AB27947

Anti-Integrin alpha 9+beta 1 antibody [Y9A2]

Name: Anti-Integrin alpha 9+beta 1 antibody [Y9A2] (ab27947) | Abcam
Brand: Abcam Limited
SKU: AB27947
Price: 445 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(16 Publications)

Mouse Monoclonal Integrin alpha 9 antibody. Suitable for IP, Flow Cyt, ICC/IF and reacts with Rat, Guinea pig, Human samples. Cited in 16 publications. Immunogen corresponding to Cell preparation containing ITGA9 protein.

View Alternative Names

Integrin alpha-9, Integrin alpha-RLC, ITGA9, CD29, FNRB, MDF2, MSK12, ITGB1, Integrin beta-1, Fibronectin receptor subunit beta, Glycoprotein IIa, VLA-4 subunit beta, GPIIA

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

Y9A2

Isotype

IgG1

Carrier free

Reacts with

Guinea pig, Human, Rat

Applications

Flow Cyt, ICC/IF, IP

applications

Immunogen

Cell preparation containing ITGA9 protein. The exact immunogen used to generate this antibody is proprietary information.

Q13797

Specificity

Ab27947 recognises alpha9 integrin expressed as a heterodimer in association with the beta1 integrin.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCFoFr" : {"fullname" : "Immunohistochemistry (PFA perfusion fixed frozen sections)", "shortname":"IHC-FoFr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCR" : {"fullname" : "Immunohistochemistry (Resin sections)", "shortname":"IHC-R"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "(in 100ul). <a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "IHCFoFr-species-checked": "notRecommended", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCR-species-checked": "notRecommended", "IHCR-species-dilution-info": "", "IHCR-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "(in 100ul). <a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "IHCFoFr-species-checked": "notRecommended", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCR-species-checked": "notRecommended", "IHCR-species-dilution-info": "", "IHCR-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Guinea pig": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "(in 100ul). <a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "IHCFoFr-species-checked": "notRecommended", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCR-species-checked": "notRecommended", "IHCR-species-dilution-info": "", "IHCR-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

This clone (Y9A2) inhibits adhesion of alpha9 expressing cells to N terminal osteopontin.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Ab27947 was purified by affinity chromatography, from tissue culture supernatant.

Storage buffer

pH: 7.4 Preservative: 0.09% Sodium azide

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Integrin alpha 9+beta 1 also known as Integrin α9β1 is a heterodimeric protein that functions as a cell adhesion receptor. It has a molecular mass of approximately 170 kDa. This integrin expressed in various tissues including smooth muscle epithelial tissues and leukocytes facilitates cell adhesion to the extracellular matrix by binding to specific ligands. Integrin α9β1 mediates interactions with several extracellular matrix proteins such as tenascin-C and osteopontin.

Biological function summary

Integrin α9β1 influences cell migration proliferation and survival. It plays significant roles in tissue morphogenesis and repair. As part of the integrin family it interacts with the cytoskeleton and transduces signals that regulate cellular responses to the environment. This integrin forms part of larger protein complexes on the cell surface influencing cellular dynamics through its interaction with intracellular proteins.

Pathways

Integrin α9β1 participates in key signaling cascades such as the MAPK/ERK pathway and the PI3K/Akt pathway. These pathways are essential in cell survival proliferation and migration. Interaction with proteins like FAK (focal adhesion kinase) and paxillin amplifies its signal transduction capabilities connecting external matrix signals to intracellular processes thereby integrating with multiple cellular functions.

Integrin α9β1 takes part in the pathology of several conditions. It is implicated in the progression of cancer and chronic inflammatory diseases. Overexpression and dysregulation of Integrin α9β1 often correlate with increased tumor metastasis and inflammation. The integrin associates with matrix metalloproteinases which contribute to the tissue remodeling seen in these disorders highlighting its impact on disease development and progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Integrin alpha-9/beta-1 (ITGA9 : ITGB1) is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. ITGA9 : ITGB1 may play a crucial role in SVEP1/polydom-mediated myoblast cell adhesion (By similarity). Integrin ITGA9 : ITGB1 represses PRKCA-mediated L-type voltage-gated channel Ca(2+) influx and ROCK-mediated calcium sensitivity in vascular smooth muscle cells via its interaction with SVEP1, thereby inhibiting vasocontraction (PubMed : 35802072).

See full target information ITGA9

Additional targets

ITGB1

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Publications (16)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:20913 PubMed40595882

2025

Glypican 3 as target therapy to prevent cell migration and proliferation in rhabdomyosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Maira Bacchiega,Stefania D'Agostino,Antonella Grigoletto,Elena Poli,Paolo Bonvini,Gianni Bisogno,Gianfranco Pasut,Michela Pozzobon

Cell and tissue research 395:63-79 PubMed38040999

2023

Huogu injection protects against SONFH by promoting osteogenic differentiation of BMSCs and preventing osteoblast apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Zhang,Ziyu Li,Xilin Xu,Zhao Liu,Yuanyuan Hao,Fubiao Yang,Xiaodong Li,Ning Zhang,Yunlong Hou,Xiaofeng Zhang

Frontiers in cell and developmental biology 9:681790 PubMed34458254

2021

Osteopontin N-Terminal Function in an Abdominal Aortic Aneurysm From Apolipoprotein E-Deficient Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Hongyang Liu,Ying Zhang,Wei Song,Yancui Sun,Yinong Jiang

Cell death discovery 6:140 PubMed33293521

2020

FN-EDA mediates angiogenesis of hepatic fibrosis via integrin-VEGFR2 in a CD63 synergetic manner.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaonan Su,Xiaowen Ma,Xiaoyu Xie,Hao Wu,Le Wang,Yuemin Feng,Zhen Yu,Chenxi Liu,Jianni Qi,Qiang Zhu

Human cell 32:548-556 PubMed31489579

2019

MiR-148a inhibits the proliferation and migration of glioblastoma by targeting ITGA9.

Applications

Unspecified application

Species

Unspecified reactive species

Tong-Jiang Xu,Peng Qiu,Yu-Bao Zhang,Sheng-Yuan Yu,Guang-Ming Xu,Wei Yang

Journal of cellular and molecular medicine 23:5956-5969 PubMed31293077

2019

Differentiation of human adipose derived stem cells into Leydig-like cells with molecular compounds.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Chen,Chao Li,Weiping Ji,Long Wang,Xianwu Chen,Shenzhi Zhao,Zhangye Xu,Renshan Ge,Xiaoling Guo

Experimental eye research 173:1-12 PubMed29678720

2018

A completely human-derived biomaterial mimicking limbal niche: Platelet-rich fibrin gel.

Applications

Unspecified application

Species

Unspecified reactive species

Gamze Dereli Can,Özge Ekin Akdere,Mehmet Erol Can,Bahri Aydın,Nurullah Cagil,Menemşe Gümüşderelioğlu

Cell adhesion & migration 12:93-100 PubMed27715389

2018

Cell adhesion to anosmin via α5β1, α4β1, and α9β1 integrins.

Applications

Unspecified application

Species

Unspecified reactive species

Yukinori Endo,Hiroko Ishiwata-Endo,Kenneth M Yamada

Nature communications 8:14105 PubMed28102238

2017

Fibronectin-guided migration of carcinoma collectives.

Applications

Unspecified application

Species

Unspecified reactive species

Sandeep Gopal,Laurence Veracini,Dominique Grall,Catherine Butori,Sébastien Schaub,Stéphane Audebert,Luc Camoin,Emilie Baudelet,Agata Radwanska,Stéphanie Beghelli-de la Forest Divonne,Shelia M Violette,Paul H Weinreb,Samah Rekima,Marius Ilie,Anne Sudaka,Paul Hofman,Ellen Van Obberghen-Schilling

The Journal of neuroscience : the official journal of the Society for Neuroscience 35:8359-75 PubMed26019348

2015

Exclusion of integrins from CNS axons is regulated by Arf6 activation and the AIS.

Applications

Unspecified application

Species

Unspecified reactive species

Elske H P Franssen,Rong-Rong Zhao,Hiroaki Koseki,Venkateswarlu Kanamarlapudi,Casper C Hoogenraad,Richard Eva,James W Fawcett

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Integrin alpha 9+beta 1 antibody [Y9A2]

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (16)

Product promise