AB63490

Anti-Integrin alpha V antibody [RMV-7]

Name: Anti-Integrin alpha V antibody [RMV-7] (ab63490) | Abcam
Brand: Abcam Limited
SKU: AB63490
Price: 515 USD
Availability: InStock

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(4 Publications)

Rat Monoclonal Integrin alpha V antibody. Suitable for IP, Flow Cyt and reacts with Mouse samples. Cited in 4 publications. Immunogen corresponding to Cell preparation containing Itgav protein.

View Alternative Names

CD51, Integrin alpha-V, Vitronectin receptor subunit alpha, Itgav

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

RMV-7

Isotype

IgG1

Carrier free

Reacts with

Mouse

Applications

Flow Cyt, IP

applications

Immunogen

Cell preparation containing Itgav protein. The exact immunogen used to generate this antibody is proprietary information.

P43406

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "1/10 - 1/100", "FlowCyt-species-notes": "<a href='/en-us/products/unavailable/rat-igg1-kappa-rtk2071-isotype-control-low-endotoxin-azide-free-ab18407'>ab18407</a> - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.Use 10 μl of the suggested working dilution to label 106 cells in 100μl." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.

Storage buffer

pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Integrin alpha V also known as integrin av CD51 and CD51/CD61 is a transmembrane protein with an approximate mass of 125 kDa. It functions mechanically by mediating cell adhesion and signaling through its interaction with the extracellular matrix and other cells. Integrin alpha V is widely expressed in a variety of tissues including endothelial and epithelial cells as well as some tumor cells. This protein forms heterodimers typically with beta subunits like CD61 which is also referred to as beta 3 integrin to execute its biological functions.

Biological function summary

Integrin alpha V plays a pivotal role in cellular processes such as migration proliferation and survival. It participates in the formation of multi-molecular complexes within cellular structures. The integrin av integrins particularly in conjunction with its CD51/CD61 form contribute to the regulation of angiogenesis immune response and wound healing. These functions arise from the integrin’s ability to act as a receptor for numerous ligands including fibrinogen fibronectin and vitronectin.

Pathways

Integrin alpha V is a critical component of the integrin signaling and focal adhesion pathways. These pathways involve a network of interactions that include proteins like focal adhesion kinase (FAK) and Src family kinases. Integrin alpha V’s interaction within these pathways assists in transmitting mechanical signals from the extracellular environment to the intracellular signaling cascades affecting cytoskeleton dynamics and gene expression. The pathway interactions with integrin av also coordinate complex signaling with other receptors and matrix proteins like laminins enhancing cellular communication and response to environmental changes.

Integrin alpha V’s dysregulation associates with conditions such as cancer and fibrosis. Overexpression or aberrant functioning of integrin av contributes to tumor progression and metastasis by promoting angiogenesis and cell migration. In fibrotic diseases the protein's interactions with the extracellular matrix components are altered leading to excessive tissue scarring and dysfunction. Furthermore integrin alpha V interplays with proteins such as matrix metalloproteinases (MMPs) in the tumor microenvironment facilitating tissue remodeling and cancer cell invasion.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The alpha-V (ITGAV) integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, TGFB1 and vWF (PubMed : 10025398, PubMed : 9827803). They recognize the sequence R-G-D in a wide array of ligands. Alpha-V integrins may play a role in embryo implantation, angiogenesis and wound healing (PubMed : 9827803). ITGAV : ITGB3 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling (By similarity). ITGAV : ITGB3 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling. ITGAV : ITGB3 binds to FGF1 and this binding is essential for FGF1 signaling (By similarity). ITGAV : ITGB3 binds to FGF2 and this binding is essential for FGF2 signaling (By similarity). ITGAV : ITGB3 binds to IGF1 and this binding is essential for IGF1 signaling (By similarity). ITGAV : ITGB3 binds to IGF2 and this binding is essential for IGF2 signaling (By similarity). ITGAV : ITGB3 binds to IL1B and this binding is essential for IL1B signaling (By similarity). ITGAV : ITGB3 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (By similarity). ITGAV : ITGB3 and ITGAV : ITGB6 act as a receptor for fibrillin-1 (FBN1) and mediate R-G-D-dependent cell adhesion to FBN1 (By similarity). Integrin alpha-V/beta-6 or alpha-V/beta-8 (ITGAV : ITGB6 or ITGAV : ITGB8) mediates R-G-D-dependent release of transforming growth factor beta-1 (TGF-beta-1) from regulatory Latency-associated peptide (LAP), thereby playing a key role in TGF-beta-1 activation (PubMed : 10025398, PubMed : 25127859). ITGAV : ITGB3 acts as a receptor for CD40LG (By similarity). ITGAV : ITGB3 binds to the Lilrb4a/Gp49b receptor and enhances the Lilrb4a-mediated inhibition of mast cell activation (PubMed : 11323698). ITGAV : ITGB3 also suppresses marginal zone B cell antibody production through its interaction with Lilrb4a (PubMed : 24935931). ITGAV : ITGB3 acts as a receptor for IBSP and promotes cell adhesion and migration to IBSP (By similarity).

See full target information Itgav

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

PloS one 20:e0331564 PubMed40892758

2025

A cytotoxic peptide-drug conjugate for tumor-specific delivery of co-injected molecules.

Applications

Unspecified application

Species

Unspecified reactive species

Norio Miyamura,Chisato M Yamazaki,Yasuaki Anami,Kyoji Tsuchikama,Kazuki N Sugahara

Cell reports 42:113038 PubMed37624696

2023

Osteopontin drives retinal ganglion cell resiliency in glaucomatous optic neuropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Mengya Zhao,Kenichi Toma,Benyam Kinde,Liang Li,Amit K Patel,Kong-Yan Wu,Matthew R Lum,Chengxi Tan,Jody E Hooper,Arnold R Kriegstein,Anna La Torre,Yaping Joyce Liao,Derek S Welsbie,Yang Hu,Ying Han,Xin Duan

Nature medicine 20:175-83 PubMed24441829

2014

Mfge8 promotes obesity by mediating the uptake of dietary fats and serum fatty acids.

Applications

Unspecified application

Species

Unspecified reactive species

Amin Khalifeh-Soltani,William McKleroy,Stephen Sakuma,Yuk Yin Cheung,Kevin Tharp,Yifu Qiu,Scott M Turner,Ajay Chawla,Andreas Stahl,Kamran Atabai

The Journal of cell biology 198:657-76 PubMed22908313

2012

Mena binds α5 integrin directly and modulates α5β1 function.

Applications

Species

Unspecified reactive species

Stephanie L Gupton,Daisy Riquelme,Shannon K Hughes-Alford,Jenny Tadros,Shireen S Rudina,Richard O Hynes,Douglas Lauffenburger,Frank B Gertler

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Integrin alpha V antibody [RMV-7]

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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