AB124968

Anti-Integrin alpha V antibody [EPR5583]

Name: Anti-Integrin alpha V antibody [EPR5583] (ab124968) | Abcam
Brand: Abcam Limited
SKU: AB124968
Price: 210 USD
Availability: InStock
Rating: 4 (2 reviews)

20ul selling size
RabMAb
Recombinant
What is this?

(2 Reviews)

(12 Publications)

Rabbit Recombinant Monoclonal Integrin alpha V antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 12 publications.

View Alternative Names

CD51, MSK8, VNRA, VTNR, ITGAV, Integrin alpha-V, Vitronectin receptor, Vitronectin receptor subunit alpha

1 Images

Lab

Western blot - Anti-Integrin alpha V antibody [EPR5583] (AB124968)

The bands detected are consistent with what have been described in the literatures (PMID : 11741954, 27033701). Full-length Integrin alpha V : 150kDa; Integrin alpha V light chain : 27-30kDa

All lanes:

Western blot - Anti-Integrin alpha V antibody [EPR5583] (ab124968) at 1/1000 dilution

Lane 1:

A549 (Human lung carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

HUVEC (Human umbilical vein endothelial cell) whole cell lysate at 20 µg

Lane 4:

U-87 MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysate at 20 µg

Lane 5:

NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 6:

C6 (Rat glial tumor glial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 116 kDa

Observed band size: 150 kDa,27-30 kDa

false

Carrier free

Anti-Integrin alpha V antibody [EPR5583] - BSA and Azide free (Capture)
Carrier free

Anti-Integrin alpha V antibody [EPR5583] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5583

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Integrin alpha V also known as integrin av CD51 and CD51/CD61 is a transmembrane protein with an approximate mass of 125 kDa. It functions mechanically by mediating cell adhesion and signaling through its interaction with the extracellular matrix and other cells. Integrin alpha V is widely expressed in a variety of tissues including endothelial and epithelial cells as well as some tumor cells. This protein forms heterodimers typically with beta subunits like CD61 which is also referred to as beta 3 integrin to execute its biological functions.

Biological function summary

Integrin alpha V plays a pivotal role in cellular processes such as migration proliferation and survival. It participates in the formation of multi-molecular complexes within cellular structures. The integrin av integrins particularly in conjunction with its CD51/CD61 form contribute to the regulation of angiogenesis immune response and wound healing. These functions arise from the integrin’s ability to act as a receptor for numerous ligands including fibrinogen fibronectin and vitronectin.

Pathways

Integrin alpha V is a critical component of the integrin signaling and focal adhesion pathways. These pathways involve a network of interactions that include proteins like focal adhesion kinase (FAK) and Src family kinases. Integrin alpha V’s interaction within these pathways assists in transmitting mechanical signals from the extracellular environment to the intracellular signaling cascades affecting cytoskeleton dynamics and gene expression. The pathway interactions with integrin av also coordinate complex signaling with other receptors and matrix proteins like laminins enhancing cellular communication and response to environmental changes.

Integrin alpha V’s dysregulation associates with conditions such as cancer and fibrosis. Overexpression or aberrant functioning of integrin av contributes to tumor progression and metastasis by promoting angiogenesis and cell migration. In fibrotic diseases the protein's interactions with the extracellular matrix components are altered leading to excessive tissue scarring and dysfunction. Furthermore integrin alpha V interplays with proteins such as matrix metalloproteinases (MMPs) in the tumor microenvironment facilitating tissue remodeling and cancer cell invasion.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

The alpha-V (ITGAV) integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. ITGAV : ITGB3 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling (PubMed : 23125415). ITGAV : ITGB3 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed : 20682778). ITGAV : ITGB3 binds to FGF1 and this binding is essential for FGF1 signaling (PubMed : 18441324). ITGAV : ITGB3 binds to FGF2 and this binding is essential for FGF2 signaling (PubMed : 28302677). ITGAV : ITGB3 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed : 19578119). ITGAV : ITGB3 binds to IGF2 and this binding is essential for IGF2 signaling (PubMed : 28873464). ITGAV : ITGB3 binds to IL1B and this binding is essential for IL1B signaling (PubMed : 29030430). ITGAV : ITGB3 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed : 18635536, PubMed : 25398877). ITGAV : ITGB3 and ITGAV : ITGB6 act as receptors for fibrillin-1 (FBN1) and mediate R-G-D-dependent cell adhesion to FBN1 (PubMed : 12807887, PubMed : 17158881). Integrin alpha-V/beta-6 or alpha-V/beta-8 (ITGAV : ITGB6 or ITGAV : ITGB8) mediates R-G-D-dependent release of transforming growth factor beta-1 (TGF-beta-1) from regulatory Latency-associated peptide (LAP), thereby playing a key role in TGF-beta-1 activation (PubMed : 15184403, PubMed : 22278742, PubMed : 28117447). ITGAV : ITGB3 acts as a receptor for CD40LG (PubMed : 31331973). ITGAV : ITGB3 acts as a receptor for IBSP and promotes cell adhesion and migration to IBSP (PubMed : 10640428).. (Microbial infection) Integrin ITGAV : ITGB5 acts as a receptor for Adenovirus type C.. (Microbial infection) Integrin ITGAV : ITGB5 and ITGAV : ITGB3 act as receptors for Coxsackievirus A9 and B1.. (Microbial infection) Integrin ITGAV : ITGB3 acts as a receptor for Herpes virus 8/HHV-8.. (Microbial infection) Integrin ITGAV : ITGB6 acts as a receptor for herpes simplex 1/HHV-1.. (Microbial infection) Integrin ITGAV : ITGB3 acts as a receptor for Human parechovirus 1.. (Microbial infection) Integrin ITGAV : ITGB3 acts as a receptor for West nile virus.. (Microbial infection) In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.

See full target information ITGAV

style

left-column

Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 44:115319 PubMed39964812

2025

Luminescent sensing of conformational integrin activation in living cells.

Applications

Unspecified application

Species

Unspecified reactive species

Giulia Villari,Noemi Gioelli,Marta Gino,Heng Zhang,Kelly Hodge,Francesca Cordero,Sara Zanivan,Jieqing Zhu,Guido Serini

Signal transduction and targeted therapy 7:290 PubMed36042225

2022

p53 positively regulates the proliferation of hepatic progenitor cells promoted by laminin-521.

Applications

Unspecified application

Species

Unspecified reactive species

Mingyang Ma,Shuyao Hua,Xiangde Min,Liang Wang,Jun Li,Ping Wu,Huifang Liang,Bixiang Zhang,Xiaoping Chen,Shuai Xiang

PLoS pathogens 18:e1010798 PubMed36007070

2022

An Arginine-Rich Motif in the ORF2 capsid protein regulates the hepatitis E virus lifecycle and interactions with the host cell.

Applications

Unspecified application

Species

Unspecified reactive species

Kévin Hervouet,Martin Ferrié,Maliki Ankavay,Claire Montpellier,Charline Camuzet,Virginie Alexandre,Aïcha Dembélé,Cécile Lecoeur,Arnold Thomas Foe,Peggy Bouquet,David Hot,Thibaut Vausselin,Jean-Michel Saliou,Sophie Salomé-Desnoulez,Alexandre Vandeputte,Laurent Marsollier,Priscille Brodin,Marlène Dreux,Yves Rouillé,Jean Dubuisson,Cécile-Marie Aliouat-Denis,Laurence Cocquerel

Scientific reports 11:4217 PubMed33602965

2021

Integrins mediate placental extracellular vesicle trafficking to lung and liver in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Sean L Nguyen,Soo Hyun Ahn,Jacob W Greenberg,Benjamin W Collaer,Dalen W Agnew,Ripla Arora,Margaret G Petroff

Journal of dental research 100:479-486 PubMed33179547

2020

Nuclear F-actin Cytology in Oral Epithelial Dysplasia and Oral Squamous Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

M P McRae,A R Kerr,M N Janal,M H Thornhill,S W Redding,N Vigneswaran,S K Kang,R Niederman,N J Christodoulides,D A Trochesset,C Murdoch,I Dapkins,J Bouquot,S S Modak,G W Simmons,J T McDevitt

PLoS pathogens 16:e1008881 PubMed33002094

2020

Periodontal pathogens promote cancer aggressivity via TLR/MyD88 triggered activation of Integrin/FAK signaling that is therapeutically reversible by a probiotic bacteriocin.

Applications

Unspecified application

Species

Unspecified reactive species

Pachiyappan Kamarajan,Islam Ateia,Jae M Shin,J Christopher Fenno,Charles Le,Ling Zhan,Ana Chang,Richard Darveau,Yvonne L Kapila

International journal of molecular sciences 21: PubMed32630820

2020

Effects of DSPP and MMP20 Silencing on Adhesion, Metastasis, Angiogenesis, and Epithelial-Mesenchymal Transition Proteins in Oral Squamous Cell Carcinoma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jaya Aseervatham,Kalu U E Ogbureke

Cell stem cell 26:187-204.e10 PubMed31956038

2020

Zika Virus Targets Glioblastoma Stem Cells through a SOX2-Integrin αβ Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhe Zhu,Pinar Mesci,Jean A Bernatchez,Ryan C Gimple,Xiuxing Wang,Simon T Schafer,Hiromi I Wettersten,Sungjun Beck,Alex E Clark,Qiulian Wu,Briana C Prager,Leo J Y Kim,Rekha Dhanwani,Sonia Sharma,Alexandra Garancher,Sara M Weis,Stephen C Mack,Priscilla D Negraes,Cleber A Trujillo,Luiz O Penalva,Jing Feng,Zhou Lan,Rong Zhang,Alex W Wessel,Sanjay Dhawan,Michael S Diamond,Clark C Chen,Robert J Wechsler-Reya,Fred H Gage,Hongzhen Hu,Jair L Siqueira-Neto,Alysson R Muotri,David A Cheresh,Jeremy N Rich

Journal of cell science 131: PubMed29142101

2018

Loss of ADAM9 expression impairs β1 integrin endocytosis, focal adhesion formation and cancer cell migration.

Applications

Unspecified application

Species

Unspecified reactive species

Kasper J Mygind,Jeanette Schwarz,Pranshu Sahgal,Johanna Ivaska,Marie Kveiborg

Acta biomaterialia 49:260-271 PubMed27919840

2016

Development, fabrication and evaluation of a novel biomimetic human breast tissue derived breast implant surface.

Applications

Unspecified application

Species

Unspecified reactive species

S Barr,E W Hill,A Bayat

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Integrin alpha V antibody [EPR5583]

Western blot - Anti-Integrin alpha V antibody [EPR5583] (AB124968)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (12)

Product promise