AB16821

Anti-Integrin alpha V antibody [272-17E6] - BSA and Azide free

Name: Anti-Integrin alpha V antibody [272-17E6] - BSA and Azide free (ab16821) | Abcam
Brand: Abcam Limited
SKU: AB16821
Price: 905 USD
Availability: InStock
Rating: 5 (4 reviews)

(4 Reviews)

(32 Publications)

Mouse Monoclonal Integrin alpha V antibody. Carrier free. Suitable for Flow Cyt (Intra) and reacts with Human samples. Cited in 32 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human ITGAV.

View Alternative Names

CD51, MSK8, VNRA, VTNR, ITGAV, Integrin alpha-V, Vitronectin receptor, Vitronectin receptor subunit alpha

2 Images

Flow Cytometry (Intracellular) - Anti-Integrin alpha V antibody [272-17E6] - BSA and Azide free (AB16821)

Flow Cyt (Intra)

AbReview16056****

Flow Cytometry (Intracellular) - Anti-Integrin alpha V antibody [272-17E6] - BSA and Azide free (AB16821)

ab16821 staining Integrin alpha V in Human melanoma cells by intacellular Flow Cytometery. Samples were prepared by trypsinizing cells, treatment with STI and spun out trypsin, kept on ice during experiment duration. FSC and SSC were used to gate healthy cell population, which excluded PI positive cells. The primary antibody was diluted 1/250 and incubated with sample for 1 hour at 4°C in 2% FCS/0. 1%NaAzide/PBS. An Alexa Fuor® 488-conjugated Goat polyclonal to mouse IgG was used at dilution at 1/500 as secondary antibody.

This image was generated using the ascites version of the product.

This image is a courtesy of Anonymous Abreview

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Integrin alpha V antibody [272-17E6] - BSA and Azide free (AB16821)

Intracellular Flow Cytometry analysis of HeLa cells labeling Integrin alpha V with ab16821 at 0.5 μg (red), or with 1 μg of Mouse IgG isotype control.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

272-17E6

Isotype

IgG1

Light chain type

kappa

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt (Intra)

applications

Immunogen

Native Full Length Protein corresponding to Human ITGAV.

P06756

Specificity

Cross reacts with all alpha V integrins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }

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Product details

This product was changed from ascites to tissue culture supernatant on 17 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Integrin alpha V also known as integrin av CD51 and CD51/CD61 is a transmembrane protein with an approximate mass of 125 kDa. It functions mechanically by mediating cell adhesion and signaling through its interaction with the extracellular matrix and other cells. Integrin alpha V is widely expressed in a variety of tissues including endothelial and epithelial cells as well as some tumor cells. This protein forms heterodimers typically with beta subunits like CD61 which is also referred to as beta 3 integrin to execute its biological functions.

Biological function summary

Integrin alpha V plays a pivotal role in cellular processes such as migration proliferation and survival. It participates in the formation of multi-molecular complexes within cellular structures. The integrin av integrins particularly in conjunction with its CD51/CD61 form contribute to the regulation of angiogenesis immune response and wound healing. These functions arise from the integrin’s ability to act as a receptor for numerous ligands including fibrinogen fibronectin and vitronectin.

Pathways

Integrin alpha V is a critical component of the integrin signaling and focal adhesion pathways. These pathways involve a network of interactions that include proteins like focal adhesion kinase (FAK) and Src family kinases. Integrin alpha V’s interaction within these pathways assists in transmitting mechanical signals from the extracellular environment to the intracellular signaling cascades affecting cytoskeleton dynamics and gene expression. The pathway interactions with integrin av also coordinate complex signaling with other receptors and matrix proteins like laminins enhancing cellular communication and response to environmental changes.

Integrin alpha V’s dysregulation associates with conditions such as cancer and fibrosis. Overexpression or aberrant functioning of integrin av contributes to tumor progression and metastasis by promoting angiogenesis and cell migration. In fibrotic diseases the protein's interactions with the extracellular matrix components are altered leading to excessive tissue scarring and dysfunction. Furthermore integrin alpha V interplays with proteins such as matrix metalloproteinases (MMPs) in the tumor microenvironment facilitating tissue remodeling and cancer cell invasion.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The alpha-V (ITGAV) integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. ITGAV : ITGB3 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling (PubMed : 23125415). ITGAV : ITGB3 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed : 20682778). ITGAV : ITGB3 binds to FGF1 and this binding is essential for FGF1 signaling (PubMed : 18441324). ITGAV : ITGB3 binds to FGF2 and this binding is essential for FGF2 signaling (PubMed : 28302677). ITGAV : ITGB3 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed : 19578119). ITGAV : ITGB3 binds to IGF2 and this binding is essential for IGF2 signaling (PubMed : 28873464). ITGAV : ITGB3 binds to IL1B and this binding is essential for IL1B signaling (PubMed : 29030430). ITGAV : ITGB3 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed : 18635536, PubMed : 25398877). ITGAV : ITGB3 and ITGAV : ITGB6 act as receptors for fibrillin-1 (FBN1) and mediate R-G-D-dependent cell adhesion to FBN1 (PubMed : 12807887, PubMed : 17158881). Integrin alpha-V/beta-6 or alpha-V/beta-8 (ITGAV : ITGB6 or ITGAV : ITGB8) mediates R-G-D-dependent release of transforming growth factor beta-1 (TGF-beta-1) from regulatory Latency-associated peptide (LAP), thereby playing a key role in TGF-beta-1 activation (PubMed : 15184403, PubMed : 22278742, PubMed : 28117447). ITGAV : ITGB3 acts as a receptor for CD40LG (PubMed : 31331973). ITGAV : ITGB3 acts as a receptor for IBSP and promotes cell adhesion and migration to IBSP (PubMed : 10640428).. (Microbial infection) Integrin ITGAV : ITGB5 acts as a receptor for Adenovirus type C.. (Microbial infection) Integrin ITGAV : ITGB5 and ITGAV : ITGB3 act as receptors for Coxsackievirus A9 and B1.. (Microbial infection) Integrin ITGAV : ITGB3 acts as a receptor for Herpes virus 8/HHV-8.. (Microbial infection) Integrin ITGAV : ITGB6 acts as a receptor for herpes simplex 1/HHV-1.. (Microbial infection) Integrin ITGAV : ITGB3 acts as a receptor for Human parechovirus 1.. (Microbial infection) Integrin ITGAV : ITGB3 acts as a receptor for West nile virus.. (Microbial infection) In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.

See full target information ITGAV

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Publications (32)

Recent publications for all applications. Explore the full list and refine your search

Journal of neuroinflammation 21:196 PubMed39107821

2024

Enhanced phagocytosis associated with multinucleated microglia via Pyk2 inhibition in an acute β-amyloid infusion model.

Applications

Unspecified application

Species

Unspecified reactive species

Ji-Won Lee,Kaito Mizuno,Haruhisa Watanabe,In-Hee Lee,Takuya Tsumita,Kyoko Hida,Yasutaka Yawaka,Yoshimasa Kitagawa,Akira Hasebe,Tadahiro Iimura,Sek Won Kong

Journal of experimental & clinical cancer research : CR 43:211 PubMed39075581

2024

IGF1R signaling induces epithelial-mesenchymal plasticity via ITGAV in cutaneous carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Marta Lopez-Cerda,Laura Lorenzo-Sanz,Victoria da Silva-Diz,Sandra Llop,Rosa M Penin,Josep Oriol Bermejo,Richard de Goeij-de Haas,Sander R Piersma,Thang V Pham,Connie R Jimenez,Juan Martin-Liberal,Purificación Muñoz

Cell reports 41:111511 PubMed36261010

2022

A molecular interactome of the glioblastoma perivascular niche reveals integrin binding sialoprotein as a mediator of tumor cell migration.

Applications

Unspecified application

Species

Unspecified reactive species

Yasmin Ghochani,Sree Deepthi Muthukrishnan,Alireza Sohrabi,Riki Kawaguchi,Michael C Condro,Soniya Bastola,Fuying Gao,Yue Qin,Jack Mottahedeh,M Luisa Iruela-Arispe,Nagesh Rao,Dan R Laks,Linda M Liau,Gary W Mathern,Steven A Goldman,S Thomas Carmichael,Ichiro Nakano,Giovanni Coppola,Stephanie K Seidlits,Harley I Kornblum

Cell reports 40:111153 PubMed35926468

2022

Cancer genes disfavoring T cell immunity identified via integrated systems approach.

Applications

Unspecified application

Species

Unspecified reactive species

Rigel J Kishton,Shashank J Patel,Amy E Decker,Suman K Vodnala,Maggie Cam,Tori N Yamamoto,Yogin Patel,Madhusudhanan Sukumar,Zhiya Yu,Michelle Ji,Amanda N Henning,Devikala Gurusamy,Douglas C Palmer,Roxana A Stefanescu,Andrew T Girvin,Winifred Lo,Anna Pasetto,Parisa Malekzadeh,Drew C Deniger,Kris C Wood,Neville E Sanjana,Nicholas P Restifo

Nanomaterials (Basel, Switzerland) 11: PubMed34947606

2021

Homing Peptide-Based Targeting of Tenascin-C and Fibronectin in Endometriosis.

Applications

Unspecified application

Species

Unspecified reactive species

Lorena Simón-Gracia,Kristina Kiisholts,Vilma Petrikaitė,Allan Tobi,Merli Saare,Prakash Lingasamy,Maire Peters,Andres Salumets,Tambet Teesalu

Journal of materials chemistry. B 9:7205-7215 PubMed33710248

2021

Hyaluronic acid drives mesenchymal stromal cell-derived extracellular matrix assembly by promoting fibronectin fibrillogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Marisa Assunção,Chi Him Kendrick Yiu,Ho-Ying Wan,Dan Wang,Dai Fei Elmer Ker,Rocky S Tuan,Anna Blocki

Scientific reports 11:11328 PubMed34059712

2021

Platelet derived growth factor receptor β (PDGFRβ) is a host receptor for the human malaria parasite adhesin TRAP.

Applications

Unspecified application

Species

Unspecified reactive species

Ryan W J Steel,Vladimir Vigdorovich,Nicholas Dambrauskas,Brandon K Wilder,Silvia A Arredondo,Debashree Goswami,Sudhir Kumar,Sara Carbonetti,Kristian E Swearingen,Thao Nguyen,Will Betz,Nelly Camargo,Bridget S Fisher,Jo Soden,Helen Thomas,Jim Freeth,Robert L Moritz,D Noah Sather,Stefan H I Kappe

Molecular oncology 15:1543-1565 PubMed33605506

2021

EWI-2 controls nucleocytoplasmic shuttling of EGFR signaling molecules and miRNA sorting in exosomes to inhibit prostate cancer cell metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Chenying Fu,Qing Zhang,Ani Wang,Songpeng Yang,Yangfu Jiang,Lin Bai,Quan Wei

Cancers 13: PubMed33807786

2021

Integrin αvβ3 Engagement Regulates Glucose Metabolism and Migration through Focal Adhesion Kinase (FAK) and Protein Arginine Methyltransferase 5 (PRMT5) in Glioblastoma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Pulin Che,Lei Yu,Gregory K Friedman,Meimei Wang,Xiaoxue Ke,Huafeng Wang,Wenbin Zhang,Burt Nabors,Qiang Ding,Xiaosi Han

Nature communications 12:1140 PubMed33602902

2021

Entry of spores into intestinal epithelial cells contributes to recurrence of Clostridioides difficile infection.

Applications

Unspecified application

Species

Unspecified reactive species

Pablo Castro-Córdova,Paola Mora-Uribe,Rodrigo Reyes-Ramírez,Glenda Cofré-Araneda,Josué Orozco-Aguilar,Christian Brito-Silva,María José Mendoza-León,Sarah A Kuehne,Nigel P Minton,Marjorie Pizarro-Guajardo,Daniel Paredes-Sabja

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Integrin alpha V antibody [272-17E6] - BSA and Azide free

Flow Cytometry (Intracellular) - Anti-Integrin alpha V antibody [272-17E6] - BSA and Azide free (AB16821)

Flow Cytometry (Intracellular) - Anti-Integrin alpha V antibody [272-17E6] - BSA and Azide free (AB16821)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (32)

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