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AB203029

Anti-Integrin alpha 5 antibody

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Rabbit Polyclonal Integrin alpha 5 antibody. Suitable for IHC-P and reacts with Mouse samples. Immunogen corresponding to Synthetic Peptide within Human ITGA5 aa 1000 to C-terminus conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

CD49e, FNRA, ITGA5, Integrin alpha-5, CD49 antigen-like family member E, Fibronectin receptor subunit alpha, Integrin alpha-F, VLA-5

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 5 antibody (AB203029)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 5 antibody (AB203029)

Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded mouse placenta tissue labeling Integrin alpha 5 using ab203029 at a 1/400 dilution, followed by a conjugated secondary and DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 5 antibody (AB203029)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 5 antibody (AB203029)

Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded mouse placenta tissue labeling Integrin alpha 5 using ab203029 at a 1/400 dilution, followed by a conjugated secondary and DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 5 antibody (AB203029)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 5 antibody (AB203029)

Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded mouse placenta tissue labeling Integrin alpha 5 using ab203029 at a 1/200 dilution, followed by a conjugated secondary and DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 5 antibody (AB203029)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 5 antibody (AB203029)

Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded mouse placenta tissue labeling Integrin alpha 5 using ab203029 at a 1/400 dilution, followed by a conjugated secondary and DAB staining.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human ITGA5 aa 1000 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P08648

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Integrin alpha 5 also known as CD49e or ITGA5 is a subunit of the integrin family that forms heterodimers with beta 1 integrin. It has an approximate mass of 130 kDa. This protein mainly expresses in various tissues including fibroblasts endothelial cells and certain epithelial cells. Integrin alpha 5 mediates cell adhesion by binding to fibronectin an important extracellular matrix protein helping cells stay attached to their surrounding environment.
Biological function summary

Integrin alpha 5 plays a significant role in cellular processes such as migration proliferation and differentiation. It pairs with beta 1 integrin to form the alpha 5 beta 1 integrin complex. This complex facilitates the transmission of mechanical and chemical signals between the extracellular matrix and the cell interior. Integrin alpha 5 also interacts with intracellular proteins like talin and kindlin which are essential for initiating and strengthening the linkage to the actin cytoskeleton.

Pathways

Integrin alpha 5 is involved in the regulation of several critical signaling pathways. It prominently features within the MAPK/ERK pathway and the focal adhesion signaling pathway. Through these pathways integrin alpha 5 influences cell survival proliferation and migration. It interacts with proteins such as paxillin and focal adhesion kinase (FAK) within these pathways contributing to cellular responses to external stimuli.

Integrin alpha 5 is linked to conditions such as cancer and fibrosis. Overexpression or aberrant regulation of this integrin often associates with tumorigenesis particularly in breast and colorectal cancers enhancing cancer cell migration and invasion. Integrin alpha 5 is also connected to proteins like matrix metalloproteinases (MMPs) which play roles in extracellular matrix remodeling and are involved in fibrotic diseases. These associations highlight the importance of integrin alpha 5 in pathological processes and its potential as a target for therapeutic intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Integrin alpha-5/beta-1 (ITGA5 : ITGB1) is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. ITGA5 : ITGB1 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed : 18635536, PubMed : 25398877). ITGA5 : ITGB1 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1 (PubMed : 12807887, PubMed : 17158881). ITGA5 : ITGB1 acts as a receptor for fibronectin (FN1) and mediates R-G-D-dependent cell adhesion to FN1 (PubMed : 33962943). ITGA5 : ITGB1 is a receptor for IL1B and binding is essential for IL1B signaling (PubMed : 29030430). ITGA5 : ITGB3 is a receptor for soluble CD40LG and is required for CD40/CD40LG signaling (PubMed : 31331973).. (Microbial infection) Integrin ITGA5 : ITGB1 acts as a receptor for Human metapneumovirus.. (Microbial infection) Integrin ITGA2 : ITGB1 acts as a receptor for Human parvovirus B19.. (Microbial infection) In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
See full target information ITGA5
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