AB97760

Anti-Integrin alpha 6 antibody

Name: Anti-Integrin alpha 6 antibody (ab97760) | Abcam
Brand: Abcam Limited
SKU: AB97760
Price: 565 USD
Availability: InStock
Rating: 5 (2 reviews)

(2 Reviews)

(10 Publications)

Rabbit Polyclonal Integrin alpha 6 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 10 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ITGA6 aa 500-850.

View Alternative Names

CD49f, Integrin alpha-6, CD49 antigen-like family member F, VLA-6, ITGA6

2 Images

Supplier Data

Western blot - Anti-Integrin alpha 6 antibody (AB97760)

Samples were separated by 5% SDS-PAGE.

All lanes:

Western blot - Anti-Integrin alpha 6 antibody (ab97760) at 1/2000 dilution

All lanes:

Human ESC whole cell lysate at 20 µg

Secondary

All lanes:

HRP conjugated anti rabbit IgG antibody

Predicted band size: 120 kDa

false

Supplier Data

Western blot - Anti-Integrin alpha 6 antibody (AB97760)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-Integrin alpha 6 antibody (ab97760) at 1/500 dilution

All lanes:

Mouse lung tissue lysates at 50 µg

Secondary

All lanes:

HRP conjugated anti rabbit IgG antibody

Predicted band size: 120 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

applications

Immunogen

Recombinant Fragment Protein within Human ITGA6 aa 500-850. The exact immunogen used to generate this antibody is proprietary information.

P23229

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Integrin alpha 6 also known as ITGA6 or CD49f functions as a transmembrane receptor protein involved in cell adhesion and signal transduction. As part of the broader integrin family integrin alpha 6 typically pairs with the beta 1 or beta 4 subunits forming the alpha6beta1 or alpha6beta4 integrins respectively. It has a molecular mass of approximately 150 kDa. This protein is mainly expressed in epithelial cells but also found in platelets certain immune cells and in the nervous system. Its expression patterns highlight its role in a variety of cellular and tissue functions.

Biological function summary

The integrin alpha 6 protein partners with the beta 4 subunit to form a heterodimer complex that plays an important role in cellular adhesion to the extracellular matrix specifically interacting with laminins. Its main action helps cells attach to the basement membrane which is an important component of epithelial tissue structure and integrity. The alpha 6 integrin provides structural cell support and participates in transmitting signals that influence cell behavior such as movement survival and proliferation.

Pathways

Integrins including the alpha 6 variant are important components in the integrin signaling pathway and are involved in cell motility and survival pathways. In particular the alpha 6 integrin partners with proteins like focal adhesion kinase (FAK) and phosphoinositide 3-kinase (PI3K) to initiate downstream signaling cascades. The interaction with laminins activates pathways critical for developmental processes and tissue remodeling highlighting their role in maintaining cellular environments.

Integrin alpha 6 has significant implications in cancer progression and skin blistering disorders. In various cancers such as breast and skin cancers upregulation of integrin alpha 6 promotes tumor growth and metastasis through interaction with proteins like collagen and laminins found in the tumor microenvironment. Furthermore in the context of blistering disorders such as epidermolysis bullosa mutations in the beta 4 subunit that pairs with alpha 6 can disrupt the integrity of epithelial tissues leading to severe impact on the skin’s structural stability.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Integrin alpha-6/beta-1 (ITGA6 : ITGB1) is a receptor for laminin on platelets (By similarity). Integrin alpha-6/beta-1 (ITGA6 : ITGB1) is present in oocytes and is involved in sperm-egg fusion (By similarity). Integrin alpha-6/beta-4 (ITGA6 : ITGB4) is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome (By similarity). ITGA6 : ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed : 20682778). ITGA6 : ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed : 22351760). ITGA6 : ITGB4 binds to IGF2 and this binding is essential for IGF2 signaling (PubMed : 28873464).

See full target information ITGA6

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

The Journal of cell biology 220: PubMed34550317

2021

EGFR-mediated epidermal stem cell motility drives skin regeneration through COL17A1 proteolysis.

Applications

Unspecified application

Species

Unspecified reactive species

Daisuke Nanba,Fujio Toki,Kyosuke Asakawa,Hiroyuki Matsumura,Ken Shiraishi,Koji Sayama,Kyoichi Matsuzaki,Hiroshi Toki,Emi K Nishimura

OncoTargets and therapy 13:7399-7409 PubMed32801763

2020

FKBP10 Acts as a New Biomarker for Prognosis and Lymph Node Metastasis of Gastric Cancer by Bioinformatics Analysis and in Vitro Experiments.

Applications

Unspecified application

Species

Unspecified reactive species

Li-Bao Gong,Chuang Zhang,Ruo-Xi Yu,Ce Li,Yi-Bo Fan,Yun-Peng Liu,Xiu-Juan Qu

American journal of cancer research 10:1026-1044 PubMed32266108

2020

LPPR4 promotes peritoneal metastasis via Sp1/integrin α/FAK signaling in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Zang,Chuang Zhang,Ce Li,Yibo Fan,Zhi Li,Kezuo Hou,Xiaofang Che,Yunpeng Liu,Xiujuan Qu

International journal of biological sciences 16:1044-1058 PubMed32140072

2020

Functional Passenger-Strand miRNAs in Exosomes Derived from Human Colon Cancer Cells and Their Heterogeneous Paracrine Effects.

Applications

Unspecified application

Species

Unspecified reactive species

Qingkun Gao,Fuming Lei,Qingmin Zeng,Zhaoya Gao,Pengfei Niu, Junnan, Ning,Jie Li,Jun Zhang

Stem cell research & therapy 8:76 PubMed28412969

2017

Engagement of cellular prion protein with the co-chaperone Hsp70/90 organizing protein regulates the proliferation of glioblastoma stem-like cells.

Applications

Unspecified application

Species

Unspecified reactive species

Rebeca Piatniczka Iglesia,Mariana Brandão Prado,Lilian Cruz,Vilma Regina Martins,Tiago Góss Santos,Marilene Hohmuth Lopes

Cell 166:1295-1307.e21 PubMed27565350

2016

Proteomic Analysis of Unbounded Cellular Compartments: Synaptic Clefts.

Applications

Unspecified application

Species

Unspecified reactive species

Ken H Loh,Philipp S Stawski,Austin S Draycott,Namrata D Udeshi,Emily K Lehrman,Daniel K Wilton,Tanya Svinkina,Thomas J Deerinck,Mark H Ellisman,Beth Stevens,Steven A Carr,Alice Y Ting

In vitro cellular & developmental biology. Animal 51:287-99 PubMed25319126

2014

Up-regulation of integrin α6β4 expression by mitogens involved in dairy cow mammary development.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Zhao,Chang Liu,Yu-Meng Hao,Bo Qu,Ying-Jun Cui,Na Zhang,Xue-Jun Gao,Qing-Zhang Li

Molecular biology of the cell 25:1560-73 PubMed24623721

2014

The membrane scaffold CD82 regulates cell adhesion by altering α4 integrin stability and molecular density.

Applications

Unspecified application

Species

Unspecified reactive species

Christina M Termini,Maura L Cotter,Kristopher D Marjon,Tione Buranda,Keith A Lidke,Jennifer M Gillette

British journal of cancer 109:2636-45 PubMed24091622

2013

Tumour-suppressive microRNA-29s inhibit cancer cell migration and invasion by targeting laminin-integrin signalling in head and neck squamous cell carcinoma.

Applications

Species

Human

T Kinoshita,N Nohata,T Hanazawa,N Kikkawa,N Yamamoto,H Yoshino,T Itesako,H Enokida,M Nakagawa,Y Okamoto,N Seki

Molecular biology of the cell 23:121-36 PubMed22031290

2011

Laminin 511 partners with laminin 332 to mediate directional migration of Madin-Darby canine kidney epithelial cells.

Applications

Species

Dog

Patricia G Greciano,Jose V Moyano,Mary M Buschmann,Jun Tang,Yue Lu,Jean Rudnicki,Aki Manninen,Karl S Matlin

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Integrin alpha 6 antibody

Western blot - Anti-Integrin alpha 6 antibody (AB97760)

Western blot - Anti-Integrin alpha 6 antibody (AB97760)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (10)

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