AB105669

Anti-Integrin alpha 6 antibody [GoH3]

Name: Anti-Integrin alpha 6 antibody [GoH3] (ab105669) | Abcam
Brand: Abcam Limited
SKU: AB105669
Rating: 4 (6 reviews)

(6 Reviews)

(19 Publications)

Rat Monoclonal Integrin alpha 6 antibody. Suitable for Flow Cyt and reacts with Human samples. Cited in 19 publications. Immunogen corresponding to Cell preparation containing Itga6 protein.

View Alternative Names

CD49f, Integrin alpha-6, CD49 antigen-like family member F, VLA-6, ITGA6

1 Images

Flow Cytometry - Anti-Integrin alpha 6 antibody [GoH3] (AB105669)

Flow Cyt

Unknown

Flow Cytometry - Anti-Integrin alpha 6 antibody [GoH3] (AB105669)

Staining of normal human peripheral blood cells with 0.25 ug of Rat IgG2a Isotype Control Purified (open histogram)or 0.25 ug of Anti-Human/Mouse CD49f (Integrin alpha 6) Purified (filled histogram) followed by Anti-Rat IgG PE Cells in the lymphocyte gate were used for analysis

578 PE

PE Anti-Integrin alpha 6 antibody [GoH3]

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

GoH3

Isotype

IgG2a

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing Itga6 protein. The exact immunogen used to generate this antibody is proprietary information.

Q61739

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.<br><a href='/en-us/products/primary-antibodies/rat-igg2a-kappa-monoclonal-rtk2758-isotype-control-ab18450'>ab18450</a> - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.09% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Integrin alpha 6 also known as ITGA6 or CD49f functions as a transmembrane receptor protein involved in cell adhesion and signal transduction. As part of the broader integrin family integrin alpha 6 typically pairs with the beta 1 or beta 4 subunits forming the alpha6beta1 or alpha6beta4 integrins respectively. It has a molecular mass of approximately 150 kDa. This protein is mainly expressed in epithelial cells but also found in platelets certain immune cells and in the nervous system. Its expression patterns highlight its role in a variety of cellular and tissue functions.

Biological function summary

The integrin alpha 6 protein partners with the beta 4 subunit to form a heterodimer complex that plays an important role in cellular adhesion to the extracellular matrix specifically interacting with laminins. Its main action helps cells attach to the basement membrane which is an important component of epithelial tissue structure and integrity. The alpha 6 integrin provides structural cell support and participates in transmitting signals that influence cell behavior such as movement survival and proliferation.

Pathways

Integrins including the alpha 6 variant are important components in the integrin signaling pathway and are involved in cell motility and survival pathways. In particular the alpha 6 integrin partners with proteins like focal adhesion kinase (FAK) and phosphoinositide 3-kinase (PI3K) to initiate downstream signaling cascades. The interaction with laminins activates pathways critical for developmental processes and tissue remodeling highlighting their role in maintaining cellular environments.

Integrin alpha 6 has significant implications in cancer progression and skin blistering disorders. In various cancers such as breast and skin cancers upregulation of integrin alpha 6 promotes tumor growth and metastasis through interaction with proteins like collagen and laminins found in the tumor microenvironment. Furthermore in the context of blistering disorders such as epidermolysis bullosa mutations in the beta 4 subunit that pairs with alpha 6 can disrupt the integrity of epithelial tissues leading to severe impact on the skin’s structural stability.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Integrin alpha-6/beta-1 (ITGA6 : ITGB1) is a receptor for laminin on platelets (By similarity). Integrin alpha-6/beta-1 (ITGA6 : ITGB1) is present in oocytes and is involved in sperm-egg fusion (By similarity). Integrin alpha-6/beta-4 (ITGA6 : ITGB4) is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome (By similarity). ITGA6 : ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed : 20682778). ITGA6 : ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed : 22351760). ITGA6 : ITGB4 binds to IGF2 and this binding is essential for IGF2 signaling (PubMed : 28873464).

See full target information ITGA6

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Publications (19)

Recent publications for all applications. Explore the full list and refine your search

Nature materials 22:1409-1420 PubMed37709930

2023

The laminin-keratin link shields the nucleus from mechanical deformation and signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Zanetta Kechagia,Pablo Sáez,Manuel Gómez-González,Brenda Canales,Srivatsava Viswanadha,Martín Zamarbide,Ion Andreu,Thijs Koorman,Amy E M Beedle,Alberto Elosegui-Artola,Patrick W B Derksen,Xavier Trepat,Marino Arroyo,Pere Roca-Cusachs

British journal of cancer 128:1742-1752 PubMed36823365

2023

Comparative analysis of EpCAM high-expressing and low-expressing circulating tumour cells with regard to their clonal relationship and clinical value.

Applications

Unspecified application

Species

Unspecified reactive species

André Franken,Annika Kraemer,Alicia Sicking,Meike Watolla,Mahdi Rivandi,Liwen Yang,Jens Warfsmann,Bernhard M Polzer,Thomas W P Friedl,Franziska Meier-Stiegen,Nikolas H Stoecklein,Davut Dayan,Sabine Riethdorf,Volkmar Mueller,Klaus Pantel,André Koch,Andreas D Hartkopf,Natalia Krawczyk,Eugen Ruckhaeberle,Dieter Niederacher,Tanja Fehm,Hans Neubauer

Small methods 6:e2200152 PubMed35451210

2022

Protein Ligand Nanopattern Size Selects for Cellular Adhesion via Hemidesmosomes over Focal Adhesions.

Applications

Unspecified application

Species

Unspecified reactive species

Sadegh Ghorbani,Ali Shahrokhtash,Julien E Gautrot,Duncan S Sutherland

The Journal of biological chemistry 298:101950 PubMed35447118

2022

Identification of distinct N-glycosylation patterns on extracellular vesicles from small-cell and non-small-cell lung cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kiyotaka Kondo,Yoichiro Harada,Miyako Nakano,Takehiro Suzuki,Tomoko Fukushige,Ken Hanzawa,Hirokazu Yagi,Koichi Takagi,Keiko Mizuno,Yasuhide Miyamoto,Naoyuki Taniguchi,Koichi Kato,Takuro Kanekura,Naoshi Dohmae,Kentaro Machida,Ikuro Maruyama,Hiromasa Inoue

Biomaterials 275:120969 PubMed34157563

2021

Insensitivity of dental pulp stem cells migration to substrate stiffness.

Applications

Unspecified application

Species

Unspecified reactive species

Claire Ehlinger,Eric Mathieu,Morgane Rabineau,Vincent Ball,Philippe Lavalle,Youssef Haikel,Dominique Vautier,Leyla Kocgozlu

Development (Cambridge, England) 147: PubMed33144400

2020

Ontogenesis of the tear drainage system requires Prickle1-driven polarized basement membrane deposition.

Applications

Unspecified application

Species

Unspecified reactive species

Dianlei Guo,Jiali Ru,Fuxiang Mao,Hong Ouyang,Rong Ju,Kaili Wu,Yizhi Liu,Chunqiao Liu

Stem cell research & therapy 10:382 PubMed31842987

2019

Relative safety of various spermatogenic stem cell purification methods for application in spermatogenic stem cell transplantation.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Tian,Ke Ma,Cheng-Bin Pei,Shao-Hua Zhang,Xue Li,Yue Zhou,Bei Yan,Hong-Yan Wang,Liang-Hong Ma

Acta neuropathologica 138:1033-1052 PubMed31463571

2019

The dystroglycan receptor maintains glioma stem cells in the vascular niche.

Applications

Unspecified application

Species

Unspecified reactive species

Bryan W Day,Justin D Lathia,Zara C Bruce,Rochelle C J D'Souza,Ulrich Baumgartner,Kathleen S Ensbey,Yi Chieh Lim,Brett W Stringer,Seçkin Akgül,Carolin Offenhäuser,Yuchen Li,Paul R Jamieson,Fiona M Smith,Courtney L R Jurd,Thomas Robertson,Po-Ling Inglis,Zarnie Lwin,Rosalind L Jeffree,Terrance G Johns,Krishna P L Bhat,Jeremy N Rich,Kevin P Campbell,Andrew W Boyd

Methods in molecular biology (Clifton, N.J.) 1926:77-84 PubMed30742264

2019

3D Cell Culture Models of Epithelial Tissues.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Zhang,Aki Manninen

Journal of virology 91: PubMed27974569

2017

Human Adenovirus Type 37 Uses αβ and αβ Integrins for Infection of Human Corneal Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Rickard J Storm,B David Persson,Lars Nygård Skalman,Lars Frängsmyr,Mona Lindström,Greg Rankin,Richard Lundmark,Fatima Pedrosa Domellöf,Niklas Arnberg

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Integrin alpha 6 antibody [GoH3]

Flow Cytometry - Anti-Integrin alpha 6 antibody [GoH3] (AB105669)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (19)

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