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AB275977

Anti-Integrin alpha 5 + beta 1 antibody [M200 (Volociximab)] - Rabbit IgG (Chimeric)

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(5 Publications)

Rabbit Recombinant Monoclonal Integrin alpha 5 antibody. Suitable for Flow Cyt, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Protein within Human ITGA5.

View Alternative Names

CD49e, FNRA, ITGA5, Integrin alpha-5, CD49 antigen-like family member E, Fibronectin receptor subunit alpha, Integrin alpha-F, VLA-5, CD29, FNRB, MDF2, MSK12, ITGB1, Integrin beta-1, Fibronectin receptor subunit beta, Glycoprotein IIa, VLA-4 subunit beta, GPIIA

2 Images
Flow Cytometry - Anti-Integrin alpha 5 + beta 1 antibody [M200 (Volociximab)] - Rabbit IgG (Chimeric) (AB275977)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-Integrin alpha 5 + beta 1 antibody [M200 (Volociximab)] - Rabbit IgG (Chimeric) (AB275977)

PBMC's (A) and U93 cells (B) were stained with unimmunized rabbit IgG antibody (black line) or ab275977 (blue line) at a concentration of 10 μg/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody at 2 μg/ml and cells analyzed on a FACSCanto flow-cytometer.

Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha 5 + beta 1 antibody [M200 (Volociximab)] - Rabbit IgG (Chimeric) (AB275977)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha 5 + beta 1 antibody [M200 (Volociximab)] - Rabbit IgG (Chimeric) (AB275977)

Immunofluorescence analysis of paraformaldehyde fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells permeabilized with 0.15% Triton and labeled Integrin alpha 5 + beta 1 with ab275977 at 10 μg/ml for 1h followed by Alexa Fluor®488 secondary antibody (1 μg/ml), showing cytoplasmic and membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom ab275977, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor®488 secondary antibody.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

M200 (Volociximab)

Isotype

IgG

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

Flow Cyt, ICC/IF

applications

Immunogen

Recombinant Protein within Human ITGA5. The exact immunogen used to generate this antibody is proprietary information.

P08648

Specificity

The antibody binds to human α5β1 integrin with a Kd of 0.367 nM (determined by Biacore), an EC50 of 0.2 nM and an IC50 of 2.3 nM.

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Complementary Products

Primary Antibodies

AB30394

Anti-Integrin beta 1 antibody [12G10]

KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Integrin beta 1 antibody [12G10] (AB30394)

  • 5
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Primary Antibodies

AB179475

Anti-Integrin alpha V antibody [EPR16800]

KO Validated|RabMAb|Recombinant

Western blot - Anti-Integrin alpha V antibody [EPR16800] (AB179475)

  • 5
  • 10
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB268020

Anti-Fibronectin antibody [EPR23110-46]

KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [EPR23110-46] (AB268020)

  • 4
  • 5
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "10 µg/mL", "FlowCyt-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Product details

This rabbit monoclonal chimeric antibody was made using the variable domain sequences of the original Human IgG4 format, for improved compatibility with existing reagents, assays and techniques.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.02% Proclin 300 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Integrin alpha 5 beta 1 also known as alpha 5 beta 1 integrin is a cell surface receptor that facilitates cell adhesion and signaling. This integrin is a heterodimer composed of alpha 5 and beta 1 subunits each contributing to its structure and function. The molecular mass of integrin alpha 5 beta 1 is approximately 170 kDa. It predominantly expresses on the surface of fibroblasts endothelial cells and some epithelial cells where it plays an important role in mediating interactions between the cell and the extracellular matrix (ECM).
Biological function summary

Integrin alpha 5 beta 1 regulates processes like cell adhesion migration and differentiation. It binds to fibronectin in the ECM forming part of the adhesion complexes needed for cellular attachment and survival. This integrin couples with the actin cytoskeleton through talin and vinculin initiating signaling cascades that adjust cellular responses to changes in the microenvironment. Its mechanical linkage to the ECM helps sustain structural integrity affecting tissue homeostasis.

Pathways

Integrin alpha 5 beta 1 participates in the focal adhesion and the MAPK signaling pathway each with significant influence on cell behavior. In focal adhesion it partners with focal adhesion kinase (FAK) an important player in transmitting signals from the ECM to the intracellular environment. The MAPK pathway further involves integrin alpha 5 beta 1 integrating external signals to modulate gene expression and cell cycle progression. These pathways allow the integrin to coordinate cellular processes with the broader tissue context.

Integrin alpha 5 beta 1 associates with fibrotic diseases and cancer due to its role in cell adhesion and signaling. In fibrotic diseases such as pulmonary fibrosis altered expression and activity of integrin alpha 5 beta 1 can contribute to excessive ECM deposition and tissue stiffening. Similarly in cancer this integrin influences tumor progression and metastasis by modifying tumor microenvironment interactions. Proteins like fibronectin and transforming growth factor-beta (TGF-beta) intersect with integrin alpha 5 beta 1 activity impacting disease pathogenesis and progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Integrin alpha-5/beta-1 (ITGA5 : ITGB1) is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. ITGA5 : ITGB1 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed : 18635536, PubMed : 25398877). ITGA5 : ITGB1 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1 (PubMed : 12807887, PubMed : 17158881). ITGA5 : ITGB1 acts as a receptor for fibronectin (FN1) and mediates R-G-D-dependent cell adhesion to FN1 (PubMed : 33962943). ITGA5 : ITGB1 is a receptor for IL1B and binding is essential for IL1B signaling (PubMed : 29030430). ITGA5 : ITGB3 is a receptor for soluble CD40LG and is required for CD40/CD40LG signaling (PubMed : 31331973).. (Microbial infection) Integrin ITGA5 : ITGB1 acts as a receptor for Human metapneumovirus.. (Microbial infection) Integrin ITGA2 : ITGB1 acts as a receptor for Human parvovirus B19.. (Microbial infection) In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
See full target information ITGA5

Additional targets

ITGB1
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Cells 14: PubMed41002378

2025

ApoC3 Attenuates Platelet Activation Through GPIIb/IIIa Receptor Interaction.

Applications

Unspecified application

Species

Unspecified reactive species

Michael Holzer,Eva Gruden,Sanja Curcic,Gerhard Cvirn,Gunther Marsche

Acta pharmaceutica Sinica. B 15:692-706 PubMed40177561

2025

Enhanced radiotheranostic targeting of integrin 51 with PEGylation-enabled peptide multidisplay platform (PEGibody): A strategy for prolonged tumor retention with fast blood clearance.

Applications

Unspecified application

Species

Unspecified reactive species

Siqi Zhang,Xiaohui Ma,Jiang Wu,Jieting Shen,Yuntao Shi,Xingkai Wang,Lin Xie,Xiaona Sun,Yuxuan Wu,Hao Tian,Xin Gao,Xueyao Chen,Hongyi Huang,Lu Chen,Xuekai Song,Qichen Hu,Hailong Zhang,Feng Wang,Zhao-Hui Jin,Ming-Rong Zhang,Rui Wang,Kuan Hu

Stem cell reviews and reports 20:1060-1077 PubMed38418738

2024

Enhancing Vasculogenesis in Dental Pulp Development: DPSCs-ECs Communication via FN1-ITGA5 Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Tiankai Di,Chao Feng,Lulu Wang,Jinlong Xu,Yang Du,Baixiang Cheng,Yujiang Chen,Lian Wu

Methods in molecular biology (Clifton, N.J.) 2767:85-103 PubMed37402094

2023

Generation of 3D Trophoblast Organoids from Human Naïve Pluripotent Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Rowan M Karvas,Thorold W Theunissen

Cell stem cell 29:810-825.e8 PubMed35523141

2022

Stem-cell-derived trophoblast organoids model human placental development and susceptibility to emerging pathogens.

Applications

Unspecified application

Species

Unspecified reactive species

Rowan M Karvas,Shafqat A Khan,Sonam Verma,Yan Yin,Devesha Kulkarni,Chen Dong,Kyoung-Mi Park,Brian Chew,Eshan Sane,Laura A Fischer,Deepak Kumar,Liang Ma,Adrianus C M Boon,Sabine Dietmann,Indira U Mysorekar,Thorold W Theunissen
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
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