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AB20143

Anti-Integrin alpha V beta 3 antibody [23C6]

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(8 Publications)

Mouse Monoclonal Integrin alpha V antibody. Suitable for IP, Flow Cyt, FuncS (Neut/Block), IHC-Fr and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Cell preparation containing ITGAV protein.

View Alternative Names

CD51, MSK8, VNRA, VTNR, ITGAV, Integrin alpha-V, Vitronectin receptor, Vitronectin receptor subunit alpha

  • 519 FITC

    FITC Anti-Integrin alpha V beta 3 antibody [23C6]

  • Biotin

    Biotin Anti-Integrin alpha V beta 3 antibody [23C6]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

23C6

Isotype

IgG1

Light chain type

unknown

Carrier free

No

Reacts with

Human

Applications

IP, FuncS (Neut/Block), Flow Cyt, IHC-Fr

applications

Immunogen

Cell preparation containing ITGAV protein. The exact immunogen used to generate this antibody is proprietary information.

P06756

Specificity

Reacts with Integrin Alpha V Beta 3. 23C6 may be useful for bone resorption modulation, osteoclast identification, receptor purification, malignant melanoma identification and treatment.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "FuncSNeutBlock" : {"fullname" : "Functional Studies (Neut/Block)", "shortname":"FuncS (Neut/Block)"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "FuncSNeutBlock-species-checked": "guaranteed", "FuncSNeutBlock-species-dilution-info": "", "FuncSNeutBlock-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p>Samples should be acetone fixed.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Integrin alpha V beta 3 also known as integrin αvβ3 or avb3 integrin is a transmembrane receptor with a molecular weight of about 140 kDa. This protein is composed of the alpha V (αv) and beta 3 (β3) subunits. Integrin αvβ3 is widely expressed in various cell types including endothelial cells osteoclasts and some tumor cells. Its function involves mechanical interaction with the extracellular matrix (ECM) allowing cells to adhere to ECM proteins such as vitronectin and fibronectin. This integrin plays an important role in cell adhesion migration and survival.
Biological function summary

Integrin alpha V beta 3 engages in various cellular processes by serving as a part of integrin complexes. It mediates signal transduction from the ECM to the cell interior influencing cellular responses such as shape changes survival and proliferation. The receptor regulates angiogenesis which is the formation of new blood vessels and osteoclast-mediated bone resorption. These functions are essential for tissue remodeling and repair. The integrin can also modulate immune responses by interacting with immune cells affecting inflammation and wound healing processes.

Pathways

Integrin alpha V beta 3 is an important component in signaling pathways such as the PI3K/AKT and MAPK pathways. These pathways facilitate the integrin's role in cell migration growth and survival. It interacts with proteins like focal adhesion kinase (FAK) and SRC kinase bridging signals from the ECM to intracellular signaling cascades. The integrin's ability to bind ligands and initiate these pathways makes it an important player in cellular responses to the extracellular environment influencing how cells align mechanically and biochemically.

Integrin alpha V beta 3 has significant associations with cancer progression and osteoporosis. In various cancers including melanoma and breast cancer overexpression of this integrin supports tumor angiogenesis and metastasis connecting its role with angiogenic growth factors and matrix metalloproteinases. In osteoporosis it collaborates with proteins like osteopontin promoting osteoclast attachment to the bone matrix thereby influencing bone degradation. Understanding its role in these disorders highlights potential therapeutic targets for inhibiting unwanted angiogenesis and excessive bone resorption.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The alpha-V (ITGAV) integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. ITGAV : ITGB3 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling (PubMed : 23125415). ITGAV : ITGB3 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed : 20682778). ITGAV : ITGB3 binds to FGF1 and this binding is essential for FGF1 signaling (PubMed : 18441324). ITGAV : ITGB3 binds to FGF2 and this binding is essential for FGF2 signaling (PubMed : 28302677). ITGAV : ITGB3 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed : 19578119). ITGAV : ITGB3 binds to IGF2 and this binding is essential for IGF2 signaling (PubMed : 28873464). ITGAV : ITGB3 binds to IL1B and this binding is essential for IL1B signaling (PubMed : 29030430). ITGAV : ITGB3 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed : 18635536, PubMed : 25398877). ITGAV : ITGB3 and ITGAV : ITGB6 act as receptors for fibrillin-1 (FBN1) and mediate R-G-D-dependent cell adhesion to FBN1 (PubMed : 12807887, PubMed : 17158881). Integrin alpha-V/beta-6 or alpha-V/beta-8 (ITGAV : ITGB6 or ITGAV : ITGB8) mediates R-G-D-dependent release of transforming growth factor beta-1 (TGF-beta-1) from regulatory Latency-associated peptide (LAP), thereby playing a key role in TGF-beta-1 activation (PubMed : 15184403, PubMed : 22278742, PubMed : 28117447). ITGAV : ITGB3 acts as a receptor for CD40LG (PubMed : 31331973). ITGAV : ITGB3 acts as a receptor for IBSP and promotes cell adhesion and migration to IBSP (PubMed : 10640428).. (Microbial infection) Integrin ITGAV : ITGB5 acts as a receptor for Adenovirus type C.. (Microbial infection) Integrin ITGAV : ITGB5 and ITGAV : ITGB3 act as receptors for Coxsackievirus A9 and B1.. (Microbial infection) Integrin ITGAV : ITGB3 acts as a receptor for Herpes virus 8/HHV-8.. (Microbial infection) Integrin ITGAV : ITGB6 acts as a receptor for herpes simplex 1/HHV-1.. (Microbial infection) Integrin ITGAV : ITGB3 acts as a receptor for Human parechovirus 1.. (Microbial infection) Integrin ITGAV : ITGB3 acts as a receptor for West nile virus.. (Microbial infection) In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
See full target information ITGAV
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Blood 116:3554-63 PubMed20664052

2010

A novel role of IL-17-producing lymphocytes in mediating lytic bone disease in multiple myeloma.

Applications

Unspecified application

Species

Unspecified reactive species

Kimberly Noonan,Luigi Marchionni,Judy Anderson,Drew Pardoll,G David Roodman,Ivan Borrello

Biomaterials 30:1089-97 PubMed19027948

2008

Controlling integrin specificity and stem cell differentiation in 2D and 3D environments through regulation of fibronectin domain stability.

Applications

Unspecified application

Species

Unspecified reactive species

Mikaël M Martino,Mayumi Mochizuki,Dominique A Rothenfluh,Sandra A Rempel,Jeffrey A Hubbell,Thomas H Barker

Journal of cell science 121:2696-704 PubMed18653538

2008

Fibrillin-1 microfibril deposition is dependent on fibronectin assembly.

Applications

Unspecified application

Species

Unspecified reactive species

Rachel Kinsey,Matthew R Williamson,Shazia Chaudhry,Kieran T Mellody,Amanda McGovern,Seiichiro Takahashi,C Adrian Shuttleworth,Cay M Kielty

The Journal of endocrinology 196:509-17 PubMed18310446

2008

Thyroid hormone stimulation of extracellular signal-regulated kinase and cell proliferation in human osteoblast-like cells is initiated at integrin alphaVbeta3.

Applications

Unspecified application

Species

Unspecified reactive species

A Scarlett,M P Parsons,P L Hanson,K K Sidhu,T P Milligan,J M Burrin

Human reproduction (Oxford, England) 22:3031-7 PubMed17890724

2007

Mifepristone, but not levonorgestrel, inhibits human blastocyst attachment to an in vitro endometrial three-dimensional cell culture model.

Applications

Unspecified application

Species

Unspecified reactive species

P G L Lalitkumar,S Lalitkumar,C X Meng,A Stavreus-Evers,F Hambiliki,U Bentin-Ley,Kristina Gemzell-Danielsson

The Journal of experimental medicine 203:1067-80 PubMed16606672

2006

Regulation of osteoclast function and bone mass by RAGE.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng Zhou,David Immel,Cai-Xia Xi,Angelika Bierhaus,Xu Feng,Lin Mei,Peter Nawroth,David M Stern,Wen-Cheng Xiong

Journal of cell science 108 ( Pt 3):1227-38 PubMed7542669

1995

Alpha v beta 1 is a receptor for vitronectin and fibrinogen, and acts with alpha 5 beta 1 to mediate spreading on fibronectin.

Applications

Unspecified application

Species

Unspecified reactive species

J F Marshall,D C Rutherford,A C McCartney,F Mitjans,S L Goodman,I R Hart

Cancer research 45:5663-9 PubMed4053038

1985

Monoclonal antibodies to osteoclastomas (giant cell bone tumors): definition of osteoclast-specific cellular antigens.

Applications

Unspecified application

Species

Unspecified reactive species

M A Horton,D Lewis,K McNulty,J A Pringle,T J Chambers
View all publications
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