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AB323184

Anti-Integrin alpha 2 antibody [EPR29247-536]

  • Recombinant
  • KO Validated
  • 20ul selling size
  • RabMAb
  • What is this?

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Rabbit Recombinant Monoclonal Integrin alpha 2 antibody. Suitable for Flow Cyt and reacts with Human samples.

View Alternative Names

CD49b, CD49B, ITGA2, Integrin alpha-2, CD49 antigen-like family member B, Collagen receptor, Platelet membrane glycoprotein Ia, VLA-2 subunit alpha, GPIa

3 Images
Flow Cytometry - Anti-Integrin alpha 2 antibody [EPR29247-536] (AB323184)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-Integrin alpha 2 antibody [EPR29247-536] (AB323184)

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labeling Integrin alpha 2 with ab323184 at 1/50 dilution (1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control.

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Human PBMC are co-stained with CD14 conjugated BV421.
Gated on viable cell.

Flow Cytometry - Anti-Integrin alpha 2 antibody [EPR29247-536] (AB323184)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-Integrin alpha 2 antibody [EPR29247-536] (AB323184)

Flow cytometric analysis of Parental HeLa (human cervical adenocarcinoma epithelial cell) (Green)/ ITGA2 KO (human Integrin alpha-2 knock out) HeLa (Magenta) cells labeling Integrin alpha 2 with ab323184 at 1/500 dilution (0.1ug) / Magenta and Green compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) (Black) and (Grey) isotype control.

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells.

Flow Cytometry - Anti-Integrin alpha 2 antibody [EPR29247-536] (AB323184)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-Integrin alpha 2 antibody [EPR29247-536] (AB323184)

Flow cytometric analysis of A549 (human lung carcinoma epithelial cell) (Right) / Daudi (human Burkitt's lymphoma lymphoblast) (Left) cells labeling Integrin alpha 2 with ab323184 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells.
Negative control : Daudi.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR29247-536

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Assay Kits

AB323925

Human Integrin alpha 2 ELISA Kit

SimpleStep|Recombinant|KO Validated

Sandwich ELISA - Human Integrin alpha 2 ELISA Kit (AB323925)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/50 - 1/500", "FlowCyt-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Integrin alpha-2/beta-1 is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix.. (Microbial infection) Integrin ITGA2 : ITGB1 acts as a receptor for Human rotavirus A.. (Microbial infection) Integrin ITGA2 : ITGB1 acts as a receptor for Human echoviruses 1 and 8.
See full target information ITGA2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com