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Rabbit Recombinant Monoclonal Integrin alpha 2 antibody. Suitable for Flow Cyt and reacts with Human samples.

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Images

Flow Cytometry - Anti-Integrin alpha 2 antibody [EPR29247-536] (AB323184), expandable thumbnail
  • Flow Cytometry - Anti-Integrin alpha 2 antibody [EPR29247-536] (AB323184), expandable thumbnail
  • Flow Cytometry - Anti-Integrin alpha 2 antibody [EPR29247-536] (AB323184), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt
Human
Tested
Mouse
Not recommended

Tested
Tested

Species

Human

Dilution info
1/50 - 1/500
Notes

-

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

-

Target data

Function

Integrin alpha-2/beta-1 is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix.(Microbial infection) Integrin ITGA2:ITGB1 acts as a receptor for Human rotavirus A.(Microbial infection) Integrin ITGA2:ITGB1 acts as a receptor for Human echoviruses 1 and 8.

Alternative names

CD49b, CD49B, ITGA2, Integrin alpha-2, CD49 antigen-like family member B, Collagen receptor, Platelet membrane glycoprotein Ia, VLA-2 subunit alpha, GPIa

Rabbit Recombinant Monoclonal Integrin alpha 2 antibody. Suitable for Flow Cyt and reacts with Human samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Clone number

EPR29247-536

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Flow Cytometry - Anti-Integrin alpha 2 antibody [EPR29247-536] (ab323184), expandable thumbnail

    Flow Cytometry - Anti-Integrin alpha 2 antibody [EPR29247-536] (ab323184)

    Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labeling Integrin alpha 2 with ab323184 at 1/50 dilution (1ug) / Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control.

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

    Human PBMC are co-stained with CD14 conjugated BV421.

    Gated on viable cell.

  • Flow Cytometry - Anti-Integrin alpha 2 antibody [EPR29247-536] (ab323184), expandable thumbnail

    Flow Cytometry - Anti-Integrin alpha 2 antibody [EPR29247-536] (ab323184)

    Flow cytometric analysis of Parental HeLa (human cervical adenocarcinoma epithelial cell) (Green)/ ITGA2 KO (human Integrin alpha-2 knock out) HeLa (Magenta) cells labeling Integrin alpha 2 with ab323184 at 1/500 dilution (0.1ug) / Magenta and Green compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab199091) (Black) and (Grey) isotype control.

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

    Gated on viable cells.

  • Flow Cytometry - Anti-Integrin alpha 2 antibody [EPR29247-536] (ab323184), expandable thumbnail

    Flow Cytometry - Anti-Integrin alpha 2 antibody [EPR29247-536] (ab323184)

    Flow cytometric analysis of A549 (human lung carcinoma epithelial cell) (Right) / Daudi (human Burkitt's lymphoma lymphoblast) (Left) cells labeling Integrin alpha 2 with ab323184 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

    Gated on viable cells.

    Negative control: Daudi.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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