Mouse Monoclonal Integrin alpha V antibody. Carrier free. Suitable for Flow Cyt (Intra) and reacts with Human samples. Cited in 29 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human ITGAV.
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![Flow Cytometry (Intracellular) - Anti-Integrin alpha V antibody [272-17E6] - BSA and Azide free (AB16821), expandable thumbnail](https://content.abcam.com/products/images/integrin-alpha-v-antibody-272-17e6-bsa-and-azide-free-ab16821--flow-cytometry-intracellular-img76697.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Integrin alpha V antibody [272-17E6] - BSA and Azide free (AB16821)")
![Flow Cytometry (Intracellular) - Anti-Integrin alpha V antibody [272-17E6] - BSA and Azide free (AB16821), expandable thumbnail](https://content.abcam.com/products/images/integrin-alpha-v-antibody-272-17e6-bsa-and-azide-free-ab16821--flow-cytometry-intracellular-img82350.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Integrin alpha V antibody [272-17E6] - BSA and Azide free (AB16821)")
Publications
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- Cell reports 41:1115112022A molecular interactome of the glioblastoma perivascular niche reveals integrin binding sialoprotein as a mediator of tumor cell migration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYasmin Ghochani,Sree Deepthi Muthukrishnan,Alireza Sohrabi,Riki Kawaguchi,Michael C Condro,Soniya Bastola,Fuying Gao,Yue Qin,Jack Mottahedeh,M Luisa Iruela-Arispe,Nagesh Rao,Dan R Laks,Linda M Liau,Gary W Mathern,Steven A Goldman,S Thomas Carmichael,Ichiro Nakano,Giovanni Coppola,Stephanie K Seidlits,Harley I KornblumPubMed 36261010
- Nanomaterials (Basel, Switzerland) 11:2021Homing Peptide-Based Targeting of Tenascin-C and Fibronectin in Endometriosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLorena Simón-Gracia,Kristina Kiisholts,Vilma Petrikaitė,Allan Tobi,Merli Saare,Prakash Lingasamy,Maire Peters,Andres Salumets,Tambet TeesaluPubMed 34947606
- Journal of materials chemistry. B 9:7205-72152021Hyaluronic acid drives mesenchymal stromal cell-derived extracellular matrix assembly by promoting fibronectin fibrillogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarisa Assunção,Chi Him Kendrick Yiu,Ho-Ying Wan,Dan Wang,Dai Fei Elmer Ker,Rocky S Tuan,Anna BlockiPubMed 33710248
- Scientific reports 11:113282021Platelet derived growth factor receptor β (PDGFRβ) is a host receptor for the human malaria parasite adhesin TRAP.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRyan W J Steel,Vladimir Vigdorovich,Nicholas Dambrauskas,Brandon K Wilder,Silvia A Arredondo,Debashree Goswami,Sudhir Kumar,Sara Carbonetti,Kristian E Swearingen,Thao Nguyen,Will Betz,Nelly Camargo,Bridget S Fisher,Jo Soden,Helen Thomas,Jim Freeth,Robert L Moritz,D Noah Sather,Stefan H I KappePubMed 34059712
- Molecular oncology 15:1543-15652021EWI-2 controls nucleocytoplasmic shuttling of EGFR signaling molecules and miRNA sorting in exosomes to inhibit prostate cancer cell metastasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChenying Fu,Qing Zhang,Ani Wang,Songpeng Yang,Yangfu Jiang,Lin Bai,Quan WeiPubMed 33605506
- Cancers 13:2021Integrin αvβ3 Engagement Regulates Glucose Metabolism and Migration through Focal Adhesion Kinase (FAK) and Protein Arginine Methyltransferase 5 (PRMT5) in Glioblastoma Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPulin Che,Lei Yu,Gregory K Friedman,Meimei Wang,Xiaoxue Ke,Huafeng Wang,Wenbin Zhang,Burt Nabors,Qiang Ding,Xiaosi HanPubMed 33807786
- Nature communications 12:11402021Entry of spores into intestinal epithelial cells contributes to recurrence of Clostridioides difficile infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPablo Castro-Córdova,Paola Mora-Uribe,Rodrigo Reyes-Ramírez,Glenda Cofré-Araneda,Josué Orozco-Aguilar,Christian Brito-Silva,María José Mendoza-León,Sarah A Kuehne,Nigel P Minton,Marjorie Pizarro-Guajardo,Daniel Paredes-SabjaPubMed 33602902
- Theranostics 10:231-2462020Soluble CD146, a cerebrospinal fluid marker for neuroinflammation, promotes blood-brain barrier dysfunction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDaji Wang et. al.PubMed 31903117
- Molecular pharmaceutics 16:292-3042018Cluster of Differentiation 46 Is the Major Receptor in Human Blood-Brain Barrier Endothelial Cells for Uptake of Exosomes Derived from Brain-Metastatic Melanoma Cells (SK-Mel-28).Applications:Unspecified applicationReactive species:Unspecified reactive speciesHiroki Kuroda et. al.PubMed 30452273
- Journal of cell science 131:2018α5β1 integrin trafficking and Rac activation are regulated by APPL1 in a Rab5-dependent manner to inhibit cell migration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNicole L Diggins et. al.PubMed 29361527
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Constituents: PBS
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Native Full Length Protein corresponding to Human ITGAV. Database link P06756
Reactivity data
Select an application| Flow Cyt (Intra) | |
|---|---|
| Human | Tested |
| Mouse | Not recommended |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Associated Products
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Target data
Function
The alpha-V (ITGAV) integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. ITGAV:ITGB3 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling (PubMed:23125415). ITGAV:ITGB3 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed:20682778). ITGAV:ITGB3 binds to FGF1 and this binding is essential for FGF1 signaling (PubMed:18441324). ITGAV:ITGB3 binds to FGF2 and this binding is essential for FGF2 signaling (PubMed:28302677). ITGAV:ITGB3 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed:19578119). ITGAV:ITGB3 binds to IGF2 and this binding is essential for IGF2 signaling (PubMed:28873464). ITGAV:ITGB3 binds to IL1B and this binding is essential for IL1B signaling (PubMed:29030430). ITGAV:ITGB3 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed:18635536, PubMed:25398877). ITGAV:ITGB3 and ITGAV:ITGB6 act as receptors for fibrillin-1 (FBN1) and mediate R-G-D-dependent cell adhesion to FBN1 (PubMed:12807887, PubMed:17158881). Integrin alpha-V/beta-6 or alpha-V/beta-8 (ITGAV:ITGB6 or ITGAV:ITGB8) mediates R-G-D-dependent release of transforming growth factor beta-1 (TGF-beta-1) from regulatory Latency-associated peptide (LAP), thereby playing a key role in TGF-beta-1 activation (PubMed:15184403, PubMed:22278742, PubMed:28117447). ITGAV:ITGB3 acts as a receptor for CD40LG (PubMed:31331973). ITGAV:ITGB3 acts as a receptor for IBSP and promotes cell adhesion and migration to IBSP (PubMed:10640428). (Microbial infection) Integrin ITGAV:ITGB5 acts as a receptor for Adenovirus type C. (Microbial infection) Integrin ITGAV:ITGB5 and ITGAV:ITGB3 act as receptors for Coxsackievirus A9 and B1. (Microbial infection) Integrin ITGAV:ITGB3 acts as a receptor for Herpes virus 8/HHV-8. (Microbial infection) Integrin ITGAV:ITGB6 acts as a receptor for herpes simplex 1/HHV-1. (Microbial infection) Integrin ITGAV:ITGB3 acts as a receptor for Human parechovirus 1. (Microbial infection) Integrin ITGAV:ITGB3 acts as a receptor for West nile virus. (Microbial infection) In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
Alternative names
CD51, MSK8, VNRA, VTNR, ITGAV, Integrin alpha-V, Vitronectin receptor, Vitronectin receptor subunit alpha
Recommended products
Mouse Monoclonal Integrin alpha V antibody. Carrier free. Suitable for Flow Cyt (Intra) and reacts with Human samples. Cited in 29 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human ITGAV.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Constituents: PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Native Full Length Protein corresponding to Human ITGAV. Database link P06756
- Carrier free
- Yes
- Clone number
- 272-17E6
- Purification technique
- Affinity purification Protein G
- Specificity
Cross reacts with all alpha V integrins.
- Light chain type
- kappa
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was changed from ascites to tissue culture supernatant on 17 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Integrin alpha V also known as integrin av CD51 and CD51/CD61 is a transmembrane protein with an approximate mass of 125 kDa. It functions mechanically by mediating cell adhesion and signaling through its interaction with the extracellular matrix and other cells. Integrin alpha V is widely expressed in a variety of tissues including endothelial and epithelial cells as well as some tumor cells. This protein forms heterodimers typically with beta subunits like CD61 which is also referred to as beta 3 integrin to execute its biological functions.
- Biological function summary
Integrin alpha V plays a pivotal role in cellular processes such as migration proliferation and survival. It participates in the formation of multi-molecular complexes within cellular structures. The integrin av integrins particularly in conjunction with its CD51/CD61 form contribute to the regulation of angiogenesis immune response and wound healing. These functions arise from the integrin’s ability to act as a receptor for numerous ligands including fibrinogen fibronectin and vitronectin.
- Pathways
Integrin alpha V is a critical component of the integrin signaling and focal adhesion pathways. These pathways involve a network of interactions that include proteins like focal adhesion kinase (FAK) and Src family kinases. Integrin alpha V’s interaction within these pathways assists in transmitting mechanical signals from the extracellular environment to the intracellular signaling cascades affecting cytoskeleton dynamics and gene expression. The pathway interactions with integrin av also coordinate complex signaling with other receptors and matrix proteins like laminins enhancing cellular communication and response to environmental changes.
- Associated diseases and disorders
Integrin alpha V’s dysregulation associates with conditions such as cancer and fibrosis. Overexpression or aberrant functioning of integrin av contributes to tumor progression and metastasis by promoting angiogenesis and cell migration. In fibrotic diseases the protein's interactions with the extracellular matrix components are altered leading to excessive tissue scarring and dysfunction. Furthermore integrin alpha V interplays with proteins such as matrix metalloproteinases (MMPs) in the tumor microenvironment facilitating tissue remodeling and cancer cell invasion.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
![Flow Cytometry (Intracellular) - Anti-Integrin alpha V antibody [272-17E6] - BSA and Azide free (ab16821), expandable thumbnail](https://content.abcam.com/products/images/integrin-alpha-v-antibody-272-17e6-bsa-and-azide-free-ab16821--flow-cytometry-intracellular-img76697.jpg "Flow Cytometry (Intracellular) - Anti-Integrin alpha V antibody [272-17E6] - BSA and Azide free (ab16821)")
Flow Cytometry (Intracellular) - Anti-Integrin alpha V antibody [272-17E6] - BSA and Azide free (ab16821)
Intracellular Flow Cytometry analysis of HeLa cells labeling Integrin alpha V with ab16821 at 0.5 μg (red), or with 1 μg of Mouse IgG isotype control.
![Flow Cytometry (Intracellular) - Anti-Integrin alpha V antibody [272-17E6] - BSA and Azide free (ab16821), expandable thumbnail](https://content.abcam.com/products/images/integrin-alpha-v-antibody-272-17e6-bsa-and-azide-free-ab16821--flow-cytometry-intracellular-img82350.jpg "Flow Cytometry (Intracellular) - Anti-Integrin alpha V antibody [272-17E6] - BSA and Azide free (ab16821)")
This image is a courtesy of Anonymous customer reviewFlow Cytometry (Intracellular) - Anti-Integrin alpha V antibody [272-17E6] - BSA and Azide free (ab16821)
ab16821 staining Integrin alpha V in Human melanoma cells by intacellular Flow Cytometery. Samples were prepared by trypsinizing cells, treatment with STI and spun out trypsin, kept on ice during experiment duration. FSC and SSC were used to gate healthy cell population, which excluded PI positive cells. The primary antibody was diluted 1/250 and incubated with sample for 1 hour at 4°C in 2% FCS/0. 1%NaAzide/PBS. An Alexa Fuor® 488-conjugated Goat polyclonal to mouse IgG was used at dilution at 1/500 as secondary antibody.
This image was generated using the ascites version of the product.
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