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Mouse Monoclonal Integrin alpha 2 antibody. Suitable for Flow Cyt, ICC/IF and reacts with Human samples. Cited in 14 publications.


Images

Flow Cytometry - Anti-Integrin alpha 2+beta 1 antibody [16B4] (AB30483), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha 2+beta 1 antibody [16B4] (AB30483), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha 2+beta 1 antibody [16B4] (AB30483), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytICC/IF
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
0.1 µg for 106 Cells
Notes

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Tested
Tested

Species
Human
Dilution info
5.00000-10.00000 µg/mL
Notes

-

Associated Products

Select an associated product type

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Target data

Function

Integrin alpha-2/beta-1 is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix. (Microbial infection) Integrin ITGA2:ITGB1 acts as a receptor for Human rotavirus A. (Microbial infection) Integrin ITGA2:ITGB1 acts as a receptor for Human echoviruses 1 and 8.

Additional Targets

ITGB1

Alternative names

CD49b, CD49B, ITGA2, Integrin alpha-2, CD49 antigen-like family member B, Collagen receptor, Platelet membrane glycoprotein Ia, VLA-2 subunit alpha, GPIa

Recommended products

Mouse Monoclonal Integrin alpha 2 antibody. Suitable for Flow Cyt, ICC/IF and reacts with Human samples. Cited in 14 publications.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
16B4
Purification technique
Affinity purification
Concentration
Loading...
Purification notes

ab30483 was purified from tissue culture supernatant

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot

Notes

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Integrin alpha 2+beta 1 also known as integrin a2b1 or alpha 2 beta 1 integrin is a heterodimeric cell surface receptor with a mass of approximately 150 kDa. It is expressed on a variety of cells including epithelial and endothelial cells as well as platelets. The primary role of integrin alpha 2+beta 1 involves mediating cell adhesion to collagen a major component of the extracellular matrix. This interaction is critical for maintaining tissue architecture and integrity. The receptor's structure allows it to bind to specific ligands facilitating cell communication and signal transduction across the cell membrane.

Biological function summary

Integrin alpha 2+beta 1 contributes to processes such as cell migration proliferation and differentiation. This integrin functions as part of a larger integrin family complex which includes related integrins like alpha 1 beta 1 and alpha 6 beta 4. These integrins share similarities in structure and function but have distinct binding specificities and tissue distributions. The presence of integrin alpha 2+beta 1 in various tissues indicates its role in tissue-specific cellular activities underlying its contribution to diverse biological functions.

Pathways

Integrin alpha 2+beta 1 is involved in the MAPK/ERK and PI3K/AKT signaling pathways. These pathways are essential for transmitting signals from the extracellular environment to the cell nucleus influencing gene expression and cellular responses. The integrin interacts with proteins such as focal adhesion kinase (FAK) and Src family kinases which are important for initiating downstream signaling events. The interaction of integrin alpha 2+beta 1 within these pathways highlights its role in regulating cellular responses to environmental changes and stresses.

Associated diseases and disorders

Integrin alpha 2+beta 1 has been linked to cancer metastasis and fibrosis. Its role in promoting cell adhesion and migration is critical in the progression of these diseases. In cancer integrin alpha 2+beta 1 supports tumor cell invasion through the basement membrane by interacting with proteins like matrix metalloproteinases. Fibrosis involves excessive tissue scarring where the integrin contributes to the proliferation of myofibroblasts and deposition of extracellular matrix. Understanding the involvement of integrin alpha 2+beta 1 in these conditions could lead to potential therapeutic targets for intervention.

Product promise

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3 product images

  • Flow Cytometry - Anti-Integrin alpha 2+beta 1 antibody [16B4] (ab30483), expandable thumbnail

    Flow Cytometry - Anti-Integrin alpha 2+beta 1 antibody [16B4] (ab30483)

    Overlay histogram showing HT1080 cells stained with ab30483 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab30483, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was a goat anti-mouse Alexa Fluor® 488 (IgG; H+L) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HT1080 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha 2+beta 1 antibody [16B4] (ab30483), expandable thumbnail
    This image is courtesy of a customer review submitted by Dr Sharon Sneddon

    Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha 2+beta 1 antibody [16B4] (ab30483)

    ab30483 staining human breast cancer cells by ICC/IF. Cells were PFA fixed and blocked with 1% serum for 16 hours at 20°C prior to incubating with ab30483 (at 30μg/ml) for 16 hours at 4°C. An Alexa Fluor® 488 conjugated goat anti-mouse antibody, diluted 1/200, was used as the secondary.

  • Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha 2+beta 1 antibody [16B4] (ab30483), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha 2+beta 1 antibody [16B4] (ab30483)

    ab30483 stained in A431cells. The cells were fixed with 4% paraformaldehyde (10min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 1 hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with ab30483 at 5 μg/ml and Anti-beta Tubulin antibody - Loading Control ab6046 (Rabbit polyclonal to beta tubulin) at 1 ug/ml overnight at +4°C. The secondary antibodies were Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 (colored green) used at 1 ug/ml and Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed ab150087 (pseudo-colored red) used at 2 μg/ml for 1 hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43 μM for 1 hour at room temperature.

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