AB25251

Anti-Integrin alpha 5 antibody [5H10-27 (MFR5)]

Name: Anti-Integrin alpha 5 antibody [5H10-27 (MFR5)] (ab25251) | Abcam
Brand: Abcam Limited
SKU: AB25251
Price: 565 USD
Availability: InStock
Rating: 4 (2 reviews)

(2 Reviews)

(10 Publications)

Rat Monoclonal Integrin alpha 5 antibody. Suitable for Flow Cyt, FuncS, ICC/IF, IHC-Fr and reacts with Mouse samples. Cited in 10 publications. Immunogen corresponding to Cell preparation containing Integrin alpha-5 protein.

View Alternative Names

CD49e, Integrin alpha-5, CD49 antigen-like family member E, Fibronectin receptor subunit alpha, Integrin alpha-F, VLA-5, Itga5

1 Images

Flow Cytometry - Anti-Integrin alpha 5 antibody [5H10-27 (MFR5)] (AB25251)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-Integrin alpha 5 antibody [5H10-27 (MFR5)] (AB25251)

C57BL/6 mouse bone marrow cells were stained with Rat Anti-Mouse CD49e-UNLB followed by Goat Anti-Rat IgG(H+L), Mouse ads-PE. X‑axis (horizontal) : Fluorescence Intensity (PE). Y‑axis (vertical) : Cell Count. The grey peak is the isotype control (Rat IgG2a-UNLB) while the purple peak is the positive dataset.

519 FITC

FITC Anti-Integrin alpha-5 antibody [5H10-27 (MFR5)]
578 PE

PE Anti-Integrin alpha-5 antibody [5H10-27 (MFR5)]

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

5H10-27 (MFR5)

Isotype

IgG2a

Light chain type

kappa

Carrier free

Reacts with

Mouse

Applications

FuncS, ICC/IF, Flow Cyt, IHC-Fr

applications

Immunogen

Cell preparation containing Integrin alpha-5 protein. The exact immunogen used to generate this antibody is proprietary information.

P11688

Specificity

ab25251 is specific for the alpha 5 subunit of the integrin alpha 5 beta 1 fibronectin receptor.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "FuncS" : {"fullname" : "Functional Studies", "shortname":"FuncS"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "Use up to 1ug/10^6 cells. <a href='/en-us/products/primary-antibodies/rat-igg2a-kappa-monoclonal-rtk2758-isotype-control-ab18450'>ab18450</a> - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.", "FuncS-species-checked": "guaranteed", "FuncS-species-dilution-info": "", "FuncS-species-notes": "T-cell co-stimulation, In vitro blocking of adhesion.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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Product details

Soluble ab25251 inhibits VLA5-mediated functions in vitro. Immobilized ab25251 has also been demonstrated to co-stimulate the proliferative response of CD8+ T cells to plate-bound antiCD3 monoclonal antibody.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 8.2 Constituents: 100% Borate buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Integrin alpha 5 also known as CD49e or ITGA5 is a subunit of the integrin family that forms heterodimers with beta 1 integrin. It has an approximate mass of 130 kDa. This protein mainly expresses in various tissues including fibroblasts endothelial cells and certain epithelial cells. Integrin alpha 5 mediates cell adhesion by binding to fibronectin an important extracellular matrix protein helping cells stay attached to their surrounding environment.

Biological function summary

Integrin alpha 5 plays a significant role in cellular processes such as migration proliferation and differentiation. It pairs with beta 1 integrin to form the alpha 5 beta 1 integrin complex. This complex facilitates the transmission of mechanical and chemical signals between the extracellular matrix and the cell interior. Integrin alpha 5 also interacts with intracellular proteins like talin and kindlin which are essential for initiating and strengthening the linkage to the actin cytoskeleton.

Pathways

Integrin alpha 5 is involved in the regulation of several critical signaling pathways. It prominently features within the MAPK/ERK pathway and the focal adhesion signaling pathway. Through these pathways integrin alpha 5 influences cell survival proliferation and migration. It interacts with proteins such as paxillin and focal adhesion kinase (FAK) within these pathways contributing to cellular responses to external stimuli.

Integrin alpha 5 is linked to conditions such as cancer and fibrosis. Overexpression or aberrant regulation of this integrin often associates with tumorigenesis particularly in breast and colorectal cancers enhancing cancer cell migration and invasion. Integrin alpha 5 is also connected to proteins like matrix metalloproteinases (MMPs) which play roles in extracellular matrix remodeling and are involved in fibrotic diseases. These associations highlight the importance of integrin alpha 5 in pathological processes and its potential as a target for therapeutic intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Integrin alpha-5/beta-1 (ITGA5 : ITGB1) is a receptor for fibronectin and fibrinogen (PubMed : 36812915). It recognizes the sequence R-G-D in its ligands. ITGA5 : ITGB1 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1. ITGA5 : ITGB1 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1. ITGA5 : ITGB1 acts as a receptor for fibronectin (FN1) and mediates R-G-D-dependent cell adhesion to FN1 (By similarity). ITGA5 : ITGB1 is a receptor for IL1B and binding is essential for IL1B signaling. ITGA5 : ITGB3 is a receptor for soluble CD40LG and is required for CD40/CD40LG signaling (By similarity).

See full target information Integrin alpha-5

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Journal of biomedical materials research. Part A 108:923-937 PubMed31895490

2020

RGD-functionalized supported lipid bilayers modulate pre-osteoblast adherence and promote osteogenic differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Johanna F M Verstappen,Jianfeng Jin,Gülistan Koçer,Mohammad Haroon,Pascal Jonkheijm,Astrid D Bakker,Jenneke Klein-Nulend,Richard T Jaspers

International journal of molecular sciences 20: PubMed31252620

2019

The Psoriasis Therapeutic Potential of a Novel Short Laminin Peptide C16.

Applications

Unspecified application

Species

Unspecified reactive species

Tsung-Chuan Ho,Shu-I Yeh,Show-Li Chen,Yeou-Ping Tsao

International journal of molecular medicine 38:737-46 PubMed27460641

2016

Knockdown of HMGN2 increases the internalization of Klebsiella pneumoniae by respiratory epithelial cells through the regulation of α5β1 integrin expression.

Applications

Unspecified application

Species

Human

Xinyuan Wang,Jingyu Li,Shanze Chen,Xiaofei Shen,Xiaolong Yang,Yan Teng,Luxia Deng,Yi Wang,Junli Chen,Xiaoying Wang,Ning Huang

Osteoarthritis and cartilage 24:534-43 PubMed26497608

2015

Detecting new microRNAs in human osteoarthritic chondrocytes identifies miR-3085 as a human, chondrocyte-selective, microRNA.

Applications

Unspecified application

Species

Unspecified reactive species

N Crowe,T E Swingler,L T T Le,M J Barter,G Wheeler,H Pais,S T Donell,D A Young,T Dalmay,I M Clark

Journal of cell science 125:1666-72 PubMed22328507

2012

Involvement of actin polymerization in podosome dynamics.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Luxenburg,Sabina Winograd-Katz,Lia Addadi,Benjamin Geiger

Journal of immunology (Baltimore, Md. : 1950) 157:2916-23 PubMed8816397

1996

Role of alpha 5 beta 1 integrin in TGF-beta 1-costimulated CD8+ T cell growth and apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

S Rich,N Van Nood,H M Lee

Clinical & experimental metastasis 14:125-37 PubMed8605726

1996

Attachment, invasion, chemotaxis, and proteinase expression of B16-BL6 melanoma cells exhibiting a low metastatic phenotype after exposure to dietary restriction of tyrosine and phenylalanine.

Applications

Unspecified application

Species

Unspecified reactive species

C E Uhlenkott,J C Huijzer,D J Cardeiro,C A Elstad,G G Meadows

Journal of immunology (Baltimore, Md. : 1950) 155:4567-74 PubMed7594454

1995

Enhancement of VLA integrin receptor function on thymocytes by cAMP is dependent on the maturation stage of the thymocytes.

Applications

Unspecified application

Species

Unspecified reactive species

M J Halvorson,J E Coligan

The Journal of biological chemistry 270:11522-31 PubMed7538116

1995

Beta 1- and beta 3-class integrins mediate fibronectin binding activity at the surface of developing mouse peri-implantation blastocysts. Regulation by ligand-induced mobilization of stored receptor.

Applications

Unspecified application

Species

Unspecified reactive species

J F Schultz,D R Armant

Blood cells 20:25-44 PubMed7527678

1994

Adhesion molecules in hematopoietic cells.

Applications

Unspecified application

Species

Unspecified reactive species

T Kinashi,T A Springer

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Integrin alpha 5 antibody [5H10-27 (MFR5)]

Flow Cytometry - Anti-Integrin alpha 5 antibody [5H10-27 (MFR5)] (AB25251)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (10)

Product promise