Rabbit Polyclonal Integrin alpha 4/CD49D antibody. Suitable for Flow Cyt, IHC-P and reacts with Human, Mouse samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human ITGA4 aa 550-650 conjugated to Keyhole Limpet Haemocyanin.
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- Nature communications 13:57002022In vivo self-assembled siRNA as a modality for combination therapy of ulcerative colitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXinyan Zhou,Mengchao Yu,Luzhen Ma,Jinyu Fu,Jingwei Guo,Jieqiong Lei,Zheng Fu,Yong Fu,Qipeng Zhang,Chen-Yu Zhang,Xi ChenPubMed 36171212
- International journal of molecular sciences 22:2021Nintedanib Regulates GRK2 and CXCR2 to Reduce Neutrophil Recruitment in Endotoxin-Induced Lung Injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVincent Yi-Fong Su,Wei-Chih Chen,Wen-Kuang Yu,Huai-Hsuan Wu,Hao Chen,Kuang-Yao YangPubMed 34576061
- International journal of molecular sciences 22:2021Induced Pluripotent Stem Cell-Derived Conditioned Medium Promotes Endogenous Leukemia Inhibitory Factor to Attenuate Endotoxin-Induced Acute Lung Injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVincent Yi-Fong Su,Shih-Hwa Chiou,Wei-Chih Chen,Wen-Kuang Yu,Huai-Hsuan Wu,Hao Chen,Kuang-Yao YangPubMed 34074039
- Acta neuropathologica communications 9:672021TNFα secreted by glioma associated macrophages promotes endothelial activation and resistance against anti-angiogenic therapy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQingxia Wei,Olivia Singh,Can Ekinci,Jaspreet Gill,Mira Li,Yasin Mamatjan,Shirin Karimi,Severa Bunda,Sheila Mansouri,Kenneth Aldape,Gelareh ZadehPubMed 33853689
- Frontiers in pharmacology 11:6178362021Leukocyte Integrin Antagonists as a Novel Option to Treat Dry Age-Related Macular Degeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMonica Baiula,Alberto Caligiana,Andrea Bedini,Junwei Zhao,Federica Santino,Martina Cirillo,Luca Gentilucci,Daria Giacomini,Santi SpampinatoPubMed 33584300
- Science advances 6:2020Leukemia-on-a-chip: Dissecting the chemoresistance mechanisms in B cell acute lymphoblastic leukemia bone marrow niche.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChao Ma et. al.PubMed 33127669
- Scientific reports 10:185602020HIFα independent mechanisms in renal carcinoma cells modulate divergent outcomes in fibronectin assembly mediated by hypoxia and CoCl.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCarina Magdaleno et. al.PubMed 33122751
- Journal of toxicologic pathology 33:121-1292020Hyperglycemia contributes to the development of Leydig cell hyperplasia in male Spontaneously Diabetic Torii rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYoshitomi Nakane et. al.PubMed 32425345
- Cellular physiology and biochemistry : internation 46:36-452018The Antimuscarinic Agent Tolterodine Regulates Bladder Extracellular Matrix in Partial Bladder Outlet Obstruction in Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTong-Xin Yang et. al.PubMed 29566360
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human ITGA4 aa 550-650 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P13612
Reactivity data
Select an application| Flow Cyt | IHC-P | |
|---|---|---|
| Human | Tested | Expected |
| Mouse | Expected | Tested |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20.00000 - 1/100.00000 | Notes ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100.00000 - 1/500.00000 | Notes Use at 1/50 - 1/200 with fluorescent detection methods. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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Target data
Function
Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells. ITGA4:ITGB1 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling (PubMed:23125415). ITGA4:ITGB1 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed:18635536, PubMed:25398877). Integrin ITGA4:ITGB1 represses PRKCA-mediated L-type voltage-gated channel Ca(2+) influx and ROCK-mediated calcium sensitivity in vascular smooth muscle cells via its interaction with SVEP1, thereby inhibiting vasocontraction (PubMed:35802072).
Alternative names
CD49d, CD49D, ITGA4, Integrin alpha-4, CD49 antigen-like family member D, Integrin alpha-IV, VLA-4 subunit alpha
Recommended products
Rabbit Polyclonal Integrin alpha 4/CD49D antibody. Suitable for Flow Cyt, IHC-P and reacts with Human, Mouse samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human ITGA4 aa 550-650 conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human ITGA4 aa 550-650 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P13612
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Integrin alpha 4 also known as CD49D or ITGA4 is a transmembrane receptor with a mass of approximately 150 kDa. This protein is expressed on the surface of leukocytes including T and B lymphocytes monocytes and eosinophils. Integrin alpha 4 functions by interacting mechanically with the extracellular matrix components and endothelial cell counter-receptors like VCAM-1 facilitating cell adhesion and migration. Its expression is vital for the immune cells' ability to move and locate sites of inflammation or injury.
- Biological function summary
Integrin alpha 4 forms a heterodimer complex with beta subunits like beta 1 (forming VLA-4) or beta 7 allowing it to mediate adhesion and signaling pathways. In particular the alpha 4 and beta 1 dimerization gives rise to a high-affinity receptor for fibronectin and VCAM-1. This interaction is essential for regulating immune responses and maintaining cell positioning within tissues. The presence of this complex on leukocytes drives their ability to navigate through the vasculature to inflamed areas.
- Pathways
Integrin alpha 4 participates in significant signal transduction pathways including the MAPK and PI3K-Akt pathways. These pathways modulate cell survival proliferation and migration. In the MAPK pathway integrin alpha 4 influences cell signaling cascades by interacting with other proteins like TALIN or PAXILLIN which are important for integrin-mediated signal relay. In connection with PI3K-Akt pathway alpha 4 integrins work closely with proteins in regulating cellular adhesion and motility showing a dynamic interplay in immune regulation.
- Associated diseases and disorders
Integrin alpha 4 plays an important role in various immune-related diseases such as multiple sclerosis and Crohn's disease. The protein's participation in leukocyte adhesion and trafficking makes it a target for therapeutic intervention. Natalizumab a monoclonal antibody against alpha 4 integrin showcases the critical link between the protein and disease treatment by blocking leukocyte migration to reduce inflammation. Additionally integrin alpha 4's interaction with proteins like VCAM-1 and VLA-4 highlights its relevance in these autoimmune conditions highlighting its importance in disease pathophysiology.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Flow Cytometry - Anti-Integrin alpha 4/CD49D antibody (ab202969)
Flow Cytometry analysis of Human U937 cells labeling Integrin alpha 4/CD49D with ab202969 at 0.2 μg for 30 minutes followed by incubation with a PE Conjugated secondary (green) for 30 minutes. Blue - control cells, light blue - secondary only, orange - isotype control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 4/CD49D antibody (ab202969)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Mouse lung labeling CD49d with ab202969 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.
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