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Anti-Integrin alpha V beta 3 antibody [LM609] (ab190147) is a mouse monoclonal antibody that is used to detect Integrin alpha V beta 3 in Flow Cytometry. Suitable for Human samples.



Images

Flow Cytometry - Anti-Integrin alpha V beta 3 antibody [LM609] (AB190147), expandable thumbnail
  • Flow Cytometry - Anti-Integrin alpha V beta 3 antibody [LM609] (AB190147), expandable thumbnail
  • Immunocytochemistry - Anti-Integrin alpha V beta 3 antibody [LM609] (AB190147), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICCFlow Cyt
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
5 µg/mL
Notes

-

Tested
Tested

Species
Human
Dilution info
0.10000-1.00000 µg for 106 Cells
Notes

-

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Target data

Function

Integrin alpha-V/beta-3 (ITGAV:ITGB3) is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIb/beta-3 (ITGA2B:ITGB3) is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain (By similarity). Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen (PubMed:9111081). This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. Fibrinogen binding enhances SELP expression in activated platelets (By similarity). ITGAV:ITGB3 binds to fractalkine (CX3CL1) and acts as its coreceptor in CX3CR1-dependent fractalkine signaling (PubMed:23125415, PubMed:24789099). ITGAV:ITGB3 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed:20682778). ITGAV:ITGB3 binds to FGF1 and this binding is essential for FGF1 signaling (PubMed:18441324). ITGAV:ITGB3 binds to FGF2 and this binding is essential for FGF2 signaling (PubMed:28302677). ITGAV:ITGB3 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed:19578119). ITGAV:ITGB3 binds to IGF2 and this binding is essential for IGF2 signaling (PubMed:28873464). ITGAV:ITGB3 binds to IL1B and this binding is essential for IL1B signaling (PubMed:29030430). ITGAV:ITGB3 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed:18635536, PubMed:25398877). ITGAV:ITGB3 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1 (PubMed:12807887). In brain, plays a role in synaptic transmission and plasticity. Involved in the regulation of the serotonin neurotransmission, is required to localize to specific compartments within the synapse the serotonin receptor SLC6A4 and for an appropriate reuptake of serotonin. Controls excitatory synaptic strength by regulating GRIA2-containing AMPAR endocytosis, which affects AMPAR abundance and composition (By similarity). ITGAV:ITGB3 act as a receptor for CD40LG (PubMed:31331973). ITGAV:ITGB3 acts as a receptor for IBSP and promotes cell adhesion and migration to IBSP (PubMed:10640428). (Microbial infection) Integrin ITGAV:ITGB3 acts as a receptor for Herpes virus 8/HHV-8. (Microbial infection) Integrin ITGAV:ITGB3 acts as a receptor for Coxsackievirus A9. (Microbial infection) Acts as a receptor for Hantaan virus. (Microbial infection) Integrin ITGAV:ITGB3 acts as a receptor for Cytomegalovirus/HHV-5. (Microbial infection) Integrin ITGA5:ITGB3 acts as a receptor for Human metapneumovirus. (Microbial infection) Integrin ITGAV:ITGB3 acts aP05556s a receptor for Human parechovirus 1. (Microbial infection) Integrin ITGAV:ITGB3 acts as a receptor for West nile virus. (Microbial infection) In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.

Alternative names

CD61, GP3A, ITGB3, Integrin beta-3, Platelet membrane glycoprotein IIIa, GPIIIa

Recommended products

Anti-Integrin alpha V beta 3 antibody [LM609] (ab190147) is a mouse monoclonal antibody that is used to detect Integrin alpha V beta 3 in Flow Cytometry. Suitable for Human samples.


Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
LM609
Purification technique
Affinity purification Protein G
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C

Notes

What is this antibody validated in?


Anti-Integrin alpha V beta 3 antibody [LM609] (ab190147) is a mouse monoclonal antibody and is validated for use in Flow Cytometry (Flow Cyt), in Human samples.

Recommended positive controls


ICC: Malme-3M cells.Flow Cytometry: HUVEC cells.

Reviewed by scientists


Anti-Integrin alpha V beta 3 [LM609] (ab190147) has over 5 independent reviews from customers.

Other related products


We have a range of other formats of antibody clone [LM609] also available for your convenience:
ab190147, Carrier free - Anti-Integrin alpha V beta 3 antibody [LM609] - BSA and Azide free ab238667



Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Integrin alpha V beta 3 also known as integrin αvβ3 or avb3 integrin is a transmembrane receptor with a molecular weight of about 140 kDa. This protein is composed of the alpha V (αv) and beta 3 (β3) subunits. Integrin αvβ3 is widely expressed in various cell types including endothelial cells osteoclasts and some tumor cells. Its function involves mechanical interaction with the extracellular matrix (ECM) allowing cells to adhere to ECM proteins such as vitronectin and fibronectin. This integrin plays an important role in cell adhesion migration and survival.

Biological function summary

Integrin alpha V beta 3 engages in various cellular processes by serving as a part of integrin complexes. It mediates signal transduction from the ECM to the cell interior influencing cellular responses such as shape changes survival and proliferation. The receptor regulates angiogenesis which is the formation of new blood vessels and osteoclast-mediated bone resorption. These functions are essential for tissue remodeling and repair. The integrin can also modulate immune responses by interacting with immune cells affecting inflammation and wound healing processes.

Pathways

Integrin alpha V beta 3 is an important component in signaling pathways such as the PI3K/AKT and MAPK pathways. These pathways facilitate the integrin's role in cell migration growth and survival. It interacts with proteins like focal adhesion kinase (FAK) and SRC kinase bridging signals from the ECM to intracellular signaling cascades. The integrin's ability to bind ligands and initiate these pathways makes it an important player in cellular responses to the extracellular environment influencing how cells align mechanically and biochemically.

Associated diseases and disorders

Integrin alpha V beta 3 has significant associations with cancer progression and osteoporosis. In various cancers including melanoma and breast cancer overexpression of this integrin supports tumor angiogenesis and metastasis connecting its role with angiogenic growth factors and matrix metalloproteinases. In osteoporosis it collaborates with proteins like osteopontin promoting osteoclast attachment to the bone matrix thereby influencing bone degradation. Understanding its role in these disorders highlights potential therapeutic targets for inhibiting unwanted angiogenesis and excessive bone resorption.

Product promise

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3 product images

  • Flow Cytometry - Anti-Integrin alpha V beta 3 antibody [LM609] (ab190147), expandable thumbnail

    Flow Cytometry - Anti-Integrin alpha V beta 3 antibody [LM609] (ab190147)

    Overlay histogram showing HUVEC cells stained with ab190147 (red line). The cells were incubated in 1x PBS containing 10 % normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab190147) (1 μg/1x106 cells) for 30 min on ice. The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor® 488, pre-adsorbed) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117) was used at 1/2000 for 30 min on ice.

    Isotype control antibody (black line) was mouse IgG1κ (Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control ab170190) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.

  • Flow Cytometry - Anti-Integrin alpha V beta 3 antibody [LM609] (ab190147), expandable thumbnail

    Flow Cytometry - Anti-Integrin alpha V beta 3 antibody [LM609] (ab190147)

    Overlay histogram showing HUVEC (Human umbilical vein endothelial cell line) cells stained with ab190147 (red line). The cells were fixed with 4% formaldehyde (10 minutes) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab190147, 1 μg/1x106 cells) for 30 minutes at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113) at 1/4000 dilution for 30 minutes at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (Anti-Dopamine beta Hydroxylase antibody ab19353, 1 μg/1x106 cells) used under the same conditions.

    Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

  • Immunocytochemistry - Anti-Integrin alpha V beta 3 antibody [LM609] (ab190147), expandable thumbnail

    Immunocytochemistry - Anti-Integrin alpha V beta 3 antibody [LM609] (ab190147)

    ab190147 staining Integrin Alpha V beta 3 in Malme-3M cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab190147 at 5µg/ml and Anti-beta Tubulin antibody - Loading Control ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.

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