AB235905

Anti-Integrin alpha 6 antibody

Name: Anti-Integrin alpha 6 antibody (ab235905) | Abcam
Brand: Abcam Limited
SKU: AB235905
Price: 525 USD
Availability: InStock

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(9 Publications)

Rabbit Polyclonal Integrin alpha 6 antibody. Suitable for WB, ICC/IF and reacts with Rat, Mouse, Human samples. Cited in 9 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ITGA6 aa 600-900.

View Alternative Names

CD49f, Integrin alpha-6, CD49 antigen-like family member F, VLA-6, ITGA6

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha 6 antibody (AB235905)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha 6 antibody (AB235905)

4% formaldehyde-fixed, 0.2% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells laneling Integrin alpha 6 using ab235905 at 1/200 dilution in ICC/IF. Cells were blocked in 10% normal goat serum and incubated with primary antibody overnight at 4°C. Secondary antibody was Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (H+L).

Supplier Data

Western blot - Anti-Integrin alpha 6 antibody (AB235905)

All lanes:

Western blot - Anti-Integrin alpha 6 antibody (ab235905) at 1/500 dilution

Lane 1:

Rat heart lysate

Lane 2:

Rat kidney lysate

Lane 3:

Mouse heart lysate

Lane 4:

Mouse kidney lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 126 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human ITGA6 aa 600-900. The exact immunogen used to generate this antibody is proprietary information.

P23229

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200 - 1/500", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purity >95%.

Storage buffer

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Integrin alpha 6 also known as ITGA6 or CD49f functions as a transmembrane receptor protein involved in cell adhesion and signal transduction. As part of the broader integrin family integrin alpha 6 typically pairs with the beta 1 or beta 4 subunits forming the alpha6beta1 or alpha6beta4 integrins respectively. It has a molecular mass of approximately 150 kDa. This protein is mainly expressed in epithelial cells but also found in platelets certain immune cells and in the nervous system. Its expression patterns highlight its role in a variety of cellular and tissue functions.

Biological function summary

The integrin alpha 6 protein partners with the beta 4 subunit to form a heterodimer complex that plays an important role in cellular adhesion to the extracellular matrix specifically interacting with laminins. Its main action helps cells attach to the basement membrane which is an important component of epithelial tissue structure and integrity. The alpha 6 integrin provides structural cell support and participates in transmitting signals that influence cell behavior such as movement survival and proliferation.

Pathways

Integrins including the alpha 6 variant are important components in the integrin signaling pathway and are involved in cell motility and survival pathways. In particular the alpha 6 integrin partners with proteins like focal adhesion kinase (FAK) and phosphoinositide 3-kinase (PI3K) to initiate downstream signaling cascades. The interaction with laminins activates pathways critical for developmental processes and tissue remodeling highlighting their role in maintaining cellular environments.

Integrin alpha 6 has significant implications in cancer progression and skin blistering disorders. In various cancers such as breast and skin cancers upregulation of integrin alpha 6 promotes tumor growth and metastasis through interaction with proteins like collagen and laminins found in the tumor microenvironment. Furthermore in the context of blistering disorders such as epidermolysis bullosa mutations in the beta 4 subunit that pairs with alpha 6 can disrupt the integrity of epithelial tissues leading to severe impact on the skin's structural stability.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Integrin alpha-6/beta-1 (ITGA6 : ITGB1) is a receptor for laminin on platelets (By similarity). Integrin alpha-6/beta-1 (ITGA6 : ITGB1) is present in oocytes and is involved in sperm-egg fusion (By similarity). Integrin alpha-6/beta-4 (ITGA6 : ITGB4) is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome (By similarity). ITGA6 : ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed : 20682778). ITGA6 : ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed : 22351760). ITGA6 : ITGB4 binds to IGF2 and this binding is essential for IGF2 signaling (PubMed : 28873464).

See full target information ITGA6

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Breast cancer research : BCR 26:187 PubMed39707454

2024

Cilengitide sensitivity is predicted by overall integrin expression in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Nomeda Girnius,Aylin Z Henstridge,Benjamin Marks,Jeffrey K Yu,G Kenneth Gray,Chris Sander,Ioannis K Zervantonakis,Augustin Luna

Frontiers in immunology 15:1394530 PubMed38881903

2024

Poly-l-lactic acid microspheres delay aging of epidermal stem cells in rat skin.

Applications

Unspecified application

Species

Unspecified reactive species

Yunxian Dong,Youliang Zhang,Hao Yu,Lingcong Zhou,Yaan Zhang,Haibin Wang,Zhicheng Hu,Shengkang Luo

Stem cell research & therapy 14:51 PubMed36959609

2023

Single-stage transplantation combined with epidermal stem cells promotes the survival of tissue-engineered skin by inducing early angiogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiyong Wang,Hailin Xu,Hao Yang,Yi Zhang,Xiaoyan Wang,Peng Wang,Zhongye Xu,Dongming Lv,Yanchao Rong,Yunxian Dong,Bing Tang,Zhicheng Hu,Wuguo Deng,Jiayuan Zhu

Cell reports 41:111580 PubMed36323248

2022

Multi-omic profiling reveals the ataxia protein sacsin is required for integrin trafficking and synaptic organization.

Applications

Unspecified application

Species

Unspecified reactive species

Lisa E L Romano,Wen Yih Aw,Kathryn M Hixson,Tatiana V Novoselova,Tammy M Havener,Stefanie Howell,Bonnie Taylor-Blake,Charlotte L Hall,Lei Xing,Josh Beri,Suran Nethisinghe,Laura Perna,Abubakar Hatimy,Ginevra Chioccioli Altadonna,Lee M Graves,Laura E Herring,Anthony J Hickey,Konstantinos Thalassinos,J Paul Chapple,Justin M Wolter

Stem cell research & therapy 13:346 PubMed35883101

2022

Melatonin in cryopreservation media improves transplantation efficiency of frozen-thawed spermatogonial stem cells into testes of azoospermic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Shokoofeh Kazemzadeh,Shahram Mohammadpour,Soheila Madadi,Azar Babakhani,Maryam Shabani,Maryam Khanehzad

Upsala journal of medical sciences 126: PubMed34471485

2021

LncRNA DRAIC regulates cell proliferation and migration by affecting the miR-34a-5p/ITGA6 signal axis in Hirschsprung's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Chuancheng Sun,Bing Xu,Liang Wang,Yilin Su

Cancer cell international 20:574 PubMed33317527

2020

Exosomal miR-126 blocks the development of non-small cell lung cancer through the inhibition of ITGA6.

Applications

Unspecified application

Species

Unspecified reactive species

Mingjun Li,Qianqian Wang,Xiaofei Zhang,Ningning Yan,Xingya Li

Stem cell research & therapy 11:344 PubMed32771044

2020

Rat epidermal stem cells promote the angiogenesis of full-thickness wounds.

Applications

Unspecified application

Species

Unspecified reactive species

Shaobin Huang,Zhicheng Hu,Peng Wang,Yi Zhang,Xiaoling Cao,Yunxian Dong,Pu Cheng,Hailin Xu,Wenkai Zhu,Bing Tang,Jiayuan Zhu

International journal of molecular medicine 43:517-524 PubMed30365062

2018

miR-186 reverses cisplatin resistance and inhibits the formation of the glioblastoma-initiating cell phenotype by degrading Yin Yang 1 in glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Li,Jie Song,Feng Guo

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Integrin alpha 6 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha 6 antibody (AB235905)

Western blot - Anti-Integrin alpha 6 antibody (AB235905)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

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