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Mouse Monoclonal Integrin beta 3 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ITGB3.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 3 antibody [ITGB3/1713] (AB218435), expandable thumbnail

Publications

  • Cell proliferation 52:e125502018
    Role of miR-223-3p in pulmonary arterial hypertension via targeting ITGB3 in the ECM pathway.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Aijun Liu et. al.
    PubMed 30507047

Key facts

Isotype

IgG1

Host species

Mouse

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human ITGB3. Database link P05106

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-P
Human
Tested

Tested
Tested

Species

Human

Dilution info

1-2 µg/mL

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

1 products for Alternative Version

    7 products for Alternative Product

      Target data

      Function

      Integrin alpha-V/beta-3 (ITGAV:ITGB3) is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIb/beta-3 (ITGA2B:ITGB3) is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. Fibrinogen binding enhances SELP expression in activated platelets (By similarity). ITGAV:ITGB3 binds to fractalkine (CX3CL1) and acts as its coreceptor in CX3CR1-dependent fractalkine signaling (PubMed:23125415, PubMed:24789099). ITGAV:ITGB3 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed:20682778). ITGAV:ITGB3 binds to FGF1 and this binding is essential for FGF1 signaling (PubMed:18441324). ITGAV:ITGB3 binds to FGF2 and this binding is essential for FGF2 signaling (PubMed:28302677). ITGAV:ITGB3 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed:19578119). ITGAV:ITGB3 binds to IGF2 and this binding is essential for IGF2 signaling (PubMed:28873464). ITGAV:ITGB3 binds to IL1B and this binding is essential for IL1B signaling (PubMed:29030430). ITGAV:ITGB3 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed:18635536, PubMed:25398877). ITGAV:ITGB3 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1 (PubMed:12807887). In brain, plays a role in synaptic transmission and plasticity. Involved in the regulation of the serotonin neurotransmission, is required to localize to specific compartments within the synapse the serotonin receptor SLC6A4 and for an appropriate reuptake of serotonin. Controls excitatory synaptic strength by regulating GRIA2-containing AMPAR endocytosis, which affects AMPAR abundance and composition (By similarity). ITGAV:ITGB3 act as a receptor for CD40LG (PubMed:31331973).(Microbial infection) Integrin ITGAV:ITGB3 acts as a receptor for Herpes virus 8/HHV-8.(Microbial infection) Integrin ITGAV:ITGB3 acts as a receptor for Coxsackievirus A9.(Microbial infection) Acts as a receptor for Hantaan virus.(Microbial infection) Integrin ITGAV:ITGB3 acts as a receptor for Cytomegalovirus/HHV-5.(Microbial infection) Integrin ITGA5:ITGB3 acts as a receptor for Human metapneumovirus.(Microbial infection) Integrin ITGAV:ITGB3 acts aP05556s a receptor for Human parechovirus 1.(Microbial infection) Integrin ITGAV:ITGB3 acts as a receptor for West nile virus.(Microbial infection) In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.

      Alternative names

      Integrin beta-3, Platelet membrane glycoprotein IIIa, GPIIIa, GP3A, ITGB3

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      Mouse Monoclonal Integrin beta 3 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ITGB3.

      Alternative names

      Integrin beta-3, Platelet membrane glycoprotein IIIa, GPIIIa, GP3A, ITGB3

      Key facts

      Isotype

      IgG1

      Form

      Liquid

      Clonality

      Monoclonal

      Immunogen
      • Recombinant Full Length Protein corresponding to Human ITGB3. Database link P05106
      Clone number

      ITGB3/1713

      Purification technique

      Affinity purification Protein A/G

      Light chain type

      kappa

      Concentration
      Loading...

      Storage

      Shipped at conditions

      Blue Ice

      Appropriate short-term storage duration

      1-2 weeks

      Appropriate short-term storage conditions

      +4°C

      Appropriate long-term storage conditions

      -20°C

      Aliquoting information

      Upon delivery aliquot

      Storage information

      Avoid freeze / thaw cycle

      Notes

      Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

      If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

      Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

      Supplementary info

      Activity summary

      Integrin beta 3 also known as ITGB3 beta 3 integrin or CD41/CD61 is a cell surface receptor that plays a role in cell adhesion and signal transduction. It is a member of the integrin family characterized by a heterodimer structure formed with an alpha subunit. This integrin has a molecular mass of approximately 88 kDa. Integrin beta 3 is expressed on platelets megakaryocytes and several other cell types such as endothelial cells and certain tumor cells.

      Biological function summary

      Integrins serve as the mechanical link between the extracellular matrix and the cytoskeleton. Beta 3 integrin forms a complex with different alpha subunits most notably alpha IIb to function as a receptor for fibrinogen fibronectin and vitronectin. This receptor complex is essential for platelet aggregation and normal hemostasis. Dysregulation can lead to altered cellular responses impacting processes like immune response and tumor progression.

      Pathways

      Integrin beta 3 participates in the signaling of outside-in and inside-out pathways that are fundamental in cellular dynamics. Of particular significance are the integrin-mediated signaling pathways and the TGF-beta signaling pathway which influence cellular proliferation and differentiation. Integrin beta 3 interacts with proteins such as talin and kindlin which are vital for activation and clustering of integrins during cell signaling processes.

      Associated diseases and disorders

      Altered expression or function of integrin beta 3 is associated with bleeding disorders like Glanzmann's thrombasthenia where platelet aggregation is impaired. Additionally it plays a role in the progression of certain cancers due to its involvement in cell migration and invasion. The interaction of integrin beta 3 with proteins such as VEGF and MMPs is significant in the context of tumor angiogenesis and metastasis.

      Product promise

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      1 product image

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 3 antibody [ITGB3/1713] (ab218435), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 3 antibody [ITGB3/1713] (ab218435)

        Immunohistochemical analysis of formalin-fixed, paraffin-embedded human spleen tissue, labeling Integrin beta 3 with ab218435 at 2 μg/mL.

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      Product protocols

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