Anti-Integrin alpha 3 antibody

• WB

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Western blot - Anti-Integrin alpha 3 antibody (AB190731)

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Western blot - Anti-Integrin alpha 3 antibody (ab190731)

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HepG2 cell extract

Predicted band size: 119 kDa

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• WB

CiteAb

Western blot - Anti-Integrin alpha 3 antibody (AB190731)

Integrin alpha 3 western blot using anti-Integrin alpha 3 antibody ab190731. Publication image and figure legend from Temoche-Diaz, M. M., Shurtleff, M. J., et al., 2019, Elife, PubMed 31436530.

ab190731 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab190731 please see the product overview.

Two biochemically distinct sEV sub-populations are released by MDA-MB-231 cells.(a) Schematic showing the two-step purification methodology. Differential ultracentrifugation was followed by buoyant density flotation in a linear iodixanol gradient (Figure 1—figure supplement 1). (b) Immunoblot across the iodixanol gradient for classical sEV markers as well as other non-vesicular RNP components. The two discrete sub-populations are indicated. CD63, a glycosylated protein, migrates heterogeneously. (c) Quantification of individual proteins as in 1b. The black line delineates the maximum signal across the gradient showing three distinctive areas. The first, second and third peak represent the vLD, vHD and non-vesicular RNP fractions. (d) Nanoparticle tracking analysis showing the size distribution of the vHD and vLD sub-populations. The high-speed pellet is also shown. (d) Quantification of the particle number per sEV sub-population using Nanoparticle tracking analysis. Data plotted are from two independent experiments; error bars represent standard deviation from independent samples. (e) Bioanalyzer analysis of the vHD and vLD RNA. The high-speed pellet RNA is also shown.Linearity of iodixanol density gradient.Calculated iodixanol fraction densities collected from the top to bottom of the gradient (fractions 1 to 25) as measured by refractometry (Figure 1—figure supplement 1—source data 1). Fractions were collected post 160,000 g ultracentrifugation (Figure 1a). In red, fractions corresponding to the vLD sub-population. In blue, fractions corresponding to the vHD sub-population.10.7554/eLife.47544.004Figure 1—figure supplement 1—source data 1.Refraction index and calculated densities of the fractions across the gradient (31 fractions in total).Refraction index and calculated densities of the fractions across the gradient (31 fractions in total).

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