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AB24697

Anti-Integrin alpha 2+beta 1 antibody [P1E6]

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(22 Publications)

Mouse Monoclonal Integrin alpha 2 antibody. Suitable for Flow Cyt (Intra), ICC/IF and reacts with Human samples. Cited in 22 publications.

View Alternative Names

CD49b, CD49B, ITGA2, Integrin alpha-2, CD49 antigen-like family member B, Collagen receptor, Platelet membrane glycoprotein Ia, VLA-2 subunit alpha, GPIa, CD29, FNRB, MDF2, MSK12, ITGB1, Integrin beta-1, Fibronectin receptor subunit beta, Glycoprotein IIa, VLA-4 subunit beta, GPIIA

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha 2+beta 1 antibody [P1E6] (AB24697)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha 2+beta 1 antibody [P1E6] (AB24697)

ab24697 staining Intergri alpha 2 + beta 1 in HepG2 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab24697 at 5µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

Also suitable in cells fixed with 100% methanol (5 min).

Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.

Flow Cytometry (Intracellular) - Anti-Integrin alpha 2+beta 1 antibody [P1E6] (AB24697)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Integrin alpha 2+beta 1 antibody [P1E6] (AB24697)

Overlay histogram showing HT1080 cells stained with ab24697 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab24697, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was a Goat Anti-Mouse DyLight® 488 (IgG; H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 80% methanol (5 min) fixed HT1080 cells used under the same conditions.

Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

P1E6

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Alternative Products

Primary Antibodies

AB30483

Anti-Integrin alpha 2+beta 1 antibody [16B4]

Trial Size

Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha 2+beta 1 antibody [16B4] (AB30483)

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Primary Antibodies

AB230290

Anti-Integrin alpha 2+beta 1 antibody [16B4] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha 2+beta 1 antibody [16B4] - BSA and Azide free (AB230290)

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Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB150361

Anti-Integrin alpha 5 antibody [EPR7854]

RabMAb|Recombinant|KO Validated|20ul selling size

Western blot - Anti-Integrin alpha 5 antibody [EPR7854] (AB150361)

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Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

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Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1 µg for 10^6 Cells", "FlowCytIntra-species-notes": "<p>(Also PMID - 19780039)</p><p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5-10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Product details

In imunofluorescence, ab24697 can be used to detect a2 in collagen dependent focal adhesions.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 6.97% L-Arginine
Shipped at conditions
Blue Ice
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Integrin alpha 2+beta 1 also known as integrin a2b1 or alpha 2 beta 1 integrin is a heterodimeric cell surface receptor with a mass of approximately 150 kDa. It is expressed on a variety of cells including epithelial and endothelial cells as well as platelets. The primary role of integrin alpha 2+beta 1 involves mediating cell adhesion to collagen a major component of the extracellular matrix. This interaction is critical for maintaining tissue architecture and integrity. The receptor's structure allows it to bind to specific ligands facilitating cell communication and signal transduction across the cell membrane.
Biological function summary

Integrin alpha 2+beta 1 contributes to processes such as cell migration proliferation and differentiation. This integrin functions as part of a larger integrin family complex which includes related integrins like alpha 1 beta 1 and alpha 6 beta 4. These integrins share similarities in structure and function but have distinct binding specificities and tissue distributions. The presence of integrin alpha 2+beta 1 in various tissues indicates its role in tissue-specific cellular activities underlying its contribution to diverse biological functions.

Pathways

Integrin alpha 2+beta 1 is involved in the MAPK/ERK and PI3K/AKT signaling pathways. These pathways are essential for transmitting signals from the extracellular environment to the cell nucleus influencing gene expression and cellular responses. The integrin interacts with proteins such as focal adhesion kinase (FAK) and Src family kinases which are important for initiating downstream signaling events. The interaction of integrin alpha 2+beta 1 within these pathways highlights its role in regulating cellular responses to environmental changes and stresses.

Integrin alpha 2+beta 1 has been linked to cancer metastasis and fibrosis. Its role in promoting cell adhesion and migration is critical in the progression of these diseases. In cancer integrin alpha 2+beta 1 supports tumor cell invasion through the basement membrane by interacting with proteins like matrix metalloproteinases. Fibrosis involves excessive tissue scarring where the integrin contributes to the proliferation of myofibroblasts and deposition of extracellular matrix. Understanding the involvement of integrin alpha 2+beta 1 in these conditions could lead to potential therapeutic targets for intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Integrin alpha-2/beta-1 is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix.. (Microbial infection) Integrin ITGA2 : ITGB1 acts as a receptor for Human rotavirus A.. (Microbial infection) Integrin ITGA2 : ITGB1 acts as a receptor for Human echoviruses 1 and 8.
See full target information ITGA2

Additional targets

ITGB1
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Publications (22)

Recent publications for all applications. Explore the full list and refine your search

EMBO reports 24:e56850 PubMed37846507

2023

An ULK1/2-PXN mechanotransduction pathway suppresses breast cancer cell migration.

Applications

Unspecified application

Species

Unspecified reactive species

Peigang Liang,Jiaqi Zhang,Yuchen Wu,Shanyuan Zheng,Zhaopeng Xu,Shuo Yang,Jinfang Wang,Suibin Ma,Li Xiao,Tianhui Hu,Wenxue Jiang,Chaoqun Huang,Qiong Xing,Mondira Kundu,Bo Wang

Bioengineering & translational medicine 8:e10324 PubMed36684084

2023

3D bioprinting of an implantable xeno-free vascularized human skin graft.

Applications

Unspecified application

Species

Unspecified reactive species

Tania Baltazar,Bo Jiang,Alejandra Moncayo,Jonathan Merola,Mohammad Z Albanna,W Mark Saltzman,Jordan S Pober

Advanced healthcare materials 11:e2102337 PubMed34968011

2022

Engineered Cell-Secreted Extracellular Matrix Modulates Cell Spheroid Mechanosensing and Amplifies Their Response to Inductive Cues for the Formation of Mineralized Tissues.

Applications

Unspecified application

Species

Unspecified reactive species

Tomas Gonzalez-Fernandez,Alejandro J Tenorio,Augustine M Saiz,J Kent Leach

Blood 138:1359-1372 PubMed34375384

2021

An alternate covalent form of platelet αIIbβ3 integrin that resides in focal adhesions and has altered function.

Applications

Unspecified application

Species

Unspecified reactive species

Aster E Pijning,Mitchell T Blyth,Michelle L Coote,Freda Passam,Joyce Chiu,Philip J Hogg

International journal of molecular sciences 22: PubMed33807408

2021

Collagen I Modifies Connexin-43 Hemichannel Activity via Integrin α2β1 Binding in TGFβ1-Evoked Renal Tubular Epithelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Joe A Potter,Gareth W Price,Chelsy L Cliff,Colin R Green,Paul E Squires,Claire E Hills

Bioactive materials 6:3541-3556 PubMed33842740

2021

Decellularized Disc Hydrogels for hBMSCs tissue-specific differentiation and tissue regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Yizhong Peng,Xiangcheng Qing,Hui Lin,Donghua Huang,Jinye Li,Shuo Tian,Sheng Liu,Xiao Lv,Kaige Ma,Rui Li,Zilong Rao,Ying Bai,Songfeng Chen,Ming Lei,Daping Quan,Zengwu Shao

Medicine 99:e20628 PubMed32569193

2020

Different effects of total flavonoids from Arachniodes exilis on human umbilical cord mesenchymal stem cells in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Wenmin Yu,Wenlong Hu,Xiumei Ke,Xufeng Zhou,Changchang Yin,Ming Yin

Marine drugs 17: PubMed31771240

2019

Increased Cell Detachment Ratio of Mesenchymal-Type Lung Cancer Cells on pH-Responsive Chitosan through the β3 Integrin.

Applications

Unspecified application

Species

Unspecified reactive species

Chia-Hsiang Yen,Tai-Horng Young,Meng-Chi Hsieh,Li-Jen Liao,Tsung-Wei Huang

Acta biomaterialia 96:310-320 PubMed31255664

2019

Extracellular matrix type modulates mechanotransduction of stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Alice E Stanton,Xinming Tong,Fan Yang

Theranostics 7:4013-4028 PubMed29109795

2017

The Use of PET Imaging for Prognostic Integrin αβ Phenotyping to Detect Non-Small Cell Lung Cancer and Monitor Drug Resistance Responses.

Applications

Unspecified application

Species

Unspecified reactive species

Chiun-Wei Huang,Wen-Chuan Hsieh,Shih-Ting Hsu,Yi-Wen Lin,Yi-Hsiu Chung,Wen-Chi Chang,Han Chiu,Yun Han Lin,Chung-Pu Wu,Tzu-Chen Yen,Feng-Ting Huang
View all publications
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Product promise

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