AB10800

Anti-Integrin alpha 2 antibody [HAS-3]

Name: Anti-Integrin alpha 2 antibody [HAS-3] (ab10800) | Abcam
Brand: Abcam Limited
SKU: AB10800
Rating: 3 (4 reviews)

(4 Reviews)

(7 Publications)

Mouse Monoclonal Integrin alpha 2 antibody. Suitable for ICC/IF, Flow Cyt, IHC-P, IHC-Fr, IP and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Cell preparation containing ITGA2 protein.

View Alternative Names

CD49b, CD49B, ITGA2, Integrin alpha-2, CD49 antigen-like family member B, Collagen receptor, Platelet membrane glycoprotein Ia, VLA-2 subunit alpha, GPIa

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

HAS-3

Isotype

IgG2a

Carrier free

Reacts with

Human

Applications

IHC-P, ICC/IF, Flow Cyt, IHC-Fr, IP

applications

Immunogen

Cell preparation containing ITGA2 protein. The exact immunogen used to generate this antibody is proprietary information.

P17301

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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Product details

The antibody co-precipitates alpha 2 beta 1 integrin from keratinocytes and inhibits HGF induced branching morphogenesis and motility of human mammary epithelial HB2 cells in collagen.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Integrin alpha 2 also known as CD49b ITGA2 and A2 is a protein that functions as part of the integrin family. Integrins are transmembrane receptors that facilitate cell-extracellular matrix adhesion. Integrin alpha 2 has a molecular mass of approximately 130 kDa and is widely expressed in epithelial cells fibroblasts and platelets. This protein is important for mediating adhesion between cells and the extracellular matrix affecting cell signaling and behavior. Through this adhesion cells can communicate with their environment adjusting their functions accordingly.

Biological function summary

Integrin alpha 2 plays a significant role in cellular processes like migration and proliferation. It forms a heterodimer complex by pairing with the beta 1-integrin subunit contributing to its adhesive and signaling functions. This association enables the integrin alpha 2 complex to bind specific extracellular matrix components such as collagen and laminin. Through these interactions it regulates processes like wound healing and tissue remodeling and affects how cells respond to mechanical stress.

Pathways

Integrin alpha 2 is involved in major signaling routes that influence cell fate and movement. Notably it participates in the PI3K/AKT and MAPK pathways which are important for regulating cell growth and survival. It interacts with signaling proteins like focal adhesion kinase (FAK) and Src family kinases linking extracellular matrix changes to intracellular responses. These pathways help in transmitting signals that coordinate the cell's structural and functional adjustments in response to its surroundings.

Integrin alpha 2 has connections to cancer and fibrosis. In cancers its altered expression may contribute to tumor progression and metastasis often involving interactions with other integrins and matrix metalloproteinases that degrade extracellular components. In fibrosis excessive integrin alpha 2 activity can lead to abnormal tissue remodeling and scarring implicating its role alongside collagen-producing cells. Understanding these connections is vital for developing therapies targeting integrin-mediated pathways in these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Integrin alpha-2/beta-1 is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix.. (Microbial infection) Integrin ITGA2 : ITGB1 acts as a receptor for Human rotavirus A.. (Microbial infection) Integrin ITGA2 : ITGB1 acts as a receptor for Human echoviruses 1 and 8.

See full target information ITGA2

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

International journal of ophthalmology 13:1202-1209 PubMed32821673

2020

Regulation of opticin on bioactivity of retinal vascular endothelial cells cultured in collagen.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Xing,Xue-Ke Li,Si-Duo Lu,Jin Ma

Matrix biology : journal of the International Society for Matrix Biology 85-86:112-127 PubMed31189077

2019

Transcriptomic analysis reveals that BMP4 sensitizes glioblastoma tumor-initiating cells to mechanical cues.

Applications

Unspecified application

Species

Unspecified reactive species

Jasmine H Hughes,Jeanette M Ewy,Joseph Chen,Sophie Y Wong,Kevin M Tharp,Andreas Stahl,Sanjay Kumar

PloS one 6:e26753 PubMed22110593

2011

Activation of c-MET induces a stem-like phenotype in human prostate cancer.

Applications

IHC-Fr

Species

Human

Geert J L H van Leenders,Rajesh Sookhlall,Wilma J Teubel,Corrina M A de Ridder,Suzanne Reneman,Andrea Sacchetti,Kees J Vissers,Wytske van Weerden,Guido Jenster

American journal of physiology. Lung cellular and molecular physiology 299:L169-83 PubMed20435685

2010

Can lineage-specific markers be identified to characterize mesenchyme-derived cell populations in the human airways?

Applications

ICC/IF

Species

Human

Shailendra R Singh,Charlotte K Billington,Ian Sayers,Ian P Hall

Journal of cell science 111 ( Pt 4):521-32 PubMed9443900

1998

Integrin-matrix interactions affect the form of the structures developing from human mammary epithelial cells in collagen or fibrin gels.

Applications

Unspecified application

Species

Unspecified reactive species

D Alford,D Baeckström,M Geyp,P Pitha,J Taylor-Papadimitriou

Carcinogenesis 14:2171-6 PubMed7693359

1993

Comparison of integrin expression and terminal differentiation capacity in cell lines derived from oral squamous cell carcinomas.

Applications

Unspecified application

Species

Unspecified reactive species

M Sugiyama,P M Speight,S S Prime,F M Watt

Cell adhesion and communication 1:55-66 PubMed7521749

1993

Evidence against a major role for integrins in calcium-dependent intercellular adhesion of epidermal keratinocytes.

Applications

Unspecified application

Species

Unspecified reactive species

M L Tenchini,J C Adams,C Gilberty,J Steel,D L Hudson,M Malcovati,F M Watt

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Integrin alpha 2 antibody [HAS-3]

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise