Anti-Integrin alpha 4/CD49D antibody [EPR1355Y]

• WB

Lab

Western blot - Anti-Integrin alpha 4/CD49D antibody [EPR1355Y] (AB81280)

Lanes 1 - 2 : Merged signal (red and green). Green - ab81280 observed at 150 kDa. Red - loading control, ab8245, observed at 37 kDa.

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab81280 and ab8245 (loading control) overnight at 4°C. Antibody binding was detected using Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at a 1 : 10000 dilution for 1hr at room temperature and then imaged.

All lanes:

Western blot - Anti-Integrin alpha 4/CD49D antibody [EPR1355Y] (ab81280) at 1/1000 dilution

Lane 1:

Jurkat whole cell lysate at 20 µg

Lane 2:

Mouse spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 114 kDa

Observed band size: 150 kDa

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• WB

Unknown

Western blot - Anti-Integrin alpha 4/CD49D antibody [EPR1355Y] (AB81280)

All lanes:

Western blot - Anti-Integrin alpha 4/CD49D antibody [EPR1355Y] (ab81280) at 1/2000 dilution

All lanes:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti rabbit at 1/2000 dilution

Predicted band size: 114 kDa

Observed band size: 150 kDa

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• WB

Lab

Western blot - Anti-Integrin alpha 4/CD49D antibody [EPR1355Y] (AB81280)

Lane 1 : Integrin alpha 4 knockout HAP1 cell lysate (20 μg)

Lane 2 : Wild-type HAP1 cell lysate (20 μg)

Lanes 1 and 2 : Merged signal (red and green). Green - ab81280 observed at 150 kDa. Red - loading control, ab8226, observed at 42 kDa.

ab81280 was shown to recognize Integrin alpha 4 in wild-type HAP1 cells along with additional cross-reactive bands. No bands were observed when Integrin alpha 4 knockout samples were used. Wild-type and Integrin alpha 4 knockout samples were subjected to SDS-PAGE. ab81280 and ab8226 (loading control to beta actin) were diluted 1/2000 and 1/1000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1hr at room temperature before imaging.

All lanes:

Western blot - Anti-Integrin alpha 4/CD49D antibody [EPR1355Y] (ab81280)

Predicted band size: 114 kDa

false

• WB

CiteAb

Western blot - Anti-Integrin alpha 4/CD49D antibody [EPR1355Y] (AB81280)

Integrin alpha 4/CD49D western blot using anti-Integrin alpha 4/CD49D antibody [EPR1355Y] ab81280. Publication image and figure legend from Yan, J., Yang, X., et al., 2020, J Cell Mol Med, PubMed 31680442.

ab81280 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab81280 please see the product overview.

CD9/ITGA4/PI3K-Akt axis mediates trabecular meshwork cell apoptosis in human glaucoma. (A,B) Protein expression of ITGA4 in GTM3, GTM3 + CD9 and GTM3 + pcDNA3.1 groups. n = 6. (A,C) Protein expression of p-PI3K in GTM3, GTM3 + CD9 and GTM3 + pcDNA3.1 groups. n = 6. (A,D) Protein expression of p-Akt in GTM3, GTM3 + CD9 and GTM3 + pcDNA3.1 groups. n = 6. (A,E) Protein expression of clevage caspase3 in GTM3, GTM3 + CD9 and GTM3 + pcDNA3.1 groups. n = 6. *P < .05 vs iHTM group, #P < .05 vs GTM3 group. (F-G) FCAS results of cells from iHTM, GTM3, GTM3 + CD9 and GTM3 + pcDNA3.1 groups. n = 3. *P < .05 vs iHTM group, #P < .05 vs GTM3 group

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