Name: Anti-Integrin alpha 2 antibody [EPR5788]
SKU: ab133557
Rating: 5 (5 reviews)

Anti-Integrin alpha 2 antibody [EPR5788] (ab133557) is a rabbit monoclonal antibody that is used to detect Integrin alpha 2 in Western Blot, IHC-P. Suitable for Human, Mouse samples.

- Specificity confirmed with Integrin alpha 2 knockout cell line validation

Related conjugates and formulations

ab271894
Carrier free
Anti-Integrin alpha 2 antibody [EPR5788] - BSA and Azide free
ab317897
Conjugated
PE Anti-Integrin alpha 2 antibody [EPR5788]
ab318058
Conjugated
Alexa Fluor® 555 Anti-Integrin alpha 2 antibody [EPR5788]
ab317935
Conjugated
APC Anti-Integrin alpha 2 antibody [EPR5788]
ab318016
Conjugated
Alexa Fluor® 594 Anti-Integrin alpha 2 antibody [EPR5788]
ab317975
Conjugated
Alexa Fluor® 488 Anti-Integrin alpha 2 antibody [EPR5788]
ab318100
Conjugated
Alexa Fluor® 647 Anti-Integrin alpha 2 antibody [EPR5788]
ab321053
Conjugated
Alexa Fluor® 750 Anti-Integrin alpha 2 antibody [EPR5788]

Images

Western blot - Anti-Integrin alpha 2 antibody [EPR5788] (AB133557), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	WB	ICC/IF	Flow Cyt (Intra)
Human	Tested	Tested	Not recommended	Not recommended
Mouse	Expected	Tested	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/250	Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/10000 - 1/50000	Notes -
Species Human	Dilution info 1/10000 - 1/50000	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human	Dilution info -	Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Species Mouse	Dilution info -	Notes -

Associated Products

Select an associated product type

15 products for Alternative Product

1 product for Alternative Version

Target data

See full target information ITGA2

Function

Integrin alpha-2/beta-1 is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix. (Microbial infection) Integrin ITGA2:ITGB1 acts as a receptor for Human rotavirus A. (Microbial infection) Integrin ITGA2:ITGB1 acts as a receptor for Human echoviruses 1 and 8.

Alternative names

CD49b, CD49B, ITGA2, Integrin alpha-2, CD49 antigen-like family member B, Collagen receptor, Platelet membrane glycoprotein Ia, VLA-2 subunit alpha, GPIa

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR5788
Purification technique: Affinity purification Protein A
Dissociation constant: 3.2 x 10^-12 M
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Stable for 12 months at -20°C

Notes

What is this antibody validated in?

Anti-Integrin alpha 2 antibody [EPR5788] (ab133557) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), in Human, Mouse samples.

What is the molecular weight of Integrin alpha 2?

Anti-Integrin alpha 2 [EPR5788] (ab133557) specifically detects a band for Integrin alpha 2 (UniProt: P17301) at a molecular weight of 129kDa.

Recommended positive controls

WB: T47-D, HEK293, 293T and A431 cell lysates. Human placenta and mouse spleen tissue lysates.IHC-P: Human breast carcinoma and colon tissue.

Trusted by the scientific community

Anti-Integrin alpha 2 [EPR5788] (ab133557) was first used in a scientific publication in 2012 and has been cited over 50 times in peer-reviewed journals.

Reviewed by scientists

Anti-Integrin alpha 2 [EPR5788] (ab133557) has over 5 independent reviews from customers.

Trial sizes available!

Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies. Specificity confirmed

The specificity of Anti-Integrin alpha 2 antibody [EPR5788] (ab133557) has been confirmed by Immunocytochemistry testing in ITGA2 Knockout HAP1 cells.

Other related products

We have a range of other formats of antibody clone [EPR5788] also available for your convenience:
ab133557, Carrier free - ab221214, Carrier free - ab271894, PE - ab317897, APC - ab317935, Alexa Fluor® 488 - ab317975, Alexa Fluor® 594 - ab318016, Alexa Fluor® 555 - ab318058, Alexa Fluor® 647 - ab318100, Alexa Fluor® 750 - ab321053

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Integrin alpha 2 also known as CD49b ITGA2 and A2 is a protein that functions as part of the integrin family. Integrins are transmembrane receptors that facilitate cell-extracellular matrix adhesion. Integrin alpha 2 has a molecular mass of approximately 130 kDa and is widely expressed in epithelial cells fibroblasts and platelets. This protein is important for mediating adhesion between cells and the extracellular matrix affecting cell signaling and behavior. Through this adhesion cells can communicate with their environment adjusting their functions accordingly.

Biological function summary

Integrin alpha 2 plays a significant role in cellular processes like migration and proliferation. It forms a heterodimer complex by pairing with the beta 1-integrin subunit contributing to its adhesive and signaling functions. This association enables the integrin alpha 2 complex to bind specific extracellular matrix components such as collagen and laminin. Through these interactions it regulates processes like wound healing and tissue remodeling and affects how cells respond to mechanical stress.

Pathways

Integrin alpha 2 is involved in major signaling routes that influence cell fate and movement. Notably it participates in the PI3K/AKT and MAPK pathways which are important for regulating cell growth and survival. It interacts with signaling proteins like focal adhesion kinase (FAK) and Src family kinases linking extracellular matrix changes to intracellular responses. These pathways help in transmitting signals that coordinate the cell's structural and functional adjustments in response to its surroundings.

Associated diseases and disorders

Integrin alpha 2 has connections to cancer and fibrosis. In cancers its altered expression may contribute to tumor progression and metastasis often involving interactions with other integrins and matrix metalloproteinases that degrade extracellular components. In fibrosis excessive integrin alpha 2 activity can lead to abnormal tissue remodeling and scarring implicating its role alongside collagen-producing cells. Understanding these connections is vital for developing therapies targeting integrin-mediated pathways in these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

Western blot - Anti-Integrin alpha 2 antibody [EPR5788] (ab133557)
Lane 1: Wild-type HAP1 cell lysate (20 μg)
Lane 2: Integrin alpha 2 knockout HAP1 cell lysate (20 μg)
Lane 3: A431 cell lysate (20 μg)
Lane 4: T47D cell lysate (20 μg)
Lanes 1 - 4: Merged signal (red and green). Green - ab133557 observed at 165 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.

ab133557 was shown to specifically react with Integrin alpha 2 when Integrin alpha 2 knockout samples were used. Wild-type and Integrin alpha 2 knockout samples were subjected to SDS-PAGE. ab133557 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were diluted 1/10 000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed withGoat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10,000 dilution for 1 h at room temperature before imaging.
All lanes: Western blot - Anti-Integrin alpha 2 antibody [EPR5788] (ab133557)
Predicted band size: 129 kDa
Western blot - Anti-Integrin alpha 2 antibody [EPR5788] (ab133557)
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
All lanes: Western blot - Anti-Integrin alpha 2 antibody [EPR5788] (ab133557) at 1/10000 dilution
Lane 1: T47-D cell lysate at 20 µg
Lane 2: HEK293 cell lysate at 20 µg
Secondary
All lanes: Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 129 kDa
Observed band size: 150 kDa
Western blot - Anti-Integrin alpha 2 antibody [EPR5788] (ab133557)
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
All lanes: Western blot - Anti-Integrin alpha 2 antibody [EPR5788] (ab133557) at 1/50000 dilution
All lanes: A431 cell lysate at 20 µg
Secondary
All lanes: Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 129 kDa, 162 kDa
Observed band size: 150 kDa, 162 kDa
Western blot - Anti-Integrin alpha 2 antibody [EPR5788] (ab133557)
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
All lanes: Western blot - Anti-Integrin alpha 2 antibody [EPR5788] (ab133557) at 1/10000 dilution
All lanes: Mouse spleen tissue lysate at 20 µg
Secondary
All lanes: Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 129 kDa
Observed band size: 150 kDa
Western blot - Anti-Integrin alpha 2 antibody [EPR5788] (ab133557)
All lanes: Western blot - Anti-Integrin alpha 2 antibody [EPR5788] (ab133557) at 1/10000 dilution
Lane 1: T47D lysate at 10 µg
Lane 2: 293T lysate at 10 µg
Lane 3: Human platelet lysate at 10 µg
Lane 4: A431 lysate at 10 µg
Secondary
All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 129 kDa
Observed band size: 150 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 2 antibody [EPR5788] (ab133557)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling Integrin alpha 2 with purified ab133557 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 2 antibody [EPR5788] (ab133557)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labelling Integrin alpha 2 with unpurified ab133557 at 1/250.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cytometry (Intracellular) - Anti-Integrin alpha 2 antibody [EPR5788] (ab133557)
Intracellular Flow Cytometry analysis of A549 cells labelling Integrin alpha 2 with purified ab133557 at 1/60 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
Flow Cytometry (Intracellular) - Anti-Integrin alpha 2 antibody [EPR5788] (ab133557)
Overlay histogram showing A431 cells stained with unpurified ab133557 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab133557, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was a goat anti-rabbit Alexa Fluorr^® 488 (IgG; H&L) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
OI-RD Scanning - Anti-Integrin alpha 2 antibody [EPR5788] (ab133557)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.