AB239991

Anti-Integrin beta 3 antibody [EPR2342] - BSA and Azide free

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal Integrin beta 3 antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

CD61, GP3A, ITGB3, Integrin beta-3, Platelet membrane glycoprotein IIIa, GPIIIa

3 Images

Flow Cytometry (Intracellular) - Anti-Integrin beta 3 antibody [EPR2342] - BSA and Azide free (AB239991)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Integrin beta 3 antibody [EPR2342] - BSA and Azide free (AB239991)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HUVEC (Human umbilical vein endothelial cell) cell line labeling Integrin beta 3 with ab119992 at 1/20 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab119992).

Supplier Data

Immunoprecipitation - Anti-Integrin beta 3 antibody [EPR2342] - BSA and Azide free (AB239991)

This data was developed using ab119992, the same antibody clone in a different buffer formulation.
Purified ab119992 at 1 : 20 dilution (0.7μg) immunoprecipitating Integrin beta 3 in HUVEC whole cell lysate.

Lane 1 (input) : HUVEC (Human umbilical vein endothelial cell) whole cell lysate 10μg.
Lane 2 (+) : ab119992 + HUVEC whole cell lysate.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab119992 in HUVEC whole cell lysate.
VeriBlot for IP Detection Reagent (HRP)(ab131366) (1 : 1000 dilution) was used for Western blotting.

Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.
Observed band size : 110 kDa

All lanes:

Immunoprecipitation - Anti-Integrin beta 3 antibody [EPR2342] (<a href='/en-us/products/primary-antibodies/integrin-beta-3-antibody-epr2342-ab119992'>ab119992</a>)

Predicted band size: 87 kDa

false

Lab

Western blot - Anti-Integrin beta 3 antibody [EPR2342] - BSA and Azide free (AB239991)

Blocking buffer and concentration : 5% NFDM/TBST. Diluting buffer and concentration : 5% NFDM/TBST. ab181602 was used as a loading control. LnCaP, HeLa and PC-3 are low expression cell lines ( PMID : 21867506, PMID : 25248000). Though there are publications stating expression of Integrin beta 3 in RAW264.7, ab119992 and ab179473 show negative in Raw264.7. This data was developed using ab119992, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Integrin beta 3 antibody [EPR2342] (<a href='/en-us/products/primary-antibodies/integrin-beta-3-antibody-epr2342-ab119992'>ab119992</a>) at 1/1000 dilution

Lane 1:

U-87MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

HEL (Human Erythroleukemia erythroblast) whole cell lysate at 20 µg

Lane 3:

LnCaP (Human prostate carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 5:

HUVEC (Human umbilical vein endothelial cell) whole cell lysate at 20 µg

Lane 6:

PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 7:

C2C12 (Mouse myoblasts myoblast) whole cell lysate at 20 µg

Lane 8:

Raw264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 87 kDa

Observed band size: 110 kDa

false

Exposure time: 40s

Unconjugated

Anti-Integrin beta 3 antibody [EPR2342]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Integrin beta 3 antibody [EPR2342]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Integrin beta 3 antibody [EPR2342]
578 PE

PE Anti-Integrin beta 3 antibody [EPR2342]
660 APC

APC Anti-Integrin beta 3 antibody [EPR2342]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2342

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Rat, Mouse

Applications

IP, Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

ab239991 is the carrier-free version of ab119992.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Integrin beta 3 also known as ITGB3 beta 3 integrin or CD41/CD61 is a cell surface receptor that plays a role in cell adhesion and signal transduction. It is a member of the integrin family characterized by a heterodimer structure formed with an alpha subunit. This integrin has a molecular mass of approximately 88 kDa. Integrin beta 3 is expressed on platelets megakaryocytes and several other cell types such as endothelial cells and certain tumor cells.

Biological function summary

Integrins serve as the mechanical link between the extracellular matrix and the cytoskeleton. Beta 3 integrin forms a complex with different alpha subunits most notably alpha IIb to function as a receptor for fibrinogen fibronectin and vitronectin. This receptor complex is essential for platelet aggregation and normal hemostasis. Dysregulation can lead to altered cellular responses impacting processes like immune response and tumor progression.

Pathways

Integrin beta 3 participates in the signaling of outside-in and inside-out pathways that are fundamental in cellular dynamics. Of particular significance are the integrin-mediated signaling pathways and the TGF-beta signaling pathway which influence cellular proliferation and differentiation. Integrin beta 3 interacts with proteins such as talin and kindlin which are vital for activation and clustering of integrins during cell signaling processes.

Altered expression or function of integrin beta 3 is associated with bleeding disorders like Glanzmann's thrombasthenia where platelet aggregation is impaired. Additionally it plays a role in the progression of certain cancers due to its involvement in cell migration and invasion. The interaction of integrin beta 3 with proteins such as VEGF and MMPs is significant in the context of tumor angiogenesis and metastasis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Integrin alpha-V/beta-3 (ITGAV : ITGB3) is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIb/beta-3 (ITGA2B : ITGB3) is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain (By similarity). Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen (PubMed : 9111081). This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. Fibrinogen binding enhances SELP expression in activated platelets (By similarity). ITGAV : ITGB3 binds to fractalkine (CX3CL1) and acts as its coreceptor in CX3CR1-dependent fractalkine signaling (PubMed : 23125415, PubMed : 24789099). ITGAV : ITGB3 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed : 20682778). ITGAV : ITGB3 binds to FGF1 and this binding is essential for FGF1 signaling (PubMed : 18441324). ITGAV : ITGB3 binds to FGF2 and this binding is essential for FGF2 signaling (PubMed : 28302677). ITGAV : ITGB3 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed : 19578119). ITGAV : ITGB3 binds to IGF2 and this binding is essential for IGF2 signaling (PubMed : 28873464). ITGAV : ITGB3 binds to IL1B and this binding is essential for IL1B signaling (PubMed : 29030430). ITGAV : ITGB3 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed : 18635536, PubMed : 25398877). ITGAV : ITGB3 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1 (PubMed : 12807887). In brain, plays a role in synaptic transmission and plasticity. Involved in the regulation of the serotonin neurotransmission, is required to localize to specific compartments within the synapse the serotonin receptor SLC6A4 and for an appropriate reuptake of serotonin. Controls excitatory synaptic strength by regulating GRIA2-containing AMPAR endocytosis, which affects AMPAR abundance and composition (By similarity). ITGAV : ITGB3 act as a receptor for CD40LG (PubMed : 31331973). ITGAV : ITGB3 acts as a receptor for IBSP and promotes cell adhesion and migration to IBSP (PubMed : 10640428). Integrin ITGA2B : ITGB3 is also the receptor of the erythrocyte-specific ICAM4 ligand involved in heterotypic cell-cell adhesion between erythrocytes and activated platelets (PubMed : 12477717).. (Microbial infection) Integrin ITGAV : ITGB3 acts as a receptor for Herpes virus 8/HHV-8.. (Microbial infection) Integrin ITGAV : ITGB3 acts as a receptor for Coxsackievirus A9.. (Microbial infection) Acts as a receptor for Hantaan virus.. (Microbial infection) Integrin ITGAV : ITGB3 acts as a receptor for Cytomegalovirus/HHV-5.. (Microbial infection) Integrin ITGA5 : ITGB3 acts as a receptor for Human metapneumovirus.. (Microbial infection) Integrin ITGAV : ITGB3 acts aP05556s a receptor for Human parechovirus 1.. (Microbial infection) Integrin ITGAV : ITGB3 acts as a receptor for West nile virus.. (Microbial infection) In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.

See full target information ITGB3

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell division 20:22 PubMed41039618

2025

FGF4 drives tumor progression in triple-negative breast cancer via IL6/STAT3-mediated macrophage M2 polarization and immune suppression.

Applications

Unspecified application

Species

Unspecified reactive species

Xuanhe Zhang,Shushan Zhang,Yuanyuan Han

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Integrin beta 3 antibody [EPR2342] - BSA and Azide free

Flow Cytometry (Intracellular) - Anti-Integrin beta 3 antibody [EPR2342] - BSA and Azide free (AB239991)

Immunoprecipitation - Anti-Integrin beta 3 antibody [EPR2342] - BSA and Azide free (AB239991)

Western blot - Anti-Integrin beta 3 antibody [EPR2342] - BSA and Azide free (AB239991)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Properties and storage information

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Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise