Rat Monoclonal Integrin alpha 6 antibody. Suitable for Flow Cyt and reacts with Human samples. Cited in 16 publications. Immunogen corresponding to Cell preparation containing Itga6 protein.
Images
![Flow Cytometry - Anti-Integrin alpha 6 antibody [GoH3] (AB105669), expandable thumbnail](https://content.abcam.com/products/images/integrin-alpha-6-antibody-goh3-ab105669--flow-cytometry-img1005.gif "Flow Cytometry - Anti-Integrin alpha 6 antibody [GoH3] (AB105669)")
Publications
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- Nature materials 22:1409-14202023The laminin-keratin link shields the nucleus from mechanical deformation and signalling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZanetta Kechagia,Pablo Sáez,Manuel Gómez-González,Brenda Canales,Srivatsava Viswanadha,Martín Zamarbide,Ion Andreu,Thijs Koorman,Amy E M Beedle,Alberto Elosegui-Artola,Patrick W B Derksen,Xavier Trepat,Marino Arroyo,Pere Roca-CusachsPubMed 37709930
- British journal of cancer 128:1742-17522023Comparative analysis of EpCAM high-expressing and low-expressing circulating tumour cells with regard to their clonal relationship and clinical value.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAndré Franken,Annika Kraemer,Alicia Sicking,Meike Watolla,Mahdi Rivandi,Liwen Yang,Jens Warfsmann,Bernhard M Polzer,Thomas W P Friedl,Franziska Meier-Stiegen,Nikolas H Stoecklein,Davut Dayan,Sabine Riethdorf,Volkmar Mueller,Klaus Pantel,André Koch,Andreas D Hartkopf,Natalia Krawczyk,Eugen Ruckhaeberle,Dieter Niederacher,Tanja Fehm,Hans NeubauerPubMed 36823365
- The Journal of biological chemistry 298:1019502022Identification of distinct N-glycosylation patterns on extracellular vesicles from small-cell and non-small-cell lung cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKiyotaka Kondo,Yoichiro Harada,Miyako Nakano,Takehiro Suzuki,Tomoko Fukushige,Ken Hanzawa,Hirokazu Yagi,Koichi Takagi,Keiko Mizuno,Yasuhide Miyamoto,Naoyuki Taniguchi,Koichi Kato,Takuro Kanekura,Naoshi Dohmae,Kentaro Machida,Ikuro Maruyama,Hiromasa InouePubMed 35447118
- Development (Cambridge, England) 147:2020Ontogenesis of the tear drainage system requires Prickle1-driven polarized basement membrane deposition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDianlei Guo et. al.PubMed 33144400
- Stem cell research & therapy 10:3822019Relative safety of various spermatogenic stem cell purification methods for application in spermatogenic stem cell transplantation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJia Tian et. al.PubMed 31842987
- Acta neuropathologica 138:1033-10522019The dystroglycan receptor maintains glioma stem cells in the vascular niche.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBryan W Day et. al.PubMed 31463571
- Methods in molecular biology (Clifton, N.J.) 1926:77-8420193D Cell Culture Models of Epithelial Tissues.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKai Zhang,Aki ManninenPubMed 30742264
- Journal of virology 91:2017Human Adenovirus Type 37 Uses αβ and αβ Integrins for Infection of Human Corneal Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRickard J Storm et. al.PubMed 27974569
- Cell reports 10:239-522015Notch2 is required for inflammatory cytokine-driven goblet cell metaplasia in the lung.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHenry Danahay,Angelica D Pessotti,Julie Coote,Brooke E Montgomery,Donghui Xia,Aaron Wilson,Haidi Yang,Zhao Wang,Luke Bevan,Chris Thomas,Stephanie Petit,Anne London,Peter LeMotte,Arno Doelemeyer,Germán L Vélez-Reyes,Paula Bernasconi,Christy J Fryer,Matt Edwards,Paola Capodieci,Amy Chen,Marc Hild,Aron B JaffePubMed 25558064
- Stem cells and development 23:1134-482014Equine epidermis: a source of epithelial-like stem/progenitor cells with in vitro and in vivo regenerative capacities.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSarah Y Broeckx,Sofie Maes,Tiziana Martinello,Désirée Aerts,Koen Chiers,Tom Mariën,Marco Patruno,Alfredo Franco-Obregón,Jan H SpaasPubMed 24368059
Key facts
- Isotype
- IgG2a
- Host species
- Rat
- Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Cell preparation containing Itga6 protein. The exact immunogen used to generate this antibody is proprietary information. Database link Q61739
Reactivity data
Select an application| Flow Cyt | |
|---|---|
| Human | Tested |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. |
Associated Products
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9 products for Alternative Product
Target data
Function
Integrin alpha-6/beta-1 (ITGA6:ITGB1) is a receptor for laminin on platelets (By similarity). Integrin alpha-6/beta-1 (ITGA6:ITGB1) is present in oocytes and is involved in sperm-egg fusion (By similarity). Integrin alpha-6/beta-4 (ITGA6:ITGB4) is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome (By similarity). ITGA6:ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed:20682778). ITGA6:ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed:22351760). ITGA6:ITGB4 binds to IGF2 and this binding is essential for IGF2 signaling (PubMed:28873464).
Alternative names
CD49f, Integrin alpha-6, CD49 antigen-like family member F, VLA-6, ITGA6
Recommended products
Rat Monoclonal Integrin alpha 6 antibody. Suitable for Flow Cyt and reacts with Human samples. Cited in 16 publications. Immunogen corresponding to Cell preparation containing Itga6 protein.
Key facts
- Isotype
- IgG2a
- Host species
- Rat
- Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Cell preparation containing Itga6 protein. The exact immunogen used to generate this antibody is proprietary information. Database link Q61739
- Clone number
- GoH3
- Purification technique
- Affinity purification Immunogen
- Light chain type
- kappa
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Integrin alpha 6 also known as ITGA6 or CD49f functions as a transmembrane receptor protein involved in cell adhesion and signal transduction. As part of the broader integrin family integrin alpha 6 typically pairs with the beta 1 or beta 4 subunits forming the alpha6beta1 or alpha6beta4 integrins respectively. It has a molecular mass of approximately 150 kDa. This protein is mainly expressed in epithelial cells but also found in platelets certain immune cells and in the nervous system. Its expression patterns highlight its role in a variety of cellular and tissue functions.
- Biological function summary
The integrin alpha 6 protein partners with the beta 4 subunit to form a heterodimer complex that plays an important role in cellular adhesion to the extracellular matrix specifically interacting with laminins. Its main action helps cells attach to the basement membrane which is an important component of epithelial tissue structure and integrity. The alpha 6 integrin provides structural cell support and participates in transmitting signals that influence cell behavior such as movement survival and proliferation.
- Pathways
Integrins including the alpha 6 variant are important components in the integrin signaling pathway and are involved in cell motility and survival pathways. In particular the alpha 6 integrin partners with proteins like focal adhesion kinase (FAK) and phosphoinositide 3-kinase (PI3K) to initiate downstream signaling cascades. The interaction with laminins activates pathways critical for developmental processes and tissue remodeling highlighting their role in maintaining cellular environments.
- Associated diseases and disorders
Integrin alpha 6 has significant implications in cancer progression and skin blistering disorders. In various cancers such as breast and skin cancers upregulation of integrin alpha 6 promotes tumor growth and metastasis through interaction with proteins like collagen and laminins found in the tumor microenvironment. Furthermore in the context of blistering disorders such as epidermolysis bullosa mutations in the beta 4 subunit that pairs with alpha 6 can disrupt the integrity of epithelial tissues leading to severe impact on the skin’s structural stability.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Flow Cytometry - Anti-Integrin alpha 6 antibody [GoH3] (ab105669)
Staining of normal human peripheral blood cells with 0.25 ug of Rat IgG2a Isotype Control Purified (open histogram)or 0.25 ug of Anti-Human/Mouse CD49f (Integrin alpha 6) Purified (filled histogram) followed by Anti-Rat IgG PE Cells in the lymphocyte gate were used for analysis
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