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Rabbit Recombinant Monoclonal Integrin beta 1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 131 publications.


Images

Western blot - Anti-Integrin beta 1 antibody [EP1041Y] (AB52971), expandable thumbnail
  • Western blot - Anti-Integrin beta 1 antibody [EP1041Y] (AB52971), expandable thumbnail
  • Western blot - Anti-Integrin beta 1 antibody [EP1041Y] (AB52971), expandable thumbnail
  • Western blot - Anti-Integrin beta 1 antibody [EP1041Y] (AB52971), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 1 antibody [EP1041Y] (AB52971), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PFlow CytWBICC/IF
Human
Tested
Not recommended
Tested
Not recommended

Tested
Tested

Species
Human
Dilution info
1/50
Notes

Use of an HRP/AP polymerized secondary antibody is recommended.

For unpurified, use 1/250 - 1/500.

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

For unpurified, use 1/70 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/100000
Notes

For unpurified, use 1/500.

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

15 products for Alternative Product

Target data

Function

Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta-1 and alpha-11/beta-1 are receptors for collagen. Integrins alpha-1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Integrins alpha-2/beta-1, alpha-3/beta-1, alpha-4/beta-1, alpha-5/beta-1, alpha-8/beta-1, alpha-10/beta-1, alpha-11/beta-1 and alpha-V/beta-1 are receptors for fibronectin. Alpha-4/beta-1 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. Integrin alpha-5/beta-1 is a receptor for fibrinogen. Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin. Integrin alpha-6/beta-1 (ITGA6:ITGB1) is present in oocytes and is involved in sperm-egg fusion (By similarity). Integrin alpha-4/beta-1 is a receptor for VCAM1. It recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. Integrin alpha-3/beta-1 is a receptor for epiligrin, thrombospondin and CSPG4. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. Integrin alpha-V/beta-1 is a receptor for vitronectin. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. When associated with alpha-7 integrin, regulates cell adhesion and laminin matrix deposition. Involved in promoting endothelial cell motility and angiogenesis. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process and the formation of mineralized bone nodules. May be involved in up-regulation of the activity of kinases such as PKC via binding to KRT1. Together with KRT1 and RACK1, serves as a platform for SRC activation or inactivation. Plays a mechanistic adhesive role during telophase, required for the successful completion of cytokinesis. Integrin alpha-3/beta-1 provides a docking site for FAP (seprase) at invadopodia plasma membranes in a collagen-dependent manner and hence may participate in the adhesion, formation of invadopodia and matrix degradation processes, promoting cell invasion. ITGA4:ITGB1 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling (PubMed:23125415, PubMed:24789099). ITGA4:ITGB1 and ITGA5:ITGB1 bind to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed:18635536, PubMed:25398877). ITGA5:ITGB1 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1 (PubMed:12807887, PubMed:17158881). ITGA5:ITGB1 acts as a receptor for fibronectin FN1 and mediates R-G-D-dependent cell adhesion to FN1 (PubMed:33962943). ITGA5:ITGB1 is a receptor for IL1B and binding is essential for IL1B signaling (PubMed:29030430). ITGA5:ITGB3 is a receptor for soluble CD40LG and is required for CD40/CD40LG signaling (PubMed:31331973). Plays an important role in myoblast differentiation and fusion during skeletal myogenesis (By similarity). ITGA9:ITGB1 may play a crucial role in SVEP1/polydom-mediated myoblast cell adhesion (By similarity). Integrins ITGA9:ITGB1 and ITGA4:ITGB1 repress PRKCA-mediated L-type voltage-gated channel Ca(2+) influx and ROCK-mediated calcium sensitivity in vascular smooth muscle cells via their interaction with SVEP1, thereby inhibit vasocontraction (PubMed:35802072). Isoform 2. Interferes with isoform 1 resulting in a dominant negative effect on cell adhesion and migration (in vitro). Isoform 5. Isoform 5 displaces isoform 1 in striated muscles. (Microbial infection) Integrin ITGA2:ITGB1 acts as a receptor for Human echoviruses 1 and 8. (Microbial infection) Acts as a receptor for Cytomegalovirus/HHV-5. (Microbial infection) Acts as a receptor for Epstein-Barr virus/HHV-4. (Microbial infection) Integrin ITGA5:ITGB1 acts as a receptor for Human parvovirus B19. (Microbial infection) Integrin ITGA2:ITGB1 acts as a receptor for Human rotavirus. (Microbial infection) Acts as a receptor for Mammalian reovirus. (Microbial infection) In case of HIV-1 infection, integrin ITGA5:ITGB1 binding to extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. (Microbial infection) Interacts with CotH proteins expressed by fungi of the order mucorales, the causative agent of mucormycosis, which plays an important role in epithelial cell invasion by the fungi (PubMed:32487760). Integrin ITGA3:ITGB1 may act as a receptor for R.delemar CotH7 in alveolar epithelial cells, which may be an early step in pulmonary mucormycosis disease progression (PubMed:32487760). (Microbial infection) May serve as a receptor for adhesin A (nadA) of N.meningitidis.

Alternative names

CD29, FNRB, MDF2, MSK12, ITGB1, Integrin beta-1, Fibronectin receptor subunit beta, Glycoprotein IIa, VLA-4 subunit beta, GPIIA

Recommended products

Rabbit Recombinant Monoclonal Integrin beta 1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 131 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EP1041Y
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Integrin beta 1 also known as CD29 ITGB1 or integrin β1 is a part of the integrin family of cell surface receptors. This protein plays a role in cell adhesion and signal transduction processes. Integrin beta 1 weighs around 88 kDa and typically expresses on the surface of various cell types including epithelial endothelial and certain blood cells. As a transmembrane receptor it interacts with extracellular matrix components such as fibronectin collagen and laminin facilitating cell-matrix adhesion which is key in maintaining cellular structure and function.

Biological function summary

Integrin beta 1 functions in cells as a component of heterodimeric complexes with alpha integrin subunits forming adhesion receptors that mediate cellular signaling. These integrins are important for processes like cell migration differentiation proliferation and apoptosis. As they interact with various extracellular ligands integrin beta 1 influences numerous cellular responses particularly in tissue remodeling wound healing and embryonic development.

Pathways

Integrin beta 1 plays significant roles in the MAPK/ERK and PI3K/Akt signaling pathways both of which are essential for cellular growth and survival signals. It forms connections with associated proteins including focal adhesion kinase (FAK) and talin which participate in mechanotransduction and signal propagation. These interactions influence cellular responses to environmental changes impacting processes such as tissue repair and immune response modulation.

Associated diseases and disorders

Integrin beta 1 associates with cancer progression and fibrosis disorders. Abnormal expression or function of integrin beta 1 can promote tumor invasion and metastasis making it a target for cancer research and potential therapeutic interventions. Furthermore in fibrosis integrin beta 1 interacts with transforming growth factor-beta (TGF-β) signaling contributing to excessive extracellular matrix production and scarring. Understanding these relationships helps in developing targeted treatments for conditions where integrin beta 1 plays a critical role.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-Integrin beta 1 antibody [EP1041Y] (ab52971), expandable thumbnail

    Western blot - Anti-Integrin beta 1 antibody [EP1041Y] (ab52971)

    Lanes 1, 5 and 9: Wild-type HAP1 cell lysate (20 μg)
    Lanes 2, 6 and 10: Integrin beta 1 knockout HAP1 cell lysate (20 μg)
    Lanes 3, 7 and 11: U87-MG cell lysate (20 μg)
    Lanes 4, 8 and 12: A431 cell lysate (20 μg)
    Lanes 1, 2, 3 and 4: Green signal from target – ab52971 observed at 140 kDa
    Lanes 5, 6, 7 and 8: Red signal from loading control – Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa
    Lanes 9, 10, 11 and 12: Merged (red and green) signal

    ab52971 was shown to specifically react with Integrin beta 1 in wild-type HAP1 cells. No band was observed when Integrin beta 1 knockout samples were examined. Wild-type and Integrin beta 1 knockout samples were subjected to SDS-PAGE. ab52971 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were diluted 1/10,000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Integrin beta 1 antibody [EP1041Y] (ab52971)

    Predicted band size: 88 kDa

  • Western blot - Anti-Integrin beta 1 antibody [EP1041Y] (ab52971), expandable thumbnail

    Western blot - Anti-Integrin beta 1 antibody [EP1041Y] (ab52971)

    Lane 1: Wild-type HAP1 cell lysate (20 μg)
    Lane 2: Integrin beta 1 knockout HAP1 cell lysate (20 μg)
    Lane 3: U87-MG cell lysate (20 μg)
    Lane 4: A431 cell lysate (20 μg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab52971 observed at 140 kDa. Red signal from loading control – Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa.

    This western blot image is a comparison between ab52971 and a competitor's top cited rabbit polyclonal antibody.

    All lanes: Western blot - Anti-Integrin beta 1 antibody [EP1041Y] (ab52971)

    Predicted band size: 88 kDa

  • Western blot - Anti-Integrin beta 1 antibody [EP1041Y] (ab52971), expandable thumbnail

    Western blot - Anti-Integrin beta 1 antibody [EP1041Y] (ab52971)

    5% NFDM/TBST dilution buffer

    All lanes: Western blot - Anti-Integrin beta 1 antibody [EP1041Y] (ab52971) at 1/10000 dilution

    Lane 1: U87-MG cell lysate at 20 µg

    Lane 2: HT-1080 cell lysate at 20 µg

    Lane 3: U937 cell lysate at 20 µg

    Secondary

    All lanes: HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 88 kDa

    Observed band size: 140 kDa

  • Western blot - Anti-Integrin beta 1 antibody [EP1041Y] (ab52971), expandable thumbnail

    Western blot - Anti-Integrin beta 1 antibody [EP1041Y] (ab52971)

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab52971 overnight at 4°C. Antibody binding was detected using Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) ab175781 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

    Secondary antibody - anti-rabbit Alexa Fluor 790. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) ab175781.

    All lanes: Western blot - Anti-Integrin beta 1 antibody [EP1041Y] (ab52971) at 20 µg

    Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

    Lane 2: HT 1080 (Human fibrosarcoma) Whole Cell Lysate at 20 µg

    Lane 3: U2OS (Human osteosarcoma cell line) Whole Cell Lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) ab175781) at 1/10000 dilution

    Predicted band size: 88 kDa

    Observed band size: 120 kDa, 140 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 1 antibody [EP1041Y] (ab52971), expandable thumbnail
    This image is courtesy of an anonymous customer review

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 1 antibody [EP1041Y] (ab52971)

    Unpurified ab52971 staining human breast cancer metastasis tissue sections by IHC-P. Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval in citrate buffer (pH 6) prior to blocking with a commercial blocking reagent and incubation with the antibody (diluted 1/100) for 18 hours at 4°C. A HRP-conjugated goat anti-rabbit was used as the secondary antibody. This image shows a cancer metastasis at 40x with beta1 staining (in red) in both blood vessels and tumour cells. Blue is Hoechst for nuclei. The antibody detection was enhanced using a commercial Cy3 tyramide signal amplification kit.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 1 antibody [EP1041Y] (ab52971), expandable thumbnail
    This image is courtesy of a customer review submitted by Mark Southwood.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 1 antibody [EP1041Y] (ab52971)

    Formaldehyde-fixed, paraffin-embedded human lung tissue stained for Integrin beta 1 using ab52971 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 1 antibody [EP1041Y] (ab52971), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 1 antibody [EP1041Y] (ab52971)

    ab52971 at 1/500 staining Integrin beta 1 antibody in human transitional cell carcinoma of bladder by immunohistochemistry (FFPE).
    Immunohistochemical analysis of paraffin-embedded human transitional cell carcinoma of bladder tissue labeling Integrin beta 1 with ab52971 at 1/500 dilution followed by goat anti-rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051, 1/500). Counter stained with hematoxylin.
    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

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