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Rabbit Recombinant Monoclonal Integrin beta 1 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

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Images

Western blot - Anti-Integrin beta 1 antibody [EPR16896] (AB179472), expandable thumbnail
  • Western blot - Anti-Integrin beta 1 antibody [EPR16896] (AB179472), expandable thumbnail
  • Western blot - Anti-Integrin beta 1 antibody [EPR16896] (AB179472), expandable thumbnail
  • Western blot - Anti-Integrin beta 1 antibody [EPR16896] (AB179472), expandable thumbnail
  • Western blot - Anti-Integrin beta 1 antibody [EPR16896] (AB179472), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Mouse
Tested
Rat
Tested

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Associated Products

Select an associated product type

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Target data

Function

Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta-1 and alpha-11/beta-1 are receptors for collagen. Integrins alpha-1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Integrins alpha-2/beta-1, alpha-3/beta-1, alpha-4/beta-1, alpha-5/beta-1, alpha-8/beta-1, alpha-10/beta-1, alpha-11/beta-1 and alpha-V/beta-1 are receptors for fibronectin. Alpha-4/beta-1 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. Integrin alpha-5/beta-1 is a receptor for fibrinogen. Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin. Integrin alpha-6/beta-1 (ITGA6:ITGB1) is present in oocytes and is involved in sperm-egg fusion (By similarity). Integrin alpha-4/beta-1 is a receptor for VCAM1. It recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. Integrin alpha-3/beta-1 is a receptor for epiligrin, thrombospondin and CSPG4. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. Integrin alpha-V/beta-1 is a receptor for vitronectin. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. When associated with alpha-7 integrin, regulates cell adhesion and laminin matrix deposition. Involved in promoting endothelial cell motility and angiogenesis. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process and the formation of mineralized bone nodules. May be involved in up-regulation of the activity of kinases such as PKC via binding to KRT1. Together with KRT1 and RACK1, serves as a platform for SRC activation or inactivation. Plays a mechanistic adhesive role during telophase, required for the successful completion of cytokinesis. Integrin alpha-3/beta-1 provides a docking site for FAP (seprase) at invadopodia plasma membranes in a collagen-dependent manner and hence may participate in the adhesion, formation of invadopodia and matrix degradation processes, promoting cell invasion. ITGA4:ITGB1 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling (PubMed:23125415, PubMed:24789099). ITGA4:ITGB1 and ITGA5:ITGB1 bind to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed:18635536, PubMed:25398877). ITGA5:ITGB1 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1 (PubMed:12807887, PubMed:17158881). ITGA5:ITGB1 acts as a receptor for fibronectin FN1 and mediates R-G-D-dependent cell adhesion to FN1 (PubMed:33962943). ITGA5:ITGB1 is a receptor for IL1B and binding is essential for IL1B signaling (PubMed:29030430). ITGA5:ITGB3 is a receptor for soluble CD40LG and is required for CD40/CD40LG signaling (PubMed:31331973). Plays an important role in myoblast differentiation and fusion during skeletal myogenesis (By similarity). ITGA9:ITGB1 may play a crucial role in SVEP1/polydom-mediated myoblast cell adhesion (By similarity). Integrins ITGA9:ITGB1 and ITGA4:ITGB1 repress PRKCA-mediated L-type voltage-gated channel Ca(2+) influx and ROCK-mediated calcium sensitivity in vascular smooth muscle cells via their interaction with SVEP1, thereby inhibit vasocontraction (PubMed:35802072). Isoform 2. Interferes with isoform 1 resulting in a dominant negative effect on cell adhesion and migration (in vitro). Isoform 5. Isoform 5 displaces isoform 1 in striated muscles. (Microbial infection) Integrin ITGA2:ITGB1 acts as a receptor for Human echoviruses 1 and 8. (Microbial infection) Acts as a receptor for Cytomegalovirus/HHV-5. (Microbial infection) Acts as a receptor for Epstein-Barr virus/HHV-4. (Microbial infection) Integrin ITGA5:ITGB1 acts as a receptor for Human parvovirus B19. (Microbial infection) Integrin ITGA2:ITGB1 acts as a receptor for Human rotavirus. (Microbial infection) Acts as a receptor for Mammalian reovirus. (Microbial infection) In case of HIV-1 infection, integrin ITGA5:ITGB1 binding to extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. (Microbial infection) Interacts with CotH proteins expressed by fungi of the order mucorales, the causative agent of mucormycosis, which plays an important role in epithelial cell invasion by the fungi (PubMed:32487760). Integrin ITGA3:ITGB1 may act as a receptor for R.delemar CotH7 in alveolar epithelial cells, which may be an early step in pulmonary mucormycosis disease progression (PubMed:32487760). (Microbial infection) May serve as a receptor for adhesin A (nadA) of N.meningitidis.

Alternative names

CD29, FNRB, MDF2, MSK12, ITGB1, Integrin beta-1, Fibronectin receptor subunit beta, Glycoprotein IIa, VLA-4 subunit beta, GPIIA

Recommended products

Rabbit Recombinant Monoclonal Integrin beta 1 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR16896
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Integrin beta 1 also known as CD29 ITGB1 or integrin β1 is a part of the integrin family of cell surface receptors. This protein plays a role in cell adhesion and signal transduction processes. Integrin beta 1 weighs around 88 kDa and typically expresses on the surface of various cell types including epithelial endothelial and certain blood cells. As a transmembrane receptor it interacts with extracellular matrix components such as fibronectin collagen and laminin facilitating cell-matrix adhesion which is key in maintaining cellular structure and function.

Biological function summary

Integrin beta 1 functions in cells as a component of heterodimeric complexes with alpha integrin subunits forming adhesion receptors that mediate cellular signaling. These integrins are important for processes like cell migration differentiation proliferation and apoptosis. As they interact with various extracellular ligands integrin beta 1 influences numerous cellular responses particularly in tissue remodeling wound healing and embryonic development.

Pathways

Integrin beta 1 plays significant roles in the MAPK/ERK and PI3K/Akt signaling pathways both of which are essential for cellular growth and survival signals. It forms connections with associated proteins including focal adhesion kinase (FAK) and talin which participate in mechanotransduction and signal propagation. These interactions influence cellular responses to environmental changes impacting processes such as tissue repair and immune response modulation.

Associated diseases and disorders

Integrin beta 1 associates with cancer progression and fibrosis disorders. Abnormal expression or function of integrin beta 1 can promote tumor invasion and metastasis making it a target for cancer research and potential therapeutic interventions. Furthermore in fibrosis integrin beta 1 interacts with transforming growth factor-beta (TGF-β) signaling contributing to excessive extracellular matrix production and scarring. Understanding these relationships helps in developing targeted treatments for conditions where integrin beta 1 plays a critical role.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-Integrin beta 1 antibody [EPR16896] (ab179472), expandable thumbnail

    Western blot - Anti-Integrin beta 1 antibody [EPR16896] (ab179472)

    Lanes 1- 2: Merged signal (red and green). Green - ab179472 observed at 140-150 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) observed at 37 kDa.

    ab179472 was shown to react with ITGB1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab255354 (knockout cell lysate ab263847) was used. Wild-type HeLa and ITGB1 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab179472 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Integrin beta 1 antibody [EPR16896] (ab179472) at 1/1000 dilution

    Lane 1: Wild-type HeLa cell lysate at 20 µg

    Lane 2: ITGB1 knockout HeLa cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 130 kDa, 88 kDa

    Observed band size: 140-150 kDa

  • Western blot - Anti-Integrin beta 1 antibody [EPR16896] (ab179472), expandable thumbnail

    Western blot - Anti-Integrin beta 1 antibody [EPR16896] (ab179472)

    Lanes 1 - 2: Merged signal (red and green). Green - ab179472 observed at 130 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

    ab179472 was shown to react with Integrin beta 1 in wild-type HCT 116 cells in western blot with loss of signal observed in ITGB1 knockout cell line Human ITGB1 (CD29) knockout HCT116 cell line ab273724 (ITGB1 knockout cell lysate Human ITGB1 (CD29) knockout HCT116 cell lysate ab275251). Wild-type and ITGB1 knockout HCT 116 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab179472 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Integrin beta 1 antibody [EPR16896] (ab179472) at 1/1000 dilution

    Lane 1: Wild-type HCT116 cell lysate at 20 µg

    Lane 2: ITGB1 knockout HCT116 cell lysate at 20 µg

    Lane 2: Western blot - Human ITGB1 (CD29) knockout HCT116 cell line (Human ITGB1 (CD29) knockout HCT116 cell line ab273724)

    Performed under reducing conditions.

    Predicted band size: 88 kDa

    Observed band size: 130 kDa

  • Western blot - Anti-Integrin beta 1 antibody [EPR16896] (ab179472), expandable thumbnail

    Western blot - Anti-Integrin beta 1 antibody [EPR16896] (ab179472)

    Lanes 1 - 2: Merged signal (red and green). Green - ab179472 observed at 125 kDa. Red - loading control, Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484, observed at 37 kDa.


    ab179472 was shown to specifically react with Integrin beta 1 in wild-type HAP1 cells as signal was lost in Integrin beta 1 knockout cells. Wild-type and Integrin beta 1 knockout samples were subjected to SDS-PAGE. ab179472 and Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Integrin beta 1 antibody [EPR16896] (ab179472) at 1/1000 dilution

    Lane 1: Wild-type HAP1 whole cell lysate at 20 µg

    Lane 2: Integrin beta 1 knockout HAP1 whole cell lysate at 20 µg

    Predicted band size: 88 kDa

  • Western blot - Anti-Integrin beta 1 antibody [EPR16896] (ab179472), expandable thumbnail

    Western blot - Anti-Integrin beta 1 antibody [EPR16896] (ab179472)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 23444218 and 23444218).

    All lanes: Western blot - Anti-Integrin beta 1 antibody [EPR16896] (ab179472) at 1/1000 dilution

    Lane 1: Human fetal heart lysate at 10 µg

    Lane 2: Human fetal kidney lysate at 10 µg

    Lane 3: Human fetal spleen lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/100000 dilution

    Predicted band size: 68 kDa, 72 kDa, 81 kDa, 88 kDa

    Observed band size: 120 kDa, 125 kDa, 81 kDa

    Exposure time: 10s

  • Western blot - Anti-Integrin beta 1 antibody [EPR16896] (ab179472), expandable thumbnail

    Western blot - Anti-Integrin beta 1 antibody [EPR16896] (ab179472)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1, 2 and 3: 3 seconds; Lane 4: 3 minutes.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 23444218 and 23444218).

    All lanes: Western blot - Anti-Integrin beta 1 antibody [EPR16896] (ab179472) at 1/1000 dilution

    Lane 1: A431 (Human epidermoid carcinoma cell line) whole cell lysate at 20 µg

    Lane 2: U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate at 20 µg

    Lane 3: HT1080 (Human fibrosarcoma cell line) whole cell lysate at 20 µg

    Lane 4: U937 (Human histiocytic lymphoma cell line) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 88 kDa

    Observed band size: 125 kDa

  • Western blot - Anti-Integrin beta 1 antibody [EPR16896] (ab179472), expandable thumbnail

    Western blot - Anti-Integrin beta 1 antibody [EPR16896] (ab179472)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1 and 2: 3 minutes; Lane 3,4 and 5: 1 minute.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 23444218 and 23444218).

    All lanes: Western blot - Anti-Integrin beta 1 antibody [EPR16896] (ab179472) at 1/1000 dilution

    Lane 1: Rat kidney lysate at 10 µg

    Lane 2: Rat spleen lysate at 10 µg

    Lane 3: C6 (Rat glial tumor cell line) whole cell lysate at 10 µg

    Lane 4: PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg

    Lane 5: NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 11 kDa, 88 kDa

    Observed band size: 125 kDa

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