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AB11027

Anti-Integrin alpha 2b + beta 3 antibody [P256]

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(4 Publications)

Mouse Monoclonal CD41 antibody. Suitable for IP, WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Cell preparation containing ITGB3 protein.

View Alternative Names

CD41, GP2B, ITGAB, ITGA2B, Integrin alpha-IIb, GPalpha IIb, Platelet membrane glycoprotein IIb, GPIIb

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

P256

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IP, WB

applications

Immunogen

Cell preparation containing ITGB3 protein. The exact immunogen used to generate this antibody is proprietary information.

P05106

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Integrin alpha 2b + beta 3 also known as glycoprotein IIb/IIIa is an important heterodimeric integrin protein complex essential for platelet adhesion and aggregation. This integrin with a mass around 210 kDa functions as a receptor for fibrinogen and other adhesive proteins. Expression of integrin alpha 2b beta 3 is found mainly in platelets and megakaryocytes. The integrin protein's alpha 2b subunit contributes to its specificity and is involved in binding to the ligand while the beta 3 subunit influences activation and signal transduction.
Biological function summary

Integrin alpha 2b beta 3 plays a major role in hemostasis by mediating platelet cohesion and clot formation at sites of vascular injury. This integrin is an important part of the platelet glycoprotein complex that forms a bridge to link platelets through fibrinogen. Through its function integrin alpha 2b beta 3 is responsible for ensuring the stability of clots in blood vessels preventing loss of blood. It also interacts with various signaling molecules inside platelets facilitating processes like shape change and granule secretion.

Pathways

Integrin alpha 2b beta 3 involvement in hemostatic pathways reveals its importance in thrombocytopenic mechanisms. Inside the platelet signaling pathways such as phosphatidylinositol 3-kinase (PI3K) and Src family kinases become activated upon ligand binding to this integrin. These pathways involve other proteins like talin and kindlin which play roles in integrin inside-out activation. Additionally crosstalk with other integrins reinforces platelet adhesion and spreading.

Integrin alpha 2b beta 3 associations with thrombotic disorders highlight its clinical significance. Its dysregulation can lead to conditions such as myocardial infarction and stroke where excessive platelet aggregation causes vascular blockages. The integrin also connects to disorders like Glanzmann's thrombasthenia a condition resulting from mutations in the integrin alpha 2b or beta 3 subunits leading to impaired clot formation. The integrin protein's interaction with fibrinogen and von Willebrand factor further emphasizes its impact on thrombotic diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. It recognizes the sequence R-G-D in a wide array of ligands. It recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain (By similarity). Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen (PubMed : 9111081). This step leads to rapid platelet aggregation which physically plugs ruptured endothelial cell surface (By similarity).
See full target information ITGA2B

Additional targets

ITGB3
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cell adhesion & migration 17:1-14 PubMed36944577

2023

Inhibition of Integrin αβ-FAK-MAPK signaling constrains the invasion of T-ALL cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lan Huang,Yao Zhu,Qinglin Kong,Xianmin Guan,Xiaoying Lei,Luying Zhang,Hui Yang,Xinyuan Yao,Shaoyan Liang,Xizhou An,Jie Yu

European journal of biochemistry 190:177-83 PubMed2364945

1990

Activation of platelets induced by mAb P256 specific for glycoprotein IIb-IIIa. Possible evidence for a role for IIb-IIIa in membrane signal transduction.

Applications

Unspecified application

Species

Unspecified reactive species

C Bachelot,F Rendu,C Boucheix,N Hogg,S Levy-Toledano

Blood 64:139-46 PubMed6234033

1984

Monoclonal antibodies specific for platelet glycoproteins react with human monocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Y Bai,H Durbin,N Hogg

European journal of immunology 13:521-7 PubMed6603362

1983

Two monoclonal antibodies identifying a subset of human peripheral mononuclear cells with natural killer cell activity.

Applications

Unspecified application

Species

Unspecified reactive species

Y Bai,P C Beverley,R W Knowles,W F Bodmer
View all publications
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