AB78289

Anti-Integrin alpha V beta 3 antibody [27.1 (VNR-1)]

Name: Anti-Integrin alpha V beta 3 antibody [27.1 (VNR-1)] (ab78289) | Abcam
Brand: Abcam Limited
SKU: AB78289
Rating: 5 (5 reviews)

(5 Reviews)

(33 Publications)

Mouse Monoclonal Integrin alpha V antibody. Suitable for Flow Cyt and reacts with Human samples. Cited in 33 publications.

View Alternative Names

CD51, MSK8, VNRA, VTNR, ITGAV, Integrin alpha-V, Vitronectin receptor, Vitronectin receptor subunit alpha

1 Images

Flow Cytometry - Anti-Integrin alpha V beta 3 antibody [27.1 (VNR-1)] (AB78289)

Flow Cyt

Unknown

Flow Cytometry - Anti-Integrin alpha V beta 3 antibody [27.1 (VNR-1)] (AB78289)

Overlay histogram showing A431 cells stained with ab78289 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab78289, 2μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight^® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.

This image was generated using the ascites version of the product.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

27.1 (VNR-1)

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }

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Product details

This product was changed from ascites to tissue culture supernatant on 17 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Purification notes

Purified from TCS.

Storage buffer

pH: 7.6 Preservative: 0.1% Sodium azide Constituents: PBS, 1.45% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Integrin alpha V beta 3 also known as integrin αvβ3 or avb3 integrin is a transmembrane receptor with a molecular weight of about 140 kDa. This protein is composed of the alpha V (αv) and beta 3 (β3) subunits. Integrin αvβ3 is widely expressed in various cell types including endothelial cells osteoclasts and some tumor cells. Its function involves mechanical interaction with the extracellular matrix (ECM) allowing cells to adhere to ECM proteins such as vitronectin and fibronectin. This integrin plays an important role in cell adhesion migration and survival.

Biological function summary

Integrin alpha V beta 3 engages in various cellular processes by serving as a part of integrin complexes. It mediates signal transduction from the ECM to the cell interior influencing cellular responses such as shape changes survival and proliferation. The receptor regulates angiogenesis which is the formation of new blood vessels and osteoclast-mediated bone resorption. These functions are essential for tissue remodeling and repair. The integrin can also modulate immune responses by interacting with immune cells affecting inflammation and wound healing processes.

Pathways

Integrin alpha V beta 3 is an important component in signaling pathways such as the PI3K/AKT and MAPK pathways. These pathways facilitate the integrin's role in cell migration growth and survival. It interacts with proteins like focal adhesion kinase (FAK) and SRC kinase bridging signals from the ECM to intracellular signaling cascades. The integrin's ability to bind ligands and initiate these pathways makes it an important player in cellular responses to the extracellular environment influencing how cells align mechanically and biochemically.

Integrin alpha V beta 3 has significant associations with cancer progression and osteoporosis. In various cancers including melanoma and breast cancer overexpression of this integrin supports tumor angiogenesis and metastasis connecting its role with angiogenic growth factors and matrix metalloproteinases. In osteoporosis it collaborates with proteins like osteopontin promoting osteoclast attachment to the bone matrix thereby influencing bone degradation. Understanding its role in these disorders highlights potential therapeutic targets for inhibiting unwanted angiogenesis and excessive bone resorption.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The alpha-V (ITGAV) integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. ITGAV : ITGB3 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling (PubMed : 23125415). ITGAV : ITGB3 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed : 20682778). ITGAV : ITGB3 binds to FGF1 and this binding is essential for FGF1 signaling (PubMed : 18441324). ITGAV : ITGB3 binds to FGF2 and this binding is essential for FGF2 signaling (PubMed : 28302677). ITGAV : ITGB3 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed : 19578119). ITGAV : ITGB3 binds to IGF2 and this binding is essential for IGF2 signaling (PubMed : 28873464). ITGAV : ITGB3 binds to IL1B and this binding is essential for IL1B signaling (PubMed : 29030430). ITGAV : ITGB3 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed : 18635536, PubMed : 25398877). ITGAV : ITGB3 and ITGAV : ITGB6 act as receptors for fibrillin-1 (FBN1) and mediate R-G-D-dependent cell adhesion to FBN1 (PubMed : 12807887, PubMed : 17158881). Integrin alpha-V/beta-6 or alpha-V/beta-8 (ITGAV : ITGB6 or ITGAV : ITGB8) mediates R-G-D-dependent release of transforming growth factor beta-1 (TGF-beta-1) from regulatory Latency-associated peptide (LAP), thereby playing a key role in TGF-beta-1 activation (PubMed : 15184403, PubMed : 22278742, PubMed : 28117447). ITGAV : ITGB3 acts as a receptor for CD40LG (PubMed : 31331973). ITGAV : ITGB3 acts as a receptor for IBSP and promotes cell adhesion and migration to IBSP (PubMed : 10640428).. (Microbial infection) Integrin ITGAV : ITGB5 acts as a receptor for Adenovirus type C.. (Microbial infection) Integrin ITGAV : ITGB5 and ITGAV : ITGB3 act as receptors for Coxsackievirus A9 and B1.. (Microbial infection) Integrin ITGAV : ITGB3 acts as a receptor for Herpes virus 8/HHV-8.. (Microbial infection) Integrin ITGAV : ITGB6 acts as a receptor for herpes simplex 1/HHV-1.. (Microbial infection) Integrin ITGAV : ITGB3 acts as a receptor for Human parechovirus 1.. (Microbial infection) Integrin ITGAV : ITGB3 acts as a receptor for West nile virus.. (Microbial infection) In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.

See full target information ITGAV

Additional targets

ITGB3

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Publications (33)

Recent publications for all applications. Explore the full list and refine your search

Cell communication and signaling : CCS 23:139 PubMed40098186

2025

Inclusion of ΑVβ3 integrin into extracellular vesicles in a caveolin-1 tyrosine-14- phosphorylation dependent manner and subsequent transfer to recipient melanoma cells promotes migration, invasion and metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

R Huilcaman,A Campos,P Contreras,L Simón,M Varas-Godoy,F Grünenwald,Baohai Shao,Jay Heinecke,L Lobos-Gonzalez,L Leyton,A F G Quest

NMR in biomedicine :e4948 PubMed37038086

2023

Imaging angiogenesis in an intracerebrally induced model of brain macrometastasis using α β -targeted iron oxide microparticles.

Applications

Unspecified application

Species

Unspecified reactive species

Jessica Buck,Francisco Perez-Balderas,Niloufar Zarghami,Vanessa Johanssen,Alexandre A Khrapitchev,James R Larkin,Nicola R Sibson

Cell adhesion & migration 17:1-14 PubMed36944577

2023

Inhibition of Integrin αβ-FAK-MAPK signaling constrains the invasion of T-ALL cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lan Huang,Yao Zhu,Qinglin Kong,Xianmin Guan,Xiaoying Lei,Luying Zhang,Hui Yang,Xinyuan Yao,Shaoyan Liang,Xizhou An,Jie Yu

Biomolecules 12: PubMed36009051

2022

Periostin Augments Vascular Smooth Muscle Cell Calcification via β-Catenin Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Ioana Alesutan,Laura A Henze,Beate Boehme,Trang T D Luong,Daniel Zickler,Burkert Pieske,Kai-Uwe Eckardt,Andreas Pasch,Jakob Voelkl

Biomolecules 12: PubMed35204655

2022

Macrophage-like THP-1 Cells Derived from High-Density Cell Culture Are Resistant to TRAIL-Induced Cell Death via Down-Regulation of Death-Receptors DR4 and DR5.

Applications

Unspecified application

Species

Unspecified reactive species

Yana Vladimirovna Lomovskaya,Margarita Igorevna Kobyakova,Anatoly Sergeevich Senotov,Alexey Igorevich Lomovsky,Vladislav Valentinovich Minaychev,Irina Sergeevna Fadeeva,Daria Yuryevna Shtatnova,Kirill Sergeevich Krasnov,Alena Igorevna Zvyagina,Vladimir Semenovich Akatov,Roman Sergeevich Fadeev

Theranostics 11:1991-2005 PubMed33408794

2021

Remodeling cancer stemness by collagen/fibronectin the AKT and CDC42 signaling pathway crosstalk in glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Chuanhong Zhong,Bei Tao,Fangli Tang,Xiaobo Yang,Tangming Peng,Jian You,Kaiguo Xia,Xiangguo Xia,Ligang Chen,Lilei Peng

Oncotarget 11:4770-4787 PubMed33473260

2020

Alphastatin-C a new inhibitor of endothelial cell activation is a pro-arteriogenic agent and retards B16-F10 melanoma growth in a preclinical model.

Applications

Unspecified application

Species

Unspecified reactive species

Adilson Kleber Ferreira,Brunella Cristofaro,Milene Cristina Menezes,Ana Karina de Oliveira,Alexandre Keiji Tashima,Robson Lopes de Melo,Cristiane Castilho Fernandes Silva,Miryam Guillermina Palomino Rodriguez,Daniela Cajado de Oliveira Souza Carvalho,Ricardo Alexandre de Azevedo,Paulo Luiz de Sá Junior,Lisley Inata Mambelli,Fernanda Vieira Portaro,Luc Pardanaud,Anne Eichmann,Osvaldo Augusto Sant'Anna,Mxarcella Faria

eLife 9: PubMed32780016

2020

Irisin directly stimulates osteoclastogenesis and bone resorption in vitro and in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Eben G Estell,Phuong T Le,Yosta Vegting,Hyeonwoo Kim,Christiane Wrann,Mary L Bouxsein,Kenichi Nagano,Roland Baron,Bruce M Spiegelman,Clifford J Rosen

Bioorganic & medicinal chemistry 27:115094 PubMed31540828

2019

Preclinical evaluation of a dual sstr2 and integrin αβ-targeted heterodimer [Ga]-NOTA-3PEG-TATE-RGD.

Applications

Unspecified application

Species

Unspecified reactive species

Bingnan Liu,Zhenzhong Zhang,Hao Wang,Shaobo Yao

Graefe's archive for clinical and experimental oph 256:1589-1597 PubMed29931427

2018

Premacular membranes in tissue culture.

Applications

Unspecified application

Species

Unspecified reactive species

Denise Vogt,Franziska Vielmuth,Christian Wertheimer,Felix Hagenau,Stefanie R Guenther,Armin Wolf,Volker Spindler,Siegfried G Priglinger,Ricarda G Schumann

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Integrin alpha V beta 3 antibody [27.1 (VNR-1)]

Flow Cytometry - Anti-Integrin alpha V beta 3 antibody [27.1 (VNR-1)] (AB78289)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Reactivity data

Product details

Properties and storage information

Form

Purity

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (33)

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