Rabbit Polyclonal Integrin beta 3 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 10 publications. Immunogen corresponding to Fusion protein corresponding to ITGB3, fused to ITGB3.
Images
Publications
Filter by
- Clinical Medicine Insights. Oncology 17:117955492311811892023Fucosyltransferase 5 Promotes the Proliferative and Migratory Properties of Intrahepatic Cholangiocarcinoma Cells via Regulating Protein Glycosylation Profiles.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJingheng Guo,Qian Cheng,Yongjian Li,Lingyu Tian,Delin Ma,Zhao Li,Jie Gao,Jiye ZhuPubMed 37435017
- Bioactive materials 7:364-3762021Matrix stiffness modulates tip cell formation through the p-PXN-Rac1-YAP signaling axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYaru Guo,Feng Mei,Ying Huang,Siqin Ma,Yan Wei,Xuehui Zhang,Mingming Xu,Ying He,Boon Chin Heng,Lili Chen,Xuliang DengPubMed 34466738
- Clinical and experimental reproductive medicine 48:132-1412021Detrimental effects of lipopolysaccharide on the attachment and outgrowth of various trophoblastic spheroids on human endometrial epithelial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWontae Kim,Jungwon Choi,Hyejin Yoon,Jaewang Lee,Jin Hyun JunPubMed 34078006
- Investigative ophthalmology & visual science 60:1776-17882019Effect of αvβ3 Integrin Expression and Activity on Intraocular Pressure.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJennifer A Faralli,Mark S Filla,Donna M PetersPubMed 31022732
- Journal of thrombosis and haemostasis : JTH 17:206-2192018A unique phenotype of acquired Glanzmann thrombasthenia due to non-function-blocking anti-αIIbβ3 autoantibodies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesK Akuta,H Kashiwagi,T Yujiri,N Nishiura,Y Morikawa,H Kato,S Honda,Y Kanakura,Y TomiyamaPubMed 30388316
- mAbs 10:1003-10172018T-DM1-resistant cells gain high invasive activity via EGFR and integrin cooperated pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYukinori Endo et. al.PubMed 30130447
- Reproductive biology and endocrinology : RB&E 16:602018Decreased MUC1 in endometrium is an independent receptivity marker in recurrent implantation failure during implantation window.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFangrong Wu et. al.PubMed 29929546
- Nature communications 9:20732018Integrin beta3 regulates clonality and fate of smooth muscle-derived atherosclerotic plaque cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAshish Misra et. al.PubMed 29802249
- PloS one 13:e01926652018Absence of a secondary glucocorticoid response in C57BL/6J mice treated with topical dexamethasone.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJennifer A Faralli et. al.PubMed 29499052
- Oncotarget 8:6258-62722016Precise integrin-targeting near-infrared imaging-guided surgical method increases surgical qualification of peritoneal carcinomatosis from gastric cancer in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaidong Cheng,Chongwei Chi,Wenting Shang,Sha Rengaowa,Jianxin Cui,Jinzuo Ye,Shixin Jiang,Yamin Mao,Caoting Zeng,Huiping Huo,Lin Chen,Jie TianPubMed 28009982
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Fusion protein corresponding to ITGB3, fused to ITGB3. The exact immunogen used to generate this antibody is proprietary information. Database link P05106
Consider this alternative
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/2000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/25 - 1/100 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
15 products for Alternative Product
Target data
Function
Integrin alpha-V/beta-3 (ITGAV:ITGB3) is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIb/beta-3 (ITGA2B:ITGB3) is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain (By similarity). Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen (PubMed:9111081). This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. Fibrinogen binding enhances SELP expression in activated platelets (By similarity). ITGAV:ITGB3 binds to fractalkine (CX3CL1) and acts as its coreceptor in CX3CR1-dependent fractalkine signaling (PubMed:23125415, PubMed:24789099). ITGAV:ITGB3 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed:20682778). ITGAV:ITGB3 binds to FGF1 and this binding is essential for FGF1 signaling (PubMed:18441324). ITGAV:ITGB3 binds to FGF2 and this binding is essential for FGF2 signaling (PubMed:28302677). ITGAV:ITGB3 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed:19578119). ITGAV:ITGB3 binds to IGF2 and this binding is essential for IGF2 signaling (PubMed:28873464). ITGAV:ITGB3 binds to IL1B and this binding is essential for IL1B signaling (PubMed:29030430). ITGAV:ITGB3 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed:18635536, PubMed:25398877). ITGAV:ITGB3 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1 (PubMed:12807887). In brain, plays a role in synaptic transmission and plasticity. Involved in the regulation of the serotonin neurotransmission, is required to localize to specific compartments within the synapse the serotonin receptor SLC6A4 and for an appropriate reuptake of serotonin. Controls excitatory synaptic strength by regulating GRIA2-containing AMPAR endocytosis, which affects AMPAR abundance and composition (By similarity). ITGAV:ITGB3 act as a receptor for CD40LG (PubMed:31331973). ITGAV:ITGB3 acts as a receptor for IBSP and promotes cell adhesion and migration to IBSP (PubMed:10640428). (Microbial infection) Integrin ITGAV:ITGB3 acts as a receptor for Herpes virus 8/HHV-8. (Microbial infection) Integrin ITGAV:ITGB3 acts as a receptor for Coxsackievirus A9. (Microbial infection) Acts as a receptor for Hantaan virus. (Microbial infection) Integrin ITGAV:ITGB3 acts as a receptor for Cytomegalovirus/HHV-5. (Microbial infection) Integrin ITGA5:ITGB3 acts as a receptor for Human metapneumovirus. (Microbial infection) Integrin ITGAV:ITGB3 acts aP05556s a receptor for Human parechovirus 1. (Microbial infection) Integrin ITGAV:ITGB3 acts as a receptor for West nile virus. (Microbial infection) In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
Alternative names
CD61, GP3A, ITGB3, Integrin beta-3, Platelet membrane glycoprotein IIIa, GPIIIa
Recommended products
Rabbit Polyclonal Integrin beta 3 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 10 publications. Immunogen corresponding to Fusion protein corresponding to ITGB3, fused to ITGB3.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Fusion protein corresponding to ITGB3, fused to ITGB3. The exact immunogen used to generate this antibody is proprietary information. Database link P05106
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Integrin beta 3 also known as ITGB3 beta 3 integrin or CD41/CD61 is a cell surface receptor that plays a role in cell adhesion and signal transduction. It is a member of the integrin family characterized by a heterodimer structure formed with an alpha subunit. This integrin has a molecular mass of approximately 88 kDa. Integrin beta 3 is expressed on platelets megakaryocytes and several other cell types such as endothelial cells and certain tumor cells.
- Biological function summary
Integrins serve as the mechanical link between the extracellular matrix and the cytoskeleton. Beta 3 integrin forms a complex with different alpha subunits most notably alpha IIb to function as a receptor for fibrinogen fibronectin and vitronectin. This receptor complex is essential for platelet aggregation and normal hemostasis. Dysregulation can lead to altered cellular responses impacting processes like immune response and tumor progression.
- Pathways
Integrin beta 3 participates in the signaling of outside-in and inside-out pathways that are fundamental in cellular dynamics. Of particular significance are the integrin-mediated signaling pathways and the TGF-beta signaling pathway which influence cellular proliferation and differentiation. Integrin beta 3 interacts with proteins such as talin and kindlin which are vital for activation and clustering of integrins during cell signaling processes.
- Associated diseases and disorders
Altered expression or function of integrin beta 3 is associated with bleeding disorders like Glanzmann's thrombasthenia where platelet aggregation is impaired. Additionally it plays a role in the progression of certain cancers due to its involvement in cell migration and invasion. The interaction of integrin beta 3 with proteins such as VEGF and MMPs is significant in the context of tumor angiogenesis and metastasis.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Western blot - Anti-Integrin beta 3 antibody (ab197662)
8% SDS-PAGE.
All lanes: Western blot - Anti-Integrin beta 3 antibody (ab197662) at 1/450 dilution
All lanes: Jurkat cell lysate at 40 µg
Predicted band size: 87 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 3 antibody (ab197662)
Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue labeling Integrin beta 3 with ab197662 at 1/20 dilution.
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-Integrin beta 3 antibody (ab197662)
Integrin beta 3 western blot using anti-Integrin beta 3 antibody ab197662. Publication image and figure legend from Cheng, H., Chi, C., et al., 2017, Oncotarget, PubMed 28009982.
ab197662 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab197662 please see the product overview.
Expression of β3 in corresponding cell lines and the structure of the probe RGD-ICG(A) Integrin subunit β3 has a higher expression level in the gastric cancer cell line SGC-7901, compared with the breast cancer cell line MDA-MB-231, which has a high expression level of integrin αvβ3, by using GAPDH as the internal reference. GES-1 has a expression level of integrin subunit β3 similar to the breast cancer cell line MCF-7, which has a low expression of integrin αvβ3; (B) the chemistry and 3D structure of the probe RGD-ICG.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com