AB179473

Anti-Integrin beta 3 antibody [ERP17507]

Name: Anti-Integrin beta 3 antibody [ERP17507] (ab179473) | Abcam
Brand: Abcam Limited
SKU: AB179473
Price: 260 USD
Availability: InStock
Rating: 5 (2 reviews)

20ul selling size
RabMAb
Recombinant
What is this?

(2 Reviews)

(19 Publications)

Anti-Integrin beta 3 antibody [ERP17507] (ab179473) is a rabbit monoclonal antibody detecting Integrin beta 3 in Western Blot, IHC-P. Suitable for Human, Mouse.

- Biophysical QC for unrivalled batch-batch consistency
- Over 10 publications

View Alternative Names

CD61, GP3A, ITGB3, Integrin beta-3, Platelet membrane glycoprotein IIIa, GPIIIa

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 3 antibody [ERP17507] (AB179473)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 3 antibody [ERP17507] (AB179473)

Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue labeling Integrin beta 3 with ab179473 at 1/800 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Membrane or cytoplasmic staining on tumor cells of Human thyroid cancer is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 3 antibody [ERP17507] (AB179473)

Immunohistochemical analysis of paraffin-embedded Human osteosarcomas tissue labeling Integrin beta 3 with ab179473 at 1/800 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Membrane staining on cells of Human osteosarcomas is observed; several multinucleated osteoclasts are positively stained (PMID : 15692727; PMID : 25534583).

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 3 antibody [ERP17507] (AB179473)

Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling Integrin beta 3 with ab179473 at 1/800 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Positive staining on platelets (PMID : 25356112) of Human spleen is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-Integrin beta 3 antibody [ERP17507] (AB179473)

Blocking/Dilution buffer : 5% NFDM/TBST.

The molecular weight observed is consistent with the description in the literature (PMID : 1710517).

All lanes:

Western blot - Anti-Integrin beta 3 antibody [ERP17507] (ab179473) at 1/5000 dilution

All lanes:

Human plasma at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 87 kDa

Observed band size: 97 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-Integrin beta 3 antibody [ERP17507] (AB179473)

Blocking/Dilution buffer : 5% NFDM/TBST.

The molecular weight observed is consistent with the description in the literature (PMID : 1710517).

All lanes:

Western blot - Anti-Integrin beta 3 antibody [ERP17507] (ab179473) at 1000 Cells

All lanes:

HEL 92.1.7 (Human bone marrow erythroleukemia cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 87 kDa

Observed band size: 97 kDa

false

Exposure time: 5s

Lab

Western blot - Anti-Integrin beta 3 antibody [ERP17507] (AB179473)

LnCaP, HeLa and PC-3 are low expression controls.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Integrin beta 3 antibody [ERP17507] (ab179473) at 1/500 dilution

Lane 1:

U-87MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysates at 20 µg

Lane 2:

HEL (Human Erythroleukemia erythroblast) whole cell lysates at 20 µg

Lane 3:

LnCaP (Human prostate carcinoma epithelial cell) whole cell lysates at 20 µg

Lane 4:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 20 µg

Lane 5:

HUVEC (Human umbilical vein endothelial cell) whole cell lysates at 20 µg

Lane 6:

PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysates at 20 µg

Lane 7:

C2C12 (Mouse myoblasts myoblast) whole cell lysates at 20 µg

Lane 8:

Raw264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates at 20 µg

Lane 9:

Mouse brain lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 87 kDa

Observed band size: 110 kDa

false

Exposure time: 10s

Carrier free

Anti-Integrin beta 3 antibody [ERP17507] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

ERP17507

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/800", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-Integrin beta 3 antibody [ERP17507] (ab179473) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human, Mouse samples.

What is the molecular weight of Integrin beta 3?
Anti-Integrin beta 3 [ERP17507] (ab179473) specifically detects a band for Integrin beta 3 (UniProt: P05106) at a molecular weight of 87kDa.

Trusted by the scientific community
Anti-Integrin beta 3 [ERP17507] (ab179473) was first used in a scientific publication in 2016 and has been cited over 10 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [ERP17507] also available for your convenience: ab179473, Carrier free - ab240214

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Integrin beta 3 also known as ITGB3 beta 3 integrin or CD41/CD61 is a cell surface receptor that plays a role in cell adhesion and signal transduction. It is a member of the integrin family characterized by a heterodimer structure formed with an alpha subunit. This integrin has a molecular mass of approximately 88 kDa. Integrin beta 3 is expressed on platelets megakaryocytes and several other cell types such as endothelial cells and certain tumor cells.

Biological function summary

Integrins serve as the mechanical link between the extracellular matrix and the cytoskeleton. Beta 3 integrin forms a complex with different alpha subunits most notably alpha IIb to function as a receptor for fibrinogen fibronectin and vitronectin. This receptor complex is essential for platelet aggregation and normal hemostasis. Dysregulation can lead to altered cellular responses impacting processes like immune response and tumor progression.

Pathways

Integrin beta 3 participates in the signaling of outside-in and inside-out pathways that are fundamental in cellular dynamics. Of particular significance are the integrin-mediated signaling pathways and the TGF-beta signaling pathway which influence cellular proliferation and differentiation. Integrin beta 3 interacts with proteins such as talin and kindlin which are vital for activation and clustering of integrins during cell signaling processes.

Altered expression or function of integrin beta 3 is associated with bleeding disorders like Glanzmann's thrombasthenia where platelet aggregation is impaired. Additionally it plays a role in the progression of certain cancers due to its involvement in cell migration and invasion. The interaction of integrin beta 3 with proteins such as VEGF and MMPs is significant in the context of tumor angiogenesis and metastasis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Integrin alpha-V/beta-3 (ITGAV : ITGB3) is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIb/beta-3 (ITGA2B : ITGB3) is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain (By similarity). Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen (PubMed : 9111081). This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. Fibrinogen binding enhances SELP expression in activated platelets (By similarity). ITGAV : ITGB3 binds to fractalkine (CX3CL1) and acts as its coreceptor in CX3CR1-dependent fractalkine signaling (PubMed : 23125415, PubMed : 24789099). ITGAV : ITGB3 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed : 20682778). ITGAV : ITGB3 binds to FGF1 and this binding is essential for FGF1 signaling (PubMed : 18441324). ITGAV : ITGB3 binds to FGF2 and this binding is essential for FGF2 signaling (PubMed : 28302677). ITGAV : ITGB3 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed : 19578119). ITGAV : ITGB3 binds to IGF2 and this binding is essential for IGF2 signaling (PubMed : 28873464). ITGAV : ITGB3 binds to IL1B and this binding is essential for IL1B signaling (PubMed : 29030430). ITGAV : ITGB3 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed : 18635536, PubMed : 25398877). ITGAV : ITGB3 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1 (PubMed : 12807887). In brain, plays a role in synaptic transmission and plasticity. Involved in the regulation of the serotonin neurotransmission, is required to localize to specific compartments within the synapse the serotonin receptor SLC6A4 and for an appropriate reuptake of serotonin. Controls excitatory synaptic strength by regulating GRIA2-containing AMPAR endocytosis, which affects AMPAR abundance and composition (By similarity). ITGAV : ITGB3 act as a receptor for CD40LG (PubMed : 31331973). ITGAV : ITGB3 acts as a receptor for IBSP and promotes cell adhesion and migration to IBSP (PubMed : 10640428). Integrin ITGA2B : ITGB3 is also the receptor of the erythrocyte-specific ICAM4 ligand involved in heterotypic cell-cell adhesion between erythrocytes and activated platelets (PubMed : 12477717).. (Microbial infection) Integrin ITGAV : ITGB3 acts as a receptor for Herpes virus 8/HHV-8.. (Microbial infection) Integrin ITGAV : ITGB3 acts as a receptor for Coxsackievirus A9.. (Microbial infection) Acts as a receptor for Hantaan virus.. (Microbial infection) Integrin ITGAV : ITGB3 acts as a receptor for Cytomegalovirus/HHV-5.. (Microbial infection) Integrin ITGA5 : ITGB3 acts as a receptor for Human metapneumovirus.. (Microbial infection) Integrin ITGAV : ITGB3 acts aP05556s a receptor for Human parechovirus 1.. (Microbial infection) Integrin ITGAV : ITGB3 acts as a receptor for West nile virus.. (Microbial infection) In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.

See full target information ITGB3

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Publications (19)

Recent publications for all applications. Explore the full list and refine your search

European journal of nuclear medicine and molecular imaging : PubMed40728741

2025

[Ga]Ga-NODAGA-RGD post MI reflects activated fibroblasts rather than angiogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Matti Raitza,Markus Wolfien,Heiko Lemcke,Ralf Gäbel,Praveen Vasudevan,Moritz Schweins,Tobias Lindner,Markus Joksch,Anna Schildt,Jens Kurth,Änne Glass,Alper Öner,Hüseyin Ince,Felix G Meinel,Bernd Joachim Krause,Brigitte Vollmar,Robert David,Cajetan Immanuel Lang

Frontiers in immunology 16:1577234 PubMed40475787

2025

Thrombospondin-1 mitigates osteoarthritis progression by inhibiting mechanical stress-induced chondrocyte ferroptosis via the integrin/YAP pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shaoyi Wang,Xiaocong Zhou,Fujian Zhang,Haoxin Zhai,Yuanqiang Zhang,Yongyuan Guo

American journal of cancer research 14:5680-5696 PubMed39803652

2025

NRP1 overexpression potentially enhances osimertinib resistance in NSCLC via activation of the PI3K/AKT signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Wang,Bao-Lan Wang,Li-Qun Zhou,Yu-Feng Wan,Yu-Long Zheng,Li-Yang Zhou,Ran Fu,Chun-Hua Ling

EJNMMI research 14:62 PubMed38967722

2024

Ultrasensitive detection of uveal melanoma using [F]AlF-NOTA-PRGD2 PET imaging.

Applications

Unspecified application

Species

Unspecified reactive species

Ling Wang,Xue Zhu,Yan Xue,Zhihong Huang,Wenjun Zou,Zhengwei Zhang,Mengxi Yu,Donghui Pan,Ke Wang

Communications biology 7:795 PubMed38951640

2024

PEX3 promotes regenerative repair after myocardial injury in mice through facilitating plasma membrane localization of ITGB3.

Applications

Unspecified application

Species

Unspecified reactive species

Jia-Teng Sun,Zi-Mu Wang,Liu-Hua Zhou,Tong-Tong Yang,Di Zhao,Yu-Lin Bao,Si-Bo Wang,Ling-Feng Gu,Jia-Wen Chen,Tian-Kai Shan,Tian-Wen Wei,Hao Wang,Qi-Ming Wang,Xiang-Qing Kong,Li-Ping Xie,Ai-Hua Gu,Yang Zhao,Feng Chen,Yong Ji,Yi-Qiang Cui,Lian-Sheng Wang

The American journal of pathology 194:180-194 PubMed38029923

2023

Multilamellated Basement Membranes in the Capillary Network of Alveolar Capillary Dysplasia.

Applications

Unspecified application

Species

Unspecified reactive species

Jan C Kamp,Lavinia Neubert,Jonas C Schupp,Peter Braubach,Christoph Wrede,Florian Laenger,Tim Salditt,Jakob Reichmann,Tobias Welte,Arjang Ruhparwar,Fabio Ius,Nicolaus Schwerk,Anke K Bergmann,Sandra von Hardenberg,Matthias Griese,Christina Rapp,Karen M Olsson,Jan Fuge,Da-Hee Park,Marius M Hoeper,Danny D Jonigk,Lars Knudsen,Mark P Kuehnel

Proceedings of the National Academy of Sciences of the United States of America 120:e2304288120 PubMed37844244

2023

Defined extracellular matrix compositions support stiffness-insensitive cell spreading and adhesion signaling.

Applications

Unspecified application

Species

Unspecified reactive species

James R W Conway,Aleksi Isomursu,Gautier Follain,Ville Härmä,Eva Jou-Ollé,Nicolas Pasquier,Eetu P O Välimäki,Juha K Rantala,Johanna Ivaska

International journal of molecular sciences 24: PubMed37628895

2023

Platelet-Derived Extracellular Vesicles as Lipid Carriers in Severe Allergic Inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Alba Couto-Rodriguez,Alma Villaseñor,Carmela Pablo-Torres,David Obeso,María Fernanda Rey-Stolle,Héctor Peinado,José Luis Bueno,Mar Reaño-Martos,Alfredo Iglesias Cadarso,Cristina Gomez-Casado,Coral Barbas,Domingo Barber,María M Escribese,Elena Izquierdo

International journal of molecular sciences 24: PubMed36982771

2023

Integrin β3-Mediated Cell Senescence Associates with Gut Inflammation and Intestinal Degeneration in Models of Alzheimer's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Tun,Evan J Wang,Zhenxiang Gao,Kathleen Lundberg,Rong Xu,Di Hu

eLife 12: PubMed36892272

2023

Interplay of adherens junctions and matrix proteolysis determines the invasive pattern and growth of squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Takuya Kato,Robert P Jenkins,Stefanie Derzsi,Melda Tozluoglu,Antonio Rullan,Steven Hooper,Raphaël A G Chaleil,Holly Joyce,Xiao Fu,Selvam Thavaraj,Paul A Bates,Erik Sahai

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Integrin beta 3 antibody [ERP17507]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 3 antibody [ERP17507] (AB179473)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 3 antibody [ERP17507] (AB179473)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 3 antibody [ERP17507] (AB179473)

Western blot - Anti-Integrin beta 3 antibody [ERP17507] (AB179473)

Western blot - Anti-Integrin beta 3 antibody [ERP17507] (AB179473)

Western blot - Anti-Integrin beta 3 antibody [ERP17507] (AB179473)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (19)

Product promise