AB20142

Anti-Integrin alpha 6 antibody [MP 4F10]

Name: Anti-Integrin alpha 6 antibody [MP 4F10] (ab20142) | Abcam
Brand: Abcam Limited
SKU: AB20142
Rating: 4 (8 reviews)

(8 Reviews)

(30 Publications)

Mouse Monoclonal Integrin alpha 6 antibody. Suitable for Flow Cyt, ICC/IF and reacts with Human samples. Cited in 30 publications. Immunogen corresponding to Cell preparation containing Integrin alpha 6 protein.

View Alternative Names

CD49f, Integrin alpha-6, CD49 antigen-like family member F, VLA-6, ITGA6

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha 6 antibody [MP 4F10] (AB20142)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha 6 antibody [MP 4F10] (AB20142)

ICC/IF image of ab20142 stained HeLa cells (ab150035). The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M (ab7481) glycine (ab120050) in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab20142, 1μg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor^® 488 Goat anti-Mouse IgG (H+L) ab150113) used at a 1/1000 dilution for 1h. Alexa Fluor^® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

Flow Cytometry - Anti-Integrin alpha 6 antibody [MP 4F10] (AB20142)

Flow Cyt

Unknown

Flow Cytometry - Anti-Integrin alpha 6 antibody [MP 4F10] (AB20142)

Overlay histogram showing HT1080 cells stained with ab20142 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab20142, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was a Goat Anti-Mouse DyLight^® 488 (IgG; H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HT1080 cells fixed with methanol (5 min) used under the same conditions.

Please note that Abcam does not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MP 4F10

Isotype

IgG2b

Light chain type

unknown

Carrier free

Reacts with

Human

Applications

Flow Cyt, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "(paraformaldehyde or methanol fixed cells)<a href='/en-us/products/primary-antibodies/mouse-igg2b-kappa-monoclonal-7e10g10-isotype-control-ab170192'>ab170192</a> - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Integrin alpha 6 also known as ITGA6 or CD49f functions as a transmembrane receptor protein involved in cell adhesion and signal transduction. As part of the broader integrin family integrin alpha 6 typically pairs with the beta 1 or beta 4 subunits forming the alpha6beta1 or alpha6beta4 integrins respectively. It has a molecular mass of approximately 150 kDa. This protein is mainly expressed in epithelial cells but also found in platelets certain immune cells and in the nervous system. Its expression patterns highlight its role in a variety of cellular and tissue functions.

Biological function summary

The integrin alpha 6 protein partners with the beta 4 subunit to form a heterodimer complex that plays an important role in cellular adhesion to the extracellular matrix specifically interacting with laminins. Its main action helps cells attach to the basement membrane which is an important component of epithelial tissue structure and integrity. The alpha 6 integrin provides structural cell support and participates in transmitting signals that influence cell behavior such as movement survival and proliferation.

Pathways

Integrins including the alpha 6 variant are important components in the integrin signaling pathway and are involved in cell motility and survival pathways. In particular the alpha 6 integrin partners with proteins like focal adhesion kinase (FAK) and phosphoinositide 3-kinase (PI3K) to initiate downstream signaling cascades. The interaction with laminins activates pathways critical for developmental processes and tissue remodeling highlighting their role in maintaining cellular environments.

Integrin alpha 6 has significant implications in cancer progression and skin blistering disorders. In various cancers such as breast and skin cancers upregulation of integrin alpha 6 promotes tumor growth and metastasis through interaction with proteins like collagen and laminins found in the tumor microenvironment. Furthermore in the context of blistering disorders such as epidermolysis bullosa mutations in the beta 4 subunit that pairs with alpha 6 can disrupt the integrity of epithelial tissues leading to severe impact on the skin’s structural stability.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Integrin alpha-6/beta-1 (ITGA6 : ITGB1) is a receptor for laminin on platelets (By similarity). Integrin alpha-6/beta-1 (ITGA6 : ITGB1) is present in oocytes and is involved in sperm-egg fusion (By similarity). Integrin alpha-6/beta-4 (ITGA6 : ITGB4) is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome (By similarity). ITGA6 : ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed : 20682778). ITGA6 : ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed : 22351760). ITGA6 : ITGB4 binds to IGF2 and this binding is essential for IGF2 signaling (PubMed : 28873464).

See full target information ITGA6

style

left-column

Publications (30)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:10729 PubMed39737945

2024

ITGB4/CD104 mediates zika virus attachment and infection.

Applications

Unspecified application

Species

Unspecified reactive species

Haolong Cong,Jiuqiang Wang,Ning Du,Lei Song,Ruigang Wang,Yang Yang,Rong Lei,Tie-Shan Tang,Chang-Mei Liu,Shuifang Zhu,Xiaodong Han

BMC biology 22:262 PubMed39548509

2024

Rotating culture regulates the formation of HepaRG-derived liver organoids via YAP translocation.

Applications

Unspecified application

Species

Unspecified reactive species

Shaoyu Zhong,Lu Zheng,Yi Wu,Shujin Sun,Qing Luo,Guanbin Song,Dongyuan Lü,Mian Long

Analytical cellular pathology (Amsterdam) 2024:6260651 PubMed39376501

2024

Malignant Transformation of Normal Oral Tissue to Dysplasia and Early Oral Squamous Cell Carcinoma: An Transcriptomics Approach.

Applications

Unspecified application

Species

Unspecified reactive species

Shokoofeh Jamshidi,Matina Tavangar,Setareh Shojaei,Amir Taherkhani

Journal of virology 97:e0077023 PubMed37823645

2023

Efficient clathrin-mediated entry of enteric adenoviruses in human duodenal cells.

Applications

Unspecified application

Species

Unspecified reactive species

Miriam Becker,Dario Valter Conca,Noemi Dorma,Nitesh Mistry,Elin Hahlin,Lars Frängsmyr,Marta Bally,Niklas Arnberg,Gisa Gerold

Cell death & disease 13:486 PubMed35597804

2022

Integrin α3/α6 and αV are implicated in ADAM15-activated FAK and EGFR signalling pathway individually and promote non-small-cell lung cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Jieqi Zhou,Anqi Wang,Tingting Cai,Yue Li,Wenwen Du,Yang Zhang,Ruochen Zhang,Weijie Zhang,Jianjie Zhu,Yuanyuan Zeng,Jian-An Huang,Zeyi Liu

Journal of experimental & clinical cancer research : CR 40:192 PubMed34108040

2021

CD151 drives cancer progression depending on integrin α3β1 through EGFR signaling in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jianjie Zhu,Tingting Cai,Jieqi Zhou,Wenwen Du,Yuanyuan Zeng,Ting Liu,Yulong Fu,Yue Li,Qian Qian,Xiuwei H Yang,Qinglin Li,Jian-An Huang,Zeyi Liu

Cell death & disease 11:972 PubMed33184263

2020

Exosome-derived ENO1 regulates integrin α6β4 expression and promotes hepatocellular carcinoma growth and metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Keqiu Jiang,Chengyong Dong,Zeli Yin,Rui Li,Jiakai Mao,Chengye Wang,Junlin Zhang,Zhenming Gao,Rui Liang,Qi Wang,Liming Wang

BMC research notes 13:528 PubMed33176868

2020

Analysis of ADAM9 regulation and function in vestibular schwannoma primary cells.

Applications

Unspecified application

Species

Unspecified reactive species

Anja Nattmann,Maria Breun,Camelia M Monoranu,Cordula Matthies,Ralf-Ingo Ernestus,Mario Löhr,Carsten Hagemann

Stem cell research & therapy 11:415 PubMed32967725

2020

Both Wnt signaling and epidermal stem cell-derived extracellular vesicles are involved in epidermal cell growth.

Applications

Unspecified application

Species

Unspecified reactive species

Ling Leng,Jie Ma,Luye Lv,Wenjuan Wang,Dunqin Gao,Yunping Zhu,Zhihong Wu

World journal of otorhinolaryngology - head and ne 6:34-40 PubMed32426701

2020

Screening of molecular markers of induced chemotherapy in supraglottic laryngeal squamouscell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Guang Yang,Jugao Fang,Qiang Shi,Ru Wang,Meng Lian,Hongzhi Ma,Ling Feng,Xixi Shen,Yu Wang

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Integrin alpha 6 antibody [MP 4F10]

Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha 6 antibody [MP 4F10] (AB20142)

Flow Cytometry - Anti-Integrin alpha 6 antibody [MP 4F10] (AB20142)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (30)

Product promise