AB183666

Anti-Integrin beta 1 antibody

Name: Anti-Integrin beta 1 antibody. Rabbit polyclonal (ab183666) | Abcam
Brand: Abcam Limited
SKU: AB183666
Price: 475 USD
Availability: InStock
Rating: 3 (3 reviews)

(3 Reviews)

(43 Publications)

Rabbit Polyclonal Integrin beta 1 antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat, Dog samples. Cited in 43 publications. Immunogen corresponding to Synthetic Peptide within Human ITGB1 aa 700 to C-terminus.

View Alternative Names

CD29, FNRB, MDF2, MSK12, ITGB1, Integrin beta-1, Fibronectin receptor subunit beta, Glycoprotein IIa, VLA-4 subunit beta, GPIIA

22 Images

Immunocytochemistry/ Immunofluorescence - Anti-Integrin beta 1 antibody (AB183666)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Integrin beta 1 antibody (AB183666)

Immunofluorescence analysis of ice-cold MeOH fixed A431 cells labelling Integrin beta 1 / CD29 protein at cell membrane using ab183666 at a 1/500 dilution for 5 min (Green). The nuclear counter stain is DAPI (Blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 1 antibody (AB183666)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 1 antibody (AB183666)

Paraffin-embedded human colon cancer tissue stained for Integrin beta 1 / CD29 protein at cell membrane and cytoplasm with ab183666 at a 1/500 dilution in immunohistochemical analysis.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 1 antibody (AB183666)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling Integrin beta 1 with ab183666 at 1/500 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 1 antibody (AB183666)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Integrin beta 1 with ab322405 at 1/2000 (0.249 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human colon in (A) ab322405 and (B) ab183666.
The image on (A) ab322405 and (B) ab183666 are treated with Anti-ITGB1 antibody at 1/2000 dilution. The section was incubated with ab322405 or ab183666 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Integrin beta 1 antibody (AB183666)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 1 antibody (AB183666)

Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Integrin beta 1 with ab322405 at 1/2000 (0.249 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human hepatocellular carcinoma in (A) ab322405 and (B) ab183666. The image on (A) ab322405 and (B) ab183666 are treated with Anti-ITGB1 antibody at 1/2000 dilution. The section was incubated with ab322405 or ab183666 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Supplier Data

Immunoprecipitation - Anti-Integrin beta 1 antibody (AB183666)

ab183666 at 5μg immunoprecipitating Integrin beta 1 in HeLa whole cell extracts.

All lanes:

Immunoprecipitation - Anti-Integrin beta 1 antibody (ab183666)

Predicted band size: 88 kDa

false

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 1 antibody (AB183666)

Paraffin-embedded mouse tissue stained for Integrin beta 1 / CD29 protein with ab183666 at a 1/500 dilution in immunohistochemical analysis.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 1 antibody (AB183666)

Paraffin-embedded mouse brown adipocyte tissue stained for Integrin beta 1 / CD29 protein at cytoplasm with ab183666 at a 1/500 dilution in immunohistochemical analysis.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

Supplier Data

Western blot - Anti-Integrin beta 1 antibody (AB183666)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 34646995; PMID : 19940135 ).

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

In Western blot, ab322405 was shown to bind specifically to ITGB1. A band was observed at 110-140 kDa in wild-type HeLa cell lysates with whereas no signal observed at this size in ITGB1 knockout cell line.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Left panel : Lanes 1-6 : 81 seconds; Lanes 7-8 : 37 seconds Right panel : 26 seconds

Observed MW(kDa) 110-140.

All lanes:

Western blot - Anti-Integrin beta 1 antibody [RM1259] (<a href='/en-us/products/primary-antibodies/integrin-beta-1-antibody-rm1259-ab322405'>ab322405</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

ITGB1 knockout HeLa whole cell lysate at 20 µg

Lane 3:

U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 5:

HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 6:

IMR-32 (human neuroblastoma neuroblast) whole cell lysate at 20 µg

Lane 7:

Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

Lane 8:

C2C12 (mouse myoblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 110-140 kDa,36 kDa

false

Supplier Data

Western blot - Anti-Integrin beta 1 antibody (AB183666)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-Integrin beta 1 antibody (ab183666) at 1/10000 dilution

Lane 1:

293T whole cell extracts at 30 µg

Lane 2:

A431 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 88 kDa

false

Supplier Data

Western blot - Anti-Integrin beta 1 antibody (AB183666)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-Integrin beta 1 antibody (ab183666) at 1/10000 dilution

Lane 1:

U87-MG whole cell extracts at 30 µg

Lane 2:

SK-N-SH whole cell extracts at 30 µg

Lane 3:

IMR32 whole cell extracts at 30 µg

Lane 4:

SK-N-AS whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 88 kDa

false

Supplier Data

Western blot - Anti-Integrin beta 1 antibody (AB183666)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-Integrin beta 1 antibody (ab183666) at 1/10000 dilution

Lane 1:

IMR32 whole cell extract at 30 µg

Lane 2:

SK-N-AS whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 88 kDa

false

Supplier Data

Western blot - Anti-Integrin beta 1 antibody (AB183666)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-Integrin beta 1 antibody (ab183666) at 1/400 dilution

Lane 1:

Wild-type (WT) HeLa cell extracts at 30 µg

Lane 2:

Integrin beta 1 / CD29 knockout (KO) HeLa cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 88 kDa

false

Supplier Data

Western blot - Anti-Integrin beta 1 antibody (AB183666)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Observed MW(kDa) 110-140.

All lanes:

Western blot - Anti-Integrin beta 1 antibody [RM1259] (<a href='/en-us/products/primary-antibodies/integrin-beta-1-antibody-rm1259-ab322405'>ab322405</a>) at 1/1000 dilution

Lane 1:

Human liver tissue lysate at 20 µg

Lane 2:

Mouse heart tissue lysate at 20 µg

Lane 3:

Mouse kidney tissue lysate at 20 µg

Lane 4:

Mouse spleen tissue lysate at 20 µg

Lane 5:

Mouse liver tissue lysate at 20 µg

Lane 6:

Rat heart tissue lysate at 20 µg

Lane 7:

Rat kidney tissue lysate at 20 µg

Lane 8:

Rat spleen tissue lysate at 20 µg

Lane 9:

Rat liver tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 110-140 kDa,36 kDa

false

Exposure time: 81s

Supplier Data

Western blot - Anti-Integrin beta 1 antibody (AB183666)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Observed MW(kDa) 110-140.

All lanes:

Western blot - Anti-Integrin beta 1 antibody [RM1259] (<a href='/en-us/products/primary-antibodies/integrin-beta-1-antibody-rm1259-ab322405'>ab322405</a>) at 1/1000 dilution

Lane 1:

Human cerebellum tissue lysate at 20 µg

Lane 2:

Human skeletal muscle tissue lysate at 20 µg

Lane 3:

Human spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 110-140 kDa

false

Exposure time: 26s

Supplier Data

Western blot - Anti-Integrin beta 1 antibody (AB183666)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-Integrin beta 1 antibody (ab183666) at 1/10000 dilution

All lanes:

Mouse brain tissue at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 88 kDa

false

Supplier Data

Western blot - Anti-Integrin beta 1 antibody (AB183666)

Samples were separated by 7.5% SDS-PAGE. The differentiation stimulated medium is composed by basal medium, 10% FBS, 50 ug/ml gentamicin, 1 nM L-glutamin, 500 uM IBMX, 1 uM dexamethasone, 2 uM rosiglitazone and 1 ug/ml insulin.

All lanes:

Western blot - Anti-Integrin beta 1 antibody (ab183666) at 1/500 dilution

Lane 1:

Unstimulatd 3T3-L1 whole cell extracts at 20 µg

Lane 2:

Stimulatd 3T3-L1 whole cell extracts at 20 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 88 kDa

false

Supplier Data

Western blot - Anti-Integrin beta 1 antibody (AB183666)

7.5 % SDS-PAGE

All lanes:

Western blot - Anti-Integrin beta 1 antibody (ab183666) at 1/5000 dilution

Lane 1:

PC-12 whole cell lysate/extract at 30 µg

Lane 2:

Rat-2 whole cell lysate/extract at 30 µg

Predicted band size: 88 kDa

false

Supplier Data

Western blot - Anti-Integrin beta 1 antibody (AB183666)

7.5 % SDS-PAGE

All lanes:

Western blot - Anti-Integrin beta 1 antibody (ab183666) at 1/5000 dilution

Lane 1:

NIH-3T3 whole cell lysate/extract at 30 µg

Lane 2:

BCL-1 whole cell lysate/extract at 30 µg

Lane 3:

Raw264.7 whole cell lysate/extract at 30 µg

Lane 4:

C2C12 whole cell lysate/extract at 30 µg

Predicted band size: 88 kDa

false

Supplier Data

Western blot - Anti-Integrin beta 1 antibody (AB183666)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Exposure times : Left panel : 26 seconds; right panel : 15 seconds.

Observed molecular weight 110-140 kDa.

All lanes:

Western blot - Anti-Integrin beta 1 antibody [RM1259] (<a href='/en-us/products/primary-antibodies/integrin-beta-1-antibody-rm1259-ab322405'>ab322405</a>) at 1/1000 dilution

Lane 1:

C6 (rat glial tumor glial cell) whole cell lysate at 20 µg

Lane 2:

RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

Lane 3:

PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg

Lane 4:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 110-140 kDa

false

Supplier Data

Western blot - Anti-Integrin beta 1 antibody (AB183666)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-Integrin beta 1 antibody (ab183666) at 1/5000 dilution

All lanes:

MDCK Whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 88 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human, Dog

Applications

IHC-P, ICC/IF, IP, WB

applications

Immunogen

Synthetic Peptide within Human ITGB1 aa 700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P05556

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Integrin beta 1 also known as CD29 ITGB1 or integrin β1 is a part of the integrin family of cell surface receptors. This protein plays a role in cell adhesion and signal transduction processes. Integrin beta 1 weighs around 88 kDa and typically expresses on the surface of various cell types including epithelial endothelial and certain blood cells. As a transmembrane receptor it interacts with extracellular matrix components such as fibronectin collagen and laminin facilitating cell-matrix adhesion which is key in maintaining cellular structure and function.

Biological function summary

Integrin beta 1 functions in cells as a component of heterodimeric complexes with alpha integrin subunits forming adhesion receptors that mediate cellular signaling. These integrins are important for processes like cell migration differentiation proliferation and apoptosis. As they interact with various extracellular ligands integrin beta 1 influences numerous cellular responses particularly in tissue remodeling wound healing and embryonic development.

Pathways

Integrin beta 1 plays significant roles in the MAPK/ERK and PI3K/Akt signaling pathways both of which are essential for cellular growth and survival signals. It forms connections with associated proteins including focal adhesion kinase (FAK) and talin which participate in mechanotransduction and signal propagation. These interactions influence cellular responses to environmental changes impacting processes such as tissue repair and immune response modulation.

Integrin beta 1 associates with cancer progression and fibrosis disorders. Abnormal expression or function of integrin beta 1 can promote tumor invasion and metastasis making it a target for cancer research and potential therapeutic interventions. Furthermore in fibrosis integrin beta 1 interacts with transforming growth factor-beta (TGF-β) signaling contributing to excessive extracellular matrix production and scarring. Understanding these relationships helps in developing targeted treatments for conditions where integrin beta 1 plays a critical role.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta-1 and alpha-11/beta-1 are receptors for collagen. Integrins alpha-1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Integrins alpha-2/beta-1, alpha-3/beta-1, alpha-4/beta-1, alpha-5/beta-1, alpha-8/beta-1, alpha-10/beta-1, alpha-11/beta-1 and alpha-V/beta-1 are receptors for fibronectin. Alpha-4/beta-1 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. Integrin alpha-5/beta-1 is a receptor for fibrinogen. Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin. Integrin alpha-6/beta-1 (ITGA6 : ITGB1) is present in oocytes and is involved in sperm-egg fusion (By similarity). Integrin alpha-4/beta-1 is a receptor for VCAM1. It recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. Integrin alpha-3/beta-1 is a receptor for epiligrin, thrombospondin and CSPG4. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. Integrin alpha-V/beta-1 is a receptor for vitronectin. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. When associated with alpha-7 integrin, regulates cell adhesion and laminin matrix deposition. Involved in promoting endothelial cell motility and angiogenesis. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process and the formation of mineralized bone nodules. May be involved in up-regulation of the activity of kinases such as PKC via binding to KRT1. Together with KRT1 and RACK1, serves as a platform for SRC activation or inactivation. Plays a mechanistic adhesive role during telophase, required for the successful completion of cytokinesis. Integrin alpha-3/beta-1 provides a docking site for FAP (seprase) at invadopodia plasma membranes in a collagen-dependent manner and hence may participate in the adhesion, formation of invadopodia and matrix degradation processes, promoting cell invasion. ITGA4 : ITGB1 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling (PubMed : 23125415, PubMed : 24789099). ITGA4 : ITGB1 and ITGA5 : ITGB1 bind to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed : 18635536, PubMed : 25398877). ITGA5 : ITGB1 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1 (PubMed : 12807887, PubMed : 17158881). ITGA5 : ITGB1 acts as a receptor for fibronectin FN1 and mediates R-G-D-dependent cell adhesion to FN1 (PubMed : 33962943). ITGA5 : ITGB1 is a receptor for IL1B and binding is essential for IL1B signaling (PubMed : 29030430). ITGA5 : ITGB3 is a receptor for soluble CD40LG and is required for CD40/CD40LG signaling (PubMed : 31331973). Plays an important role in myoblast differentiation and fusion during skeletal myogenesis (By similarity). ITGA9 : ITGB1 may play a crucial role in SVEP1/polydom-mediated myoblast cell adhesion (By similarity). Integrins ITGA9 : ITGB1 and ITGA4 : ITGB1 repress PRKCA-mediated L-type voltage-gated channel Ca(2+) influx and ROCK-mediated calcium sensitivity in vascular smooth muscle cells via their interaction with SVEP1, thereby inhibit vasocontraction (PubMed : 35802072).. Isoform 2. Interferes with isoform 1 resulting in a dominant negative effect on cell adhesion and migration (in vitro).. Isoform 5. Isoform 5 displaces isoform 1 in striated muscles.. (Microbial infection) Integrin ITGA2 : ITGB1 acts as a receptor for Human echoviruses 1 and 8.. (Microbial infection) Acts as a receptor for Cytomegalovirus/HHV-5.. (Microbial infection) Acts as a receptor for Epstein-Barr virus/HHV-4.. (Microbial infection) Integrin ITGA5 : ITGB1 acts as a receptor for Human parvovirus B19.. (Microbial infection) Integrin ITGA2 : ITGB1 acts as a receptor for Human rotavirus.. (Microbial infection) Acts as a receptor for Mammalian reovirus.. (Microbial infection) In case of HIV-1 infection, integrin ITGA5 : ITGB1 binding to extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.. (Microbial infection) Interacts with CotH proteins expressed by fungi of the order mucorales, the causative agent of mucormycosis, which plays an important role in epithelial cell invasion by the fungi (PubMed : 32487760). Integrin ITGA3 : ITGB1 may act as a receptor for R.delemar CotH7 in alveolar epithelial cells, which may be an early step in pulmonary mucormycosis disease progression (PubMed : 32487760).. (Microbial infection) May serve as a receptor for adhesin A (nadA) of N.meningitidis.. (Microbial infection) Facilitates rabies infection in a fibronectin-dependent manner and participates in rabies virus traffic after internalization.

See full target information ITGB1

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Publications (43)

Recent publications for all applications. Explore the full list and refine your search

Cell reports. Medicine 6:102319 PubMed40882623

2025

Selenized neural stem cell-derived exosomes: A neotype therapeutic agent for traumatic injuries of the central nervous system.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjing Wang,Guihong Lu,Peilin Guo,Haochong Zhang,Yan Wang,Diwei Zheng,Chengliang Lyu,Dongfang Wang,Shang Li,Feng Li,Jiawei Zhao,Meng Qin,Weiping Li,Hui Tan,Guanghui Ma,Wei Wei

Frontiers in immunology 16:1577234 PubMed40475787

2025

Thrombospondin-1 mitigates osteoarthritis progression by inhibiting mechanical stress-induced chondrocyte ferroptosis via the integrin/YAP pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shaoyi Wang,Xiaocong Zhou,Fujian Zhang,Haoxin Zhai,Yuanqiang Zhang,Yongyuan Guo

Cell biology and toxicology 41:85 PubMed40369223

2025

IGFBP3 enhances adipose-derived stem cell function in soft tissue injury repair via ITGB1 and ERK pathway activation.

Applications

Unspecified application

Species

Unspecified reactive species

Sirui Tian,Haiyang Yu,Ruoxuan Yang,Heshi Wang,Baohong Zhao,Danning Wang

The Journal of cell biology 224: PubMed40304687

2025

Extracellular vesicles adhere to cells primarily by interactions of integrins and GM1 with laminin.

Applications

Unspecified application

Species

Unspecified reactive species

Tatsuki Isogai,Koichiro M Hirosawa,Miki Kanno,Ayano Sho,Rinshi S Kasai,Naoko Komura,Hiromune Ando,Keiko Furukawa,Yuhsuke Ohmi,Koichi Furukawa,Yasunari Yokota,Kenichi G N Suzuki

Materials today. Bio 28:101222 PubMed39296357

2024

A biomimetic targeted nanosystem delivering synergistic inhibitors for glioblastoma immune microenvironment reprogramming and treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Yulei Mu,Zhen Zhang,Huiqun Zhou,Min Jin,Liang Ma,Bangheng Liu,Cheng Ma,Xu Hu,Yi Zhang,Dong-An Wang

Cancer cell international 24:290 PubMed39143566

2024

Does rAj-Tspin, a novel peptide from A. japonicus, exert antihepatocellular carcinoma effects via the ITGB1/ZYX/FAK/AKT signaling pathway?

Applications

Unspecified application

Species

Unspecified reactive species

Ying Che,Xiaolong Lu,Xueting Wang,Zhien Liu,Liyang Guan,Xin Li,Zaixing Du,Hang Ren,Jihong Wang,Zunchun Zhou,Li Lv

Nutrition & metabolism 21:53 PubMed39080739

2024

Inhibition of N-acetylglucosaminyltransferase V alleviates diabetic cardiomyopathy in mice by attenuating cardiac hypertrophy and fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Ran Zhao,Jianqiang Hu,He Wen,Jieqiong Zhao,Ying Wang,Xiaona Niu,Mingming Zhang,Tingting Wang,Yan Li

Bioactive materials 38:399-410 PubMed38774457

2024

HtrA3 paves the way for MSC migration and promotes osteogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Yaru Guo,Siqin Ma,Dandan Wang,Feng Mei,Yusi Guo,Boon Chin Heng,Shihan Zhang,Ying Huang,Yan Wei,Ying He,Wenwen Liu,Mingming Xu,Xuehui Zhang,Lili Chen,Xuliang Deng

Cellular & molecular biology letters 29:46 PubMed38561669

2024

Sialylation on vesicular integrin β1 determined endocytic entry of small extracellular vesicles into recipient cells.

Applications

Unspecified application

Species

Unspecified reactive species

Meixuan Lin,Xiaoqiang Xu,Xiaoman Zhou,Hui Feng,Ruili Wang,Yunyun Yang,Jing Li,Ning Fan,Yazhuo Jiang,Xiang Li,Feng Guan,Zengqi Tan

Medicina (Kaunas, Lithuania) 60: PubMed38541090

2024

The Role of β1 Integrin/CD29 as a Potential Prognostic Factor for the Risk of Progression to Cervical Carcinoma in HPV-Associated Lesions.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Teresa Schettino,Eleonora Petra Preti,Valeria Vietri,Nadia Agrillo,Nicola Iavazzo,Diego Domenico Fasulo,Pasquale De Franciscis,Maria Rosaria Campitiello,Maria Giovanna Vastarella,Gaetano Riemma,Barbara Gardella,Filippo Murina

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Anti-Integrin beta 1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Integrin beta 1 antibody (AB183666)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 1 antibody (AB183666)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 1 antibody (AB183666)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 1 antibody (AB183666)

Immunocytochemistry/ Immunofluorescence - Anti-Integrin beta 1 antibody (AB183666)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 1 antibody (AB183666)

Immunoprecipitation - Anti-Integrin beta 1 antibody (AB183666)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 1 antibody (AB183666)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 1 antibody (AB183666)

Western blot - Anti-Integrin beta 1 antibody (AB183666)

Western blot - Anti-Integrin beta 1 antibody (AB183666)

Western blot - Anti-Integrin beta 1 antibody (AB183666)

Western blot - Anti-Integrin beta 1 antibody (AB183666)

Western blot - Anti-Integrin beta 1 antibody (AB183666)

Western blot - Anti-Integrin beta 1 antibody (AB183666)

Western blot - Anti-Integrin beta 1 antibody (AB183666)

Western blot - Anti-Integrin beta 1 antibody (AB183666)

Western blot - Anti-Integrin beta 1 antibody (AB183666)

Western blot - Anti-Integrin beta 1 antibody (AB183666)

Western blot - Anti-Integrin beta 1 antibody (AB183666)

Western blot - Anti-Integrin beta 1 antibody (AB183666)

Western blot - Anti-Integrin beta 1 antibody (AB183666)

Key facts

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Carrier free

Reacts with

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Immunogen

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Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

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Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

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Target data

Publications (43)

Product promise