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AB73261

Anti-Integrin alpha 4+beta 7 antibody [RM0059-2G18]

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(2 Publications)

Rat Monoclonal Integrin alpha 4/CD49D antibody. Suitable for IP, Flow Cyt, IHC-P and reacts with Mouse samples. Cited in 2 publications. Immunogen corresponding to Cell preparation containing Itga4 protein.

View Alternative Names

CD49d, Integrin alpha-4, CD49 antigen-like family member D, Integrin alpha-IV, Lymphocyte Peyer patch adhesion molecules subunit alpha, VLA-4 subunit alpha, LPAM subunit alpha, Itga4, Integrin beta-7, Integrin beta-P, M290 IEL antigen, Itgb7

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 4+beta 7 antibody [RM0059-2G18] (AB73261)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 4+beta 7 antibody [RM0059-2G18] (AB73261)

ab73261 at 1/200 dilution, staining Integrin alpha 4+beta 7 in LPS exposed mouse spleen tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections).

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

RM0059-2G18

Isotype

IgG2a

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt, IHC-P, IP

applications

Immunogen

Cell preparation containing Itga4 protein. The exact immunogen used to generate this antibody is proprietary information.

Q00651

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Secondary Antibodies

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Primary Antibodies

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Primary Antibodies

AB179471

Anti-Integrin beta 1 antibody [EPR16895]

RabMAb|Recombinant|KO Validated

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  • 5
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/200", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Integrin alpha 4 beta 7 also known as alpha 4 beta 7 integrin A4B7 integrin and beta-7 is a cell surface integrin receptor involved in mediating cell adhesion. This target plays a mechanical role in binding to the mucosal addressin cell adhesion molecule-1 (MAdCAM-1) primarily on high endothelial venules in the gut-associated lymphoid tissue. It forms a heterodimer composed of the alpha 4 and beta 7 subunits with an estimated mass of about 150–180 kDa. Integrin alpha 4 beta 7 is expressed on the surface of leukocytes including T cells B cells and other immune cells facilitating their tissue-specific homing.
Biological function summary

Integrin alpha 4 beta 7 serves an important role in the migration and homing of lymphocytes to gut-associated lymphoid tissues ensuring immune surveillance. It is a part of the integrin family complex interacting with MAdCAM-1 to guide lymphocytes across the endothelium into the intestinal lamina propria. This process is essential for maintaining the immune cell balance within the gastrointestinal tract where the immune system encounters numerous antigens.

Pathways

Integrin alpha 4 beta 7 fits into immune cell trafficking and adhesion pathways. It works closely with proteins like MAdCAM-1 and interacts with the Janus kinase-signal transducer and activator of transcription (JAK-STAT) signaling pathway. These interactions reinforce the pivotal role of integrin in the precise targeting and localization of immune cells which is necessary for effective immune responses and homeostasis maintenance within specific tissue environments.

Integrin alpha 4 beta 7 connects with conditions such as inflammatory bowel diseases including Crohn's disease and ulcerative colitis. Its interaction with MAdCAM-1 contributes to the recruitment of inflammatory cells to the gut exacerbating inflammation in these disorders. Moreover integrin alpha 4 beta 7 has been implicated in HIV infection where it serves as a marker for lymphocytes targeted by the virus highlighting its potential as a therapeutic target for modulating immune system activity in disease contexts.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Integrins alpha-4/beta-1 (VLA-4 or LPAM-2) and alpha-4/beta-7 (LPAM-1) are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells. ITGA4 : ITGB1 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling. ITGA4 : ITGB1 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1. Integrin ITGA4 : ITGB1 represses PRKCA-mediated L-type voltage-gated channel Ca(2+) influx and ROCK-mediated calcium sensitivity in vascular smooth muscle cells via its interaction with SVEP1, thereby inhibiting vasocontraction (PubMed : 35802072).
See full target information Itga4

Additional targets

Itgb7
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Medicina (Kaunas, Lithuania) 59: PubMed36676712

2022

Effects of the Antioxidant Quercetin in an Experimental Model of Ulcerative Colitis in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

George Kottakis,Katerina Kambouri,Alexandra Giatromanolaki,Georgia Valsami,Nikolaos Kostomitsopoulos,Alexandra Tsaroucha,Michael Pitiakoudis

Journal of materials science. Materials in medicine 33:19 PubMed35072831

2022

Loading of erythropoietin on biphasic calcium phosphate bioceramics promotes osteogenesis and angiogenesis by regulating EphB4/EphrinB2 molecules.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Wang,Peng Wang,Qionghui Wu,Zhifan Qin,Zichao Xiang,Yuxian Chu,Jihua Li
View all publications
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Product promise

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