AB7168

Anti-Integrin beta 1 antibody [BV7]

Name: Anti-Integrin beta 1 antibody [BV7] (ab7168) | Abcam
Brand: Abcam Limited
SKU: AB7168
Price: 565 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(17 Publications)

Mouse Monoclonal Integrin beta 1 antibody. Suitable for Flow Cyt (Intra) and reacts with Human samples. Cited in 17 publications. Immunogen corresponding to Cell preparation containing ITGB1 protein.

View Alternative Names

CD29, FNRB, MDF2, MSK12, ITGB1, Integrin beta-1, Fibronectin receptor subunit beta, Glycoprotein IIa, VLA-4 subunit beta, GPIIA

2 Images

Flow Cytometry (Intracellular) - Anti-Integrin beta 1 antibody [BV7] (AB7168)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Integrin beta 1 antibody [BV7] (AB7168)

Overlay histogram showing MCF-7 cells stained with ab7168 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7168, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG; H+L) (ab96879) at 1/250 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF-7 cells fixed with methanol (5 min) used under the same conditions.

Please note that Abcam does not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Integrin beta 1 antibody [BV7] (AB7168)

Overlay histogram showing HUVEC cells stained with ab7168 (blue line). Red line was isotype control mouse IgG₁. Black line was an irrelevant mouse IgG₁ used under the same conditions. Controls and anti-human ß1-integrin at 0.4 µg/100000 cells (1 hr 4°C).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

BV7

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra)

applications

Immunogen

Cell preparation containing ITGB1 protein. The exact immunogen used to generate this antibody is proprietary information.

P05556

Epitope

Antibody BV7 stains the extracellular domain of beta-1 integrin.

Specificity

This monoclonal antibody binds to human beta 1 integrin chain present on tumor cells. The antibody is active on HT-29 colon carcinoma cells and on HCCP-2998 tumor cells, as well as several other tumor cells (MG3 osteosarcoma, A375 melanoma, MHCC-1410 and Lovo colon carcinoma) but does not affect adhesion to endothelial cells.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1 µg/cells for 6 µg/cells", "FlowCytIntra-species-notes": "<p>(paraformaldehyde or methanol fixed cells).</p><p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.6 Constituents: PBS, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Integrin beta 1 also known as CD29 ITGB1 or integrin β1 is a part of the integrin family of cell surface receptors. This protein plays a role in cell adhesion and signal transduction processes. Integrin beta 1 weighs around 88 kDa and typically expresses on the surface of various cell types including epithelial endothelial and certain blood cells. As a transmembrane receptor it interacts with extracellular matrix components such as fibronectin collagen and laminin facilitating cell-matrix adhesion which is key in maintaining cellular structure and function.

Biological function summary

Integrin beta 1 functions in cells as a component of heterodimeric complexes with alpha integrin subunits forming adhesion receptors that mediate cellular signaling. These integrins are important for processes like cell migration differentiation proliferation and apoptosis. As they interact with various extracellular ligands integrin beta 1 influences numerous cellular responses particularly in tissue remodeling wound healing and embryonic development.

Pathways

Integrin beta 1 plays significant roles in the MAPK/ERK and PI3K/Akt signaling pathways both of which are essential for cellular growth and survival signals. It forms connections with associated proteins including focal adhesion kinase (FAK) and talin which participate in mechanotransduction and signal propagation. These interactions influence cellular responses to environmental changes impacting processes such as tissue repair and immune response modulation.

Integrin beta 1 associates with cancer progression and fibrosis disorders. Abnormal expression or function of integrin beta 1 can promote tumor invasion and metastasis making it a target for cancer research and potential therapeutic interventions. Furthermore in fibrosis integrin beta 1 interacts with transforming growth factor-beta (TGF-β) signaling contributing to excessive extracellular matrix production and scarring. Understanding these relationships helps in developing targeted treatments for conditions where integrin beta 1 plays a critical role.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta-1 and alpha-11/beta-1 are receptors for collagen. Integrins alpha-1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Integrins alpha-2/beta-1, alpha-3/beta-1, alpha-4/beta-1, alpha-5/beta-1, alpha-8/beta-1, alpha-10/beta-1, alpha-11/beta-1 and alpha-V/beta-1 are receptors for fibronectin. Alpha-4/beta-1 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. Integrin alpha-5/beta-1 is a receptor for fibrinogen. Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin. Integrin alpha-6/beta-1 (ITGA6 : ITGB1) is present in oocytes and is involved in sperm-egg fusion (By similarity). Integrin alpha-4/beta-1 is a receptor for VCAM1. It recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. Integrin alpha-3/beta-1 is a receptor for epiligrin, thrombospondin and CSPG4. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. Integrin alpha-V/beta-1 is a receptor for vitronectin. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. When associated with alpha-7 integrin, regulates cell adhesion and laminin matrix deposition. Involved in promoting endothelial cell motility and angiogenesis. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process and the formation of mineralized bone nodules. May be involved in up-regulation of the activity of kinases such as PKC via binding to KRT1. Together with KRT1 and RACK1, serves as a platform for SRC activation or inactivation. Plays a mechanistic adhesive role during telophase, required for the successful completion of cytokinesis. Integrin alpha-3/beta-1 provides a docking site for FAP (seprase) at invadopodia plasma membranes in a collagen-dependent manner and hence may participate in the adhesion, formation of invadopodia and matrix degradation processes, promoting cell invasion. ITGA4 : ITGB1 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling (PubMed : 23125415, PubMed : 24789099). ITGA4 : ITGB1 and ITGA5 : ITGB1 bind to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed : 18635536, PubMed : 25398877). ITGA5 : ITGB1 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1 (PubMed : 12807887, PubMed : 17158881). ITGA5 : ITGB1 acts as a receptor for fibronectin FN1 and mediates R-G-D-dependent cell adhesion to FN1 (PubMed : 33962943). ITGA5 : ITGB1 is a receptor for IL1B and binding is essential for IL1B signaling (PubMed : 29030430). ITGA5 : ITGB3 is a receptor for soluble CD40LG and is required for CD40/CD40LG signaling (PubMed : 31331973). Plays an important role in myoblast differentiation and fusion during skeletal myogenesis (By similarity). ITGA9 : ITGB1 may play a crucial role in SVEP1/polydom-mediated myoblast cell adhesion (By similarity). Integrins ITGA9 : ITGB1 and ITGA4 : ITGB1 repress PRKCA-mediated L-type voltage-gated channel Ca(2+) influx and ROCK-mediated calcium sensitivity in vascular smooth muscle cells via their interaction with SVEP1, thereby inhibit vasocontraction (PubMed : 35802072).. Isoform 2. Interferes with isoform 1 resulting in a dominant negative effect on cell adhesion and migration (in vitro).. Isoform 5. Isoform 5 displaces isoform 1 in striated muscles.. (Microbial infection) Integrin ITGA2 : ITGB1 acts as a receptor for Human echoviruses 1 and 8.. (Microbial infection) Acts as a receptor for Cytomegalovirus/HHV-5.. (Microbial infection) Acts as a receptor for Epstein-Barr virus/HHV-4.. (Microbial infection) Integrin ITGA5 : ITGB1 acts as a receptor for Human parvovirus B19.. (Microbial infection) Integrin ITGA2 : ITGB1 acts as a receptor for Human rotavirus.. (Microbial infection) Acts as a receptor for Mammalian reovirus.. (Microbial infection) In case of HIV-1 infection, integrin ITGA5 : ITGB1 binding to extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.. (Microbial infection) Interacts with CotH proteins expressed by fungi of the order mucorales, the causative agent of mucormycosis, which plays an important role in epithelial cell invasion by the fungi (PubMed : 32487760). Integrin ITGA3 : ITGB1 may act as a receptor for R.delemar CotH7 in alveolar epithelial cells, which may be an early step in pulmonary mucormycosis disease progression (PubMed : 32487760).. (Microbial infection) May serve as a receptor for adhesin A (nadA) of N.meningitidis.

See full target information ITGB1

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Publications (17)

Recent publications for all applications. Explore the full list and refine your search

PloS one 15:e0220019 PubMed31945053

2020

The use of mixed collagen-Matrigel matrices of increasing complexity recapitulates the biphasic role of cell adhesion in cancer cell migration: ECM sensing, remodeling and forces at the leading edge of cancer invasion.

Applications

Unspecified application

Species

Unspecified reactive species

María Anguiano,Xabier Morales,Carlos Castilla,Alejandro Rodríguez Pena,Cristina Ederra,Martín Martínez,Mikel Ariz,Maider Esparza,Hippolyte Amaveda,Mario Mora,Nieves Movilla,José Manuel García Aznar,Iván Cortés-Domínguez,Carlos Ortiz-de-Solorzano

International journal of molecular sciences 20: PubMed31261783

2019

Autocrine Production of PDGF Stimulated by the Tenascin-C-Derived Peptide TNIIIA2 Induces Hyper-Proliferation in Glioblastoma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Motomichi Fujita,Tetsuya Yamamoto,Takuya Iyoda,Tatsuya Fujisawa,Reo Nagai,Chikako Kudo,Manabu Sasada,Hiroaki Kodama,Fumio Fukai

Proceedings of the National Academy of Sciences of 114:E8685-E8694 PubMed28973887

2017

Cross-activating c-Met/β1 integrin complex drives metastasis and invasive resistance in cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Arman Jahangiri,Alan Nguyen,Ankush Chandra,Maxim K Sidorov,Garima Yagnik,Jonathan Rick,Sung Won Han,William Chen,Patrick M Flanigan,Dina Schneidman-Duhovny,Smita Mascharak,Michael De Lay,Brandon Imber,Catherine C Park,Kunio Matsumoto,Kan Lu,Gabriele Bergers,Andrej Sali,William A Weiss,Manish K Aghi

PloS one 12:e0171417 PubMed28166248

2017

Characterization of three-dimensional cancer cell migration in mixed collagen-Matrigel scaffolds using microfluidics and image analysis.

Applications

Unspecified application

Species

Unspecified reactive species

María Anguiano,Carlos Castilla,Martin Maška,Cristina Ederra,Rafael Peláez,Xabier Morales,Gorka Muñoz-Arrieta,Maite Mujika,Michal Kozubek,Arrate Muñoz-Barrutia,Ana Rouzaut,Sergio Arana,José Manuel Garcia-Aznar,Carlos Ortiz-de-Solorzano

PloS one 11:e0164799 PubMed27736988

2016

Beneficial Effects of the Genus Aloe on Wound Healing, Cell Proliferation, and Differentiation of Epidermal Keratinocytes.

Applications

Species

Human

Mariko Moriyama,Hiroyuki Moriyama,Junki Uda,Hirokazu Kubo,Yuka Nakajima,Arisa Goto,Junji Akaki,Ikuyo Yoshida,Nobuya Matsuoka,Takao Hayakawa

Oncotarget 7:74189-74202 PubMed27713116

2016

Prostate-specific membrane antigen (PSMA) assembles a macromolecular complex regulating growth and survival of prostate cancer cells "in vitro" and correlating with progression "in vivo".

Applications

Unspecified application

Species

Unspecified reactive species

Maria Elisa Perico,Silvia Grasso,Matteo Brunelli,Guido Martignoni,Enrico Munari,Enrico Moiso,Giulio Fracasso,Tiziana Cestari,Hassan Y Naim,Vincenzo Bronte,Marco Colombatti,Dunia Ramarli

Scientific reports 6:27639 PubMed27375161

2016

ATRA modulates mechanical activation of TGF-β by pancreatic stellate cells.

Applications

Unspecified application

Species

Human

Muge Sarper,Ernesto Cortes,Tyler J Lieberthal,Armando Del Río Hernández

Biology open 5:875-82 PubMed27170254

2016

Quantitative analysis of 3D extracellular matrix remodelling by pancreatic stellate cells.

Applications

Unspecified application

Species

Unspecified reactive species

Benjamin K Robinson,Ernesto Cortes,Alistair J Rice,Muge Sarper,Armando Del Río Hernández

Arteriosclerosis, thrombosis, and vascular biology 36:122-33 PubMed26564819

2015

MFAP4 Promotes Vascular Smooth Muscle Migration, Proliferation and Accelerates Neointima Formation.

Applications

Unspecified application

Species

Unspecified reactive species

Anders Schlosser,Bartosz Pilecki,Line E Hemstra,Karin Kejling,Gudlaug B Kristmannsdottir,Helle Wulf-Johansson,Jesper B Moeller,Ernst-Martin Füchtbauer,Ole Nielsen,Katrine Kirketerp-Møller,Lalit K Dubey,Pernille B L Hansen,Jane Stubbe,Christoph Wrede,Jan Hegermann,Matthias Ochs,Birgit Rathkolb,Anja Schrewe,Raffi Bekeredjian,Eckhard Wolf,Valérie Gailus-Durner,Helmut Fuchs,Martin Hrabě de Angelis,Jes S Lindholt,Uffe Holmskov,Grith L Sorensen

Oncotarget 6:17981-8000 PubMed26046793

2015

Identification of TAX2 peptide as a new unpredicted anti-cancer agent.

Applications

Unspecified application

Species

Human

Albin Jeanne,Emilie Sick,Jérôme Devy,Nicolas Floquet,Nicolas Belloy,Louis Theret,Camille Boulagnon-Rombi,Marie-Danièle Diebold,Manuel Dauchez,Laurent Martiny,Christophe Schneider,Stéphane Dedieu

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Integrin beta 1 antibody [BV7]

Flow Cytometry (Intracellular) - Anti-Integrin beta 1 antibody [BV7] (AB7168)

Flow Cytometry (Intracellular) - Anti-Integrin beta 1 antibody [BV7] (AB7168)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Epitope

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (17)

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