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Rabbit Recombinant Monoclonal Integrin alpha 6 antibody. Suitable for WB and reacts with Human samples. Cited in 5 publications.


Images

Western blot - Anti-Integrin alpha 6 antibody [EPR5578] (AB124924), expandable thumbnail
  • Western blot - Anti-Integrin alpha 6 antibody [EPR5578] (AB124924), expandable thumbnail
  • Western blot - Anti-Integrin alpha 6 antibody [EPR5578] (AB124924), expandable thumbnail
  • Western blot - Anti-Integrin alpha 6 antibody [EPR5578] (AB124924), expandable thumbnail
  • OI-RD Scanning - Anti-Integrin alpha 6 antibody [EPR5578] (AB124924), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFFlow CytWBIHC-P
Human
Not recommended
Not recommended
Tested
Not recommended

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

Heat up to 98°C, below boiling, and then let cool for 10-20 min.

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Associated Products

Select an associated product type

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Target data

Function

Integrin alpha-6/beta-1 (ITGA6:ITGB1) is a receptor for laminin on platelets (By similarity). Integrin alpha-6/beta-1 (ITGA6:ITGB1) is present in oocytes and is involved in sperm-egg fusion (By similarity). Integrin alpha-6/beta-4 (ITGA6:ITGB4) is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome (By similarity). ITGA6:ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed:20682778). ITGA6:ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed:22351760). ITGA6:ITGB4 binds to IGF2 and this binding is essential for IGF2 signaling (PubMed:28873464).

Alternative names

CD49f, Integrin alpha-6, CD49 antigen-like family member F, VLA-6, ITGA6

Recommended products

Rabbit Recombinant Monoclonal Integrin alpha 6 antibody. Suitable for WB and reacts with Human samples. Cited in 5 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR5578
Purification technique
Affinity purification Protein A
Dissociation constant
2.29 x 10-10 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Stable for 12 months at -20°C

Notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Integrin alpha 6 also known as ITGA6 or CD49f functions as a transmembrane receptor protein involved in cell adhesion and signal transduction. As part of the broader integrin family integrin alpha 6 typically pairs with the beta 1 or beta 4 subunits forming the alpha6beta1 or alpha6beta4 integrins respectively. It has a molecular mass of approximately 150 kDa. This protein is mainly expressed in epithelial cells but also found in platelets certain immune cells and in the nervous system. Its expression patterns highlight its role in a variety of cellular and tissue functions.

Biological function summary

The integrin alpha 6 protein partners with the beta 4 subunit to form a heterodimer complex that plays an important role in cellular adhesion to the extracellular matrix specifically interacting with laminins. Its main action helps cells attach to the basement membrane which is an important component of epithelial tissue structure and integrity. The alpha 6 integrin provides structural cell support and participates in transmitting signals that influence cell behavior such as movement survival and proliferation.

Pathways

Integrins including the alpha 6 variant are important components in the integrin signaling pathway and are involved in cell motility and survival pathways. In particular the alpha 6 integrin partners with proteins like focal adhesion kinase (FAK) and phosphoinositide 3-kinase (PI3K) to initiate downstream signaling cascades. The interaction with laminins activates pathways critical for developmental processes and tissue remodeling highlighting their role in maintaining cellular environments.

Associated diseases and disorders

Integrin alpha 6 has significant implications in cancer progression and skin blistering disorders. In various cancers such as breast and skin cancers upregulation of integrin alpha 6 promotes tumor growth and metastasis through interaction with proteins like collagen and laminins found in the tumor microenvironment. Furthermore in the context of blistering disorders such as epidermolysis bullosa mutations in the beta 4 subunit that pairs with alpha 6 can disrupt the integrity of epithelial tissues leading to severe impact on the skin’s structural stability.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-Integrin alpha 6 antibody [EPR5578] (ab124924), expandable thumbnail

    Western blot - Anti-Integrin alpha 6 antibody [EPR5578] (ab124924)

    Lanes 1 - 3: Merged signal (red and green). Green - ab124924 observed at 130 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

    ab124924 was shown to react with Integrin alpha 6 in wild-type SW480 cells in western blot with loss of signal observed in ITGA6 knockout cell line Human ITGA6 (Integrin alpha 6) knockout HeLa cell line ab265838 (knockout cell lysate Human ITGA6 (Integrin alpha 6) knockout HeLa cell lysate ab256957). Wild-type and ITGA6 knockout SW480 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab124924 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Integrin alpha 6 antibody [EPR5578] (ab124924) at 1/1000 dilution

    Lane 1: Wild-type SW480 cell lysate at 20 µg

    Lane 2: ITGA6 knockout SW480 cell lysate at 20 µg

    Lane 2: Western blot - Human ITGA6 (Integrin alpha 6) knockout HeLa cell line (Human ITGA6 (Integrin alpha 6) knockout HeLa cell line ab265838)

    Lane 3: HEK-293 cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 126 kDa, 127 kDa

    Observed band size: 130 kDa

  • Western blot - Anti-Integrin alpha 6 antibody [EPR5578] (ab124924), expandable thumbnail

    Western blot - Anti-Integrin alpha 6 antibody [EPR5578] (ab124924)

    Lanes 1 - 3: Merged signal (red and green). Green - ab124924 observed at 130 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

    ab124924 was shown to react with Integrin alpha 6 in wild-type SW480 cells in western blot with loss of signal observed in ITGA6 knockout cell line Human ITGA6 (Integrin alpha 6) knockout HeLa cell line ab265838 (knockout cell lysate Human ITGA6 (Integrin alpha 6) knockout HeLa cell lysate ab256957). Wild-type and ITGA6 knockout SW480 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab124924 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Integrin alpha 6 antibody [EPR5578] (ab124924) at 1/1000 dilution

    Lane 1: Wild-type SW480 cell lysate at 20 µg

    Lane 2: ITGA6 knockout SW480 cell lysate at 20 µg

    Lane 3: HEK-293 cell lysate at 20 µg

    Secondary

    All lanes: Goat anti-Rabbit IgG H&L preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution

    Performed under reducing conditions.

    Predicted band size: 126 kDa

    Observed band size: 130 kDa

  • Western blot - Anti-Integrin alpha 6 antibody [EPR5578] (ab124924), expandable thumbnail

    Western blot - Anti-Integrin alpha 6 antibody [EPR5578] (ab124924)

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-Integrin alpha 6 antibody [EPR5578] (ab124924) at 1/10000 dilution

    Lane 1: HT-1376 cell lysate at 20 µg

    Lane 2: JAR cell lysate at 20 µg

    Secondary

    All lanes: HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

    Predicted band size: 126 kDa

    Observed band size: 126 kDa

  • Western blot - Anti-Integrin alpha 6 antibody [EPR5578] (ab124924), expandable thumbnail

    Western blot - Anti-Integrin alpha 6 antibody [EPR5578] (ab124924)

    Secondary antibody - anti-rabbit HRP (Goat Anti-Rabbit IgG H&L (HRP) ab6721)

    All lanes: Western blot - Anti-Integrin alpha 6 antibody [EPR5578] (ab124924) at 1/1000 dilution

    Lane 1: JAR cell lysate at 10 µg

    Lane 2: HT1376 cell lysate at 10 µg

    Lane 3: Human platelet lysate at 10 µg

    Secondary

    All lanes: HRP-conjugated goat anti-rabbit at 1/2000 dilution

    Predicted band size: 126 kDa, 93 kDa, 98 kDa

    Observed band size: 118 kDa

  • OI-RD Scanning - Anti-Integrin alpha 6 antibody [EPR5578] (ab124924), expandable thumbnail

    OI-RD Scanning - Anti-Integrin alpha 6 antibody [EPR5578] (ab124924)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

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Product protocols

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