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AB223605

Anti-Integrin alpha V+beta 5 antibody [P1F6] - BSA and Azide free

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(1 Publication)

Mouse Monoclonal Integrin alpha V antibody. Carrier free. Suitable for Flow Cyt, ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

CD51, MSK8, VNRA, VTNR, ITGAV, Integrin alpha-V, Vitronectin receptor, Vitronectin receptor subunit alpha, Integrin beta-5, ITGB5

2 Images
Flow Cytometry - Anti-Integrin alpha V+beta 5 antibody [P1F6] - BSA and Azide free (AB223605)
  • Flow Cyt

Lab

Flow Cytometry - Anti-Integrin alpha V+beta 5 antibody [P1F6] - BSA and Azide free (AB223605)

This data was developed using the same antibody clone in a different buffer formulation (ab223605)

Flow Cytometry Overlay histogram showing A549 cells stained with ab223605 (red line). The cells were incubated in 1x PBS containing 10 % normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab223605) (1x106 cells in 100μl at 1μg/ml) for 30 min on ice.

The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor® 488, pre-adsorbed) (ab150117) was used at 1/2000 for 30 min on ice.

Isotype control antibody (black line) was mouse IgG1κ; (ab170190) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.

Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha V+beta 5 antibody [P1F6] - BSA and Azide free (AB223605)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha V+beta 5 antibody [P1F6] - BSA and Azide free (AB223605)

This data was developed using the same antibody clone in a different buffer formulation containing BSA and sodium azide (ab177004).

ab177004 staining Integrin alpha V+beta 5 in A549 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab177004 at 1µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue).

Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

P1F6

Isotype

IgG1

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Assay Kits

AB235650

Human CD51 ELISA Kit

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Sandwich ELISA - Human CD51 ELISA Kit (AB235650)

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Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

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Primary Antibodies

AB30394

Anti-Integrin beta 1 antibody [12G10]

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Immunocytochemistry/ Immunofluorescence - Anti-Integrin beta 1 antibody [12G10] (AB30394)

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Primary Antibodies

AB150361

Anti-Integrin alpha 5 antibody [EPR7854]

RabMAb|Recombinant|KO Validated|20ul selling size

Western blot - Anti-Integrin alpha 5 antibody [EPR7854] (AB150361)

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Primary Antibodies

AB184312

Anti-Integrin beta 5 antibody [EPR19170]

RabMAb|Recombinant

Western blot - Anti-Integrin beta 5 antibody [EPR19170] (AB184312)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.1-1 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Integrin alpha V beta 5 also referred to as αVβ5 or integrin alpha V is a heterodimeric protein composed of alpha V and beta 5 subunits. It has a molecular mass of approximately 210 kDa. This integrin is found on the cell surface primarily in epithelial cells such as those within the airway. The structure enables it to bind to the extracellular matrix proteins facilitating cell adhesion and signaling.
Biological function summary

Integrin alpha V beta 5 plays an essential role in angiogenesis and tissue repair. It acts as a receptor for vitronectin and fibronectin mediating cell-matrix interactions. This integrin is part of a larger integrin family which forms complexes to execute cellular adhesion and migration processes. Its ability to bind to multiple ligands highlights its flexibility in function.

Pathways

Integrin alpha V beta 5 is deeply engaged in the FAK (focal adhesion kinase) and MAPK (mitogen-activated protein kinase) pathways. These pathways are significant in cell survival proliferation and differentiation. Integrin alpha V beta 5 interacts with other proteins such as integrin beta 3 to regulate cellular responses to environmental cues linking the extracellular matrix to intracellular signaling pathways.

Integrin alpha V beta 5 has been linked to cancer progression and fibrosis. Its expression correlates with tumor invasiveness and metastatic potential by promoting angiogenesis and tissue invasion. In fibrosis it contributes to the excessive buildup of extracellular matrix. The interaction with proteins like TGF-β (transforming growth factor-beta) is notable in these disease processes influencing the pathophysiology by modulating cellular responses to growth factors.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The alpha-V (ITGAV) integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. ITGAV : ITGB3 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling (PubMed : 23125415). ITGAV : ITGB3 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed : 20682778). ITGAV : ITGB3 binds to FGF1 and this binding is essential for FGF1 signaling (PubMed : 18441324). ITGAV : ITGB3 binds to FGF2 and this binding is essential for FGF2 signaling (PubMed : 28302677). ITGAV : ITGB3 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed : 19578119). ITGAV : ITGB3 binds to IGF2 and this binding is essential for IGF2 signaling (PubMed : 28873464). ITGAV : ITGB3 binds to IL1B and this binding is essential for IL1B signaling (PubMed : 29030430). ITGAV : ITGB3 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed : 18635536, PubMed : 25398877). ITGAV : ITGB3 and ITGAV : ITGB6 act as receptors for fibrillin-1 (FBN1) and mediate R-G-D-dependent cell adhesion to FBN1 (PubMed : 12807887, PubMed : 17158881). Integrin alpha-V/beta-6 or alpha-V/beta-8 (ITGAV : ITGB6 or ITGAV : ITGB8) mediates R-G-D-dependent release of transforming growth factor beta-1 (TGF-beta-1) from regulatory Latency-associated peptide (LAP), thereby playing a key role in TGF-beta-1 activation (PubMed : 15184403, PubMed : 22278742, PubMed : 28117447). ITGAV : ITGB3 acts as a receptor for CD40LG (PubMed : 31331973). ITGAV : ITGB3 acts as a receptor for IBSP and promotes cell adhesion and migration to IBSP (PubMed : 10640428).. (Microbial infection) Integrin ITGAV : ITGB5 acts as a receptor for Adenovirus type C.. (Microbial infection) Integrin ITGAV : ITGB5 and ITGAV : ITGB3 act as receptors for Coxsackievirus A9 and B1.. (Microbial infection) Integrin ITGAV : ITGB3 acts as a receptor for Herpes virus 8/HHV-8.. (Microbial infection) Integrin ITGAV : ITGB6 acts as a receptor for herpes simplex 1/HHV-1.. (Microbial infection) Integrin ITGAV : ITGB3 acts as a receptor for Human parechovirus 1.. (Microbial infection) Integrin ITGAV : ITGB3 acts as a receptor for West nile virus.. (Microbial infection) In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
See full target information ITGAV

Additional targets

Integrin beta-5
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Signal transduction and targeted therapy 10:223 PubMed40670349

2025

Comprehensive genomic and transcriptomic analyses reveal prognostic stratification for esophageal squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Gao,Qiming Wang,Fangqiu Fu,Yue Zhao,Teng Yang,Xiangze Li,Yihua Sun,Hong Hu,Longfei Ma,Longsheng Miao,Xiaoyang Luo,Ting Ye,Yiliang Zhang,Yang Zhang,Ziling Huang,Hang Li,Longlong Shao,Midie Xu,Kuaile Zhao,Shiyue Zhang,Mou Zhang,Jun Wang,Chong Dai,Xiaoxiao Shang,Tingyi An,Yawei Zhang,Jiaqing Xiang,Zhiwei Cao,Bin Li,Haiquan Chen
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Product promise

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