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AB22858

Anti-Integrin alpha 4/CD49D antibody [HP2/1]

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(6 Publications)

Mouse Monoclonal Integrin alpha 4/CD49D antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Cell preparation containing ITGA4 protein.

View Alternative Names

CD49d, CD49D, ITGA4, Integrin alpha-4, CD49 antigen-like family member D, Integrin alpha-IV, VLA-4 subunit alpha

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha 4/CD49D antibody [HP2/1] (AB22858)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha 4/CD49D antibody [HP2/1] (AB22858)

ab22858 staining Integrin alpha 4/CD49D in wild-type HAP1 cells (top panel) and Integrin alpha 4/CD49D knockout HAP1 cells (bottom panel). The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab22858 at 5μg/ml concentration and ab202272 at 1/250 dilution (shown in pseudo colour red) overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to Mouse IgG (Alexa Fluor® 488) (ab150117) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

HP2/1

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Cell preparation containing ITGA4 protein. The exact immunogen used to generate this antibody is proprietary information.

P13612

Specificity

This antibody recognises the 80kD alpha chain of VLA-4. Reacts with monocytes, T cells, B cells, thymocytes and Langerhans cells.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Can be used in basic studies of VLA-4 mediated adhesion and its interaction with the VCAM-1 structure. HP2/1 inhibits cell binding to soluble VCAM-1.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Integrin alpha 4 also known as CD49D or ITGA4 is a transmembrane receptor with a mass of approximately 150 kDa. This protein is expressed on the surface of leukocytes including T and B lymphocytes monocytes and eosinophils. Integrin alpha 4 functions by interacting mechanically with the extracellular matrix components and endothelial cell counter-receptors like VCAM-1 facilitating cell adhesion and migration. Its expression is vital for the immune cells' ability to move and locate sites of inflammation or injury.
Biological function summary

Integrin alpha 4 forms a heterodimer complex with beta subunits like beta 1 (forming VLA-4) or beta 7 allowing it to mediate adhesion and signaling pathways. In particular the alpha 4 and beta 1 dimerization gives rise to a high-affinity receptor for fibronectin and VCAM-1. This interaction is essential for regulating immune responses and maintaining cell positioning within tissues. The presence of this complex on leukocytes drives their ability to navigate through the vasculature to inflamed areas.

Pathways

Integrin alpha 4 participates in significant signal transduction pathways including the MAPK and PI3K-Akt pathways. These pathways modulate cell survival proliferation and migration. In the MAPK pathway integrin alpha 4 influences cell signaling cascades by interacting with other proteins like TALIN or PAXILLIN which are important for integrin-mediated signal relay. In connection with PI3K-Akt pathway alpha 4 integrins work closely with proteins in regulating cellular adhesion and motility showing a dynamic interplay in immune regulation.

Integrin alpha 4 plays an important role in various immune-related diseases such as multiple sclerosis and Crohn's disease. The protein's participation in leukocyte adhesion and trafficking makes it a target for therapeutic intervention. Natalizumab a monoclonal antibody against alpha 4 integrin showcases the critical link between the protein and disease treatment by blocking leukocyte migration to reduce inflammation. Additionally integrin alpha 4's interaction with proteins like VCAM-1 and VLA-4 highlights its relevance in these autoimmune conditions highlighting its importance in disease pathophysiology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells. ITGA4 : ITGB1 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling (PubMed : 23125415). ITGA4 : ITGB1 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed : 18635536, PubMed : 25398877). Integrin ITGA4 : ITGB1 represses PRKCA-mediated L-type voltage-gated channel Ca(2+) influx and ROCK-mediated calcium sensitivity in vascular smooth muscle cells via its interaction with SVEP1, thereby inhibiting vasocontraction (PubMed : 35802072).
See full target information ITGA4
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Glia 69:2199-2214 PubMed33991013

2021

Elevated fibroblast growth factor-inducible 14 expression transforms proneural-like gliomas into more aggressive and lethal brain cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Nina P Connolly,Rebeca Galisteo,Su Xu,Eli E Bar,Sen Peng,Nhan L Tran,Heather M Ames,Anthony J Kim,Graeme F Woodworth,Jeffrey A Winkles

Veterinary research 50:101 PubMed31783923

2019

Oral vitamin A supplementation of porcine epidemic diarrhea virus infected gilts enhances IgA and lactogenic immune protection of nursing piglets.

Applications

Unspecified application

Species

Unspecified reactive species

Stephanie N Langel,Francine Chimelo Paim,Moyasar A Alhamo,Kelly M Lager,Anastasia N Vlasova,Linda J Saif

Frontiers in immunology 10:727 PubMed31068924

2019

Stage of Gestation at Porcine Epidemic Diarrhea Virus Infection of Pregnant Swine Impacts Maternal Immunity and Lactogenic Immune Protection of Neonatal Suckling Piglets.

Applications

Unspecified application

Species

Unspecified reactive species

Stephanie N Langel,Francine C Paim,Moyasar A Alhamo,Alexandra Buckley,Albert Van Geelen,Kelly M Lager,Anastasia N Vlasova,Linda J Saif

Cancer research 78:2305-2317 PubMed29440144

2018

SPON2 Promotes M1-like Macrophage Recruitment and Inhibits Hepatocellular Carcinoma Metastasis by Distinct Integrin-Rho GTPase-Hippo Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Yan-Li Zhang,Qing Li,Xiao-Mei Yang,Fang Fang,Jun Li,Ya-Hui Wang,Qin Yang,Lei Zhu,Hui-Zhen Nie,Xue-Li Zhang,Ming-Xuan Feng,Shu-Heng Jiang,Guang-Ang Tian,Li-Peng Hu,Ho-Young Lee,Su-Jae Lee,Qiang Xia,Zhi-Gang Zhang

PloS one 12:e0174557 PubMed28358926

2017

Genetically engineered rat gliomas: PDGF-driven tumor initiation and progression in tv-a transgenic rats recreate key features of human brain cancer.

Applications

IHC-Fr

Species

Rat

Nina P Connolly,Jesse A Stokum,Craig S Schneider,Tatsuya Ozawa,Su Xu,Rebeca Galisteo,Rudolph J Castellani,Anthony J Kim,J Marc Simard,Jeffrey A Winkles,Eric C Holland,Graeme F Woodworth

The Journal of biological chemistry 287:15749-59 PubMed22418441

2012

Identification, characterization, and epitope mapping of human monoclonal antibody J19 that specifically recognizes activated integrin α4β7.

Applications

Flow Cyt

Species

Human

JunPeng Qi,Kun Zhang,Qiao Zhang,Yi Sun,Ting Fu,GuoHui Li,JianFeng Chen
View all publications
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