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Rabbit Recombinant Monoclonal Integrin beta 3 antibody. Suitable for WB, Flow Cyt (Intra), IP and reacts with Mouse, Human, Rat samples. Cited in 31 publications.

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Images

Immunoprecipitation - Anti-Integrin beta 3 antibody [EPR2342] (AB119992), expandable thumbnail
  • Western blot - Anti-Integrin beta 3 antibody [EPR2342] (AB119992), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Integrin beta 3 antibody [EPR2342] (AB119992), expandable thumbnail
  • Western blot - Anti-Integrin beta 3 antibody [EPR2342] (AB119992), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBFlow Cyt (Intra)IP
Human
Tested
Tested
Tested
Mouse
Tested
Expected
Expected
Rat
Expected
Expected
Expected

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Rat
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/20
Notes

-

Expected
Expected

Species
Rat
Dilution info
1/20
Notes

-

Species
Mouse
Dilution info
1/20
Notes

-

Tested
Tested

Species
Human
Dilution info
1/20
Notes

-

Expected
Expected

Species
Rat
Dilution info
1/20
Notes

-

Species
Mouse
Dilution info
1/20
Notes

-

Associated Products

Select an associated product type

15 products for Alternative Product

1 product for Alternative Version

Target data

Function

Integrin alpha-V/beta-3 (ITGAV:ITGB3) is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIb/beta-3 (ITGA2B:ITGB3) is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain (By similarity). Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen (PubMed:9111081). This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. Fibrinogen binding enhances SELP expression in activated platelets (By similarity). ITGAV:ITGB3 binds to fractalkine (CX3CL1) and acts as its coreceptor in CX3CR1-dependent fractalkine signaling (PubMed:23125415, PubMed:24789099). ITGAV:ITGB3 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed:20682778). ITGAV:ITGB3 binds to FGF1 and this binding is essential for FGF1 signaling (PubMed:18441324). ITGAV:ITGB3 binds to FGF2 and this binding is essential for FGF2 signaling (PubMed:28302677). ITGAV:ITGB3 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed:19578119). ITGAV:ITGB3 binds to IGF2 and this binding is essential for IGF2 signaling (PubMed:28873464). ITGAV:ITGB3 binds to IL1B and this binding is essential for IL1B signaling (PubMed:29030430). ITGAV:ITGB3 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed:18635536, PubMed:25398877). ITGAV:ITGB3 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1 (PubMed:12807887). In brain, plays a role in synaptic transmission and plasticity. Involved in the regulation of the serotonin neurotransmission, is required to localize to specific compartments within the synapse the serotonin receptor SLC6A4 and for an appropriate reuptake of serotonin. Controls excitatory synaptic strength by regulating GRIA2-containing AMPAR endocytosis, which affects AMPAR abundance and composition (By similarity). ITGAV:ITGB3 act as a receptor for CD40LG (PubMed:31331973). ITGAV:ITGB3 acts as a receptor for IBSP and promotes cell adhesion and migration to IBSP (PubMed:10640428). (Microbial infection) Integrin ITGAV:ITGB3 acts as a receptor for Herpes virus 8/HHV-8. (Microbial infection) Integrin ITGAV:ITGB3 acts as a receptor for Coxsackievirus A9. (Microbial infection) Acts as a receptor for Hantaan virus. (Microbial infection) Integrin ITGAV:ITGB3 acts as a receptor for Cytomegalovirus/HHV-5. (Microbial infection) Integrin ITGA5:ITGB3 acts as a receptor for Human metapneumovirus. (Microbial infection) Integrin ITGAV:ITGB3 acts aP05556s a receptor for Human parechovirus 1. (Microbial infection) Integrin ITGAV:ITGB3 acts as a receptor for West nile virus. (Microbial infection) In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.

Alternative names

CD61, GP3A, ITGB3, Integrin beta-3, Platelet membrane glycoprotein IIIa, GPIIIa

Recommended products

Rabbit Recombinant Monoclonal Integrin beta 3 antibody. Suitable for WB, Flow Cyt (Intra), IP and reacts with Mouse, Human, Rat samples. Cited in 31 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR2342
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Integrin beta 3 also known as ITGB3 beta 3 integrin or CD41/CD61 is a cell surface receptor that plays a role in cell adhesion and signal transduction. It is a member of the integrin family characterized by a heterodimer structure formed with an alpha subunit. This integrin has a molecular mass of approximately 88 kDa. Integrin beta 3 is expressed on platelets megakaryocytes and several other cell types such as endothelial cells and certain tumor cells.

Biological function summary

Integrins serve as the mechanical link between the extracellular matrix and the cytoskeleton. Beta 3 integrin forms a complex with different alpha subunits most notably alpha IIb to function as a receptor for fibrinogen fibronectin and vitronectin. This receptor complex is essential for platelet aggregation and normal hemostasis. Dysregulation can lead to altered cellular responses impacting processes like immune response and tumor progression.

Pathways

Integrin beta 3 participates in the signaling of outside-in and inside-out pathways that are fundamental in cellular dynamics. Of particular significance are the integrin-mediated signaling pathways and the TGF-beta signaling pathway which influence cellular proliferation and differentiation. Integrin beta 3 interacts with proteins such as talin and kindlin which are vital for activation and clustering of integrins during cell signaling processes.

Associated diseases and disorders

Altered expression or function of integrin beta 3 is associated with bleeding disorders like Glanzmann's thrombasthenia where platelet aggregation is impaired. Additionally it plays a role in the progression of certain cancers due to its involvement in cell migration and invasion. The interaction of integrin beta 3 with proteins such as VEGF and MMPs is significant in the context of tumor angiogenesis and metastasis.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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4 product images

  • Immunoprecipitation - Anti-Integrin beta 3 antibody [EPR2342] (ab119992), expandable thumbnail

    Immunoprecipitation - Anti-Integrin beta 3 antibody [EPR2342] (ab119992)

    Purified ab119992 at 1:20 dilution (0.7μg) immunoprecipitating Integrin beta 3 in HUVEC whole cell lysate.


    Lane 1 (input): HUVEC (Human umbilical vein endothelial cell) whole cell lysate 10μg.
    Lane 2 (+): ab119992 + HUVEC whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab119992 in HUVEC whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) (1:1000 dilution) was used for Western blotting.


    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 110 kDa

    All lanes: Immunoprecipitation - Anti-Integrin beta 3 antibody [EPR2342] (ab119992)

    Predicted band size: 87 kDa

  • Western blot - Anti-Integrin beta 3 antibody [EPR2342] (ab119992), expandable thumbnail

    Western blot - Anti-Integrin beta 3 antibody [EPR2342] (ab119992)

    Integrin beta 3 Western blot staining using rabbit Anti-Integrin beta 3 antibody

    Anti-Integrin beta antibody [EPR20825] (Anti-Integrin beta antibody [EPR20825] ab210515) at 1/1000 dilution or Anti-Integrin beta 3 antibody [EPR2342] (ab119992) at 1/500.

    Comparison of staining with an antibody specific to Integrin beta 3 (ab119992).

    The Integrin beta 3 immunogen used for this product shares the following sequence identities with other Integrin beta proteins: beta 5 – 61%, beta 6 – 53%, beta 7 – 48%, beta 1 – 47%, beta 2 – 46%, beta 8 – 42%, beta 4 – 38%. Specific cross-reactivities have not been determined experimentally, but based on our data we expect that the antibody cross-reacts with 1 or more of these proteins.

    Exposure:

    For Anti-Integrin beta antibody [EPR20825] ab210515: 1, 2, 5 lanes: 3 s; 3, 4 lanes: 37 s; 6 lane: 3 min; 7 lane: 15 s; 8 lane: 114 s.

    For ab119992: 1, 2 lane: 10 s; 3~8 lanes: 3 min

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Lanes 1 - 8: Western blot - Anti-Integrin beta antibody [EPR20825] (Anti-Integrin beta antibody [EPR20825] ab210515) at 1/1000 dilution

    Lanes 1 - 8: Western blot - Anti-Integrin beta 3 antibody [EPR2342] (ab119992) at 1/500 dilution

    Lane 1: U-87MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysates at 20 µg

    Lane 2: HEL (Human Erythroleukemia erythroblast) whole cell lysates at 20 µg

    Lane 3: LnCaP (Human prostate carcinoma epithelial cell) whole cell lysates at 20 µg

    Lane 4: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 20 µg

    Lane 5: HUVEC (Human umbilical vein endothelial cell) whole cell lysates at 20 µg

    Lane 6: PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysates at 20 µg

    Lane 7: C2C12 (Mouse myoblasts myoblast) whole cell lysates at 20 µg

    Lane 8: Raw264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 87 kDa

    Observed band size: 110 kDa

  • Flow Cytometry (Intracellular) - Anti-Integrin beta 3 antibody [EPR2342] (ab119992), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Integrin beta 3 antibody [EPR2342] (ab119992)

    Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HUVEC (Human umbilical vein endothelial cell) cell line labeling Integrin beta 3 with ab119992 at 1/20 (red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), at 1/2000 dilution was used as the secondary antibody.

  • Western blot - Anti-Integrin beta 3 antibody [EPR2342] (ab119992), expandable thumbnail

    Western blot - Anti-Integrin beta 3 antibody [EPR2342] (ab119992)

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM/TBST.

    Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 was used as a loading control.

    LnCaP, HeLa and PC-3 are low expression cell lines ( PMID: 21867506, PMID: 25248000).
    Though there are publications stating expression of Integrin beta 3 in RAW264.7, ab119992 and Anti-Integrin beta 3 antibody [ERP17507] ab179473 show negative in Raw264.7.

    All lanes: Western blot - Anti-Integrin beta 3 antibody [EPR2342] (ab119992) at 1/1000 dilution

    Lane 1: U-87MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: HEL (Human Erythroleukemia erythroblast) whole cell lysate at 20 µg

    Lane 3: LnCaP (Human prostate carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 4: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 5: HUVEC (Human umbilical vein endothelial cell) whole cell lysate at 20 µg

    Lane 6: PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 7: C2C12 (Mouse myoblasts myoblast) whole cell lysate at 20 µg

    Lane 8: Raw264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 87 kDa

    Observed band size: 110 kDa

    Exposure time: 40s

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