Name: Anti-Integrin alpha V antibody [EPR16800]
SKU: ab179475
Rating: 5 (10 reviews)

Anti-Integrin alpha V antibody [EPR16800] is a rabbit recombinant monoclonal antibody that is used to detect Integrin alpha V in ICC/IF, IHC-P, IP, Western blot. Suitable for Human, Mouse, Rat samples.

- Recombinant format for unrivaled batch-batch consistency
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation
- Cited in over 70 publications

Related conjugates and formulations

ab275350
Conjugated
Alexa Fluor® 488 Anti-Integrin alpha V antibody [EPR16800]
ab211845
Conjugated
PE Anti-Integrin alpha V antibody [EPR16800]
ab207286
Conjugated
Alexa Fluor® 594 Anti-Integrin alpha V antibody [EPR16800]
ab205474
Conjugated
HRP Anti-Integrin alpha V antibody [EPR16800]
ab204684
Conjugated
Alexa Fluor® 647 Anti-Integrin alpha V antibody [EPR16800]
ab275351
Conjugated
Alexa Fluor® 555 Anti-Integrin alpha V antibody [EPR16800]
ab271932
Carrier free
Anti-Integrin alpha V antibody [EPR16800] - BSA and Azide free

Images

Western blot - Anti-Integrin alpha V antibody [EPR16800] (AB179475), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Abcam Recommends

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	WB	ICC/IF	IHC-P
Human	Tested	Tested	Tested	Tested
Mouse	Expected	Tested	Expected	Tested
Rat	Expected	Tested	Expected	Expected

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/40	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/5000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/5000	Notes -
Species Human	Dilution info 1/5000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/500	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/500	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/500	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Associated Products

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Target data

See full target information ITGAV

Function

The alpha-V (ITGAV) integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. ITGAV:ITGB3 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling (PubMed:23125415). ITGAV:ITGB3 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed:20682778). ITGAV:ITGB3 binds to FGF1 and this binding is essential for FGF1 signaling (PubMed:18441324). ITGAV:ITGB3 binds to FGF2 and this binding is essential for FGF2 signaling (PubMed:28302677). ITGAV:ITGB3 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed:19578119). ITGAV:ITGB3 binds to IGF2 and this binding is essential for IGF2 signaling (PubMed:28873464). ITGAV:ITGB3 binds to IL1B and this binding is essential for IL1B signaling (PubMed:29030430). ITGAV:ITGB3 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed:18635536, PubMed:25398877). ITGAV:ITGB3 and ITGAV:ITGB6 act as receptors for fibrillin-1 (FBN1) and mediate R-G-D-dependent cell adhesion to FBN1 (PubMed:12807887, PubMed:17158881). Integrin alpha-V/beta-6 or alpha-V/beta-8 (ITGAV:ITGB6 or ITGAV:ITGB8) mediates R-G-D-dependent release of transforming growth factor beta-1 (TGF-beta-1) from regulatory Latency-associated peptide (LAP), thereby playing a key role in TGF-beta-1 activation (PubMed:15184403, PubMed:22278742, PubMed:28117447). ITGAV:ITGB3 acts as a receptor for CD40LG (PubMed:31331973). ITGAV:ITGB3 acts as a receptor for IBSP and promotes cell adhesion and migration to IBSP (PubMed:10640428). (Microbial infection) Integrin ITGAV:ITGB5 acts as a receptor for Adenovirus type C. (Microbial infection) Integrin ITGAV:ITGB5 and ITGAV:ITGB3 act as receptors for Coxsackievirus A9 and B1. (Microbial infection) Integrin ITGAV:ITGB3 acts as a receptor for Herpes virus 8/HHV-8. (Microbial infection) Integrin ITGAV:ITGB6 acts as a receptor for herpes simplex 1/HHV-1. (Microbial infection) Integrin ITGAV:ITGB3 acts as a receptor for Human parechovirus 1. (Microbial infection) Integrin ITGAV:ITGB3 acts as a receptor for West nile virus. (Microbial infection) In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.

Alternative names

CD51, MSK8, VNRA, VTNR, ITGAV, Integrin alpha-V, Vitronectin receptor, Vitronectin receptor subunit alpha

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR16800
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Product Specifications

Anti-Integrin alpha V antibody [EPR16800] (ab179475) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ICC/IF, IHC-P, IP, WB in human, mouse, rat samples.
Anti-Integrin alpha V antibody [EPR16800] (ab179475) specifically detects Integrin alpha V (UniProt ID: P06756; Molecular weight: 113kDa) and is sold in 100 µL and 1 mL selling sizes.

Quality and Validation

Abcam's high quality manufacturing and validation processes ensure Anti-Integrin alpha V antibody [EPR16800] (ab179475) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-Integrin alpha V antibody [EPR16800] (ab179475) has been cited over 73 times in peer reviewed journals and is trusted by the scientific community.
Anti-Integrin alpha V antibody [EPR16800] (ab179475) has 10 independent reviews from customers.

Related Products
Conjugation-ready, carrier free format available for antibody clone EPR16800 - ab245051.
Antibody clone EPR16800 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 647, HRP, Alexa Fluor® 594, PE, Alexa Fluor® 488, Alexa Fluor® 555 (ab204684, ab205474, ab207286, ab211845, ab275350, ab275351).

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Integrin alpha V also known as integrin av CD51 and CD51/CD61 is a transmembrane protein with an approximate mass of 125 kDa. It functions mechanically by mediating cell adhesion and signaling through its interaction with the extracellular matrix and other cells. Integrin alpha V is widely expressed in a variety of tissues including endothelial and epithelial cells as well as some tumor cells. This protein forms heterodimers typically with beta subunits like CD61 which is also referred to as beta 3 integrin to execute its biological functions.

Biological function summary

Integrin alpha V plays a pivotal role in cellular processes such as migration proliferation and survival. It participates in the formation of multi-molecular complexes within cellular structures. The integrin av integrins particularly in conjunction with its CD51/CD61 form contribute to the regulation of angiogenesis immune response and wound healing. These functions arise from the integrin’s ability to act as a receptor for numerous ligands including fibrinogen fibronectin and vitronectin.

Pathways

Integrin alpha V is a critical component of the integrin signaling and focal adhesion pathways. These pathways involve a network of interactions that include proteins like focal adhesion kinase (FAK) and Src family kinases. Integrin alpha V’s interaction within these pathways assists in transmitting mechanical signals from the extracellular environment to the intracellular signaling cascades affecting cytoskeleton dynamics and gene expression. The pathway interactions with integrin av also coordinate complex signaling with other receptors and matrix proteins like laminins enhancing cellular communication and response to environmental changes.

Associated diseases and disorders

Integrin alpha V’s dysregulation associates with conditions such as cancer and fibrosis. Overexpression or aberrant functioning of integrin av contributes to tumor progression and metastasis by promoting angiogenesis and cell migration. In fibrotic diseases the protein's interactions with the extracellular matrix components are altered leading to excessive tissue scarring and dysfunction. Furthermore integrin alpha V interplays with proteins such as matrix metalloproteinases (MMPs) in the tumor microenvironment facilitating tissue remodeling and cancer cell invasion.

Product promise

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23 product images

Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-HMGA1 antibody [RM1061] (Anti-HMGA1 antibody [RM1061] ab315350) at 1/1000 dilution
Lane 1: BxPC-3 (human pancreas adenocarcinoma epithelial cell) nuclear fraction at 20 µg
Lane 2: BxPC-3 (human pancreas adenocarcinoma epithelial cell) cytoplasmic fraction at 20 µg
Lane 3: BxPC-3 (human pancreas adenocarcinoma epithelial cell) membrane fraction at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 17 kDa, 36 kDa, 15 kDa, 125 kDa
Exposure time: 92s
Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475)
Integrin alpha V Western blot staining using rabbit Anti-Integrin alpha V antibody
All lanes: Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475) at 1/1000 dilution
Lane 1: Untreated HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate. at 15 µg
Lane 2: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate treated with PNGase F. at 15 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 116 kDa
Observed band size: 116.135 kDa
Exposure time: 20s
Immunoprecipitation - Anti-Integrin alpha V antibody [EPR16800] (ab179475)
Integrin alpha V was immunoprecipitated from 1 mg of A549 (Human lung carcinoma) whole cell extract with ab179475 at 1/40 dilution. Western blot analysis was performed from the immunoprecipitate using ab179475 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: A549 whole cell extract.
Lane 2: PBS instead of A549 whole cell extract.

Blocking/Dilution buffer: 5% NFDM/TBST.
ab179475 can recognize 135kDa full length Integrin alpha V and 125kDa heavy chain. The 125 kDa band is Integrin alpha V heavy chain.
All lanes: Immunoprecipitation - Anti-Integrin alpha V antibody [EPR16800] (ab179475)
Predicted band size: 116 kDa
Observed band size: 125 kDa
Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475)
Integrin alpha V Western blot staining using rabbit Anti-Integrin alpha V antibody
All lanes: Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475) at 1/1000 dilution
Lane 1: Untreated K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate. at 15 µg
Lane 2: K-562 (Human chronic myelogenous leukemia lymphoblast) treated with PNGase F whole cell lysate. at 15 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 116 kDa
Observed band size: 116.135 kDa
Exposure time: 80s
Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475)
Blocking/dilution buffer: 5% NFDM/TBST.
ab179475 can recognize 135kDa full length Integrin alpha V and 125kDa heavy chain. The 125 kDa band is Integrin alpha V heavy chain.
All lanes: Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475) at 1/50000 dilution
Lane 1: HUVEC (Human umbilical vein endothelial cell line) whole cell lysates at 20 µg
Lane 2: HT-29 (Human colorectal adenocarcinoma cells) whole cell lysates at 20 µg
Lane 3: A549 (Human lung carcinoma) whole cell lysates at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 116 kDa
Observed band size: 125 kDa
This image is courtesy of an anonymous customer review.
Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475)
Diluent: 2% BSA in 1X TBST.
Blocked using 5% BSA for 1 hour at RT.
All lanes: Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475) at 1/2000 dilution
All lanes: Mouse skin whole cell lysate at 40 µg
Secondary
All lanes: Goat anti-Rabbit IgG(H+L)-HRP at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 116 kDa
Exposure time: 25s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha V antibody [EPR16800] (ab179475)
Immunohistochemical analysis of paraffin-embedded human transitional cell carcinoma of bladder tissue labeling Integrin alpha V with ab179475 at 1/500 dilution followed by Goat Anti-Rabbit HRP (IgG H&L) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution.
Membrane and weak cytoplasm staining on human transitional cell carcinoma of bladder is observed. Counterstained with hematoxylin.
Negative control: Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha V antibody [EPR16800] (ab179475)
Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized A549 (Human lung carcinoma) cells labeling Integrin alpha V with ab179475 at 1/500 dilution, followed by Goat anti-rabbit Alexa Fluor^® 488 (IgG) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/400 dilution (green).
Confocal image showing membrane and cytoplasm staining on A549 cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (goat anti-mouse AlexaFluor^®594 secondary antibody) at 1/500 dilution (red).
The negative controls are as follows:
-ve control 1: ab179475 at 1/500 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor^®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor^®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.
Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475)
Blocking/dilution buffer: 5% NFDM/TBST.
ab179475 can recognize 135kDa full length Integrin alpha V and 125kDa heavy chain. The 125 kDa band is Integrin alpha V heavy chain.
All lanes: Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475) at 1/5000 dilution
Lane 1: Human fetal kidney lysates at 10 µg
Lane 2: Human fetal brain lysates at 10 µg
Secondary
All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 116 kDa
Observed band size: 125 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha V antibody [EPR16800] (ab179475)
Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Integrin alpha V with ab179475 at 1/500 dilution followed by Goat Anti-Rabbit HRP (IgG H&L) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution.
Membrane and cytoplasm staining on human kidney tubules is observed. Counterstained with hematoxylin.
Negative control: Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475)
Blocking/dilution buffer: 5% NFDM/TBST.
ab179475 can recognize 135kDa full length Integrin alpha V and 125kDa heavy chain. The 125 kDa band is Integrin alpha V heavy chain.
All lanes: Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475) at 1/5000 dilution
Lane 1: Mouse brain lysates at 10 µg
Lane 2: Mouse kidney lysates at 10 µg
Lane 3: Mouse spleen lysates at 10 µg
Lane 4: Rat brain lysates at 10 µg
Lane 5: Rat kidney lysates at 10 µg
Lane 6: C6 (Rat glial tumor cells) whole cell lysates at 10 µg
Lane 7: NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates at 10 µg
Lane 8: Raji (Human Burkitt's lymphoma cell line) whole cell lysates at 10 µg
Secondary
All lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 116 kDa
Observed band size: 125 kDa, 135 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha V antibody [EPR16800] (ab179475)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Integrin alpha V with ab179475 at 1/500 dilution followed by Goat Anti-Rabbit HRP (IgG H&L) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary at 1/500 dilution.
Membrane and cytoplasm staining on mouse kidney tubule and glomerulus is observed. Counterstained with hematoxylin.
Negative control: Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 and ab179475 were used as loading controls.

The molecular weight observed is consistent with literatures (PMID: 9722601, PMID: 3360311).
Samples are non-boiled as boiling may cause protein aggregation and were run on a Bis-Tris gel.
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution and anti- Integrin alpha V antibody (ab179475) loading control staining at 1/5000 dilution.
All lanes: Western blot - Anti-CaSR antibody [EPR24050-59] (Anti-CaSR antibody [EPR24050-59] ab259846) at 1/1000 dilution
Lane 1: Mouse kidney membrane tissue lysate at 10 µg
Lane 2: Mouse kidney membrane control tissue lysate at 10 µg
Lane 3: Rat kidney membrane tissue lysate at 10 µg
Lane 4: Rat skeletal muscle tissue lysate at 10 µg
Secondary
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Exposure time: 10s
Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475)
Integrin alpha V Western blot staining using rabbit Anti-Integrin alpha V antibody
Low expression: HL-1.
Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.
Alternatively spliced forms of the EVI-1 gene encode at least three distinct proteins: EVI-1 (145 kDa), MDS1/EVI-1 (200 kDa) and EVI-1Δ324 (88 kDa) PMID:(PMID: 24944602).
The identity of the bands between 75 kDa and 180 kDa are unknown.
In Western blot, Anti-Integrin alpha V antibody [EPR16800] (ab179475) staining at 1/5000 dilution.
All lanes: Western blot - Anti-EVI1 antibody [EPR26816-18] (Anti-EVI1 antibody [EPR26816-18] ab315973) at 1/1000 dilution
Lane 1: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 80 µg
Lane 2: HL-1 (mouse atrial muscle cell) whole cell lysate at 80 µg
Secondary
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 200 kDa, 125 kDa
Exposure time: 59s
Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475)
Integrin alpha V Western blot staining using rabbit Anti-Integrin alpha V antibody
The identity of the higher MW band at approximately 50 kDa (in lane 2) is unknown.
Samples are non-boiled as boiling may cause protein aggregation.
In Western blot, Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade ab176842) staining at 1/100000 dilution.
In Western blot, Anti-Integrin alpha V antibody [EPR16800] (ab179475) staining at 1/5000 dilution.
All lanes: Western blot - Anti-GPCR GPRC5D antibody [EPR28376-41] (Anti-GPCR GPRC5D antibody [EPR28376-41] ab315808) at 1/1000 dilution
Lane 1: RPMI-8226 (human plasmacytoma, myeloma B lymphocyte) membrane fraction at 50 µg
Lane 2: RPMI-8226 nuclear fraction at 50 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 33 kDa, 125 kDa, 15 kDa
Exposure time: 15s
Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475)
Integrin alpha V Western blot staining using rabbit Anti-Integrin alpha V antibody
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression of TARC/CCL17 is upregulated in response to M-CSF, GM-CSF and BFA treatment (PMID: 27525438).
In Western blot, Anti-Integrin alpha V antibody [EPR16800] staining at 1/5000 dilution.
All lanes: Western blot - Anti-TARC/CCL17 antibody [EPR28961-9] (Anti-TARC/CCL17 antibody [EPR28961-9] ab323800) at 1/1000 dilution
Lane 1: Untreated Mouse bone marrow lysate at 20 µg
Lane 2: Mouse bone marrow treated with 100ng/mL M-CSF for 7 days, 20ng/ml GM-CSF and 300ng/ml BFA for 16 hours lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 11 kDa, 125 kDa
Exposure time: 180s
Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475)
Integrin alpha V Western blot staining using rabbit Anti-Integrin alpha V antibody
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The identity of the bands between 75 kDa and 25 kDa are unknown.
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) staining at 1/10000 dilution.
In Western blot, Anti-Integrin alpha V antibody [EPR16800] (ab179475) staining at 1/5000 dilution.
All lanes: Western blot - Anti-GluN2D antibody [EPR28378-56] (Anti-GluN2D antibody [EPR28378-56] ab314664) at 1/1000 dilution
Lane 1: Mouse brain tissue lysate at 20 µg
Lane 2: Mouse brain membrane fraction at 20 µg
Lane 3: Mouse brain non-membrane fraction at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 165 kDa
Exposure time: 180s
Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
All lanes: Western blot - Anti-RANK antibody [EPR26196-15] (Anti-RANK antibody [EPR26196-15] ab305233) at 1/1000 dilution
Lane 1: K-562 (human chronic myelogenous leukemia lymphoblast) non-membrane fraction at 20 µg
Lane 2: K-562 membrane fraction at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Developed using the ECL technique.
Observed band size: 80 kDa
Exposure time: 3min
Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The identity of the bands higher than 150 kDa and lower than 50 kDa are unknown.
In Western blot, Anti-Integrin alpha V antibody [EPR16800] (ab179475) staining at 1/5000 dilution.
All lanes: Western blot - Anti-FRS2 antibody [EPR28714-126] (Anti-FRS2 antibody [EPR28714-126] ab320727) at 1/1000 dilution
Lane 1: F9 (mouse embryonal carcinoma epithelial cell) non-membrane fraction at 20 µg
Lane 2: F9 (mouse embryonal carcinoma epithelial cell) membrane fraction at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 74 kDa, 116 kDa, 135 kDa
Exposure time: 180s
Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative controls: kidney (PMID: 24309898), cerebellum (PMID: 24309898).

In Western blot, anti- Integrin alpha V antibody (ab179475) loading control staining at 1/5000 dilution.
Exposure time: 180 seconds
All lanes: Western blot - Anti-Neutrophil Elastase antibody [EPR28386-66] (Anti-Neutrophil Elastase antibody [EPR28386-66] ab310335) at 1/1000 dilution
Lane 1: Rat bone marrow tissue lysate at 20 µg
Lane 2: Rat cerebellum tissue lysate at 20 µg
Lane 3: Rat kidney tissue lysate at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Exposure time: 180s
Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Kir4.1/KCNJ10 is expressed in vivo as a homotetramer (four units of KIR4.1) or a heterotetramer with KIR5.1(two units of KIR4.1 and KIR5.1). (PMID: 33348803; PMID: 15310750 ). This antibody can only detect the tetramer of Kir4.1/KCNJ10.
Samples are non-boiled as boiling may cause protein aggregation.
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution. Anti-Integrin alpha V (ab179475) control staining at 1/1000 dilution
Exposure time: 15 seconds
All lanes: Western blot - Anti-Kir4.1/KCNJ10 antibody [EPR27251-74] (Anti-Kir4.1/KCNJ10 antibody [EPR27251-74] ab312844) at 1/1000 dilution
Lane 1: Mouse brain non-membrane fraction at 20 µg
Lane 2: Mouse brain membrane fraction at 20 µg
Lane 3: Rat brain non-membrane fraction at 20 µg
Lane 4: Rat brain membrane fraction at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 250 kDa
Exposure time: 15s
Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.

In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200, 000 dilution and anti-Integrin alpha V (ab179475) staining at 1/5, 000 dilution.
Samples are non-boiled as boiling may cause protein aggregation.
All lanes: Western blot - Anti-Noradrenaline transporter antibody [EPR23527-534] (Anti-Noradrenaline transporter antibody [EPR23527-534] ab254361) at 1/1000 dilution
Lane 1: Mouse brain membrane tissue lysate at 20 µg
Lane 2: Mouse brain membrane control tissue lysate at 20 µg
Lane 3: Rat brain membrane tissue lysate at 20 µg
Lane 4: Rat brain membrane control tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 69 kDa
Observed band size: 69 kDa
Exposure time: 180s
Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475)
Integrin alpha V Western blot staining using rabbit Anti-Integrin alpha V antibody
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 29285063, 19726871).
Samples are non-boiled as boiling may cause protein aggregation.
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution. Anti-Integrin alpha V (ab179475) control staining at 1/5000 dilution.
All lanes: Western blot - Anti-Cx40/GJA5 antibody [EPR28370-62] (Anti-Cx40/GJA5 antibody [EPR28370-62] ab313644) at 1/1000 dilution
Lane 1: Mouse lung non-membrane fraction at 20 µg
Lane 2: Mouse lung membrane fraction at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Performed under non-reducing conditions.
Observed band size: 40 kDa, 36 kDa
Exposure time: 37s