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Mouse Monoclonal Integrin alpha 6 antibody. Suitable for Flow Cyt, ICC/IF and reacts with Human samples. Cited in 23 publications. Immunogen corresponding to Cell preparation containing Integrin alpha 6 protein.


Images

Flow Cytometry - Anti-Integrin alpha 6 antibody [MP 4F10] (AB20142), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha 6 antibody [MP 4F10] (AB20142), expandable thumbnail

Publications

Key facts

Isotype
IgG2b
Host species
Mouse
Storage buffer

Preservative: 0.02% Sodium azide
Constituents: 99.98% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Cell preparation containing Integrin alpha 6 protein. The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytICC/IF
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1 µg for 106 Cells
Notes

(paraformaldehyde or methanol fixed cells)

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Tested
Tested

Species
Human
Dilution info
1 µg/mL
Notes

-

Associated Products

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Target data

Function

Integrin alpha-6/beta-1 (ITGA6:ITGB1) is a receptor for laminin on platelets (By similarity). Integrin alpha-6/beta-1 (ITGA6:ITGB1) is present in oocytes and is involved in sperm-egg fusion (By similarity). Integrin alpha-6/beta-4 (ITGA6:ITGB4) is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome (By similarity). ITGA6:ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed:20682778). ITGA6:ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed:22351760). ITGA6:ITGB4 binds to IGF2 and this binding is essential for IGF2 signaling (PubMed:28873464).

Alternative names

CD49f, Integrin alpha-6, CD49 antigen-like family member F, VLA-6, ITGA6

Recommended products

Mouse Monoclonal Integrin alpha 6 antibody. Suitable for Flow Cyt, ICC/IF and reacts with Human samples. Cited in 23 publications. Immunogen corresponding to Cell preparation containing Integrin alpha 6 protein.

Key facts

Isotype
IgG2b
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Cell preparation containing Integrin alpha 6 protein. The exact immunogen used to generate this antibody is proprietary information.
Clone number
MP 4F10
Purification technique
Affinity purification Protein A
Light chain type
unknown
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Integrin alpha 6 also known as ITGA6 or CD49f functions as a transmembrane receptor protein involved in cell adhesion and signal transduction. As part of the broader integrin family integrin alpha 6 typically pairs with the beta 1 or beta 4 subunits forming the alpha6beta1 or alpha6beta4 integrins respectively. It has a molecular mass of approximately 150 kDa. This protein is mainly expressed in epithelial cells but also found in platelets certain immune cells and in the nervous system. Its expression patterns highlight its role in a variety of cellular and tissue functions.

Biological function summary

The integrin alpha 6 protein partners with the beta 4 subunit to form a heterodimer complex that plays an important role in cellular adhesion to the extracellular matrix specifically interacting with laminins. Its main action helps cells attach to the basement membrane which is an important component of epithelial tissue structure and integrity. The alpha 6 integrin provides structural cell support and participates in transmitting signals that influence cell behavior such as movement survival and proliferation.

Pathways

Integrins including the alpha 6 variant are important components in the integrin signaling pathway and are involved in cell motility and survival pathways. In particular the alpha 6 integrin partners with proteins like focal adhesion kinase (FAK) and phosphoinositide 3-kinase (PI3K) to initiate downstream signaling cascades. The interaction with laminins activates pathways critical for developmental processes and tissue remodeling highlighting their role in maintaining cellular environments.

Associated diseases and disorders

Integrin alpha 6 has significant implications in cancer progression and skin blistering disorders. In various cancers such as breast and skin cancers upregulation of integrin alpha 6 promotes tumor growth and metastasis through interaction with proteins like collagen and laminins found in the tumor microenvironment. Furthermore in the context of blistering disorders such as epidermolysis bullosa mutations in the beta 4 subunit that pairs with alpha 6 can disrupt the integrity of epithelial tissues leading to severe impact on the skin’s structural stability.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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2 product images

  • Flow Cytometry - Anti-Integrin alpha 6 antibody [MP 4F10] (ab20142), expandable thumbnail

    Flow Cytometry - Anti-Integrin alpha 6 antibody [MP 4F10] (ab20142)

    Overlay histogram showing HT1080 cells stained with ab20142 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab20142, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was a Goat Anti-Mouse DyLight® 488 (IgG; H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (Mouse IgG2b [PLPV219] - Isotype Control ab91366, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HT1080 cells fixed with methanol (5 min) used under the same conditions.

    Please note that Abcam does not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.

  • Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha 6 antibody [MP 4F10] (ab20142), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha 6 antibody [MP 4F10] (ab20142)

    ICC/IF image of ab20142 stained HeLa cells (HeLa whole cell lysate ab150035). The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M (Normal Goat Serum ab7481) glycine (Glycine, inhibitory neurotransmitter ab120050) in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab20142, 1μg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Mouse IgG (H+L) Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

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Product protocols

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