AB202969

Anti-Integrin alpha 4/CD49D antibody

Name: Anti-Integrin alpha 4/CD49D antibody (ab202969) | Abcam
Brand: Abcam Limited
SKU: AB202969
Price: 510 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(10 Publications)

Rabbit Polyclonal Integrin alpha 4/CD49D antibody. Suitable for Flow Cyt, IHC-P and reacts with Human, Mouse samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Human ITGA4 aa 550-650 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

CD49d, CD49D, ITGA4, Integrin alpha-4, CD49 antigen-like family member D, Integrin alpha-IV, VLA-4 subunit alpha

2 Images

Flow Cytometry - Anti-Integrin alpha 4/CD49D antibody (AB202969)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-Integrin alpha 4/CD49D antibody (AB202969)

Flow Cytometry analysis of Human U937 cells labeling Integrin alpha 4/CD49D with ab202969 at 0.2 μg for 30 minutes followed by incubation with a PE Conjugated secondary (green) for 30 minutes. Blue - control cells, light blue - secondary only, orange - isotype control.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 4/CD49D antibody (AB202969)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 4/CD49D antibody (AB202969)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Mouse lung labeling CD49d with ab202969 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, Flow Cyt

applications

Immunogen

Synthetic Peptide within Human ITGA4 aa 550-650 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P13612

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/20 - 1/100", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p>Use at 1/50 - 1/200 with fluorescent detection methods.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Integrin alpha 4 also known as CD49D or ITGA4 is a transmembrane receptor with a mass of approximately 150 kDa. This protein is expressed on the surface of leukocytes including T and B lymphocytes monocytes and eosinophils. Integrin alpha 4 functions by interacting mechanically with the extracellular matrix components and endothelial cell counter-receptors like VCAM-1 facilitating cell adhesion and migration. Its expression is vital for the immune cells' ability to move and locate sites of inflammation or injury.

Biological function summary

Integrin alpha 4 forms a heterodimer complex with beta subunits like beta 1 (forming VLA-4) or beta 7 allowing it to mediate adhesion and signaling pathways. In particular the alpha 4 and beta 1 dimerization gives rise to a high-affinity receptor for fibronectin and VCAM-1. This interaction is essential for regulating immune responses and maintaining cell positioning within tissues. The presence of this complex on leukocytes drives their ability to navigate through the vasculature to inflamed areas.

Pathways

Integrin alpha 4 participates in significant signal transduction pathways including the MAPK and PI3K-Akt pathways. These pathways modulate cell survival proliferation and migration. In the MAPK pathway integrin alpha 4 influences cell signaling cascades by interacting with other proteins like TALIN or PAXILLIN which are important for integrin-mediated signal relay. In connection with PI3K-Akt pathway alpha 4 integrins work closely with proteins in regulating cellular adhesion and motility showing a dynamic interplay in immune regulation.

Integrin alpha 4 plays an important role in various immune-related diseases such as multiple sclerosis and Crohn's disease. The protein's participation in leukocyte adhesion and trafficking makes it a target for therapeutic intervention. Natalizumab a monoclonal antibody against alpha 4 integrin showcases the critical link between the protein and disease treatment by blocking leukocyte migration to reduce inflammation. Additionally integrin alpha 4's interaction with proteins like VCAM-1 and VLA-4 highlights its relevance in these autoimmune conditions highlighting its importance in disease pathophysiology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells. ITGA4 : ITGB1 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling (PubMed : 23125415). ITGA4 : ITGB1 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed : 18635536, PubMed : 25398877). Integrin ITGA4 : ITGB1 represses PRKCA-mediated L-type voltage-gated channel Ca(2+) influx and ROCK-mediated calcium sensitivity in vascular smooth muscle cells via its interaction with SVEP1, thereby inhibiting vasocontraction (PubMed : 35802072).

See full target information ITGA4

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 13:5700 PubMed36171212

2022

In vivo self-assembled siRNA as a modality for combination therapy of ulcerative colitis.

Applications

Unspecified application

Species

Unspecified reactive species

Xinyan Zhou,Mengchao Yu,Luzhen Ma,Jinyu Fu,Jingwei Guo,Jieqiong Lei,Zheng Fu,Yong Fu,Qipeng Zhang,Chen-Yu Zhang,Xi Chen

Bioactive materials 20:179-193 PubMed35663336

2022

A bioactive material with dual integrin-targeting ligands regulates specific endogenous cell adhesion and promotes vascularized bone regeneration in adult and fetal bone defects.

Applications

Unspecified application

Species

Unspecified reactive species

Dake Hao,Ruiwu Liu,Tomas Gonzalez Fernandez,Christopher Pivetti,Jordan Elizabeth Jackson,Edwin Samuel Kulubya,Hong-Jiang Jiang,Hai-Yang Ju,Wen-Liang Liu,Alyssa Panitch,Kit S Lam,J Kent Leach,Diana L Farmer,Aijun Wang

International journal of molecular sciences 22: PubMed34576061

2021

Nintedanib Regulates GRK2 and CXCR2 to Reduce Neutrophil Recruitment in Endotoxin-Induced Lung Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Vincent Yi-Fong Su,Wei-Chih Chen,Wen-Kuang Yu,Huai-Hsuan Wu,Hao Chen,Kuang-Yao Yang

International journal of molecular sciences 22: PubMed34074039

2021

Induced Pluripotent Stem Cell-Derived Conditioned Medium Promotes Endogenous Leukemia Inhibitory Factor to Attenuate Endotoxin-Induced Acute Lung Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Vincent Yi-Fong Su,Shih-Hwa Chiou,Wei-Chih Chen,Wen-Kuang Yu,Huai-Hsuan Wu,Hao Chen,Kuang-Yao Yang

Acta neuropathologica communications 9:67 PubMed33853689

2021

TNFα secreted by glioma associated macrophages promotes endothelial activation and resistance against anti-angiogenic therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Qingxia Wei,Olivia Singh,Can Ekinci,Jaspreet Gill,Mira Li,Yasin Mamatjan,Shirin Karimi,Severa Bunda,Sheila Mansouri,Kenneth Aldape,Gelareh Zadeh

Frontiers in pharmacology 11:617836 PubMed33584300

2021

Leukocyte Integrin Antagonists as a Novel Option to Treat Dry Age-Related Macular Degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Monica Baiula,Alberto Caligiana,Andrea Bedini,Junwei Zhao,Federica Santino,Martina Cirillo,Luca Gentilucci,Daria Giacomini,Santi Spampinato

Science advances 6: PubMed33127669

2020

Leukemia-on-a-chip: Dissecting the chemoresistance mechanisms in B cell acute lymphoblastic leukemia bone marrow niche.

Applications

Unspecified application

Species

Unspecified reactive species

Chao Ma,Matthew T Witkowski,Jacob Harris,Igor Dolgalev,Sheetal Sreeram,Weiyi Qian,Jie Tong,Xin Chen,Iannis Aifantis,Weiqiang Chen

Scientific reports 10:18560 PubMed33122751

2020

HIFα independent mechanisms in renal carcinoma cells modulate divergent outcomes in fibronectin assembly mediated by hypoxia and CoCl.

Applications

Unspecified application

Species

Unspecified reactive species

Carina Magdaleno,Leah Dixon,Narendiran Rajasekaran,Archana Varadaraj

Journal of toxicologic pathology 33:121-129 PubMed32425345

2020

Hyperglycemia contributes to the development of Leydig cell hyperplasia in male Spontaneously Diabetic Torii rats.

Applications

Unspecified application

Species

Unspecified reactive species

Yoshitomi Nakane,Yusuke Kemmochi,Naoto Ogawa,Tomohiko Sasase,Takeshi Ohta,Yoshikazu Higami,Fumio Fukai

Cellular physiology and biochemistry : internation 46:36-45 PubMed29566360

2018

The Antimuscarinic Agent Tolterodine Regulates Bladder Extracellular Matrix in Partial Bladder Outlet Obstruction in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Tong-Xin Yang,De-Yi Luo,Yi-Fei Lin,Qiang Liu,Xiang Cai,Jian-Zhong Ai,Hong Li,Hong Shen,Kun-Jie Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Integrin alpha 4/CD49D antibody

Flow Cytometry - Anti-Integrin alpha 4/CD49D antibody (AB202969)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 4/CD49D antibody (AB202969)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (10)

Product promise