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AB221606

Anti-Integrin alpha 5 antibody [EPR7854] - Low endotoxin, Azide free

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(3 Publications)

Rabbit Recombinant Monoclonal Integrin alpha 5 antibody. Carrier free. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 3 publications.

View Alternative Names

CD49e, FNRA, ITGA5, Integrin alpha-5, CD49 antigen-like family member E, Fibronectin receptor subunit alpha, Integrin alpha-F, VLA-5

17 Images
Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha 5 antibody [EPR7854] - Low endotoxin, Azide free (AB221606)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha 5 antibody [EPR7854] - Low endotoxin, Azide free (AB221606)

Immunocytochemistry/ Immunofluorescence analysis of U937 (Human histiocytic lymphoma monocyte) cells labeling Integrin alpha 5 with purified ab150361 at 1 : 50 dilution (4.3 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1 : 200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab150361)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 5 antibody [EPR7854] - Low endotoxin, Azide free (AB221606)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 5 antibody [EPR7854] - Low endotoxin, Azide free (AB221606)

Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling Integrin alpha 5 with unpurified ab150361 antibody at a dilution of 1/100.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab150361).

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-Integrin alpha 5 antibody [EPR7854] - Low endotoxin, Azide free (AB221606)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Integrin alpha 5 antibody [EPR7854] - Low endotoxin, Azide free (AB221606)

Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Integrin alpha 5 with purified ab150361 at 1/20 dilution (10 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab150361)

Flow Cytometry (Intracellular) - Anti-Integrin alpha 5 antibody [EPR7854] - Low endotoxin, Azide free (AB221606)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Integrin alpha 5 antibody [EPR7854] - Low endotoxin, Azide free (AB221606)

Overlay histogram showing HeLa cells stained with unpurified ab150361 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab150361, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was goat anti-rabbit Alexa Fluor 488 IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab150361).

Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha 5 antibody [EPR7854] - Low endotoxin, Azide free (AB221606)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha 5 antibody [EPR7854] - Low endotoxin, Azide free (AB221606)

ab150361 staining Integrin alpha 5 in MCF7 cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab150361 at 1μg/ml concentration and ab7291 at 1μg/ml concentration overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to Rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green) and a goat secondary antibody to Mouse IgG (Alexa Fluor® 594) (ab150120) at 2 μg/ml (showed in pseudocolour red) . Nuclear DNA was labelled in blue with DAPI.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab150361).

Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha 5 antibody [EPR7854] - Low endotoxin, Azide free (AB221606)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha 5 antibody [EPR7854] - Low endotoxin, Azide free (AB221606)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U937 (Human histiocytic lymphoma) cells labeling Integrin alpha 5 with unpurified ab150361 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor 488) (ab150077) secondary antibody at 1/400 dilution (green). Confocal image showing mainly membrane staining on U937 cell line. The nuclear counterstain is DAPI (blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab150361).

Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha 5 antibody [EPR7854] - Low endotoxin, Azide free (AB221606)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha 5 antibody [EPR7854] - Low endotoxin, Azide free (AB221606)

ab150361 staining Integrin alpha 5 in wild-type HAP1 cells (top panel) and ITGA5 knockout HAP1 cells (bottom panel). The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab150361 at 1/250 dilution and ab7291 at 1μg/ml concentration overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to Rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green) and a goat secondary antibody to Mouse IgG (Alexa Fluor® 594) (ab150120) at 2 μg/ml (showed in pseudocolour red) . Nuclear DNA was labelled in blue with DAPI.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab150361).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 5 antibody [EPR7854] - Low endotoxin, Azide free (AB221606)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 5 antibody [EPR7854] - Low endotoxin, Azide free (AB221606)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebral cortex tissue sections labeling Integrin alpha 5 with purified ab150361 at 1 : 400 dilution (0.54 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab150361)

Immunoprecipitation - Anti-Integrin alpha 5 antibody [EPR7854] - Low endotoxin, Azide free (AB221606)
  • IP

Unknown

Immunoprecipitation - Anti-Integrin alpha 5 antibody [EPR7854] - Low endotoxin, Azide free (AB221606)

ab150361 (Purified) at 1 : 20 dilution (1 µg) immunoprecipitating Integrin alpha 5 in HeLa whole cell lysate.
Lane 1 (input) : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10 µg
Lane 2 (+) : ab150361 & HeLa whole cell lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab150361 in HeLa whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1 : 5000 dilution.
Blocking and diluting buffer : 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab150361)

All lanes:

Immunoprecipitation - Anti-Integrin alpha 5 antibody [EPR7854] (<a href='/en-us/products/primary-antibodies/integrin-alpha-5-antibody-epr7854-ab150361'>ab150361</a>)

Predicted band size: 115 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 5 antibody [EPR7854] - Low endotoxin, Azide free (AB221606)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 5 antibody [EPR7854] - Low endotoxin, Azide free (AB221606)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebral cortex tissue sections labeling Integrin alpha 5 with purified ab150361 at 1 : 400 dilution (0.54 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab150361)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 5 antibody [EPR7854] - Low endotoxin, Azide free (AB221606)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 5 antibody [EPR7854] - Low endotoxin, Azide free (AB221606)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebral cortex tissue sections labeling Integrin alpha 5 with purified ab150361 at 1 : 400 dilution (0.54 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab150361)

Western blot - Anti-Integrin alpha 5 antibody [EPR7854] - Low endotoxin, Azide free (AB221606)
  • WB

Lab

Western blot - Anti-Integrin alpha 5 antibody [EPR7854] - Low endotoxin, Azide free (AB221606)

The bands detected by ab150361 are consistent with what have been described in PMID : 22740495. Full length : 150kDa; Light chain : 19kDa.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab150361).

All lanes:

Western blot - Anti-Integrin alpha 5 antibody [EPR7854] (<a href='/en-us/products/primary-antibodies/integrin-alpha-5-antibody-epr7854-ab150361'>ab150361</a>) at 1/10000 dilution

All lanes:

HT-1080 (Human fibrosarcoma cell line) whole cell lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 115 kDa

Observed band size: 150 kDa,19 kDa

false

Western blot - Anti-Integrin alpha 5 antibody [EPR7854] - Low endotoxin, Azide free (AB221606)
  • WB

Lab

Western blot - Anti-Integrin alpha 5 antibody [EPR7854] - Low endotoxin, Azide free (AB221606)

Observed band size : 19, 150 kDa

Exposure time : Lane 1-2 : 1 minutes, Lane 3-4 : 3 minutes

Blocking/Diluting buffer 5% NFDM/TBST

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab150361).

All lanes:

Western blot - Anti-Integrin alpha 5 antibody [EPR7854] (<a href='/en-us/products/primary-antibodies/integrin-alpha-5-antibody-epr7854-ab150361'>ab150361</a>) at 1/2000 dilution

Lane 1:

Human bladder tissue lysate at 10 µg

Lane 2:

HT-1080 (Human urinary bladder carcinoma cell line) whole cell lysates at 10 µg

Lane 3:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysates at 10 µg

Lane 4:

U937 (Human histiocytic lymphoma cell line) whole cell lysates at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 115 kDa

false

Western blot - Anti-Integrin alpha 5 antibody [EPR7854] - Low endotoxin, Azide free (AB221606)
  • WB

Lab

Western blot - Anti-Integrin alpha 5 antibody [EPR7854] - Low endotoxin, Azide free (AB221606)

The bands detected by ab150361 are consistent with what have been described in PMID : 22740495. Full length : 150kDa; Light chain : 19kDa.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab150361).

Lane 1:

Western blot - Anti-Integrin alpha 5 antibody [EPR7854] (<a href='/en-us/products/primary-antibodies/integrin-alpha-5-antibody-epr7854-ab150361'>ab150361</a>) at 1/1000 dilution

Lane 1:

Western blot - Anti-Integrin alpha 5 antibody [EPR7854] - Low endotoxin, Azide free (ab221606)

All lanes:

U937 (Human histiocytic lymphoma cell line) whole cell lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 115 kDa

Observed band size: 150 kDa,19 kDa

false

Western blot - Anti-Integrin alpha 5 antibody [EPR7854] - Low endotoxin, Azide free (AB221606)
  • WB

Lab

Western blot - Anti-Integrin alpha 5 antibody [EPR7854] - Low endotoxin, Azide free (AB221606)

Observed band size : 19, 150 kDa

Exposure time : 3 minutes

Blocking/Diluting buffer 5% NFDM/TBST

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab150361).

All lanes:

Western blot - Anti-Integrin alpha 5 antibody [EPR7854] (<a href='/en-us/products/primary-antibodies/integrin-alpha-5-antibody-epr7854-ab150361'>ab150361</a>) at 1/2000 dilution

Lane 1:

Mouse heart tissue lysate at 10 µg

Lane 2:

Rat heart tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 115 kDa

false

Western blot - Anti-Integrin alpha 5 antibody [EPR7854] - Low endotoxin, Azide free (AB221606)
  • WB

Lab

Western blot - Anti-Integrin alpha 5 antibody [EPR7854] - Low endotoxin, Azide free (AB221606)

The bands detected by ab150361 are consistent with what have been described in PMID : 22740495. Full length : 150kDa; Light chain : 19kDa.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab150361).

All lanes:

Western blot - Anti-Integrin alpha 5 antibody [EPR7854] (<a href='/en-us/products/primary-antibodies/integrin-alpha-5-antibody-epr7854-ab150361'>ab150361</a>) at 1/1000 dilution

Lane 1:

Rat1 (Rat fibroblast cell line) whole cell lysates at 20 µg

Lane 2:

Rat liver lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 115 kDa

Observed band size: 150 kDa,19 kDa

false

Western blot - Anti-Integrin alpha 5 antibody [EPR7854] - Low endotoxin, Azide free (AB221606)
  • WB

Lab

Western blot - Anti-Integrin alpha 5 antibody [EPR7854] - Low endotoxin, Azide free (AB221606)

The bands detected by ab150361 are consistent with what have been described in PMID : 22740495. Full length : 150kDa; Light chain : 19kDa.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab150361).

All lanes:

Western blot - Anti-Integrin alpha 5 antibody [EPR7854] (<a href='/en-us/products/primary-antibodies/integrin-alpha-5-antibody-epr7854-ab150361'>ab150361</a>) at 1/1000 dilution

Lane 1:

NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysates at 20 µg

Lane 2:

MEF (Mouse embryonic fibroblast cell line) whole cell lysates at 20 µg

Lane 3:

C2C12 (Mouse myoblast cell line) whole cell lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 115 kDa

Observed band size: 150 kDa,19 kDa

false

  • Unconjugated

    Anti-Integrin alpha 5 antibody [EPR7854]

  • Carrier free

    Anti-Integrin alpha 5 antibody [EPR7854] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7854

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB, Flow Cyt (Intra), IP, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-low-endotoxin-azide-free-ab199376'>ab199376</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab221606 is the carrier-free version of ab150361.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

What does low endotoxin mean?
Our low endotoxin, azide-free formats have low endotoxin level (1 EU/mg, determined by the TAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Integrin alpha 5 also known as CD49e or ITGA5 is a subunit of the integrin family that forms heterodimers with beta 1 integrin. It has an approximate mass of 130 kDa. This protein mainly expresses in various tissues including fibroblasts endothelial cells and certain epithelial cells. Integrin alpha 5 mediates cell adhesion by binding to fibronectin an important extracellular matrix protein helping cells stay attached to their surrounding environment.
Biological function summary

Integrin alpha 5 plays a significant role in cellular processes such as migration proliferation and differentiation. It pairs with beta 1 integrin to form the alpha 5 beta 1 integrin complex. This complex facilitates the transmission of mechanical and chemical signals between the extracellular matrix and the cell interior. Integrin alpha 5 also interacts with intracellular proteins like talin and kindlin which are essential for initiating and strengthening the linkage to the actin cytoskeleton.

Pathways

Integrin alpha 5 is involved in the regulation of several critical signaling pathways. It prominently features within the MAPK/ERK pathway and the focal adhesion signaling pathway. Through these pathways integrin alpha 5 influences cell survival proliferation and migration. It interacts with proteins such as paxillin and focal adhesion kinase (FAK) within these pathways contributing to cellular responses to external stimuli.

Integrin alpha 5 is linked to conditions such as cancer and fibrosis. Overexpression or aberrant regulation of this integrin often associates with tumorigenesis particularly in breast and colorectal cancers enhancing cancer cell migration and invasion. Integrin alpha 5 is also connected to proteins like matrix metalloproteinases (MMPs) which play roles in extracellular matrix remodeling and are involved in fibrotic diseases. These associations highlight the importance of integrin alpha 5 in pathological processes and its potential as a target for therapeutic intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Integrin alpha-5/beta-1 (ITGA5 : ITGB1) is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. ITGA5 : ITGB1 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed : 18635536, PubMed : 25398877). ITGA5 : ITGB1 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1 (PubMed : 12807887, PubMed : 17158881). ITGA5 : ITGB1 acts as a receptor for fibronectin (FN1) and mediates R-G-D-dependent cell adhesion to FN1 (PubMed : 33962943). ITGA5 : ITGB1 is a receptor for IL1B and binding is essential for IL1B signaling (PubMed : 29030430). ITGA5 : ITGB3 is a receptor for soluble CD40LG and is required for CD40/CD40LG signaling (PubMed : 31331973).. (Microbial infection) Integrin ITGA5 : ITGB1 acts as a receptor for Human metapneumovirus.. (Microbial infection) Integrin ITGA2 : ITGB1 acts as a receptor for Human parvovirus B19.. (Microbial infection) In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
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Publications (3)

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Toxins 14: PubMed35050979

2021

Molecular Characterization and Functional Analysis of the Nattectin-like Toxin from the Venomous Fish .

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Monica Lopes-Ferreira,Ines Sosa-Rosales,Pedro Ismael Silva Junior,Katia Conceicao,Adolfo Luis Almeida Maleski,Leticia Balan-Lima,Geonildo Rodrigo Disner,Carla Lima

Frontiers in physiology 11:575598 PubMed33192578

2020

Increased Immunosignals of Collagen IV and Fibronectin Indicate Ischemic Consequences for the Neurovascular Matrix Adhesion Zone in Various Animal Models and Human Stroke Tissue.

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Dominik Michalski,Emma Spielvogel,Joana Puchta,Willi Reimann,Henryk Barthel,Björn Nitzsche,Bianca Mages,Carsten Jäger,Henrik Martens,Anja K E Horn,Stefan Schob,Wolfgang Härtig

The Journal of cell biology 214:389-99 PubMed27528657

2016

Atypical parkinsonism-associated retromer mutant alters endosomal sorting of specific cargo proteins.

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Kirsty J McMillan,Matthew Gallon,Adam P Jellett,Thomas Clairfeuille,Frances C Tilley,Ian McGough,Chris M Danson,Kate J Heesom,Kevin A Wilkinson,Brett M Collins,Peter J Cullen
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