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AB256135

Anti-Integrin beta 3 antibody [Y2/51] - BSA and Azide free

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Mouse Recombinant Monoclonal antibody. Carrier free. Suitable for Flow Cyt and reacts with Human samples.
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Flow Cytometry - Anti-Integrin beta 3 antibody [Y2/51] - BSA and Azide free (AB256135)
  • Flow Cyt

Lab

Flow Cytometry - Anti-Integrin beta 3 antibody [Y2/51] - BSA and Azide free (AB256135)

This data was developed using ab9509, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of HeLa (Human cervix adenocarcinoma epithelial cell, Left) / HUVEC (Human umbilical vein endothelial cell, Right) cells labelling Integrin beta 3 with ab9509 at 1/1000 dilution (0.1 ug)/Red compared with a Mouse monoclonal IgG / Black isotype control and an unlabelled control (Cell without incubation with primary antibody and secondary antibody / Blue).

Secondary antibody was Goat anti mouse IgG (Alexa Fluor® 488, ab150113) used at 1/2000 dilution.

Negative control : HeLa.
Gated on viable cells.

Flow Cytometry - Anti-Integrin beta 3 antibody [Y2/51] - BSA and Azide free (AB256135)
  • Flow Cyt

Lab

Flow Cytometry - Anti-Integrin beta 3 antibody [Y2/51] - BSA and Azide free (AB256135)

This data was developed using ab9509, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling Integrin beta 3 with ab9509 at 1/1000 dilution (0.1 ug) / Right compared with a Mouse monoclonal IgG / Left.

Goat anti mouse IgG (Alexa Fluor® 488, ab150113) was used as the secondary antibody at 1/2000 dilution.

Cells were stained with mouse IgG or ab9509. Then stained with anti-CD41 conjugated to APC.
Gated on viable cells.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

Y2/51

Isotype

IgG1

Light chain type

kappa

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Integrin beta 3 also known as ITGB3 beta 3 integrin or CD41/CD61 is a cell surface receptor that plays a role in cell adhesion and signal transduction. It is a member of the integrin family characterized by a heterodimer structure formed with an alpha subunit. This integrin has a molecular mass of approximately 88 kDa. Integrin beta 3 is expressed on platelets megakaryocytes and several other cell types such as endothelial cells and certain tumor cells.
Biological function summary

Integrins serve as the mechanical link between the extracellular matrix and the cytoskeleton. Beta 3 integrin forms a complex with different alpha subunits most notably alpha IIb to function as a receptor for fibrinogen fibronectin and vitronectin. This receptor complex is essential for platelet aggregation and normal hemostasis. Dysregulation can lead to altered cellular responses impacting processes like immune response and tumor progression.

Pathways

Integrin beta 3 participates in the signaling of outside-in and inside-out pathways that are fundamental in cellular dynamics. Of particular significance are the integrin-mediated signaling pathways and the TGF-beta signaling pathway which influence cellular proliferation and differentiation. Integrin beta 3 interacts with proteins such as talin and kindlin which are vital for activation and clustering of integrins during cell signaling processes.

Altered expression or function of integrin beta 3 is associated with bleeding disorders like Glanzmann's thrombasthenia where platelet aggregation is impaired. Additionally it plays a role in the progression of certain cancers due to its involvement in cell migration and invasion. The interaction of integrin beta 3 with proteins such as VEGF and MMPs is significant in the context of tumor angiogenesis and metastasis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

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Product promise

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