involving interactions with other integrins and matrix metalloproteinases that degrade extracellular components. In fibrosis excessive integrin alpha 2 activity can lead to abnormal tissue remodeling and scarring implicating its role alongside collagen-producing cells. Understanding these connections is vital for developing therapies targeting integrin-mediated pathways in these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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14 product images

Western blot - Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (ab181548)
Lanes 1, 5 and 9: Wild-type HAP1 cell lysate (20 μg)
Lanes 2, 6 and 10: Integrin alpha 2 knockout HAP1 cell lysate (20 μg)
Lanes 3, 7 and 11: A431 cell lysate (20 μg)
Lanes 4, 8 and 12: T47D cell lysate (20 μg)
Lanes 1, 2, 3 and 4: Green signal from target - ab181548 observed at 150 kDa
Lanes 5, 6, 7 and 8: Red signal from loading control - Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa
Lanes 9, 10, 11 and 12: Merged (red and green) signal
ab181548 was shown to specifically react with Integrin alpha 2 when Integrin alpha 2 knockout samples were used. Wild-type and Integrin alpha 2 knockout samples were subjected to SDS-PAGE. ab181548 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were diluted 1/5000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
All lanes: Western blot - Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (ab181548)
Predicted band size: 129 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (ab181548)
Immunohistochemical analysis of paraffin-embedded Human squamous cell carcinoma of cervix tissue labeling Integrin alpha 2 with ab181548 at 1/500 dilution followed by Goat Anti-Rabbit HRP (IgG H&L) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Membrane and weak cytoplasmic staining on epithelial cells of human squamous cell carcinoma of cervix tissue is observed. Counter stained with Hematoxylin.

Negative control: Used PBS instead of primary antibody.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (ab181548)
ab181548 staining Integrin α2 in wild-type HAP1 cells (top panel) and Integrin α2 knockout HAP1 cells (bottom panel). The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab181548 at 1μg/ml concentration and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 at 1ug/ml concentration overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to Rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 2 μg/ml (shown in green) and a goat secondary antibody to Mouse IgG (Alexa Fluor® 594) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 2ug/ml (shown in pseudo-color red). Nuclear DNA was labelled in blue with DAPI.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Western blot - Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (ab181548)
Blocking and diluting buffer 5% NFDM/TBST.
The increased molecular mass observed is due to glycosylation.
All lanes: Western blot - Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (ab181548) at 1/20000 dilution
Lane 1: A549 (Human lung carcinoma) whole cell lysates at 20 µg
Lane 2: A431 (Human epidermoid carcinoma) whole cell lysates at 20 µg
Lane 3: 293T (Human epithelial cells from embryonic kidney) whole cell lysates at 20 µg
Lane 4: T-47D (Human ductal breast epithelial tumor cell line) whole cell lysates at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 129 kDa
Observed band size: 150 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (ab181548)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Integrin alpha 2 with ab181548 at 1/500 dilution followed by Goat Anti-Rabbit HRP (IgG H&L) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Membrane and weak cytoplasmic staining on epithelial cells of human colon is observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (ab181548)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling integrin alpha 2 with ab181548 at 1/100 dilution, followed by Goat anti-rabbit IAlexa Fluor® 488 (IgG) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/400 dilution (green). Confocal image showing membrane staining on MCF7 cell line is observed. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (goat anti-mouse AlexaFluor®594 secondary antibody) at 1/500 dilution (red).
The negative controls are as follows:-
-ve control 1 - ab181548 at 1/100 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2. - Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.
Western blot - Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (ab181548)
Blocking and diluting buffer 5% NFDM/TBST.
The increased molecular mass observed is due to glycosylation.
All lanes: Western blot - Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (ab181548) at 1/5000 dilution
Lane 1: Mouse heart tissue lysate at 10 µg
Lane 2: Mouse kidney tissue lysate at 10 µg
Lane 3: Rat spleen tissue lysate at 10 µg
Lane 4: C6 (Rat glial tumor cells) whole cell lysate at 10 µg
Lane 5: NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate at 10 µg
Secondary
All lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 129 kDa
Observed band size: 150 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (ab181548)
Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Integrin alpha 2 with ab181548 at 1/500 dilution followed by Goat Anti-Rabbit HRP (IgG H&L) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Membrane and weak cytoplasmic staining on epithelial cells of Mouse kidney tubule is observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (ab181548)
Integrin alpha 2 was immunoprecipitated from 1mg of T-47D (Human ductal breast epithelial tumor cell line) whole cell extract with ab181548 at 1/150 dilution. Western blot was performed using ab181548 at 1/20,000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1: T-47D whole cell extract Lane 2: PBS instead of T-47D whole cell extract.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (ab181548)
Predicted band size: 129 kDa
Observed band size: 150 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (ab181548)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized PC-3 (Human prostate adenocarcinoma cell line) cells labeling integrin alpha 2 with ab181548 at 1/100 dilution, followed by Goat anti-rabbit IAlexa Fluor® 488 (IgG) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/400 dilution (green). Confocal image showing membrane and weakly cytoplasmic staining on PC-3 cell line is observed. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (goat anti-mouse AlexaFluor®594 secondary antibody) at 1/500 dilution (red).
The negative controls are as follows:-
-ve control 1 - ab181548 at 1/100 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2. - Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.
Flow Cytometry (Intracellular) - Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (ab181548)
Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed A549 (Human lung carcinoma) cells labeling integrin alpha 2 with Anti-Integrin alpha 2 antibody [EPR17349] ab181549 at 1/160 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and a unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (ab181548)
Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling Integrin alpha 2 with ab181548 at 1/500 dilution followed by Goat Anti-Rabbit HRP (IgG H&L) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Membrane staining on epithelial cells of Rat colon tissue is observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (ab181548)
Blocking and diluting buffer 5% NFDM/TBST.
The increased molecular mass observed is due to glycosylation.
All lanes: Western blot - Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (ab181548) at 1/5000 dilution
Lane 1: Human fetal brain whole cell lysates at 10 µg
Lane 2: Human fetal heart whole cell lysates at 10 µg
Secondary
All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 129 kDa
Observed band size: 150 kDa
Flow Cytometry (Intracellular) - Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (ab181548)
Flow cytometry overlay histogram showing staining with ab181548 of HAP1 WT positive cells (magenta line) and HAP1-ITGA2 KO negative cells (red line). The cells were fixed with 80% methanol, and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab181548) (1x 10⁶ in 100μl at 0.2μg/ml (1/2,500)) for 30min at 22°C.
The secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed was incubated at 1/4000 for 30min at 22°C
Isotype control antibody was Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control in HAP1 WT cells (black line) and HAP1-ITGA2 KO cells (grey line), used at the same concentration and conditions as the primary antibody.
Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.