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Rabbit Recombinant Monoclonal Integrin alpha 6 antibody. Carrier free. Suitable for WB, IHC-Fr, IHC-P and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-Integrin alpha 6 antibody [EPR18124] - BSA and Azide free (AB240252), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-Integrin alpha 6 antibody [EPR18124] - BSA and Azide free (AB240252), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 6 antibody [EPR18124] - BSA and Azide free (AB240252), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 6 antibody [EPR18124] - BSA and Azide free (AB240252), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 6 antibody [EPR18124] - BSA and Azide free (AB240252), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-FrIHC-P
Human
Tested
Expected
Expected
Mouse
Expected
Tested
Tested
Rat
Expected
Tested
Expected

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/100
Notes

-

Species
Rat
Dilution info
1/100
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Human, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

9 products for Alternative Product

2 products for Alternative Version

Target data

Function

Integrin alpha-6/beta-1 (ITGA6:ITGB1) is a receptor for laminin on platelets (By similarity). Integrin alpha-6/beta-1 (ITGA6:ITGB1) is present in oocytes and is involved in sperm-egg fusion (By similarity). Integrin alpha-6/beta-4 (ITGA6:ITGB4) is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome (By similarity). ITGA6:ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed:20682778). ITGA6:ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed:22351760). ITGA6:ITGB4 binds to IGF2 and this binding is essential for IGF2 signaling (PubMed:28873464).

Alternative names

CD49f, Integrin alpha-6, CD49 antigen-like family member F, VLA-6, ITGA6

Recommended products

Rabbit Recombinant Monoclonal Integrin alpha 6 antibody. Carrier free. Suitable for WB, IHC-Fr, IHC-P and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR18124
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab240252 is the carrier-free version of Anti-Integrin alpha 6 antibody [EPR18124] ab181551.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Integrin alpha 6 also known as ITGA6 or CD49f functions as a transmembrane receptor protein involved in cell adhesion and signal transduction. As part of the broader integrin family integrin alpha 6 typically pairs with the beta 1 or beta 4 subunits forming the alpha6beta1 or alpha6beta4 integrins respectively. It has a molecular mass of approximately 150 kDa. This protein is mainly expressed in epithelial cells but also found in platelets certain immune cells and in the nervous system. Its expression patterns highlight its role in a variety of cellular and tissue functions.

Biological function summary

The integrin alpha 6 protein partners with the beta 4 subunit to form a heterodimer complex that plays an important role in cellular adhesion to the extracellular matrix specifically interacting with laminins. Its main action helps cells attach to the basement membrane which is an important component of epithelial tissue structure and integrity. The alpha 6 integrin provides structural cell support and participates in transmitting signals that influence cell behavior such as movement survival and proliferation.

Pathways

Integrins including the alpha 6 variant are important components in the integrin signaling pathway and are involved in cell motility and survival pathways. In particular the alpha 6 integrin partners with proteins like focal adhesion kinase (FAK) and phosphoinositide 3-kinase (PI3K) to initiate downstream signaling cascades. The interaction with laminins activates pathways critical for developmental processes and tissue remodeling highlighting their role in maintaining cellular environments.

Associated diseases and disorders

Integrin alpha 6 has significant implications in cancer progression and skin blistering disorders. In various cancers such as breast and skin cancers upregulation of integrin alpha 6 promotes tumor growth and metastasis through interaction with proteins like collagen and laminins found in the tumor microenvironment. Furthermore in the context of blistering disorders such as epidermolysis bullosa mutations in the beta 4 subunit that pairs with alpha 6 can disrupt the integrity of epithelial tissues leading to severe impact on the skin’s structural stability.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-Integrin alpha 6 antibody [EPR18124] - BSA and Azide free (ab240252), expandable thumbnail

    Western blot - Anti-Integrin alpha 6 antibody [EPR18124] - BSA and Azide free (ab240252)

    This data was developed using the same antibody clone in a different buffer formulation (Anti-Integrin alpha 6 antibody [EPR18124] ab181551).

    Lanes 1 - 3: Merged signal (red and green). Green - Anti-Integrin alpha 6 antibody [EPR18124] ab181551 observed at 130 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

    Anti-Integrin alpha 6 antibody [EPR18124] ab181551 was shown to react with Integrin alpha 6 in wild-type SW480 cells in western blot with loss of signal observed in ITGA6 knockout sample. Wild-type and ITGA6 knockout SW480 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with Anti-Integrin alpha 6 antibody [EPR18124] ab181551 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 2000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Integrin alpha 6 antibody [EPR18124] (Anti-Integrin alpha 6 antibody [EPR18124] ab181551) at 1/2000 dilution

    Lane 1: Wild-type SW480 cell lysate at 20 µg

    Lane 2: ITGA6 knockout SW480 cell lysate at 20 µg

    Lane 2: Western blot - Human ITGA6 (Integrin alpha 6) knockout HeLa cell line (Human ITGA6 (Integrin alpha 6) knockout HeLa cell line ab265838)

    Lane 3: HEK-293 cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 126 kDa, 127 kDa

    Observed band size: 130 kDa

  • Immunohistochemistry (Frozen sections) - Anti-Integrin alpha 6 antibody [EPR18124] - BSA and Azide free (ab240252), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Integrin alpha 6 antibody [EPR18124] - BSA and Azide free (ab240252)

    This data was developed using the same antibody clone in a different buffer formulation - Anti-Integrin alpha 6 antibody [EPR18124] ab181551.

    Immunohistochemistry (Frozen sections) analysis of rat stomach tissue (4% PFA, 0.2% Tritron x-100) labelling Integrin alpha 6 with ab240252 at 1/100 dilution. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1000 dilution. Negative control using PBS instead of primary antibody (left). Counterstained with DAPI.

    Positive staining was seen on rat stomach.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 6 antibody [EPR18124] - BSA and Azide free (ab240252), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 6 antibody [EPR18124] - BSA and Azide free (ab240252)

    Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling Intergrin alpha 6 with Anti-Integrin alpha 6 antibody [EPR18124] ab181551 at 1/500 followed by Goat Anti-Rabbit IgG (HRP) at 1/500.

    Membrane and cytoplasm staining on epithelial cells of Human breast tissue is observed.

    Counter stained with Hematoxylin.
    Negative control: Used PBS instead of primary antibody followed by Goat Anti-Rabbit IgG (HRP) at 1/500.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Integrin alpha 6 antibody [EPR18124] ab181551).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 6 antibody [EPR18124] - BSA and Azide free (ab240252), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 6 antibody [EPR18124] - BSA and Azide free (ab240252)

    Immunohistochemical analysis of paraffin-embedded Human endometrial cancer tissue labeling Intergrin alpha 6 with Anti-Integrin alpha 6 antibody [EPR18124] ab181551 at 1/500 followed by Goat Anti-Rabbit IgG (HRP) at 1/500.

    Membrane and cytoplasm staining on tumor cells of Human endometrial cancer tissue is observed.

    Counter stained with Hematoxylin.
    Negative control: Used PBS instead of primary antibody followed by Goat Anti-Rabbit IgG (HRP) at 1/500.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Integrin alpha 6 antibody [EPR18124] ab181551).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 6 antibody [EPR18124] - BSA and Azide free (ab240252), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 6 antibody [EPR18124] - BSA and Azide free (ab240252)

    Immunohistochemical analysis of paraffin-embedded Rat stomach tissue labeling Intergrin alpha 6 with Anti-Integrin alpha 6 antibody [EPR18124] ab181551 at 1/500 followed by Goat Anti-Rabbit IgG (HRP) at 1/500.

    Basement membrane staining on tumor cells of Rat stomach tissue is observed.

    Counter stained with Hematoxylin.
    Negative control: Used PBS instead of primary antibody followed by Goat Anti-Rabbit IgG (HRP) at 1/500.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Integrin alpha 6 antibody [EPR18124] ab181551).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Frozen sections) - Anti-Integrin alpha 6 antibody [EPR18124] - BSA and Azide free (ab240252), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Integrin alpha 6 antibody [EPR18124] - BSA and Azide free (ab240252)

    This data was developed using the same antibody clone in a different buffer formulation - Anti-Integrin alpha 6 antibody [EPR18124] ab181551.

    Immunohistochemistry (Frozen sections) analysis of mouse stomach tissue (4% PFA, 0.2% Tritron x-100) labelling Integrin alpha 6 with ab240252 at 1/100 dilution. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1000 dilution. Negative control using PBS instead of primary antibody (left). Counterstained with DAPI.

    Positive staining was seen on mouse stomach.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 6 antibody [EPR18124] - BSA and Azide free (ab240252), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 6 antibody [EPR18124] - BSA and Azide free (ab240252)

    Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling Intergrin alpha 6 with Anti-Integrin alpha 6 antibody [EPR18124] ab181551 at 1/500 followed by Goat Anti-Rabbit IgG (HRP) at 1/500.

    Basement membrane staining on tumor cells of Mouse stomach tissue is observed.

    Counter stained with Hematoxylin.
    Negative control: Used PBS instead of primary antibody followed by Goat Anti-Rabbit IgG (HRP) at 1/500.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Integrin alpha 6 antibody [EPR18124] ab181551).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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