Anti-Integrin alpha V antibody [EPR16800]

• WB

Supplier Data

Western blot - Anti-Integrin alpha V antibody [EPR16800] (AB179475)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-HMGA1 antibody [RM1061] (<a href='/en-us/products/primary-antibodies/hmga1-antibody-rm1061-ab315350'>ab315350</a>) at 1/1000 dilution

Lane 1:

BxPC-3 (human pancreas adenocarcinoma epithelial cell) nuclear fraction at 20 µg

Lane 2:

BxPC-3 (human pancreas adenocarcinoma epithelial cell) cytoplasmic fraction at 20 µg

Lane 3:

BxPC-3 (human pancreas adenocarcinoma epithelial cell) membrane fraction at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 17 kDa,36 kDa,15 kDa,125 kDa

false

Exposure time: 92s

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha V antibody [EPR16800] (AB179475)

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Integrin alpha V with ab179475 at 1/500 dilution followed by Goat Anti-Rabbit HRP (IgG H&L) (ab97051) secondary antibody at 1/500 dilution.

Membrane and cytoplasm staining on human kidney tubules is observed. Counterstained with hematoxylin.

Negative control : Using PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha V antibody [EPR16800] (AB179475)

Immunohistochemical analysis of formalin fixed paraffin embedded human kideny labelling integrin alpha V with ab179475 at a concentration of 0.1µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with a OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was performed with DISCOVERY cell conditioning solution (CC1) 100°C, pH8.5 for 32mins. ab179475 anti-Integrin alpha V antibody [EPR16800] was incubated for 16mins at 37°C. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha V antibody [EPR16800] (AB179475)

Immunofluorescent analysis of 100% methanol-fixed 0.1% Triton X-100 permeabilized A549 (Human lung carcinoma) cells labeling Integrin alpha V with ab179475 at 1/500 dilution followed by Goat anti-rabbit Alexa Fluor^® 488 (IgG) (ab150077) secondary antibody at 1/400 dilution (green).

Confocal image showing membrane and cytoplasm staining on A549 cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (goat anti-mouse AlexaFluor^®594 secondary antibody) at 1/500 dilution (red).

The negative controls are as follows :

-ve control 1 : ab179475 at 1/500 dilution followed by ab150120 (AlexaFluor^®594 Goat anti-Mouse secondary) at 1/500 dilution.

-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor^®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha V antibody [EPR16800] (AB179475)

Immunohistochemical analysis of paraffin-embedded human transitional cell carcinoma of bladder tissue labeling Integrin alpha V with ab179475 at 1/500 dilution followed by Goat Anti-Rabbit HRP (IgG H&L) (ab97051) secondary antibody at 1/500 dilution.

Membrane and weak cytoplasm staining on human transitional cell carcinoma of bladder is observed. Counterstained with hematoxylin.

Negative control : Using PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IP

Supplier Data

Immunoprecipitation - Anti-Integrin alpha V antibody [EPR16800] (AB179475)

Integrin alpha V was immunoprecipitated from 1 mg of A549 (Human lung carcinoma) whole cell extract with ab179475 at 1/40 dilution. Western blot analysis was performed from the immunoprecipitate using ab179475 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

Lane 1 : A549 whole cell extract.

Lane 2 : PBS instead of A549 whole cell extract.

Blocking/Dilution buffer : 5% NFDM/TBST.

ab179475 can recognize 135kDa full length Integrin alpha V and 125kDa heavy chain. The 125 kDa band is Integrin alpha V heavy chain.

All lanes:

Immunoprecipitation - Anti-Integrin alpha V antibody [EPR16800] (ab179475)

Predicted band size: 116 kDa

Observed band size: 125 kDa

false

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha V antibody [EPR16800] (AB179475)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Integrin alpha V with ab179475 at 1/500 dilution followed by Goat Anti-Rabbit HRP (IgG H&L) (ab97051) secondary at 1/500 dilution.

Membrane and cytoplasm staining on mouse kidney tubule and glomerulus is observed. Counterstained with hematoxylin.

Negative control : Using PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• WB

Lab

Western blot - Anti-Integrin alpha V antibody [EPR16800] (AB179475)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475) at 1/1000 dilution

All lanes:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate treated with PNGase F. at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 116 kDa

Observed band size: 116 kDa,135 kDa

false

Exposure time: 20s

• WB

Lab

Western blot - Anti-Integrin alpha V antibody [EPR16800] (AB179475)

Western blot : Anti-Integrin alpha V antibody [EPR16800] ab179475 staining at 1/5000 dilution, shown in green; Mouse anti alpha Tubulin ab7291 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 116 kDa in Wild-type HCT 116 cell lysates with no signal observed at this size in ITGAV knockout HCT 116 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 100pc LICOR in TBS-0.1 % Tween 20 (TBS-T) before incubation with primary antibodies overnight at 4 C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

Lanes 1 - 4:

Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475) at 1/5000 dilution

Lanes 1 - 4:

Western blot - Anti-Integrin alpha V antibody [EPR16800] - Low endotoxin, Azide free (<a href='/en-us/products/primary-antibodies/integrin-alpha-v-antibody-epr16800-low-endotoxin-azide-free-ab222222'>ab222222</a>) at 1/5000 dilution

Lanes 1 - 4:

Western blot - Anti-Integrin alpha V antibody [EPR16800] - BSA and Azide free (Detector) (<a href='/en-us/products/primary-antibodies/integrin-alpha-v-antibody-epr16800-bsa-and-azide-free-detector-ab245051'>ab245051</a>) at 1/5000 dilution

Lane 1:

Wild-type HCT 116 at 20 µg

Lane 2:

Western blot - Human ITGAV knockout HCT116 cell line (<a href='/en-us/products/cell-lines/human-itgav-knockout-hct116-cell-line-ab286702'>ab286702</a>) at 20 µg

Lane 3:

A549 at 20 µg

Lane 4:

Daudi at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 116 kDa

Observed band size: 116 kDa

false

• WB

Lab

Western blot - Anti-Integrin alpha V antibody [EPR16800] (AB179475)

In Western blot, Anti-Integrin alpha V antibody [EPR16800] was used as membrane Control (ab179475) at 1 : 5,000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Kir2.1/KCNJ2 antibody [EPR4530] (<a href='/en-us/products/primary-antibodies/kir21-kcnj2-antibody-epr4530-ab109750'>ab109750</a>) at 1/1000 dilution

Lane 1:

HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 2:

HEK-293 membrane lysate at 20 µg

Lane 3:

HEK-293 cytoplasm lysate at 20 µg

Lane 4:

HEK-293 nuclear lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 55 kDa

false

Exposure time: 20s

• WB

Supplier Data

Western blot - Anti-Integrin alpha V antibody [EPR16800] (AB179475)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

All lanes:

Western blot - Anti-RANK antibody [EPR26196-15] (<a href='/en-us/products/primary-antibodies/rank-antibody-epr26196-15-ab305233'>ab305233</a>) at 1/1000 dilution

Lane 1:

K-562 (human chronic myelogenous leukemia lymphoblast) non-membrane fraction at 20 µg

Lane 2:

K-562 membrane fraction at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 80 kDa

true

Exposure time: 3min

• WB

Supplier Data

Western blot - Anti-Integrin alpha V antibody [EPR16800] (AB179475)

Blocking/dilution buffer : 5% NFDM/TBST.

ab179475 can recognize 135kDa full length Integrin alpha V and 125kDa heavy chain. The 125 kDa band is Integrin alpha V heavy chain.

All lanes:

Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475) at 1/50000 dilution

Lane 1:

HUVEC (Human umbilical vein endothelial cell line) whole cell lysates at 20 µg

Lane 2:

HT-29 (Human colorectal adenocarcinoma cells) whole cell lysates at 20 µg

Lane 3:

A549 (Human lung carcinoma) whole cell lysates at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 116 kDa

Observed band size: 125 kDa

false

• WB

Lab

Western blot - Anti-Integrin alpha V antibody [EPR16800] (AB179475)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475) at 1/1000 dilution

Lane 1:

Untreated K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate. at 15 µg

Lane 2:

K-562 (Human chronic myelogenous leukemia lymphoblast) treated with PNGase F whole cell lysate. at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 116 kDa

Observed band size: 116 kDa,135 kDa

false

Exposure time: 80s

• WB

Supplier Data

Western blot - Anti-Integrin alpha V antibody [EPR16800] (AB179475)

Blocking/dilution buffer : 5% NFDM/TBST.

ab179475 can recognize 135kDa full length Integrin alpha V and 125kDa heavy chain. The 125 kDa band is Integrin alpha V heavy chain.

All lanes:

Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475) at 1/5000 dilution

Lane 1:

Human fetal kidney lysates at 10 µg

Lane 2:

Human fetal brain lysates at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 116 kDa

Observed band size: 125 kDa

false

• WB

Supplier Data

Western blot - Anti-Integrin alpha V antibody [EPR16800] (AB179475)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The identity of the higher MW band at approximately 50 kDa (in lane 2) is unknown.

Samples are non-boiled as boiling may cause protein aggregation.

In Western blot, Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (ab176842) staining at 1/100000 dilution.

In Western blot, Anti-Integrin alpha V antibody [EPR16800] (ab179475) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-GPCR GPRC5D antibody [EPR28376-41] (<a href='/en-us/products/primary-antibodies/gpcr-gprc5d-antibody-epr28376-41-ab315808'>ab315808</a>) at 1/1000 dilution

Lane 1:

RPMI-8226 (human plasmacytoma, myeloma B lymphocyte) membrane fraction at 50 µg

Lane 2:

RPMI-8226 nuclear fraction at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 33 kDa,125 kDa,15 kDa

false

Exposure time: 15s

• WB

AbReview63958****

Western blot - Anti-Integrin alpha V antibody [EPR16800] (AB179475)

Diluent : 2% BSA in 1X TBST.

Blocked using 5% BSA for 1 hour at RT.

All lanes:

Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475) at 1/2000 dilution

All lanes:

Mouse skin whole cell lysate at 40 µg

Secondary

All lanes:

Goat anti-Rabbit IgG(H+L)-HRP at 1/5000 dilution

Predicted band size: 116 kDa

true

Exposure time: 25s

This image is courtesy of an anonymous Abreview.

• WB

Lab

Western blot - Anti-Integrin alpha V antibody [EPR16800] (AB179475)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID : 28895560).

Samples are non-boiled as boiling may cause protein aggregation.

This blot was developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) (1 : 10000) (124KDa); Anti-Integrin alpha V antibody [EPR16800](1 : 5000)(125/135KDa).

All lanes:

Western blot - Anti-FLT3 antibody [EPR31042-551] (<a href='/en-us/products/primary-antibodies/flt3-antibody-epr31042-551-ab326101'>ab326101</a>) at 1/1000 dilution

Lane 1:

Mouse brain membrane fraction at 48 µg

Lane 2:

Mouse brain total tissue lysate at 48 µg

Lane 3:

Mouse thymus membrane fraction at 48 µg

Lane 4:

Mouse thymus total tissue lysate at 48 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 130-160 kDa,124 kDa,125-135 kDa

true

Exposure time: 180s

• WB

Lab

Western blot - Anti-Integrin alpha V antibody [EPR16800] (AB179475)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. ab181602 and ab179475 were used as loading controls. The molecular weight observed is consistent with literatures (PMID : 9722601, PMID : 3360311). Samples are non-boiled as boiling may cause protein aggregation and were run on a Bis-Tris gel. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution and anti- Integrin alpha V antibody (ab179475) loading control staining at 1/5000 dilution.

All lanes:

Western blot - Anti-CaSR antibody [EPR24050-59] (<a href='/en-us/products/primary-antibodies/casr-antibody-epr24050-59-ab259846'>ab259846</a>) at 1/1000 dilution

Lane 1:

Mouse kidney membrane tissue lysate at 10 µg

Lane 2:

Mouse kidney membrane control tissue lysate at 10 µg

Lane 3:

Rat kidney membrane tissue lysate at 10 µg

Lane 4:

Rat skeletal muscle tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

false

Exposure time: 10s

• WB

Supplier Data

Western blot - Anti-Integrin alpha V antibody [EPR16800] (AB179475)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The identity of the bands higher than 150 kDa and lower than 50 kDa are unknown.

In Western blot, Anti-Integrin alpha V antibody [EPR16800] (ab179475) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-FRS2 antibody [EPR28714-126] (<a href='/en-us/products/primary-antibodies/frs2-antibody-epr28714-126-ab320727'>ab320727</a>) at 1/1000 dilution

Lane 1:

F9 (mouse embryonal carcinoma epithelial cell) non-membrane fraction at 20 µg

Lane 2:

F9 (mouse embryonal carcinoma epithelial cell) membrane fraction at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 74 kDa,116 kDa,135 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-Integrin alpha V antibody [EPR16800] (AB179475)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : HL-1.

Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

Alternatively spliced forms of the EVI-1 gene encode at least three distinct proteins : EVI-1 (145 kDa), MDS1/EVI-1 (200 kDa) and EVI-1Δ324 (88 kDa) PMID : (PMID : 24944602).

The identity of the bands between 75 kDa and 180 kDa are unknown.

In Western blot, Anti-Integrin alpha V antibody [EPR16800] (ab179475) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-EVI1 antibody [EPR26816-18] (<a href='/en-us/products/primary-antibodies/evi1-antibody-epr26816-18-ab315973'>ab315973</a>) at 1/1000 dilution

Lane 1:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 80 µg

Lane 2:

HL-1 (mouse atrial muscle cell) whole cell lysate at 80 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 200 kDa,125 kDa

false

Exposure time: 59s

• WB

Supplier Data

Western blot - Anti-Integrin alpha V antibody [EPR16800] (AB179475)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative controls : kidney (PMID : 24309898), cerebellum (PMID : 24309898). In Western blot, anti- Integrin alpha V antibody (ab179475) loading control staining at 1/5000 dilution. Exposure time : 180 seconds

All lanes:

Western blot - Anti-Neutrophil Elastase antibody [EPR28386-66] (<a href='/en-us/products/primary-antibodies/neutrophil-elastase-antibody-epr28386-66-ab310335'>ab310335</a>) at 1/1000 dilution

Lane 1:

Rat bone marrow tissue lysate at 20 µg

Lane 2:

Rat cerebellum tissue lysate at 20 µg

Lane 3:

Rat kidney tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-Integrin alpha V antibody [EPR16800] (AB179475)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Kir4.1/KCNJ10 is expressed in vivo as a homotetramer (four units of KIR4.1) or a heterotetramer with KIR5.1(two units of KIR4.1 and KIR5.1). (PMID : 33348803; PMID : 15310750 ). This antibody can only detect the tetramer of Kir4.1/KCNJ10. Samples are non-boiled as boiling may cause protein aggregation. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Anti-Integrin alpha V (ab179475) control staining at 1/1000 dilution Exposure time : 15 seconds

All lanes:

Western blot - Anti-Kir4.1/KCNJ10 antibody [EPR27251-74] (<a href='/en-us/products/primary-antibodies/kir41-kcnj10-antibody-epr27251-74-ab312844'>ab312844</a>) at 1/1000 dilution

Lane 1:

Mouse brain non-membrane fraction at 20 µg

Lane 2:

Mouse brain membrane fraction at 20 µg

Lane 3:

Rat brain non-membrane fraction at 20 µg

Lane 4:

Rat brain membrane fraction at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 250 kDa

false

Exposure time: 15s

• WB

Supplier Data

Western blot - Anti-Integrin alpha V antibody [EPR16800] (AB179475)

Blocking and diluting buffer and concentration : 5% NFDM/TBST The identity of the bands between 75 kDa and 25 kDa are unknown. In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution. In Western blot, Anti-Integrin alpha V antibody [EPR16800] (ab179475) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-GluN2D antibody [EPR28378-56] (<a href='/en-us/products/primary-antibodies/glun2d-antibody-epr28378-56-ab314664'>ab314664</a>) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse brain membrane fraction at 20 µg

Lane 3:

Mouse brain non-membrane fraction at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 165 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-Integrin alpha V antibody [EPR16800] (AB179475)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID : 29285063, 19726871).

Samples are non-boiled as boiling may cause protein aggregation.

In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Anti-Integrin alpha V (ab179475) control staining at 1/5000 dilution.

All lanes:

Western blot - Anti-Cx40/GJA5 antibody [EPR28370-62] (<a href='/en-us/products/primary-antibodies/cx40-gja5-antibody-epr28370-62-ab313644'>ab313644</a>) at 1/1000 dilution

Lane 1:

Mouse lung non-membrane fraction at 20 µg

Lane 2:

Mouse lung membrane fraction at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 40 kDa,36 kDa

false

Exposure time: 37s

• WB

Lab

Western blot - Anti-Integrin alpha V antibody [EPR16800] (AB179475)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, anti-GAPDH antibody (ab181602) staining at 1/200,000 dilution and anti-Integrin alpha V (ab179475) staining at 1/5,000 dilution.

Samples are non-boiled as boiling may cause protein aggregation.

All lanes:

Western blot - Anti-Noradrenaline transporter antibody [EPR23527-534] (<a href='/en-us/products/primary-antibodies/noradrenaline-transporter-antibody-epr23527-534-ab254361'>ab254361</a>) at 1/1000 dilution

Lane 1:

Mouse brain membrane tissue lysate at 20 µg

Lane 2:

Mouse membrane-depleted tissue lysate at 20 µg

Lane 3:

Rat brain membrane tissue lysate at 20 µg

Lane 4:

Rat membrane-depleted tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 69 kDa

Observed band size: 69 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-Integrin alpha V antibody [EPR16800] (AB179475)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression of TARC/CCL17 is upregulated in response to M-CSF, GM-CSF and BFA treatment (PMID : 27525438).

In Western blot, Anti-Integrin alpha V antibody [EPR16800] staining at 1/5000 dilution.

All lanes:

Western blot - Anti-TARC/CCL17 antibody [EPR28961-9] (<a href='/en-us/products/primary-antibodies/tarc-ccl17-antibody-epr28961-9-ab323800'>ab323800</a>) at 1/1000 dilution

Lane 1:

Untreated Mouse bone marrow lysate at 20 µg

Lane 2:

Mouse bone marrow treated with 100ng/mL M-CSF for 7 days, 20ng/ml GM-CSF and 300ng/ml BFA for 16 hours lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 11 kDa,125 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-Integrin alpha V antibody [EPR16800] (AB179475)

Blocking/dilution buffer : 5% NFDM/TBST.

ab179475 can recognize 135kDa full length Integrin alpha V and 125kDa heavy chain. The 125 kDa band is Integrin alpha V heavy chain.

All lanes:

Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475) at 1/5000 dilution

Lane 1:

Mouse brain lysates at 10 µg

Lane 2:

Mouse kidney lysates at 10 µg

Lane 3:

Mouse spleen lysates at 10 µg

Lane 4:

Rat brain lysates at 10 µg

Lane 5:

Rat kidney lysates at 10 µg

Lane 6:

C6 (Rat glial tumor cells) whole cell lysates at 10 µg

Lane 7:

NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates at 10 µg

Lane 8:

Raji (Human Burkitt's lymphoma cell line) whole cell lysates at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 116 kDa

Observed band size: 125 kDa,135 kDa

false