AB242196

Anti-Integrin alpha 3 antibody [CL6937]

Name: Anti-Integrin alpha 3 antibody [CL6937] (ab242196) | Abcam
Brand: Abcam Limited
SKU: AB242196
Price: 570 USD
Availability: InStock

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(6 Publications)

Mouse Monoclonal Integrin alpha 3 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ITGA3 aa 600-750.

View Alternative Names

CD49c, MSK18, ITGA3, Integrin alpha-3, CD49 antigen-like family member C, FRP-2, Galactoprotein B3, VLA-3 subunit alpha, GAPB3

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 3 antibody [CL6937] (AB242196)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 3 antibody [CL6937] (AB242196)

Paraffin-embedded human lung tissue stained for Integrin alpha 3 using ab242196 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 3 antibody [CL6937] (AB242196)

Paraffin-embedded human colon tissue stained for Integrin alpha 3 using ab242196 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 3 antibody [CL6937] (AB242196)

Paraffin-embedded human placenta tissue stained for Integrin alpha 3 using ab242196 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 3 antibody [CL6937] (AB242196)

Negative control :

Paraffin-embedded human liver tissue stained for Integrin alpha 3 using ab242196 at 1/100 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-Integrin alpha 3 antibody [CL6937] (AB242196)

Remaining relative intensity is presented.

Loading control : Anti-GAPDH

All lanes:

Western blot - Anti-Integrin alpha 3 antibody [CL6937] (ab242196) at 1 µg/mL

Lane 1:

U-87 MG (human glioblastoma-astrocytoma epithelial cell line) cells transfected with control siRNA

Lane 2:

U-87 MG cells transfected with target specific siRNA probe #1

Lane 3:

U-87 MG cells transfected with target specific siRNA probe #2

Predicted band size: 119 kDa

false

Supplier Data

Western blot - Anti-Integrin alpha 3 antibody [CL6937] (AB242196)

All lanes:

Western blot - Anti-Integrin alpha 3 antibody [CL6937] (ab242196) at 1 µg/mL

All lanes:

U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate

Predicted band size: 119 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL6937

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human ITGA3 aa 600-750. The exact immunogen used to generate this antibody is proprietary information.

P26006

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Purified from TCS.

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Integrin alpha 3 also known as ITGA3 or alpha 3 is a protein that plays an important role in cell adhesion and signaling. It forms complexes with other integrins to mediate interactions between cells and the extracellular matrix. Integrin alpha 3 is a type I transmembrane protein with a molecular weight of approximately 120 kDa. This protein is primarily expressed in epithelial tissues including the skin kidneys and respiratory tract.

Biological function summary

Integrin alpha 3 functions as a cell surface receptor that helps regulate cell migration proliferation and differentiation. It is a part of the alpha-3-beta-1 integrin complex which interacts with extracellular proteins like laminin-5 and collagen. This interaction facilitates the transmission of signals from the outside to the inside of the cell affecting various cellular responses and activities.

Pathways

Integrin alpha 3 plays a significant role in the cell signaling and communication pathways. It is involved in the pathways like focal adhesion and MAPK signaling. In these pathways integrin alpha 3 interacts with other proteins such as paxillin and FAK (focal adhesion kinase) which are critical for the downstream signaling that regulates cell shape and movement.

Integrin alpha 3 is linked to conditions like skin blistering diseases and cancers particularly those affecting the epithelial tissues. Alterations in integrin alpha 3 expression or function can disturb cell adhesion leading to diseases like epidermolysis bullosa. Additionally in cancer integrin alpha 3 often collaborates with proteins like MMP-9 to promote tumor invasion and metastasis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Integrin alpha-3/beta-1 is a receptor for fibronectin, laminin, collagen, epiligrin, thrombospondin and CSPG4. Integrin alpha-3/beta-1 provides a docking site for FAP (seprase) at invadopodia plasma membranes in a collagen-dependent manner and hence may participate in the adhesion, formation of invadopodia and matrix degradation processes, promoting cell invasion. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration.. (Microbial infection) Integrin ITGA3 : ITGB1 may act as a receptor for R.delemar CotH7 in alveolar epithelial cells, which may be an early step in pulmonary mucormycosis disease progression.

See full target information ITGA3

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Breast cancer research : BCR 26:187 PubMed39707454

2024

Cilengitide sensitivity is predicted by overall integrin expression in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Nomeda Girnius,Aylin Z Henstridge,Benjamin Marks,Jeffrey K Yu,G Kenneth Gray,Chris Sander,Ioannis K Zervantonakis,Augustin Luna

Cancer research 83:195-218 PubMed36409826

2022

Aberrant L-Fucose Accumulation and Increased Core Fucosylation Are Metabolic Liabilities in Mesenchymal Glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Valentina Pieri,Alberto L Gallotti,Denise Drago,Manuela Cominelli,Ilaria Pagano,Valentina Conti,Silvia Valtorta,Angela Coliva,Sara Lago,Daniela Michelatti,Luca Massimino,Federica Ungaro,Laura Perani,Antonello Spinelli,Antonella Castellano,Andrea Falini,Alessio Zippo,Pietro L Poliani,Rosa Maria Moresco,Annapaola Andolfo,Rossella Galli

Cell death & disease 13:486 PubMed35597804

2022

Integrin α3/α6 and αV are implicated in ADAM15-activated FAK and EGFR signalling pathway individually and promote non-small-cell lung cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Jieqi Zhou,Anqi Wang,Tingting Cai,Yue Li,Wenwen Du,Yang Zhang,Ruochen Zhang,Weijie Zhang,Jianjie Zhu,Yuanyuan Zeng,Jian-An Huang,Zeyi Liu

Frontiers in cell and developmental biology 9:678670 PubMed34504839

2021

Cancer-Associated Fibroblasts Promote Vascular Invasion of Hepatocellular Carcinoma via Downregulating Decorin-integrin β1 Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaobo Zheng,Peng Wang,Li Li,Jing Yu,Chune Yu,Liangliang Xu,Lian Li,Fuzhen Dai,Lei Feng,Hong Zou,Xiaobo Chen,Ming Zhang,Mingqing Xu

Development (Cambridge, England) 148: PubMed34184026

2021

scRNA sequencing uncovers a TCF4-dependent transcription factor network regulating commissure development in mouse.

Applications

Unspecified application

Species

Unspecified reactive species

Marie-Theres Wittmann,Sayako Katada,Elisabeth Sock,Philipp Kirchner,Arif B Ekici,Michael Wegner,Kinichi Nakashima,Dieter Chichung Lie,André Reis

Journal of experimental & clinical cancer research : CR 40:192 PubMed34108040

2021

CD151 drives cancer progression depending on integrin α3β1 through EGFR signaling in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jianjie Zhu,Tingting Cai,Jieqi Zhou,Wenwen Du,Yuanyuan Zeng,Ting Liu,Yulong Fu,Yue Li,Qian Qian,Xiuwei H Yang,Qinglin Li,Jian-An Huang,Zeyi Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Integrin alpha 3 antibody [CL6937]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 3 antibody [CL6937] (AB242196)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 3 antibody [CL6937] (AB242196)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 3 antibody [CL6937] (AB242196)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 3 antibody [CL6937] (AB242196)

Western blot - Anti-Integrin alpha 3 antibody [CL6937] (AB242196)

Western blot - Anti-Integrin alpha 3 antibody [CL6937] (AB242196)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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