Rabbit Polyclonal Integrin alpha 5 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ITGA5.
Images
Publications
Filter by
- OncoTargets and therapy 13:5323-53352020Connexin 43 Modulates the Cellular Resistance to Paclitaxel via Targeting β-Tubulin in Triple-Negative Breast Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYun Fu et. al.PubMed 32606750
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ITGA5. The exact immunogen used to generate this antibody is proprietary information. Database link P08648
Consider this alternative
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Tested |
| Rat | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 - 1/10000 | Notes - |
Species Rat | Dilution info 1/1000 - 1/10000 | Notes - |
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Integrin alpha-5/beta-1 (ITGA5:ITGB1) is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. ITGA5:ITGB1 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed:18635536, PubMed:25398877). ITGA5:ITGB1 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1 (PubMed:12807887, PubMed:17158881). ITGA5:ITGB1 is a receptor for IL1B and binding is essential for IL1B signaling (PubMed:29030430). ITGA5:ITGB3 is a receptor for soluble CD40LG and is required for CD40/CD40LG signaling (PubMed:31331973).(Microbial infection) Integrin ITGA5:ITGB1 acts as a receptor for Human metapneumovirus.(Microbial infection) Integrin ITGA2:ITGB1 acts as a receptor for Human parvovirus B19.(Microbial infection) In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
Alternative names
Integrin alpha-5, CD49 antigen-like family member E, Fibronectin receptor subunit alpha, Integrin alpha-F, VLA-5, FNRA, ITGA5
Recommended products
Rabbit Polyclonal Integrin alpha 5 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ITGA5.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ITGA5. The exact immunogen used to generate this antibody is proprietary information. Database link P08648
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Integrin alpha 5 also known as CD49e or ITGA5 is a subunit of the integrin family that forms heterodimers with beta 1 integrin. It has an approximate mass of 130 kDa. This protein mainly expresses in various tissues including fibroblasts endothelial cells and certain epithelial cells. Integrin alpha 5 mediates cell adhesion by binding to fibronectin an important extracellular matrix protein helping cells stay attached to their surrounding environment.
- Biological function summary
Integrin alpha 5 plays a significant role in cellular processes such as migration proliferation and differentiation. It pairs with beta 1 integrin to form the alpha 5 beta 1 integrin complex. This complex facilitates the transmission of mechanical and chemical signals between the extracellular matrix and the cell interior. Integrin alpha 5 also interacts with intracellular proteins like talin and kindlin which are essential for initiating and strengthening the linkage to the actin cytoskeleton.
- Pathways
Integrin alpha 5 is involved in the regulation of several critical signaling pathways. It prominently features within the MAPK/ERK pathway and the focal adhesion signaling pathway. Through these pathways integrin alpha 5 influences cell survival proliferation and migration. It interacts with proteins such as paxillin and focal adhesion kinase (FAK) within these pathways contributing to cellular responses to external stimuli.
- Associated diseases and disorders
Integrin alpha 5 is linked to conditions such as cancer and fibrosis. Overexpression or aberrant regulation of this integrin often associates with tumorigenesis particularly in breast and colorectal cancers enhancing cancer cell migration and invasion. Integrin alpha 5 is also connected to proteins like matrix metalloproteinases (MMPs) which play roles in extracellular matrix remodeling and are involved in fibrotic diseases. These associations highlight the importance of integrin alpha 5 in pathological processes and its potential as a target for therapeutic intervention.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Western blot - Anti-Integrin alpha 5 antibody (ab226816)
All lanes: Western blot - Anti-Integrin alpha 5 antibody (ab226816) at 1/5000 dilution
Lane 1: MDA-MB-231 (human breast adenocarcinoma cell line) cell lysate at 30 µg
Lane 2: MDA-MB-231 membrane extract lysate at 30 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 115 kDa, 47 kDa
Western blot - Anti-Integrin alpha 5 antibody (ab226816)
All lanes: Western blot - Anti-Integrin alpha 5 antibody (ab226816) at 1/5000 dilution
All lanes: Mouse liver tissue lysate at 50 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 115 kDa
Western blot - Anti-Integrin alpha 5 antibody (ab226816)
All lanes: Western blot - Anti-Integrin alpha 5 antibody (ab226816) at 1/5000 dilution
All lanes: Rat liver tissue lysate at 50 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 115 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com