AB302640

Anti-Integrin alpha V antibody [21/CD51]

Recombinant
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(3 Publications)

Mouse Recombinant Monoclonal Integrin alpha V antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 3 publications.

View Alternative Names

CD51, MSK8, VNRA, VTNR, ITGAV, Integrin alpha-V, Vitronectin receptor, Vitronectin receptor subunit alpha

2 Images

Supplier Data

Western blot - Anti-Integrin alpha V antibody [21/CD51] (AB302640)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Exposure time : 3 minutes

All lanes:

Western blot - Anti-Integrin alpha V antibody [21/CD51] (ab302640) at 1/1000 dilution

Lane 1:

A549 (human lung carcinoma epithelial cell), whole cell lysate at 10 µg

Lane 2:

A431 (human epidermoid carcinoma epithelial cell), whole cell lysate at 10 µg

Lane 3:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 10 µg

Lane 4:

C6 (rat glial tumor cell), whole cell lysate at 10 µg

Lane 5:

C2C12 (mouse myoblasts), whole cell lysate at 10 µg

Lane 6:

NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 10 µg

Lane 7:

Rat cerebellum tissue lysate at 10 µg

Lane 8:

Rat kidney tissue lysate at 10 µg

Lane 9:

Mouse cerebellum tissue lysate at 10 µg

Lane 10:

Human kidney tissue lysate at 10 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 135 kDa

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Supplier Data

Western blot - Anti-Integrin alpha V antibody [21/CD51] (AB302640)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Integrin alpha-V is a glycoprotein of approximately 135 kDa and detected as a 116kDa band after treated with Protein Deglycosylation MIX II.

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

All lanes:

Western blot - Anti-Integrin alpha V antibody [21/CD51] (ab302640) at 1/1000 dilution

Lane 1:

Untreated HeLa whole cell lysate at 20 µg

Lane 2:

HeLa whole cell lysate treated with Protein Deglycosylation Mix II at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 135 kDa,116 kDa

true

Exposure time: 3min

Carrier free

Anti-Integrin alpha V antibody [21/CD51] - BSA and Azide free

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

21 CD5

Isotype

IgG1

Carrier free

Reacts with

Human, Mouse, Rat

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Integrin alpha V also known as integrin av CD51 and CD51/CD61 is a transmembrane protein with an approximate mass of 125 kDa. It functions mechanically by mediating cell adhesion and signaling through its interaction with the extracellular matrix and other cells. Integrin alpha V is widely expressed in a variety of tissues including endothelial and epithelial cells as well as some tumor cells. This protein forms heterodimers typically with beta subunits like CD61 which is also referred to as beta 3 integrin to execute its biological functions.

Biological function summary

Integrin alpha V plays a pivotal role in cellular processes such as migration proliferation and survival. It participates in the formation of multi-molecular complexes within cellular structures. The integrin av integrins particularly in conjunction with its CD51/CD61 form contribute to the regulation of angiogenesis immune response and wound healing. These functions arise from the integrin’s ability to act as a receptor for numerous ligands including fibrinogen fibronectin and vitronectin.

Pathways

Integrin alpha V is a critical component of the integrin signaling and focal adhesion pathways. These pathways involve a network of interactions that include proteins like focal adhesion kinase (FAK) and Src family kinases. Integrin alpha V’s interaction within these pathways assists in transmitting mechanical signals from the extracellular environment to the intracellular signaling cascades affecting cytoskeleton dynamics and gene expression. The pathway interactions with integrin av also coordinate complex signaling with other receptors and matrix proteins like laminins enhancing cellular communication and response to environmental changes.

Integrin alpha V’s dysregulation associates with conditions such as cancer and fibrosis. Overexpression or aberrant functioning of integrin av contributes to tumor progression and metastasis by promoting angiogenesis and cell migration. In fibrotic diseases the protein's interactions with the extracellular matrix components are altered leading to excessive tissue scarring and dysfunction. Furthermore integrin alpha V interplays with proteins such as matrix metalloproteinases (MMPs) in the tumor microenvironment facilitating tissue remodeling and cancer cell invasion.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The alpha-V (ITGAV) integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. ITGAV : ITGB3 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling (PubMed : 23125415). ITGAV : ITGB3 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed : 20682778). ITGAV : ITGB3 binds to FGF1 and this binding is essential for FGF1 signaling (PubMed : 18441324). ITGAV : ITGB3 binds to FGF2 and this binding is essential for FGF2 signaling (PubMed : 28302677). ITGAV : ITGB3 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed : 19578119). ITGAV : ITGB3 binds to IGF2 and this binding is essential for IGF2 signaling (PubMed : 28873464). ITGAV : ITGB3 binds to IL1B and this binding is essential for IL1B signaling (PubMed : 29030430). ITGAV : ITGB3 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed : 18635536, PubMed : 25398877). ITGAV : ITGB3 and ITGAV : ITGB6 act as receptors for fibrillin-1 (FBN1) and mediate R-G-D-dependent cell adhesion to FBN1 (PubMed : 12807887, PubMed : 17158881). Integrin alpha-V/beta-6 or alpha-V/beta-8 (ITGAV : ITGB6 or ITGAV : ITGB8) mediates R-G-D-dependent release of transforming growth factor beta-1 (TGF-beta-1) from regulatory Latency-associated peptide (LAP), thereby playing a key role in TGF-beta-1 activation (PubMed : 15184403, PubMed : 22278742, PubMed : 28117447). ITGAV : ITGB3 acts as a receptor for CD40LG (PubMed : 31331973). ITGAV : ITGB3 acts as a receptor for IBSP and promotes cell adhesion and migration to IBSP (PubMed : 10640428).. (Microbial infection) Integrin ITGAV : ITGB5 acts as a receptor for Adenovirus type C.. (Microbial infection) Integrin ITGAV : ITGB5 and ITGAV : ITGB3 act as receptors for Coxsackievirus A9 and B1.. (Microbial infection) Integrin ITGAV : ITGB3 acts as a receptor for Herpes virus 8/HHV-8.. (Microbial infection) Integrin ITGAV : ITGB6 acts as a receptor for herpes simplex 1/HHV-1.. (Microbial infection) Integrin ITGAV : ITGB3 acts as a receptor for Human parechovirus 1.. (Microbial infection) Integrin ITGAV : ITGB3 acts as a receptor for West nile virus.. (Microbial infection) In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.

See full target information ITGAV

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Diabetology & metabolic syndrome 17:279 PubMed40682113

2025

Irisin regulates integrin αvβ5/FAK/ERK to inhibit neutrophil extracellular traps formation and reduce pancreatic beta-cells pyroptosis in type 2 diabetes mellitus.

Applications

Unspecified application

Species

Unspecified reactive species

Anjun Tan,Tianrong Li,Jingjing Yang,Xiaolu Li,Wenqin Li,Jinwen Yu

Acta biomaterialia 181:222-234 PubMed38648912

2024

In vivo micro-computed tomography evaluation of radiopaque, polymeric device degradation in normal and inflammatory environments.

Applications

Unspecified application

Species

Unspecified reactive species

Kendell M Pawelec,Jeremy M L Hix,Arianna Troia,Keith W MacRenaris,Matti Kiupel,Erik M Shapiro

Biomaterials advances 159:213825 PubMed38479242

2024

Material matters: Degradation products affect regenerating Schwann cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kendell M Pawelec,Jeremy M L Hix,Erik M Shapiro

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Integrin alpha V antibody [21/CD51]

Western blot - Anti-Integrin alpha V antibody [21/CD51] (AB302640)

Western blot - Anti-Integrin alpha V antibody [21/CD51] (AB302640)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise