Anti-Integrin alpha V antibody [EPR16800]

22 product images

• 

  Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475)

  All lanes: Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475) at 1/1000 dilution

  Lane 1: Untreated HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate. at 15 µg

  Lane 2: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate treated with PNGase F. at 15 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

  Predicted band size: 116 kDa

  Observed band size: 116.135 kDa

  Exposure time: 20s

• 

  Immunoprecipitation - Anti-Integrin alpha V antibody [EPR16800] (ab179475)

  Integrin alpha V was immunoprecipitated from 1 mg of A549 (Human lung carcinoma) whole cell extract with ab179475 at 1/40 dilution. Western blot analysis was performed from the immunoprecipitate using ab179475 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
  

  Lane 1: A549 whole cell extract.

  Lane 2: PBS instead of A549 whole cell extract.

  
  Blocking/Dilution buffer: 5% NFDM/TBST.

  ab179475 can recognize 135kDa full length Integrin alpha V and 125kDa heavy chain. The 125 kDa band is Integrin alpha V heavy chain.

  All lanes: Immunoprecipitation - Anti-Integrin alpha V antibody [EPR16800] (ab179475)

  Predicted band size: 116 kDa

  Observed band size: 125 kDa

• 

  Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475)

  All lanes: Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475) at 1/1000 dilution

  Lane 1: Untreated K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate. at 15 µg

  Lane 2: K-562 (Human chronic myelogenous leukemia lymphoblast) treated with PNGase F whole cell lysate. at 15 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

  Predicted band size: 116 kDa

  Observed band size: 116.135 kDa

  Exposure time: 80s

• 

  Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475)

  Blocking/dilution buffer: 5% NFDM/TBST.
  

  ab179475 can recognize 135kDa full length Integrin alpha V and 125kDa heavy chain. The 125 kDa band is Integrin alpha V heavy chain.

  All lanes: Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475) at 1/50000 dilution

  Lane 1: HUVEC (Human umbilical vein endothelial cell line) whole cell lysates at 20 µg

  Lane 2: HT-29 (Human colorectal adenocarcinoma cells) whole cell lysates at 20 µg

  Lane 3: A549 (Human lung carcinoma) whole cell lysates at 20 µg

  Secondary

  All lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

  Predicted band size: 116 kDa

  Observed band size: 125 kDa

• 

  This image is courtesy of an anonymous customer review.

  Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475)

  Diluent: 2% BSA in 1X TBST.
  

  Blocked using 5% BSA for 1 hour at RT.

  All lanes: Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475) at 1/2000 dilution

  All lanes: Mouse skin whole cell lysate at 40 µg

  Secondary

  All lanes: Goat anti-Rabbit IgG(H+L)-HRP at 1/5000 dilution

  Developed using the ECL technique.

  Predicted band size: 116 kDa

  Exposure time: 25s

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha V antibody [EPR16800] (ab179475)

  Immunohistochemical analysis of paraffin-embedded human transitional cell carcinoma of bladder tissue labeling Integrin alpha V with ab179475 at 1/500 dilution followed by Goat Anti-Rabbit HRP (IgG H&L) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution.
  

  Membrane and weak cytoplasm staining on human transitional cell carcinoma of bladder is observed. Counterstained with hematoxylin.

  Negative control: Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

  Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha V antibody [EPR16800] (ab179475)

  Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized A549 (Human lung carcinoma) cells labeling Integrin alpha V with ab179475 at 1/500 dilution, followed by Goat anti-rabbit Alexa Fluor^® 488 (IgG) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/400 dilution (green).
  

  Confocal image showing membrane and cytoplasm staining on A549 cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (goat anti-mouse AlexaFluor^®594 secondary antibody) at 1/500 dilution (red).

  The negative controls are as follows:

  -ve control 1: ab179475 at 1/500 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor^®594 Goat anti-Mouse secondary) at 1/500 dilution.

  -ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor^®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.

• 

  Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475)

  Blocking/dilution buffer: 5% NFDM/TBST.
  

  ab179475 can recognize 135kDa full length Integrin alpha V and 125kDa heavy chain. The 125 kDa band is Integrin alpha V heavy chain.

  All lanes: Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475) at 1/5000 dilution

  Lane 1: Human fetal kidney lysates at 10 µg

  Lane 2: Human fetal brain lysates at 10 µg

  Secondary

  All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

  Predicted band size: 116 kDa

  Observed band size: 125 kDa

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha V antibody [EPR16800] (ab179475)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Integrin alpha V with ab179475 at 1/500 dilution followed by Goat Anti-Rabbit HRP (IgG H&L) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution.
  

  Membrane and cytoplasm staining on human kidney tubules is observed. Counterstained with hematoxylin.

  Negative control: Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

  Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• 

  Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475)

  Blocking/dilution buffer: 5% NFDM/TBST.
  

  ab179475 can recognize 135kDa full length Integrin alpha V and 125kDa heavy chain. The 125 kDa band is Integrin alpha V heavy chain.

  All lanes: Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475) at 1/5000 dilution

  Lane 1: Mouse brain lysates at 10 µg

  Lane 2: Mouse kidney lysates at 10 µg

  Lane 3: Mouse spleen lysates at 10 µg

  Lane 4: Rat brain lysates at 10 µg

  Lane 5: Rat kidney lysates at 10 µg

  Lane 6: C6 (Rat glial tumor cells) whole cell lysates at 10 µg

  Lane 7: NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates at 10 µg

  Lane 8: Raji (Human Burkitt's lymphoma cell line) whole cell lysates at 10 µg

  Secondary

  All lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

  Predicted band size: 116 kDa

  Observed band size: 125 kDa, 135 kDa

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha V antibody [EPR16800] (ab179475)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Integrin alpha V with ab179475 at 1/500 dilution followed by Goat Anti-Rabbit HRP (IgG H&L) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary at 1/500 dilution.
  

  Membrane and cytoplasm staining on mouse kidney tubule and glomerulus is observed. Counterstained with hematoxylin.

  Negative control: Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

  Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• 

  Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.
  
  Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 and ab179475 were used as loading controls.
  
  The molecular weight observed is consistent with literatures (PMID: 9722601, PMID: 3360311).
  Samples are non-boiled as boiling may cause protein aggregation and were run on a Bis-Tris gel.
  In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution and anti- Integrin alpha V antibody (ab179475) loading control staining at 1/5000 dilution.

  All lanes: Western blot - Anti-CaSR antibody [EPR24050-59] (Anti-CaSR antibody [EPR24050-59] ab259846) at 1/1000 dilution

  Lane 1: Mouse kidney membrane tissue lysate at 10 µg

  Lane 2: Mouse kidney membrane control tissue lysate at 10 µg

  Lane 3: Rat kidney membrane tissue lysate at 10 µg

  Lane 4: Rat skeletal muscle tissue lysate at 10 µg

  Secondary

  All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

  Exposure time: 10s

• 

  Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

  All lanes: Western blot - Anti-HMGA1 antibody [RM1061] (Anti-HMGA1 antibody [RM1061] ab315350) at 1/1000 dilution

  Lane 1: BxPC-3 (human pancreas adenocarcinoma epithelial cell) nuclear fraction at 20 µg

  Lane 2: BxPC-3 (human pancreas adenocarcinoma epithelial cell) cytoplasmic fraction at 20 µg

  Lane 3: BxPC-3 (human pancreas adenocarcinoma epithelial cell) membrane fraction at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

  Observed band size: 17 kDa, 36 kDa, 15 kDa, 125 kDa

  Exposure time: 92s

• 

  Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475)

  Low expression: HL-1.

  Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

  Alternatively spliced forms of the EVI-1 gene encode at least three distinct proteins: EVI-1 (145 kDa), MDS1/EVI-1 (200 kDa) and EVI-1Δ324 (88 kDa) PMID:(PMID: 24944602).

  The identity of the bands between 75 kDa and 180 kDa are unknown.

  In Western blot, Anti-Integrin alpha V antibody [EPR16800] (ab179475) staining at 1/5000 dilution.

  All lanes: Western blot - Anti-EVI1 antibody [EPR26816-18] (Anti-EVI1 antibody [EPR26816-18] ab315973) at 1/1000 dilution

  Lane 1: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 80 µg

  Lane 2: HL-1 (mouse atrial muscle cell) whole cell lysate at 80 µg

  Secondary

  All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

  Observed band size: 200 kDa, 125 kDa

  Exposure time: 59s

• 

  Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475)

  The identity of the higher MW band at approximately 50 kDa (in lane 2) is unknown.

  Samples are non-boiled as boiling may cause protein aggregation.

  In Western blot, Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade ab176842) staining at 1/100000 dilution.

  In Western blot, Anti-Integrin alpha V antibody [EPR16800] (ab179475) staining at 1/5000 dilution.

  All lanes: Western blot - Anti-GPCR GPRC5D antibody [EPR28376-41] (Anti-GPCR GPRC5D antibody [EPR28376-41] ab315808) at 1/1000 dilution

  Lane 1: RPMI-8226 (human plasmacytoma, myeloma B lymphocyte) membrane fraction at 50 µg

  Lane 2: RPMI-8226 nuclear fraction at 50 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

  Observed band size: 33 kDa, 125 kDa, 15 kDa

  Exposure time: 15s

• 

  Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST
  The identity of the bands between 75 kDa and 25 kDa are unknown.
  In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.
  In Western blot, Anti-Integrin alpha V antibody [EPR16800] (ab179475) staining at 1/5000 dilution.

  All lanes: Western blot - Anti-GluN2D antibody [EPR28378-56] (Anti-GluN2D antibody [EPR28378-56] ab314664) at 1/1000 dilution

  Lane 1: Mouse brain tissue lysate at 20 µg

  Lane 2: Mouse brain membrane fraction at 20 µg

  Lane 3: Mouse brain non-membrane fraction at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

  Observed band size: 165 kDa

  Exposure time: 180s

• 

  Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST
  This blot was developed using a high sensitivity ECL substrate.
  Exposure time: 3 minutes

  All lanes: Western blot - Anti-RANK antibody [EPR26196-15] (Anti-RANK antibody [EPR26196-15] ab305233) at 1/1000 dilution

  Lane 1: K-562 (human chronic myelogenous leukemia lymphoblast) non-membrane fraction at 20 µg

  Lane 2: K-562 membrane fraction at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

  Developed using the ECL technique.

  Observed band size: 80 kDa

  Exposure time: 3min

• 

  Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST
  The molecular weight observed is consistent with what has been described in the literature (PMID: 29285063, 19726871).
  Samples are non-boiled as boiling may cause protein aggregation.
  
  In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution. Anti-Integrin alpha V (ab179475) control staining at 1/5000 dilution
  Exposure time: 37 seconds

  All lanes: Western blot - Anti-Cx40/GJA5 antibody [EPR28370-62] (Anti-Cx40/GJA5 antibody [EPR28370-62] ab313644) at 1/1000 dilution

  Lane 1: Mouse lung non-membrane fraction at 20 µg

  Lane 2: Mouse lung membrane fraction at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Observed band size: 40, 36 kDa

  Exposure time: 37s

• 

  Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST
  Negative controls: kidney (PMID: 24309898), cerebellum (PMID: 24309898).
  
  In Western blot, anti- Integrin alpha V antibody (ab179475) loading control staining at 1/5000 dilution.
  Exposure time: 180 seconds

  All lanes: Western blot - Anti-Neutrophil Elastase antibody [EPR28386-66] (Anti-Neutrophil Elastase antibody [EPR28386-66] ab310335) at 1/1000 dilution

  Lane 1: Rat bone marrow tissue lysate at 20 µg

  Lane 2: Rat cerebellum tissue lysate at 20 µg

  Lane 3: Rat kidney tissue lysate at 20 µg

  Secondary

  All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

  Exposure time: 180s

• 

  Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST
  Kir4.1/KCNJ10 is expressed in vivo as a homotetramer (four units of KIR4.1) or a heterotetramer with KIR5.1(two units of KIR4.1 and KIR5.1). (PMID: 33348803; PMID: 15310750 ). This antibody can only detect the tetramer of Kir4.1/KCNJ10.
  Samples are non-boiled as boiling may cause protein aggregation.
  In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution. Anti-Integrin alpha V (ab179475) control staining at 1/1000 dilution
  Exposure time: 15 seconds

  All lanes: Western blot - Anti-Kir4.1/KCNJ10 antibody [EPR27251-74] (Anti-Kir4.1/KCNJ10 antibody [EPR27251-74] ab312844) at 1/1000 dilution

  Lane 1: Mouse brain non-membrane fraction at 20 µg

  Lane 2: Mouse brain membrane fraction at 20 µg

  Lane 3: Rat brain non-membrane fraction at 20 µg

  Lane 4: Rat brain membrane fraction at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Observed band size: 250 kDa

  Exposure time: 15s

• 

  Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.
  
  In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200, 000 dilution and anti-Integrin alpha V (ab179475) staining at 1/5, 000 dilution.
  Samples are non-boiled as boiling may cause protein aggregation.

  All lanes: Western blot - Anti-Noradrenaline transporter antibody [EPR23527-534] (Anti-Noradrenaline transporter antibody [EPR23527-534] ab254361) at 1/1000 dilution

  Lane 1: Mouse brain membrane tissue lysate at 20 µg

  Lane 2: Mouse brain membrane control tissue lysate at 20 µg

  Lane 3: Rat brain membrane tissue lysate at 20 µg

  Lane 4: Rat brain membrane control tissue lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Predicted band size: 69 kDa

  Observed band size: 69 kDa

  Exposure time: 180s

• 

  Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  The identity of the bands higher than 150 kDa and lower than 50 kDa are unknown.

  In Western blot, Anti-Integrin alpha V antibody [EPR16800] (ab179475) staining at 1/5000 dilution.

  All lanes: Western blot - Anti-FRS2 antibody [EPR28714-126] (Anti-FRS2 antibody [EPR28714-126] ab320727) at 1/1000 dilution

  Lane 1: F9 (mouse embryonal carcinoma epithelial cell) non-membrane fraction at 20 µg

  Lane 2: F9 (mouse embryonal carcinoma epithelial cell) membrane fraction at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

  Observed band size: 74 kDa, 116 kDa, 135 kDa

  Exposure time: 180s