Anti-Integrin alpha 6 antibody [EPR18124]
- RabMAb
- Recombinant
- KO Validated
- What is this?
4
(6 Reviews)
|
(56 Publications)
Anti-Integrin alpha 6 antibody [EPR18124] (ab181551) is a rabbit monoclonal antibody detecting Integrin alpha 6 in Western Blot, IHC-P, IHC-Fr. Suitable for Human, Mouse, Rat.
- KO validated for confirmed specificity
- Biophysical QC for unrivalled batch-batch consistency
- Over 30 publications
View Alternative Names
CD49f, Integrin alpha-6, CD49 antigen-like family member F, VLA-6, ITGA6
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 6 antibody [EPR18124] (AB181551)
Immunohistochemical analysis of paraffin-embedded Human endometrial cancer tissue labeling Intergrin alpha 6 with ab181551 at 1/500 followed by Goat Anti-Rabbit IgG (HRP) at 1/500.
Membrane and cytoplasm staining on tumor cells of Human endometrial cancer tissue is observed.
Counter stained with Hematoxylin.
Negative control : Used PBS instead of primary antibody followed by Goat Anti-Rabbit IgG (HRP) at 1/500.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 6 antibody [EPR18124] (AB181551)
Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling Intergrin alpha 6 with ab181551 at 1/500 followed by Goat Anti-Rabbit IgG (HRP) at 1/500.
Membrane and cytoplasm staining on epithelial cells of Human breast tissue is observed.
Counter stained with Hematoxylin.
Negative control : Used PBS instead of primary antibody followed by Goat Anti-Rabbit IgG (HRP) at 1/500.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- IHC-Fr
Unknown
Immunohistochemistry (Frozen sections) - Anti-Integrin alpha 6 antibody [EPR18124] (AB181551)
Immunohistochemistry (Frozen sections) analysis of mouse stomach tissue (4% PFA, 0.2% Tritron x-100) labelling Integrin alpha 6 with ab240252 at 1/100 dilution. ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1000 dilution. Negative control using PBS instead of primary antibody (left). Counterstained with DAPI.
Positive staining was seen on mouse stomach.
- IHC-Fr
Unknown
Immunohistochemistry (Frozen sections) - Anti-Integrin alpha 6 antibody [EPR18124] (AB181551)
Immunohistochemistry (Frozen sections) analysis of rat stomach tissue (4% PFA, 0.2% Tritron x-100) labelling Integrin alpha 6 with ab240252 at 1/100 dilution. ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1000 dilution. Negative control using PBS instead of primary antibody (left). Counterstained with DAPI.
Positive staining was seen on rat stomach.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 6 antibody [EPR18124] (AB181551)
Immunohistochemical analysis of paraffin-embedded Rat stomach tissue labeling Intergrin alpha 6 with ab181551 at 1/500 followed by Goat Anti-Rabbit IgG (HRP) at 1/500.
Basement membrane staining on tumor cells of Rat stomach tissue is observed.
Counter stained with Hematoxylin.
Negative control : Used PBS instead of primary antibody followed by Goat Anti-Rabbit IgG (HRP) at 1/500.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 6 antibody [EPR18124] (AB181551)
Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling Intergrin alpha 6 with ab181551 at 1/500 followed by Goat Anti-Rabbit IgG (HRP) at 1/500.
Basement membrane staining on tumor cells of Mouse stomach tissue is observed.
Counter stained with Hematoxylin.
Negative control : Used PBS instead of primary antibody followed by Goat Anti-Rabbit IgG (HRP) at 1/500.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- WB
Lab
Western blot - Anti-Integrin alpha 6 antibody [EPR18124] (AB181551)
Lanes 1 - 3 : Merged signal (red and green). Green - ab181551 observed at 130 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab181551 was shown to react with Integrin alpha 6 in wild-type SW480 cells in western blot with loss of signal observed in ITGA6 knockout cell line ab265838 (knockout cell lysate ab256957). Wild-type and ITGA6 knockout SW480 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab181551 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 2000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-Integrin alpha 6 antibody [EPR18124] (ab181551) at 1/2000 dilution
Lane 1:
Wild-type SW480 cell lysate at 20 µg
Lane 2:
ITGA6 knockout SW480 cell lysate at 20 µg
Lane 3:
HEK-293 cell lysate at 20 µg
Secondary
All lanes:
Goat anti-Rabbit IgG H&L preabsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) and Goat anti-Mouse IgG H&L preabsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-irdye-680rd-preadsorbed-ab216776'>ab216776</a>) at 1/20000 dilution
Predicted band size: 126 kDa
Observed band size: 130 kDa
false
- WB
Supplier Data
Western blot - Anti-Integrin alpha 6 antibody [EPR18124] (AB181551)
Blocking and diluting buffer was 5% NFDM /TBST.
All lanes:
Western blot - Anti-Integrin alpha 6 antibody [EPR18124] (ab181551) at 1/10000 dilution
Lane 1:
SW480 (Human colon adenocarcinoma cell line) whole cell lysate at 20 µg
Lane 2:
A549 (Human lung carcinoma) whole cell lysate at 20 µg
Lane 3:
Mouse skin lysate at 20 µg
Lane 4:
Rat skin lysate at 20 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 126 kDa
Observed band size: 127 kDa
true
Exposure time: 3min
- WB
Supplier Data
Western blot - Anti-Integrin alpha 6 antibody [EPR18124] (AB181551)
Blocking and diluting buffer was 5% NFDM /TBST.
All lanes:
Western blot - Anti-Integrin alpha 6 antibody [EPR18124] (ab181551) at 1/2000 dilution
All lanes:
Human fetal kidney tissue lysate at 10 µg
Secondary
All lanes:
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 126 kDa
Observed band size: 127 kDa
true
Exposure time: 3min
- WB
Lab
Western blot - Anti-Integrin alpha 6 antibody [EPR18124] (AB181551)
Lanes 1 - 3 : Merged signal (red and green). Green - ab181551 observed at 130 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab181551 was shown to react with Integrin alpha 6 in wild-type SW480 cells in western blot with loss of signal observed in ITGA6 knockout cell line ab265838 (knockout cell lysate ab256957). Wild-type and ITGA6 knockout SW480 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab181551 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 2000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-Integrin alpha 6 antibody [EPR18124] (ab181551) at 1/2000 dilution
Lane 1:
Wild-type SW480 cell lysate at 20 µg
Lane 2:
ITGA6 knockout SW480 cell lysate at 20 µg
Lane 2:
Western blot - Human ITGA6 (Integrin alpha 6) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-itga6-integrin-alpha-6-knockout-hela-cell-line-ab265838'>ab265838</a>)
Lane 3:
HEK-293 cell lysate at 20 µg
Predicted band size: 126 kDa,127 kDa
Observed band size: 130 kDa
false
- WB
Supplier Data
Western blot - Anti-Integrin alpha 6 antibody [EPR18124] (AB181551)
Blocking and diluting buffer was 5% NFDM /TBST
All lanes:
Western blot - Anti-Integrin alpha 6 antibody [EPR18124] (ab181551) at 1/10000 dilution
Lane 1:
F9 (mouse embryo testicular cancer cell line) whole cell lysate at 10 µg
Lane 2:
Rat spleen tissue lysate at 10 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 126 kDa
Observed band size: 127 kDa
true
Exposure time: 3min
- WB
Supplier Data
Western blot - Anti-Integrin alpha 6 antibody [EPR18124] (AB181551)
Blocking and diluting buffer was 5% NFDM /TBST
All lanes:
Western blot - Anti-Integrin alpha 6 antibody [EPR18124] (ab181551) at 1/2000 dilution
Lane 1:
Mouse kidney tissue lysate at 10 µg
Lane 2:
Mouse spleen tissue lysate at 10 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 126 kDa
Observed band size: 127 kDa
true
Exposure time: 10s
Related conjugates and formulations (7)
-
Anti-Integrin alpha 6 antibody [EPR18124] - BSA and Azide free
-
603 Alexa Fluor® 568
Alexa Fluor® 568 Anti-Integrin alpha 6 antibody [EPR18124]
-
617 Alexa Fluor® 594
Alexa Fluor® 594 Anti-Integrin alpha 6 antibody [EPR18124]
-
665 Alexa Fluor® 647
Alexa Fluor® 647 Anti-Integrin alpha 6 antibody [EPR18124]
-
519 Alexa Fluor® 488
Alexa Fluor® 488 Anti-Integrin alpha 6 antibody [EPR18124]
-
565 Alexa Fluor® 555
Alexa Fluor® 555 Anti-Integrin alpha 6 antibody [EPR18124]
-
775 Alexa Fluor® 750
Alexa Fluor® 750 Anti-Integrin alpha 6 antibody [EPR18124]
Reactivity data
Product details
What is this antibody validated in?
Anti-Integrin alpha 6 antibody [EPR18124] (ab181551) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr) in Human, Mouse, Rat samples.
What is the molecular weight of Integrin alpha 6?
Anti-Integrin alpha 6 [EPR18124] (ab181551) specifically detects a band for Integrin alpha 6 (UniProt: P23229) at a molecular weight of 127kDa.
Trusted by the scientific community
Anti-Integrin alpha 6 [EPR18124] (ab181551) was first used in a scientific publication in 2015 and has been cited over 30 times in peer-reviewed journals.
Reviewed by scientists
Anti-Integrin alpha 6 [EPR18124] (ab181551) has over 5 independent reviews from customers.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.
Specificity confirmed
The specificity of Anti-Integrin alpha 6 antibody [EPR18124] (ab181551) has been confirmed by Western blot testing in ITGA6 Knockout SW480 cell line, ab265838.
Other related products
We have a range of other formats of antibody clone [EPR18124] also available for your convenience: ab181551, Carrier free - ab240252, Alexa Fluor® 488 - ab311018, Alexa Fluor® 647 - ab311146, Alexa Fluor® 594 - ab311787, Alexa Fluor® 568 - ab313068, Alexa Fluor® 555 - ab313265, Alexa Fluor® 750 - ab321254
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The integrin alpha 6 protein partners with the beta 4 subunit to form a heterodimer complex that plays an important role in cellular adhesion to the extracellular matrix specifically interacting with laminins. Its main action helps cells attach to the basement membrane which is an important component of epithelial tissue structure and integrity. The alpha 6 integrin provides structural cell support and participates in transmitting signals that influence cell behavior such as movement survival and proliferation.
Pathways
Integrins including the alpha 6 variant are important components in the integrin signaling pathway and are involved in cell motility and survival pathways. In particular the alpha 6 integrin partners with proteins like focal adhesion kinase (FAK) and phosphoinositide 3-kinase (PI3K) to initiate downstream signaling cascades. The interaction with laminins activates pathways critical for developmental processes and tissue remodeling highlighting their role in maintaining cellular environments.
Product protocols
- Visit the General protocols
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Target data
Publications (56)
Recent publications for all applications. Explore the full list and refine your search
Nature communications 16:7287 PubMed40775187
2025
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Cancer research communications 5:1319-1331 PubMed40728119
2025
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Molecular medicine reports 31: PubMed39981899
2025
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The Journal of cell biology 223: PubMed38551497
2024
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Cancer research 84:1781-1798 PubMed38507720
2024
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Journal of translational medicine 22:288 PubMed38493128
2024
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Biomaterials advances 159:213825 PubMed38479242
2024
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Regenerative therapy 25:113-127 PubMed38226057
2024
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Journal of experimental & clinical cancer research : CR 42:328 PubMed38031171
2023
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Molecular cancer research : MCR 22:169-180 PubMed37878345
2023
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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