Anti-Integrin beta 1 antibody [EPR16895] - Low endotoxin, Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 1 antibody [EPR16895] - Low endotoxin, Azide free (AB221776)

Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Integrin beta 1 with ab179471 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Membrane staining on tumor cells of Human hepatocellular carcinoma is observed. Counter stained with Hematoxylin.

Negative control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS BSA glycerol and sodium azide (ab179471).

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 1 antibody [EPR16895] - Low endotoxin, Azide free (AB221776)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Integrin beta 1 with ab179471 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Membrane staining on epithelial cells of Human colon is observed. Counter stained with Hematoxylin.

Negative control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS BSA glycerol and sodium azide (ab179471).

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 1 antibody [EPR16895] - Low endotoxin, Azide free (AB221776)

Immunohistochemical analysis of paraffin-embedded Rat stomach tissue labeling Integrin beta 1 with ab179471 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Membrane staining on epithelial cells of rat stomach is observed. Counter stained with Hematoxylin.

Negative control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS BSA glycerol and sodium azide (ab179471).

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 1 antibody [EPR16895] - Low endotoxin, Azide free (AB221776)

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Integrin beta 1 with ab179471 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Membrane and cytoplasmic staining on mouse kidney is observed. Counter stained with Hematoxylin.

Negative control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS BSA glycerol and sodium azide (ab179471).

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• WB

Lab

Western blot - Anti-Integrin beta 1 antibody [EPR16895] - Low endotoxin, Azide free (AB221776)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179471).

Lanes 1, 5 and 9 : Wild-type HAP1 cell lysate (20 μg)
Lanes 2, 6 and 10 : Integrin beta 1 knockout HAP1 cell lysate (20 μg)
Lanes 3, 7 and 11 : U87-MG cell lysate (20 μg)
Lanes 4, 8 and 12 : A431 cell lysate (20 μg)
Lanes 1, 2, 3 and 4 : Green signal from target – ab179471 observed at 140 kDa
Lanes 5, 6, 7 and 8 : Red signal from loading control – ab8245 observed at 37 kDa
Lanes 9, 10, 11 and 12 : Merged (red and green) signal

ab179471 was shown to react with Integrin beta 1 in wild-type HAP1 cells as well as additional cross-reactive bands. No bands were observed when Integrin beta 1 knockout samples were examined. Wild-type and Integrin beta 1 knockout samples were subjected to SDS-PAGE. ab179471 and ab8245 (loading control to GAPDH) were diluted 1/10,000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1hr at room temperature before imaging.

All lanes:

Western blot - Anti-Integrin beta 1 antibody [EPR16895] - Low endotoxin, Azide free (ab221776)

Predicted band size: 88 kDa

false

• WB

Lab

Western blot - Anti-Integrin beta 1 antibody [EPR16895] - Low endotoxin, Azide free (AB221776)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179471).

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : Integrin beta 1 knockout HAP1 cell lysate (20 μg)
Lane 3 : U87-MG cell lysate (20 μg)
Lane 4 : A431 cell lysate (20 μg)

Lanes 1 - 4 : Merged signal (red and green). Green - ab179471 observed at 140 kDa. Red signal from loading control – ab8245 observed at 37 kDa.

This western blot image is a comparison between ab179471 and a competitor's top cited rabbit polyclonal antibody.

false

• WB

Lab

Western blot - Anti-Integrin beta 1 antibody [EPR16895] - Low endotoxin, Azide free (AB221776)

Blocking/Dilution buffer : 5% NFDM/TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179471).

All lanes:

Western blot - Anti-Integrin beta 1 antibody [EPR16895] - Low endotoxin, Azide free (ab221776) at 1/20000 dilution

All lanes:

A431 (Human epidermoid carcinoma) whole cell lysates at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 88 kDa

Observed band size: 130-150 kDa

false

Exposure time: 1min

• WB

Lab

Western blot - Anti-Integrin beta 1 antibody [EPR16895] - Low endotoxin, Azide free (AB221776)

Blocking/Dilution buffer : 5% NFDM/TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179471).

All lanes:

U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysates at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 88 kDa

Observed band size: 130-150 kDa

false

• WB

Lab

Western blot - Anti-Integrin beta 1 antibody [EPR16895] - Low endotoxin, Azide free (AB221776)

Blocking/Dilution buffer : 5% NFDM/TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179471).

All lanes:

Human spleen lysates at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 88 kDa

Observed band size: 130-150 kDa

false

Exposure time: 1min

• WB

Lab

Western blot - Anti-Integrin beta 1 antibody [EPR16895] - Low endotoxin, Azide free (AB221776)

This data was developed using ab179471, the same antibody clone in a different buffer formulation.

Western blot : Anti-Integrin beta 1 antibody [EPR16895] ab179471 staining at 1/2000 dilution, shown in green; Mouse anti GAPDH ab8245 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 100-130 kDa in Wild-type A549 cell lysates with no signal observed at this size in ITGB1 knockout A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5pc BSA in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-Integrin beta 1 antibody [EPR16895] (<a href='/en-us/products/primary-antibodies/integrin-beta-1-antibody-epr16895-ab179471'>ab179471</a>) at 1/2000 dilution

Lane 1:

Wild-type A549 at 20 µg

Lane 2:

Western blot - Human ITGB1 knockout A549 cell line (<a href='/en-us/products/cell-lines/human-itgb1-knockout-a549-cell-line-ab286593'>ab286593</a>) at 20 µg

Lane 3:

Wild-type HCT 116 at 20 µg

Lane 4:

ITGB1 knockout HCT 116 cell line at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 88 kDa

Observed band size: 100-130 kDa,37 kDa

false

• WB

Lab

Western blot - Anti-Integrin beta 1 antibody [EPR16895] - Low endotoxin, Azide free (AB221776)

Blocking/Dilution buffer : 5% NFDM/TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179471).

Lane 1:

Mouse heart lysates at 10 µg

Lane 2:

Mouse kidney lysates at 10 µg

Lane 3:

Rat heart lysates at 10 µg

Lane 4:

Rat kidney lysates at 10 µg

Lane 5:

Rat spleen lysates at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 88 kDa

Observed band size: 130-150 kDa

false

Exposure time: 1min