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Rabbit Recombinant Monoclonal Integrin alpha 2 antibody. Suitable for IP, WB and reacts with Human samples.

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Images

Western blot - Anti-Integrin alpha 2 antibody [EPR17349] (AB181549), expandable thumbnail
  • Western blot - Anti-Integrin alpha 2 antibody [EPR17349] (AB181549), expandable thumbnail
  • Western blot - Anti-Integrin alpha 2 antibody [EPR17349] (AB181549), expandable thumbnail
  • Western blot - Anti-Integrin alpha 2 antibody [EPR17349] (AB181549), expandable thumbnail
  • Immunoprecipitation - Anti-Integrin alpha 2 antibody [EPR17349] (AB181549), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWB
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Associated Products

Select an associated product type

15 products for Alternative Product

Target data

Function

Integrin alpha-2/beta-1 is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix. (Microbial infection) Integrin ITGA2:ITGB1 acts as a receptor for Human rotavirus A. (Microbial infection) Integrin ITGA2:ITGB1 acts as a receptor for Human echoviruses 1 and 8.

Alternative names

CD49b, CD49B, ITGA2, Integrin alpha-2, CD49 antigen-like family member B, Collagen receptor, Platelet membrane glycoprotein Ia, VLA-2 subunit alpha, GPIa

Recommended products

Rabbit Recombinant Monoclonal Integrin alpha 2 antibody. Suitable for IP, WB and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR17349
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Integrin alpha 2 also known as CD49b ITGA2 and A2 is a protein that functions as part of the integrin family. Integrins are transmembrane receptors that facilitate cell-extracellular matrix adhesion. Integrin alpha 2 has a molecular mass of approximately 130 kDa and is widely expressed in epithelial cells fibroblasts and platelets. This protein is important for mediating adhesion between cells and the extracellular matrix affecting cell signaling and behavior. Through this adhesion cells can communicate with their environment adjusting their functions accordingly.

Biological function summary

Integrin alpha 2 plays a significant role in cellular processes like migration and proliferation. It forms a heterodimer complex by pairing with the beta 1-integrin subunit contributing to its adhesive and signaling functions. This association enables the integrin alpha 2 complex to bind specific extracellular matrix components such as collagen and laminin. Through these interactions it regulates processes like wound healing and tissue remodeling and affects how cells respond to mechanical stress.

Pathways

Integrin alpha 2 is involved in major signaling routes that influence cell fate and movement. Notably it participates in the PI3K/AKT and MAPK pathways which are important for regulating cell growth and survival. It interacts with signaling proteins like focal adhesion kinase (FAK) and Src family kinases linking extracellular matrix changes to intracellular responses. These pathways help in transmitting signals that coordinate the cell's structural and functional adjustments in response to its surroundings.

Associated diseases and disorders

Integrin alpha 2 has connections to cancer and fibrosis. In cancers its altered expression may contribute to tumor progression and metastasis often involving interactions with other integrins and matrix metalloproteinases that degrade extracellular components. In fibrosis excessive integrin alpha 2 activity can lead to abnormal tissue remodeling and scarring implicating its role alongside collagen-producing cells. Understanding these connections is vital for developing therapies targeting integrin-mediated pathways in these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-Integrin alpha 2 antibody [EPR17349] (ab181549), expandable thumbnail

    Western blot - Anti-Integrin alpha 2 antibody [EPR17349] (ab181549)

    Lanes 1-4: Merged signal (red and green). Green - ab181549 observed at 160 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa.

    ab181549 Anti-Integrin alpha 2 antibody [EPR17349] was shown to specifically react with Integrin alpha 2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human ITGA2 (CD49b) knockout HeLa cell line ab264819 (knockout cell lysate Human ITGA2 (CD49b) knockout HeLa cell lysate ab257262) was used. Wild-type and Integrin alpha 2 knockout samples were subjected to SDS-PAGE. ab181549 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 Dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Integrin alpha 2 antibody [EPR17349] (ab181549) at 1/1000 dilution

    Lane 1: Wild-type HeLa cell lysate at 20 µg

    Lane 2: ITGA2 knockout HeLa cell lysate at 20 µg

    Lane 2: Western blot - Human ITGA2 (CD49b) knockout HeLa cell line (Human ITGA2 (CD49b) knockout HeLa cell line ab264819)

    Lane 3: A431 cell lysate at 20 µg

    Lane 4: K562 cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 129 kDa, 130 kDa, 171 kDa

    Observed band size: 160 kDa, 250 kDa

  • Western blot - Anti-Integrin alpha 2 antibody [EPR17349] (ab181549), expandable thumbnail

    Western blot - Anti-Integrin alpha 2 antibody [EPR17349] (ab181549)

    Lanes 1 - 3: Merged signal (red and green). Green - ab181549 observed at 160 kDa. Red - loading control, Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484, observed at 37 kDa.

    ab181549 was shown to specifically recognize ITGA2 in wild-type HAP1 cells in addition to additional cross-reactive bands. No band was observed when ITGA2 knockout samples were examined. Wild-type and ITGA2 knockout samples were subjected to SDS-PAGE. ab181549 and Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Integrin alpha 2 antibody [EPR17349] (ab181549) at 1/1000 dilution

    Lane 1: Wild-type HAP1 whole cell lysate at 20 µg

    Lane 2: ITGA2 knockout HAP1 whole cell lysate at 20 µg

    Lane 3: A431 whole cell lysate at 20 µg

    Predicted band size: 129 kDa

  • Western blot - Anti-Integrin alpha 2 antibody [EPR17349] (ab181549), expandable thumbnail

    Western blot - Anti-Integrin alpha 2 antibody [EPR17349] (ab181549)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The increased of molecular weight is due to glycosylation and is consistent with what has been described in the literature (PMID: 10825287 & 22354032).

    All lanes: Western blot - Anti-Integrin alpha 2 antibody [EPR17349] (ab181549) at 1/1000 dilution

    Lane 1: A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

    Lane 2: A431 (Human epidermoid carcinoma cell line) whole cell lysate at 20 µg

    Lane 3: PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate at 20 µg

    Lane 4: MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 129 kDa

    Observed band size: 150 kDa

    Exposure time: 30s

  • Western blot - Anti-Integrin alpha 2 antibody [EPR17349] (ab181549), expandable thumbnail

    Western blot - Anti-Integrin alpha 2 antibody [EPR17349] (ab181549)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The increased of molecular weight is due to glycosylation and is consistent with what has been described in the literature (PMID: 10825287 & 22354032).

    All lanes: Western blot - Anti-Integrin alpha 2 antibody [EPR17349] (ab181549) at 1/1000 dilution

    All lanes: Human kidney lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 129 kDa

    Observed band size: 150 kDa

    Exposure time: 3min

  • Immunoprecipitation - Anti-Integrin alpha 2 antibody [EPR17349] (ab181549), expandable thumbnail

    Immunoprecipitation - Anti-Integrin alpha 2 antibody [EPR17349] (ab181549)

    Integrin alpha 2 was immunoprecipitated from 0.35 mg of A549 (Human lung carcinoma cell line) whole cell lysate with ab181549 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab181549 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.

    Lane 1: A549 whole cell lysate, 10μg (Input).

    Lane 2: ab181549 IP in A549 whole cell lysate.

    Lane 3: Rabbit IgG,monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab181549 in A549 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

    All lanes: Immunoprecipitation - Anti-Integrin alpha 2 antibody [EPR17349] (ab181549)

    Predicted band size: 129 kDa

  • Western blot - Anti-Integrin alpha 2 antibody [EPR17349] (ab181549), expandable thumbnail

    Western blot - Anti-Integrin alpha 2 antibody [EPR17349] (ab181549)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The increased of molecular weight is due to glycosylation and is consistent with what has been described in the literature (PMID: 10825287 & 22354032).

    All lanes: Western blot - Anti-Integrin alpha 2 antibody [EPR17349] (ab181549) at 1/5000 dilution

    All lanes: Human skin lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/10000 dilution

    Predicted band size: 129 kDa

    Observed band size: 150 kDa

    Exposure time: 3min

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Product protocols

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