AB190147

Anti-Integrin alpha V beta 3 antibody [LM609]

Name: Anti-Integrin alpha V beta 3 antibody [LM609] (ab190147) | Abcam
Brand: Abcam Limited
SKU: AB190147
Price: 510 USD
Availability: InStock
Rating: 4 (6 reviews)

(6 Reviews)

(17 Publications)

Anti-Integrin alpha V beta 3 antibody [LM609] (ab190147) is a mouse monoclonal antibody detecting Integrin alpha V beta 3 in Flow Cytometry. Suitable for Human.

View Alternative Names

CD61, GP3A, ITGB3, Integrin beta-3, Platelet membrane glycoprotein IIIa, GPIIIa

3 Images

Flow Cytometry - Anti-Integrin alpha V beta 3 antibody [LM609] (AB190147)

Flow Cyt

Lab

Flow Cytometry - Anti-Integrin alpha V beta 3 antibody [LM609] (AB190147)

Overlay histogram showing HUVEC (Human umbilical vein endothelial cell line) cells stained with ab190147 (red line). The cells were fixed with 4% formaldehyde (10 minutes) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab190147, 1 μg/1x10⁶ cells) for 30 minutes at 22°C. The secondary antibody used was Alexa Fluor^® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/4000 dilution for 30 minutes at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab19353, 1 μg/1x10⁶ cells) used under the same conditions.

Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Flow Cyt

Lab

Flow Cytometry - Anti-Integrin alpha V beta 3 antibody [LM609] (AB190147)

Overlay histogram showing HUVEC cells stained with ab190147 (red line). The cells were incubated in 1x PBS containing 10 % normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab190147) (1 μg/1x10⁶ cells) for 30 min on ice. The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor® 488, pre-adsorbed) (ab150117) was used at 1/2000 for 30 min on ice.

Isotype control antibody (black line) was mouse IgG1κ (ab170190) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.

Lab

Immunocytochemistry - Anti-Integrin alpha V beta 3 antibody [LM609] (AB190147)

ab190147 staining Integrin Alpha V beta 3 in Malme-3M cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab190147 at 5µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor^® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.

Carrier free

Anti-Integrin alpha V beta 3 antibody [LM609] - BSA and Azide free
578 PE

PE Anti-Integrin alpha V beta 3 antibody [LM609]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

LM609

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

Flow Cyt, ICC

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "5 µg/mL", "ICC-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.1-1 µg for 10^6 Cells", "FlowCyt-species-notes": "<p></p>" } } }

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Product details

What is this antibody validated in?
Anti-Integrin alpha V beta 3 antibody [LM609] (ab190147) is a mouse monoclonal antibody and is validated for use in Flow Cytometry (Flow Cyt) in Human samples.

Reviewed by scientists
Anti-Integrin alpha V beta 3 [LM609] (ab190147) has over 5 independent reviews from customers.

Other related products
We have a range of other formats of antibody clone [LM609] also available for your convenience: ab190147, Carrier free - ab238667

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 6.97% L-Arginine

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Integrin alpha V beta 3 also known as integrin αvΒ3 or avb3 integrin is a transmembrane receptor with a molecular weight of about 140 kDa. This protein is composed of the alpha V (αv) and beta 3 (Β3) subunits. Integrin αvΒ3 is widely expressed in various cell types including endothelial cells osteoclasts and some tumor cells. Its function involves mechanical interaction with the extracellular matrix (ECM) allowing cells to adhere to ECM proteins such as vitronectin and fibronectin. This integrin plays an important role in cell adhesion migration and survival.

Biological function summary

Integrin alpha V beta 3 engages in various cellular processes by serving as a part of integrin complexes. It mediates signal transduction from the ECM to the cell interior influencing cellular responses such as shape changes survival and proliferation. The receptor regulates angiogenesis which is the formation of new blood vessels and osteoclast-mediated bone resorption. These functions are essential for tissue remodeling and repair. The integrin can also modulate immune responses by interacting with immune cells affecting inflammation and wound healing processes.

Pathways

Integrin alpha V beta 3 is an important component in signaling pathways such as the PI3K/AKT and MAPK pathways. These pathways facilitate the integrin's role in cell migration growth and survival. It interacts with proteins like focal adhesion kinase (FAK) and SRC kinase bridging signals from the ECM to intracellular signaling cascades. The integrin's ability to bind ligands and initiate these pathways makes it an important player in cellular responses to the extracellular environment influencing how cells align mechanically and biochemically.

Integrin alpha V beta 3 has significant associations with cancer progression and osteoporosis. In various cancers including melanoma and breast cancer overexpression of this integrin supports tumor angiogenesis and metastasis connecting its role with angiogenic growth factors and matrix metalloproteinases. In osteoporosis it collaborates with proteins like osteopontin promoting osteoclast attachment to the bone matrix thereby influencing bone degradation. Understanding its role in these disorders highlights potential therapeutic targets for inhibiting unwanted angiogenesis and excessive bone resorption.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Integrin alpha-V/beta-3 (ITGAV : ITGB3) is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIb/beta-3 (ITGA2B : ITGB3) is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain (By similarity). Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen (PubMed : 9111081). This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. Fibrinogen binding enhances SELP expression in activated platelets (By similarity). ITGAV : ITGB3 binds to fractalkine (CX3CL1) and acts as its coreceptor in CX3CR1-dependent fractalkine signaling (PubMed : 23125415, PubMed : 24789099). ITGAV : ITGB3 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed : 20682778). ITGAV : ITGB3 binds to FGF1 and this binding is essential for FGF1 signaling (PubMed : 18441324). ITGAV : ITGB3 binds to FGF2 and this binding is essential for FGF2 signaling (PubMed : 28302677). ITGAV : ITGB3 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed : 19578119). ITGAV : ITGB3 binds to IGF2 and this binding is essential for IGF2 signaling (PubMed : 28873464). ITGAV : ITGB3 binds to IL1B and this binding is essential for IL1B signaling (PubMed : 29030430). ITGAV : ITGB3 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed : 18635536, PubMed : 25398877). ITGAV : ITGB3 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1 (PubMed : 12807887). In brain, plays a role in synaptic transmission and plasticity. Involved in the regulation of the serotonin neurotransmission, is required to localize to specific compartments within the synapse the serotonin receptor SLC6A4 and for an appropriate reuptake of serotonin. Controls excitatory synaptic strength by regulating GRIA2-containing AMPAR endocytosis, which affects AMPAR abundance and composition (By similarity). ITGAV : ITGB3 act as a receptor for CD40LG (PubMed : 31331973). ITGAV : ITGB3 acts as a receptor for IBSP and promotes cell adhesion and migration to IBSP (PubMed : 10640428). Integrin ITGA2B : ITGB3 is also the receptor of the erythrocyte-specific ICAM4 ligand involved in heterotypic cell-cell adhesion between erythrocytes and activated platelets (PubMed : 12477717).. (Microbial infection) Integrin ITGAV : ITGB3 acts as a receptor for Herpes virus 8/HHV-8.. (Microbial infection) Integrin ITGAV : ITGB3 acts as a receptor for Coxsackievirus A9.. (Microbial infection) Acts as a receptor for Hantaan virus.. (Microbial infection) Integrin ITGAV : ITGB3 acts as a receptor for Cytomegalovirus/HHV-5.. (Microbial infection) Integrin ITGA5 : ITGB3 acts as a receptor for Human metapneumovirus.. (Microbial infection) Integrin ITGAV : ITGB3 acts aP05556s a receptor for Human parechovirus 1.. (Microbial infection) Integrin ITGAV : ITGB3 acts as a receptor for West nile virus.. (Microbial infection) In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.

See full target information ITGB3

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Publications (17)

Recent publications for all applications. Explore the full list and refine your search

Proceedings of the National Academy of Sciences of the United States of America 122:e2423700122 PubMed40244661

2025

Intussusceptive angiogenesis-on-a-chip: Evidence for transluminal vascular bridging by endothelial delamination.

Applications

Unspecified application

Species

Unspecified reactive species

Sabrina C R Staples,Hao Yin,Frances S K Sutherland,Emma K Prescott,Dylan Tinney,Douglas W Hamilton,Daniel Goldman,Tamie L Poepping,Christopher G Ellis,J Geoffrey Pickering

Acta pharmaceutica Sinica. B 15:1143-1158 PubMed40177554

2025

Modulating active targeting nanoparticle design according to tumor progressions.

Applications

Unspecified application

Species

Unspecified reactive species

Huifang Nie,Rong Huang,Guangwei Jiang,Wenshuai Li,Lan Yang,Meng Zhang,Min Qian,Wei Guo,Tao Ye,Rongqin Huang

International journal of molecular sciences 26: PubMed39940950

2025

Effects of Cell Seeding Density, Extracellular Matrix Composition, and Geometry on Yes-Associated Protein Translocation in Corneal Fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Divya Subramanian,Nathaniel S Tjahjono,Satweka Nammi,Miguel Miron-Mendoza,Victor D Varner,W Matthew Petroll,David W Schmidtke

Breast cancer research : BCR 26:187 PubMed39707454

2024

Cilengitide sensitivity is predicted by overall integrin expression in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Nomeda Girnius,Aylin Z Henstridge,Benjamin Marks,Jeffrey K Yu,G Kenneth Gray,Chris Sander,Ioannis K Zervantonakis,Augustin Luna

International journal of molecular sciences 24: PubMed37894778

2023

Dupuytren's Disease Is Mediated by Insufficient TGF-β1 Release and Degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Lisa Oezel,Marie Wohltmann,Nele Gondorf,Julia Wille,Irmak Güven,Joachim Windolf,Simon Thelen,Carina Jaekel,Vera Grotheer

Science advances 9:eade2120 PubMed37585527

2023

Fibroblasts generate topographical cues that steer cancer cell migration.

Applications

Unspecified application

Species

Unspecified reactive species

Francesco Baschieri,Abigail Illand,Jorge Barbazan,Olivier Zajac,Clémence Henon,Damarys Loew,Florent Dingli,Danijela Matic Vignjevic,Sandrine Lévêque-Fort,Guillaume Montagnac

Lab on a chip 23:1674-1693 PubMed36779251

2023

Tumour associated vasculature-on-a-chip for the evaluation of microbubble-mediated delivery of targeted liposomes.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew D Bourn,Safoura Zahed Mohajerani,Georgia Mavria,Nicola Ingram,P Louise Coletta,Stephen D Evans,Sally A Peyman

BMC biotechnology 22:15 PubMed35597930

2022

αvβ3-targeted sEVs for efficient intracellular delivery of proteins using MFG-E8.

Applications

Unspecified application

Species

Unspecified reactive species

Junxin Mai,Kai Wang,Chenxuexuan Liu,Sheng Xiong,Qiuling Xie

Molecular medicine reports 25: PubMed35543146

2022

Curcumin improves the function of umbilical vein endothelial cells by inhibiting HO‑induced pyroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yulin Yuan,Caiping Zhang,Yunwu He,Lingzhi Yuan,Qian Zhao,Yuhe Liu,Shiyin Long

BMC cancer 22:459 PubMed35473511

2022

Deletion of TRIB3 disrupts the tumor progression induced by integrin αvβ3 in lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Wen Zhou,Junjun Ma,Lifeng Meng,Dabei Liu,Jun Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Integrin alpha V beta 3 antibody [LM609]

Flow Cytometry - Anti-Integrin alpha V beta 3 antibody [LM609] (AB190147)

Flow Cytometry - Anti-Integrin alpha V beta 3 antibody [LM609] (AB190147)

Immunocytochemistry - Anti-Integrin alpha V beta 3 antibody [LM609] (AB190147)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (17)

Product promise